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Chapter 34 : Carbohydrate Catabolism: Pathways and Regulation

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Abstract:

The central pathways of carbon metabolism are conserved in virtually all organisms, but details of specific biosynthetic and degradative pathways vary considerably between bacteria, plants, and animals. In and other staphylococcal species, relatively few molecular details are known about carbohydrate utilization, biosynthetic pathways, and nutritional requirements. The limited knowledge on sugar utilization systems is especially surprising, because was the first gram-positive bacterium in which the phosphoenolpyruvate (PEP)-dependent carbohydrate phosphotransferase system (PTS) was described. The phosphoryl-transfer chain begins with enzyme I (EI) and PEP and proceeds via the phosphocarrier protein HPr to the EIIA and EIIB domains of the PTS permeases. The uptake of glucose, mannose, mannitol, glucosamine, -acetylglucosamine, sucrose, lactose, galactose, and β-glucosides is reported to be PTS-dependent. Glucose-6-phosphate, produced by a glucose kinase, enters the EMP pathway, the main glycolytic pathway in staphylococci. Utilization of lactose and galactose in relies on the PTS-dependent uptake and phosphorylation of the sugars, resulting in lactose-6-phosphate and galactose-6-phosphate, respectively. The system consists of an EIICB enzyme, encoded by , and EIIA, encoded by , which together form the mannitol-specific PTS permease. The sucrose PTS permease, analyzed in and encoded by , is composed of fused EIIBC domains. Maltose utilization in is dependent on an α-glucosidase or maltase, whose gene, , is the second gene of the operon. The availability of carbohydrates, especially of glucose, leads to regulatory processes often referred to as glucose effect or carbon catabolite repression.

Citation: Brückner R, Rosenstein R. 2006. Carbohydrate Catabolism: Pathways and Regulation, p 427-433. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch34

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Staphylococcus aureus
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Carbohydrates
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Staphylococcus xylosus
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Image of FIGURE 1
FIGURE 1

Alternative lactose catabolic pathways in staphylococci. Transport of lactose and galactose and their catabolism are shown. In , lactose and galactose are transported by the PEP:carbohydrate PTS. Internalized lactose-6-phosphate is hydrolyzed by a phospho-β-galactosidase to galactose-6-phosphate and glucose. Galactose-6-phosphate is catabolized through the tagatose-6-phosphate pathway. This pathway most likely exists in staphylococci exhibiting lactose PTS activity. In , and probably other staphylococcal species that do not possess a lactose PTS, a permease is responsible for the transport of lactose. Galactose uptake has not been studied in these species. Nonphosphorylated lactose is hydrolyzed by a β-galactosidase to yield glucose and galactose. Galactose is likely catabolized through the Leloir pathway. Glucose-6-phosphate, produced by a glucose kinase, enters the EMP pathway, the main glycolytic pathway in staphylococci. Only the galactoside-specific genes and their encoded products are mentioned in the pathways. Abbreviations: CM, cytoplasmic membrane; EI, enzyme I; EIIA, lactose-specific enzyme IIA; EIICB, lactose-specific enzyme IICB; HPr, histidine-containing protein; β-Gal, β-galactosidase; P-β-Gal, phospho-β-galactosidase; G6P-Isomerase, galactose-6-phosphate isomerase; G1P-Uridyltransferase, galactose-1-phosphate uridyltransferase; T6P-Kinase, tagatose-6-phosphate kinase; T1,6DP-Aldolase, tagatose-1,6-diphosphate aldolase; UDP-Gal, UDP-galactose; UDPGlc, UDP-glucose; UDP-G4-Epimerase, UDP-galactose-4 epimerase; PEP, phosphoenolpyruvate; P, phosphate; DP, diphosphate.

Citation: Brückner R, Rosenstein R. 2006. Carbohydrate Catabolism: Pathways and Regulation, p 427-433. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch34
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Image of FIGURE 2
FIGURE 2

Glucose-mediated carbon catabolite repression by the catabolite control protein CcpA in staphylococci. The components involved in regulation are: CcpA, carbon catabolite control protein; GlkA, glucose kinase; GlcU, PTS-independent glucose uptake protein; HPr, general PTS phosphocarrier protein; HPrK, HPr kinase/P-HPr phosphorylase; GlcA/GlcB, glucose-specific PTS permeases; FDP, fructose-1,6-diphosphate. The general PTS protein EI, essential for the transport of all PTS sugars, is omitted for clarity. The thick arrow represents a gene that is subject to carbon catabolite repression by CcpA via (catabolite-responsive element) interaction. The position of the CcpA binding site in the promoter region is indicated. The double line marked with CM represents the cytoplasmic membrane. The fact that some proteins may only function as dimers or multimers is not depicted. ATCC 1228 has apparently only one glucose-specific PTS permease. Glucose may be internalized by PTS-dependent or -independent transport. The glycolytic intermediate FDP activates kinase activity of HPrK, which produces P-ser-HPr by ATP-dependent phosphorylation. P-ser-HPr acts as a corepressor for CcpA, enabling the regulator to bind specifically to sites. When P prevails over ATP and FDP, HPrK dephosphorylates P-ser-HPr.

Citation: Brückner R, Rosenstein R. 2006. Carbohydrate Catabolism: Pathways and Regulation, p 427-433. In Fischetti V, Novick R, Ferretti J, Portnoy D, Rood J (ed), Gram-Positive Pathogens, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816513.ch34
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