Chapter 23 : The Humoral Immune Response to

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Antibody specific for both bacterial polysaccharide and protein can be protective against extracellular bacteria, including . In this regard, polysaccharide- and protein-specific Ig isotype responses to the intact bacterium could more closely resemble those observed using conjugate vaccines consisting of covalently linked polysaccharide and protein, as opposed to purified antigens. It remains to be determined which B-cell subpopulation(s) plays the major role in IgG antipolysaccharide responses to intact extracellular bacteria, where the role may depend on cognate CD4-T-cell help. Dendritic cells (DCs) in turn can directly stimulate activated B cells to secrete Ig in vitro and can play an active role in stimulating in vivo T-cell-independent (TI) and T-cell-dependent (TD) antipolysaccharide, as well as antiprotein, responses to intact . In vivo studies so far suggest only a limited role for endogenous NK cells in augmenting a humoral immune response, by demonstrating their ability to selectively stimulate trinitrophenyl (TNP)-specific IgG2a in response to the soluble TI antigen TNP-LPS. It is demonstrated instead that a critical balance exists between signals mediated by derived pathogen-associated molecular patterns and DC-derived interleukin-10 (IL-10) for optimization of DC induction of an in vivo humoral immune response. induces the early release of proinflammatory cytokines, the anti-inflammatory cytokine IL-10, and the type 2 cytokine IL-4. The use of intact as an immunogen to study the regulation of in vivo antipolysaccharide and antiprotein responses has revealed levels of complexity and distinct mechanistic pathways not anticipated from studies using isolated soluble antigens.

Citation: Snapper W, Colino J, Khan A, Wu Z. 2004. The Humoral Immune Response to , p 367-381. In Tuomanen E, Mitchell T, Morrison D, Spratt B (ed), The Pneumococcus. ASM Press, Washington, DC. doi: 10.1128/9781555816537.ch23

Key Concept Ranking

Bacterial Proteins
Major Histocompatibility Complex
MHC Class II
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Regulation of antiprotein and antipolysaccharide responses

Citation: Snapper W, Colino J, Khan A, Wu Z. 2004. The Humoral Immune Response to , p 367-381. In Tuomanen E, Mitchell T, Morrison D, Spratt B (ed), The Pneumococcus. ASM Press, Washington, DC. doi: 10.1128/9781555816537.ch23

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