Chapter 42 : Mycoparasitism

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Mycoparasitism is considered a major contributor to fungus-fungus antagonism. The necrotrophs, primarily species, have a wider host range and less-specific mode of action, and perhaps for this reason more field and greenhouse trials have made use of these. species are focused in this chapter, because they have been the focus of the most work at the molecular and cellular levels. The interaction of with soilborne pathogenic fungi is an excellent example of necrotrophic mycoparasitism. Drastic reduction of intracellular cyclic AMP (cAMP) by knockout of adenylate cyclase leads to slow growth and loss of mycoparasitism in . In , mutants in the ortholog of the same mitogen-activated protein kinase (MAPK) gene, , showed increased coiling but reduced mycoparasitism in confrontation assays. A potentiation in the gene expression enables -treated plants to be more resistant to subsequent pathogen infection. Chytrids parasitizing vesicular arbuscular mycorrhizae were found in the fossil record of the Rhynie Chert, dating to more than 400 million years ago. There are theories that mycorrhizae themselves might have evolved from biotrophic fungal parasites of plants. Major features such as detection of the host, signal transduction, altered transcriptional patterns, and secretion of enzymes are likely to be shared, and this has provided working hypotheses to guide studies of mycoparasitism. Molecular mechanisms and genes manipulated to optimize biocontrol will be different for each type of interaction and for each niche within the complex web of fungus-fungus and fungus-root interactions.

Citation: Viterbo A, Horwitz B. 2010. Mycoparasitism, p 676-693. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch42

Key Concept Ranking

Plant Pathogenic Fungi
Trichoderma reesei
Trichoderma harzianum
Trichoderma virens
Trichoderma harzianum
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Image of FIGURE 1

Basic mechanisms of antagonistic fungus-fungus interactions. (A) Antibiosis: culture filtrate from inhibits germination of conidia. Shown are culture filtrates from grown on 2% glucose (left) and on 0.5% colloidal chitin (right). Growth of on chitin induces production of soluble antagonistic factors, which could be enzymes or small metabolites. (Reprinted from , with permission of Blackwell Publishing Ltd.) (B) Necrotrophic mycoparasitic interactions: coiling of the mycoparasite around the host, shown here, is followed by destruction of the host. Upper panel, on (from ); lower panel, hyphae interacting with f. sp. . (Reprinted with permission from ) (C) Parasitism of sclerotia: an important interaction for biocontrol of soilborne disease. Upper four panels, SEM images (reprinted from Mukherjee et al., 1995b, with permission of Blackwell Publishing Ltd.). Lower two panels, fluorescence of GFP-expressing mycelia; fluorescent mycelia are indicated by arrows (reprinted from , with permission from Elsevier). (D) A biotrophic interaction: the parasite forms haustoria within the host cells, as in biotrophic fungus-plant interactions ( ). Image reprinted from ) (Fig. 12-1), courtesy of Jim Deacon, The University of Edinburgh.

Citation: Viterbo A, Horwitz B. 2010. Mycoparasitism, p 676-693. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch42
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Image of FIGURE 2

Image of holes in host cell walls left by , evidence of digestion by secreted enzymes. (Reprinted with permission from .)

Citation: Viterbo A, Horwitz B. 2010. Mycoparasitism, p 676-693. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch42
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Image of FIGURE 3

Modulation of mycoparasite gene expression by host-derived signals. Expression of chit36:GFP in a transgenic line (T) induced by interaction with (Rs). was grown on a dialysis membrane on minimal medium for 2 days and then transferred for 24 h onto a clean plate (panel 1); a plate where was previously grown for 2 days (panel 2); or a 2-day-old culture of (panel 3). (Reprinted from , with permission of Springer Science and Business Media.)

Citation: Viterbo A, Horwitz B. 2010. Mycoparasitism, p 676-693. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch42
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Image of FIGURE 4

A model for how host signals are produced. Action of hydrolytic enzymes secreted by the mycoparasite releases diffusible products, and secondary metabolites of fungal origin (for example, 6-pentyl-2H-pyran-2-on3). These signals may, in turn, program the development of the mycoparasite. (Reprinted from , with permission from Elsevier.)

Citation: Viterbo A, Horwitz B. 2010. Mycoparasitism, p 676-693. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch42
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Image of FIGURE 5

Interactions with plant roots. (A) Left, interaction between (hypha indicated in SEM image by the arrow) and cucumber root. (Reprinted with permission from .) Right, interaction between tomato root and expressing GFP (fluorescent hyphae indicated by arrow). (Reprinted with permission from .) (B) Consequence of -root interactions: induction of systemic resistance in the plant. Bioassay for induced resistance in maize seedlings against . Left, symptoms on leaves of maize seedlings grown from untreated (NT) seeds, or seed treated with knockout strains (KO25 and KO46), wild-type strain (WT), or overexpression strains (OE38 and OE39) of . Lesions (indicated, for example, by black arrows in left-most leaf photo) appear dark in this grayscale image; see ) for original color image from which this figure was reprinted with permission. The gene disrupted or overexpressed in these strains encodes the secreted elicitor protein Sm1. Right, quantitative analysis of lesion size for the experiment shown in the left panel; different letters indicate significant differences ( ).

Citation: Viterbo A, Horwitz B. 2010. Mycoparasitism, p 676-693. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch42
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Summary of some mycoparasitic fungi applied in biocontrol or with potential as biocontrol agents

Citation: Viterbo A, Horwitz B. 2010. Mycoparasitism, p 676-693. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch42

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