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Abstract:

causes a spectrum of clinical entities, ranging from allergic rhinitis to invasive disease. Following the inhalation of conidia, allergic rhinitis and allergic bronchopulmonary aspergillosis (ABPA) result from overly exuberant immunological responses. Techniques for global analysis of transcription are revolutionizing one's understanding of fungal physiology, including the complex regulatory networks that facilitate adaptation to the host environment. The first large-scale analysis of the transcriptome was published in conjunction with the initial report of the Af293 genome sequence. In this study, microarrays were used to gain insight into genes that are differentially regulated in response to a temperature shift, with the goal of understanding aspects of thermotolerant growth that are relevant to mammalian infection. A more targeted proteomics strategy has been used to uncover novel cellulases in the proteome. The procedure focuses on the identification of β-glucosidase activity in the secreted proteome using a 2D-in-gel β-glucosidase activity assay combined with tandem mass spectrometry (MS). Programmed cell death (PCD) is an important stress response among metazoans, and components of the pathway are considered to be excellent targets for anticancer drug development. Typical primary pathogen has unique virulence traits that evolved in association with a host organism. These virulence factors are generally dispensable for growth outside the host but provide some competitive advantage to the organism when it is in the host environment.

Citation: Rhodes J, Askew D. 2010. , p 697-716. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch43

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Gene Expression and Regulation
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Allergic Bronchopulmonary Aspergillosis
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FIGURE 1

Schematic illustration of the life cycle and pathogenesis of (not drawn to scale). propagates itself in the environment by the release of asexual conidia into the atmosphere. The conidia begin the process of germination when they encounter a suitable substrate, forming mature hyphae that eventually develop into a mycelium. In response to an appropriate stimulus, such as nutrient deprivation, some of the hyphae activate the developmental program of asexual development, resulting in a series of morphological changes: an aerial hypha, or stalk, forms from a thick-walled foot-cell, and the stalk tip swells to form a vesicle. A single row of phialides then forms on the vesicle surface, from which the conidia subsequently develop. A mature conidiophore can possess up to 50,000 conidia, which are released into the environment in response to changes in airflow. The airborne conidia are inhaled by most people on a daily basis and are small enough (2 to 3 μm in diameter) to reach the distal airways. The conidia are efficiently cleared in a healthy individual, but in an immunodeficient host they are able to germinate into invasive hyphae that penetrate the vasculature and migrate to distal sites.

Citation: Rhodes J, Askew D. 2010. , p 697-716. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch43
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References

/content/book/10.1128/9781555816636.ch43
1. Alcazar-Fuoli, L.,, E. Mellado,, G. Garcia-Effron,, M. J. Buitrago,, J. F. Lopez,, J. O. Grimmalt,, J. M. Cuenca-Estrella, and, J. L. Rodriguez-Tudela. 2006. Aspergillus fumigatus C-5 sterol desaturases Erg3A and Erg3B: role in sterol biosynthesis and antifungal drug susceptibility. Antimicrob. Agents Chemother. 50:453460.
2. Anaissie, E. J.,, S. L. Stratton,, M. C. Dignani,, R. C. Summer-bell,, J. H. Rex,, T. P. Monson,, T. Spencer,, M. Kasai,, A. Francesconi, and, T. J. Walsh. 2002. Pathogenic Aspergillus species recovered from a hospital water system: a 3-year prospective study. Clin. Infect. Dis. 34:780789.
3. Antachopoulos, C.,, T. J. Walsh, and, E. Roilides. 2007. Fungal infections in primary immunodeficiencies. Eur. J. Pediatr. 166:10991117.
4. Araujo, R., and, A. G. Rodrigues. 2004. Variability of germinative potential among pathogenic species of Aspergillus. J. Clin. Microbiol. 42:43354337.
5. Aubry, A.,, R. Porcher,, J. Bottero,, S. Touratier,, T. Leblanc,, B. Brethon,, P. Rousselot,, E. Raffoux,, J. Menotti,, F. Derouin,, P. Ribaud, and, A. Sulahian. 2006. Occurrence and kinetics of false-positive Aspergillus galactomannan test results following treatment with beta-lactam antibiotics in patients with hematological disorders. J. Clin. Microbiol. 44:389394.
6. Aufauvre-Brown, A.,, J. S. Brown, and, D. W. Holden. 1998. Comparison of virulence between clinical and environmental isolates of Aspergillus fumigatus. Eur. J. Clin. Microbiol. Infect. Dis. 17:778780.
7. Aufauvre-Brown, A.,, E. Mellado,, N. A. R. Gow, and, D. W. Holden. 1997. Aspergillus fumigatus chsE: a gene related to CHS3 of Saccharomyces cerevisiae and important for hyphal growth and conidiophore development but not pathogenicity. Fungal Genet. Biol. 21:141152.
8. Balloy, V.,, M. Huerre,, J.-P. Latge, and, M. Chignard. 2005. Differences in patterns of infection and inflammation for corticosteroid treatment and chemotherapy in experimental invasive pulmonary aspergillosis. Infect. Immun. 73:494503.
9. Bammert, G. F., and, J. M. Fostel. 2000. Genome-wide expression patterns in Saccharomyces cerevisiae: comparison of drug treatments and genetic alterations affecting biosynthesis of ergosterol. Antimicrob. Agents Chemother. 44:12551265.
10. Beauvais, A.,, D. Maubon,, S. Park,, W. Morelle,, M. Tanguy,, M. Huerre,, D. S. Perlin, and, J. P. Latge. 2005. Two alpha(1-3) glucan synthases with different functions in Aspergillus fumigatus. Appl. Environ. Microbiol. 71:15311538.
11. Bellocchio, S.,, S. Moretti,, K. Perruccio,, F. Fallarino,, S. Bozza,, C. Montagnoli,, P. Mosci,, G. B. Lipford,, L. Pitzurra, and, L. Romani. 2004. TLRs govern neutrophil activity in aspergillosis. J. Immunol. 173:74067415.
12. Bhabhra, R.,, M. D. Miley,, E. Mylonakis,, D. Boettner,, J. Fortwendel,, J. C. Panepinto,, M. Postow,, J. C. Rhodes, and, D. S. Askew. 2004. Disruption of the Aspergillus fumigatus gene encoding nucleolar protein CgrA impairs thermotolerant growth and reduces virulence. Infect. Immun. 72:47314740.
13. Bhabhra, R.,, D. L. Richie,, H. S. Kim,, W. C. Nierman,, J. Fortwendel,, J. P. Aris,, J. C. Rhodes, and, D. S. Askew. 2008. Impaired ribosome biogenesis disrupts the integration between morphogenesis and nuclear duplication during the germination of Aspergillus fumigatus. Eukaryot. Cell 7:575583.
14. Bodey, G.,, B. Bueltmann,, W. Duguid,, D. Gibbs,, H. Hanak,, M. Hotchi,, G. Mall,, P. Martino,, F. Meunier,, S. Milliken,, S. Naoe,, M. Okudaira,, D. Scevola, and, J. van’tWout. 1992. Fungal infection in cancer patients: an international autopsy survey. Eur. J. Clin. Microbiol. Infect. Dis. 11:99109.
15. Bok, J. W.,, S. A. Balajee,, K. A. Marr,, D. Andes,, K. F. Nielsen,, J. C. Frisvad, and, N. P. Keller. 2005. LaeA, a regulator of morphogenetic fungal virulence factors. Eukaryot. Cell 4:15741582.
16. Bok, J. W.,, D. Chung,, S. A. Balajee,, K. A. Marr,, D. Andes,, K. F. Nielsen,, J. C. Frisvad,, K. A. Kirby, and, N. P. Keller. 2006. GliZ, a transcriptional regulator of gliotoxin biosynthesis, contributes to Aspergillus fumigatus virulence. Infect. Immun. 74:67616768.
17. Bok, J. W., and, N. P. Keller. 2004. LaeA, a regulator of secondary metabolism in Aspergillus spp. Eukaryot. Cell 3:527535.
18. Bonnett, C. R.,, E. J. Cornish,, A. G. Harmsen, and, J. B. Burritt. 2006. Early neutrophil recruitment and aggregation in the murine lung inhibit germination of Aspergillus fumigatus conidia. Infect. Immun. 74:65286539.
19. Boudewijns, M.,, P. E. Verweij, and, W. J. Melchers. 2006. Molecular diagnosis of invasive aspergillosis: the long and winding road. Future Microbiol. 1:283293.
20. Bretz, C.,, G. Gersuk,, S. Knoblaugh,, N. Chaudhary,, J. Randolph-Habecker,, R. C. Hackman,, J. Staab, and, K. A. Marr. 2008. MyD88 signaling contributes to early pulmonary responses to Aspergillus fumigatus. Infect. Immun. 76:952958.
21. Bromley, M.,, C. Gordon,, N. Rovira-Graells, and, J. Oliver. 2006. The Aspergillus fumigatus cellobiohydrolase B (cbhB) promoter is tightly regulated and can be exploited for controlled protein expression and RNAi. FEMS Microbiol. Lett. 264:246254.
22. Brown, J. S.,, A. Aufauvre-Brown,, J. Brown,, J. M. Jennings,, H. Arst, Jr., and, D. W. Holden. 2000. Signature-tagged and directed mutagenesis identify PABA synthetase as essential for Aspergillus fumigatus pathogenicity. Mol. Micro-biol. 36:13711380.
23. Bruneau, J. M.,, T. Magnin,, E. Tagat,, R. Legrand,, M. Bernard,, M. Diaquin,, C. Fudali, and, J. P. Latge. 2001. Proteome analysis of Aspergillus fumigatus identifies glycosylphosphatidylinositol-anchored proteins associated to the cell wall biosynthesis. Electrophoresis 22:28122823.
24. Burgos, A.,, T. Zaoutis,, C. Dvorak,, J. Hoffman,, K. Knapp,, J. Nania,, P. Prasad, and, W. Steinbach. 2008. Pediatric invasive aspergillosis: a multiyear retrospective analysis of 139 contemporary cases. Pediatrics 121:e1286e1294.
25. Cagatay, A. A.,, F. Cosnan,, A. Karadeniz,, S. K. Besisik,, H. Ozsut,, M. Nalacaci,, Y. Pekcelen,, H. Eraksoy,, G. Dincol, and, S. Calangu. 2008. The clinical and pharmacoeconomic analysis of invasive aspergillosis in adult patients with haematological diseases. Mycoses 51:328335.
26. Cai, J. J.,, P. C. Woo,, S. K. Lau,, D. K. Smith, and, K. Y. Yuen. 2006. Accelerated evolutionary rate may be responsible for the emergence of lineage-specific genes in ascomycota. J. Mol. Evol. 63:111.
27. Calera, J. A.,, S. Paris,, M. Monod,, A. J. Hamilton,, J.-P. Debeaupuis,, M. Diaquin,, R. Lopez-Medrano,, F. Leal, and, J.-P. Latge. 1997. Cloning and disruption of the antigenic catalase gene of Aspergillus fumigatus. Infect. Immun. 65:47184724.
28. Casadevall, A., and, L. Pirofski. 1999. Host-pathogen interactions: redefining the basic concepts of virulence and pathogenicity. Infect. Immun. 67:37033713.
29. Casadevall, A., and, L. Pirofski. 2001. Host-pathogen interactions: the attributes of virulence. J. Infect. Dis. 184:337344.
30. Casadevall, A., and, L. Pirofski. 2003. The damage-response framework of microbial pathogenesis. Nat. Rev. Microbiol. 1:1724.
31. Chang, Y. C.,, H. F. Tsai,, M. Karos, and, K. J. Kwon-Chung. 2004. THTA, a thermotolerance gene of Aspergillus fumigatus. Fungal Genet. Biol. 41:888896.
32. Chauhan, N.,, J.-P. Latge, and, R. Calderone. 2006. Signalling and oxidant adaptation in Candida albicans and Aspergillus fumigatus. Nat. Rev. Microbiol. 4:435444.
33. Clemons, K. V.,, T. K. Miller,, C. P. Selitrennikoff, and, D. A. Stevens. 2002. fos-1, a putative histidine kinase as a virulence factor for systemic aspergillosis. Med. Mycol. 40:259262.
34. Cornillet, A.,, C. Camus,, S. Nimubona,, V. Gandemer,, P. Tattevin,, C. Belleguic,, S. Chevrier,, C. Meuiner,, C. Lebert,, M. Aupee,, S. Caulet-Maugendre,, M. Faucheux,, B. Lelong,, E. Leray,, C. Guiguen, and, J.-P. Gangneux. 2006. Comparison of epidemiological, clinical, and biological features of invasive aspergillosis in neutropenic and nonneutropenic patients: a 6-year survey. Clin. Infect. Dis. 43:577584.
35. Coyle, C. M.,, S. C. Kenaley,, W. R. Rittenour, and, D. G. Panaccione. 2007. Association of ergot alkaloids with conidiation in Aspergillus fumigatus. Mycologia 99:804811.
36. Cramer, R. A., Jr.,, M. P. Gamcsik,, R. M. Brooking,, L. K. Najvar,, W. R. Kirkpatrick,, T. F. Patterson,, C. J. Balibar,, J. R. Graybill,, J. R. Perfect,, S. N. Abraham, and, W. J. Steinbach. 2006. Disruption of a nonribosomal peptide synthetase in Aspergillus fumigatus eliminates gliotoxin production. Eukaryot. Cell 5:972980.
37. Cramer, R. A., Jr.,, B. Z. Perfect,, N. Pinchai,, S. Park,, D. S. Perlin,, Y. G. Asfaw,, J. Heitman,, J. R. Perfect, and, W. J. Steinbach. 2008. Calcineurin target CrzA regulates conidial germination, hyphal growth and pathogenesis of Aspergillus fumigatus. Eukaryot. Cell 7:10851097.
38. Dagenais, T. R.,, D. Chung,, S. S. Giles,, C. M. Hull,, D. Andes, and, N. P. Keller. 2008. Defects in conidiophore development and conidial/macrophage interactions in a dioxygenase mutant of Aspergillus fumigatus. Infect Immun. 76:32143220.
39. da Silva Ferreira, M. E.,, T. Heinekamp,, A. Hartl,, A. A. Brakhage,, C. P. Semighini,, S. D. Harris,, M. Savoldi,, P. F. de Gouvea,, M. H. de Souza Goldman, and, G. H. Goldman. 2007. Functional characterization of the Aspergillus fumigatus calcineurin. Fungal Genet. Biol. 44:219230.
40. da Silva Ferreira, M. E.,, M. R. Kress,, M. Savoldi,, M. H. Goldman,, A. Hartl,, T. Heinekamp,, A. A. Brakhage, and, G. H. Goldman. 2006a. The akuB(KU80) mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus. Eukaryot. Cell 5:207211.
41. da Silva Ferreira, M. E.,, I. Malavazi,, M. Savoldi,, A. A. Brakhage,, M. H. Goldman,, H. S. Kim,, W. C. Nierman, and, G. H. Goldman. 2006b. Transcriptome analysis of Aspergillus fumigatus exposed to voriconazole. Curr. Genet. 50:3244.
42. De Backer, M. D.,, T. Ilyina,, X. J. Ma,, S. Vandoninck,, W. H. Luyten, and, H. Vanden Bossche. 2001. Genomic profiling of the response of Candida albicans to itraconazole treatment using a DNA microarray. Antimicrob. Agents Chemother. 45:16601670.
43. d’Enfert, C.,, M. Diaquin,, A. Delit,, N. Wuscher,, J. Debeaupuis,, M. Huerre, and, J. Latge. 1996. Attenuated virulence of uridine-uracil auxotrophs of Aspergillus fumigatus. Infect. Immun. 64:44014405.
44. Du, C.,, J. Sarfati,, J. P. Latge, and, R. Calderone. 2006. The role of the sakA (Hog1) and tcsB (sln1) genes in the oxidant adaptation of Aspergillus fumigatus. Med. Mycol. 44:211218.
45. Eisenberg, T.,, S. Buttner,, G. Kroemer, and, F. Madeo. 2007. The mitochondrial pathway in yeast apoptosis. Apoptosis 12:10111023.
46. Eisenstein, D. J.,, P. W. Biddinger, and, J. C. Rhodes. 1990. Experimental murine invasive pulmonary aspergillosis. Am. J. Clin. Pathol. 93:510515.
47. Epstein, S. M.,, E. Verney,, T. M. Miale, and, H. Sidransky. 1967. Studies on the pathogenesis of experimental pulmonary aspergillosis. Am. J. Pathol. 51:769788.
48. Fedorova, N. D.,, J. H. Badger,, G. D. Robson,, J. R. Wortman, and, W. C. Nierman. 2005. Comparative analysis of programmed cell death pathways in filamentous fungi. BMC Genomics 6:177.
49. Fedorova, N. D.,, N. Khaldi,, V. S. Joardar,, R. Maiti,, P. Amedeo,, M. J. Anderson,, J. Crabtree,, J. C. Silva,, J. H. Badger,, A. Albarraq,, S. Angiuoli,, H. Bussey,, P. Bowyer,, P. J. Cotty,, P. S. Dyer,, A. Egan,, K. Galens,, C. M. Fraser-Liggett,, B. J. Haas,, J. M. Inman,, R. Kent,, S. Lemieux,, I. Malavazi,, J. Orvis,, T. Roemer,, C. M. Ronning,, J. P. Sundaram,, G. Sutton,, G. Turner,, J. C. Venter,, O. R. White,, B. R. Whitty,, P. Youngman,, K. H. Wolfe,, G. H. Goldman,, J. R. Wortman,, B. Jiang,, D. W. Denning, and, W. C. Nierman. 2008. Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus. PLoS Genet. 4:e1000046.
50. Ferreira, M. E.,, A. L. Colombo,, I. Paulsen,, Q. Ren,, J. Wortman,, J. Huang,, M. H. Goldman, and, G. H. Goldman. 2005. The ergosterol biosynthesis pathway, transporter genes, and azole resistance in Aspergillus fumigatus. Med. Mycol. 43 (Suppl. 1):S313S319.
51. Fortwendel, J. R.,, J. C. Panepinto,, A. E. Seitz,, D. S. Askew, and, J. C. Rhodes. 2004. Aspergillus fumigatus rasA and rasB regulate the timing and morphology of asexual development. Fungal Genet. Biol. 41:129139.
52. Fortwendel, J. R.,, W. Zhao,, R. Bhabhra,, S. Park,, D. S. Perlin,, D. S. Askew, and, J. C. Rhodes. 2005. A fungus-specific ras homolog contributes to the hyphal growth and virulence of Aspergillus fumigatus. Eukaryot. Cell 4:19821989.
53. Galagan, J. E.,, S. E. Calvo,, C. Cuomo,, L. J. Ma,, J. R. Wortman,, S. Batzoglou,, S. I. Lee,, M. Basturkmen,, C. C. Spevak,, J. Clutterbuck,, V. Kapitonov,, J. Jurka,, C. Scazzocchio,, M. Farman,, J. Butler,, S. Purcell,, S. Harris,, G. H. Braus,, O. Draht,, S. Busch,, C. D’Enfert,, C. Bouchier,, G. H. Goldman,, D. Bell-Pedersen,, S. Griffiths-Jones,, J. H. Doonan,, J. Yu,, K. Vienken,, A. Pain,, M. Freitag,, E. U. Selker,, D. B. Archer,, M. A. Penalva,, B. R. Oakley,, M. Momany,, T. Tanaka,, T. Kumagai,, K. Asai,, M. Machida,, W. C. Nierman,, D. W. Denning,, M. Caddick,, M. Hynes,, M. Paoletti,, R. Fischer,, B. Miller,, P. Dyer,, M. S. Sachs,, S. A. Osmani, and, B. W. Birren. 2005. Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature 438:11051115.
54. Gardiner, D. M.,, P. Waring, and, B. J. Howlett. 2005. The epipolythiodioxopiperazine (ETP) class of fungal toxins: distribution, mode of action, functions and biosynthesis. Microiology 151:10211032.
55. Garrido, C., and, G. Kroemer. 2004. Life’s smile, death’s grin: vital functions of apoptosis-executing proteins. Curr. Opin. Cell Biol. 16:639646.
56. Gautam, P.,, C. S. Sundaram,, T. Madan,, W. N. Gade,, A. Shah,, R. Sirdeshmukh, and, P. U. Sarma. 2007. Identification of novel allergens of Aspergillus fumigatus using immunoproteomics approach. Clin. Exp. Allergy 37:12391249.
57. Gibson, P. G. 2006. Allergic bronchopulmonary aspergillosis. Semin. Respir. Crit. Care Med. 27:185191.
58. Glass, N. L., and, K. Dementhon. 2006. Non-self recognition and programmed cell death in filamentous fungi. Curr. Opin. Microbiol. 9:553558.
59. Glimp, R., and, A. S. Bayer. 1981. Fungal pneumonias. Part 3. Allergic bronchopulmonary aspergillosis. Chest 80:8594.
60. Graybill, J. R.,, S. R. Kaster, and, D. J. Drutz. 1983. Treatment of experimental murine aspergillosis with BAY n7133. J. Infect. Dis. 148:898906.
61. Grosse, C.,, T. Heinekamp,, O. Kniemeyer,, A. Gehrke, and, A. A. Brakhage. 2008. Protein kinase A regulates growth, sporulation and pigment formation in Aspergillus fumigatus. Appl Environ Microbiol. 74:49234933.
62. Henry, C.,, I. Mouyna, and, J. P. Latge. 2007. Testing the efficacy of RNA interference constructs in Aspergillus fumigatus. Curr. Genet. 51:277284.
63. Hensel, M.,, H. N. Arst, Jr.,, A. Aufauvre-Brown, and, D. W. Holden. 1998. The role of the Aspergillus fumigatus areA gene in invasive pulmonary aspergillosis. Mol. Gen. Genet. 258:553557.
64. Hissen, A. H.,, A. N. Wan,, M. L. Warwas,, L. J. Pinto, and, M. M. Moore. 2005. The Aspergillus fumigatus siderophore biosynthetic gene sidA, encoding L-ornithine N5-oxygenase, is required for virulence. Infect. Immun. 73:54935503.
65. Hoffmann, B.,, O. Valerius,, M. Andermann, and, G. H. Braus. 2001. Transcriptional autoregulation and inhibition of mRNA translation of amino acid regulator gene cpcA of filamentous fungus Aspergillus nidulans. Mol. Biol. Cell 12:28462857.
66. Hope, W.,, M. Kruhlak,, C. Lyman,, R. Petraitiene,, C. Petraitis,, A. Francesconi,, M. Kasai,, D. Mickiene,, T. Sein,, J. Peter,, A. Kelaher,, J. Hughes,, M. Cotton,, C. Cotten,, J. Bacher,, S. Tripathi,, L. Bermudez,, T. Maugel,, P. Zerfas,, J. Wingard,, G. Drusano, and, T. Walsh. 2007. Pathogenesis of Aspergillus fumigatus and the kinetics of galactomannan in an in vitro model of early invasive pulmonary aspergillosis: implications for antifungal therapy. J. Infect. Dis. 195:455466.
67. Hospenthal, D. R.,, K. J. Kwon-Chung, and, J. E. Bennett. 1998. Concentrations of airborne Aspergillus compared to the incidence of invasive aspergillosis: lack of correlation. Med. Mycol. 36:165168.
68. Hu, W.,, S. Sillaots,, S. Lemieux,, J. Davison,, S. Kauffman,, A. Breton,, A. Linteau,, C. Xin,, J. Bowman,, J. Becker,, B. Jiang, and, T. Roemer. 2007. Essential gene identification and drug target prioritization in Aspergillus fumigatus. PLoS Pathog. 3:e24.
69. Ibrahim-Granet, O.,, M. Dubourdeau,, J. P. Latge,, P. Ave,, M. Huerre,, A. A. Brakhage, and, M. Brock. 2008. Methylcitrate synthase from Aspergillus fumigatus is essential for manifestation of invasive aspergillosis. Cell. Microbiol. 10:134148.
70. Ibrahim-Granet, O.,, B. Philippe,, H. Boleti,, E. Boisvieux-Ulrich,, D. Grenet,, M. Stern, and, J. P. Latge. 2003. Phagocytosis and intracellular fate of Aspergillus fumigatus conidia in alveolar macrophages. Infect. Immun. 71:891903.
71. Jadoun, J.,, Y. Shadkchan, and, N. Osherov. 2004. Disruption of the Aspergillus fumigatus argB gene using a novel in vitro transposon-based mutagenesis approach. Curr. Genet. 45:235241.
72. Jaques, A. K.,, T. Fukamizo,, D. Hall,, R. C. Barton,, G. M. Escott,, T. Parkinson,, C. A. Hitchcock, and, D. J. Adams. 2003. Disruption of the gene encoding the ChiB1 chitinase of Aspergillus fumigatus and characterization of a recombinant gene product. Microbiology 149:29312939.
73. Jaton-Ogay, K.,, S. Paris,, M. Huerre,, M. Quadroni,, R. Falchetto,, G. Togni,, J.-P. Latge, and, M. Monod. 1994. Cloning and disruption of the gene encoding an extracellular metalloprotease of Aspergillus fumigatus. Mol. Microbiol. 14:917928.
74. Keller, N. P.,, G. Turner, and, J. W. Bennett. 2005. Fungal secondary metabolism—from biochemistry to genomics. Nat. Rev. Microbiol. 3:937947.
75. Khalaj, V.,, H. Eslami,, M. Azizi,, N. Rovira-Graells, and, M. Bromley. 2007. Efficient downregulation of alb1 gene using an AMA1-based episomal expression of RNAi construct in Aspergillus fumigatus. FEMS Microbiol. Lett. 270:250254.
76. Kim, K. H.,, K. M. Brown,, P. V. Harris,, J. A. Langston, and, J. R. Cherry. 2007. A proteomics strategy to discover beta-glucosidases from Aspergillus fumigatus with two-dimensional page in-gel activity assay and tandem mass spectrometry. J. Proteome Res. 6:47494757.
77. Kniemeyer, O.,, F. Lessing,, O. Scheibner,, C. Hertweck, and, A. A. Brakhage. 2006. Optimisation of a 2-D gel electrophoresis protocol for the human-pathogenic fungus Aspergillus fumigatus. Curr. Genet. 49:178189.
78. Kontoyiannis, D. P., and, G. P. Bodey. 2002. Invasive aspergillosis in 2002: an update. Eur. J. Clin. Microbiol. Infect. Dis. 21:161172.
79. Kragl, C.,, M. Schrettl,, B. Abt,, B. Sarg,, H. H. Lindner, and, H. Haas. 2007. EstB-mediated hydrolysis of the siderophore triacetylfusarinine C optimizes iron uptake of Aspergillus fumigatus. Eukaryot. Cell 6:12781285.
80. Krappmann, S. 2006. Tools to study molecular mechanisms of Aspergillus pathogenicity. Trends Microbiol. 14:354364.
81. Krappmann, S.,, O. Bayram, and, G. H. Braus. 2005. Deletion and allelic exchange of the Aspergillus fumigatus veA locus via a novel recyclable marker module. Eukaryot. Cell 4:12981307.
82. Krappmann, S.,, E. M. Bignell,, U. Reichard,, T. Rogers,, K. Haynes, and, G. H. Braus. 2004. The Aspergillus fumigatus transcriptional activator CpcA contributes significantly to the virulence of this fungal pathogen. Mol. Microbiol. 52:785799.
83. Krappmann, S.,, C. Sasse, and, G. Braus. 2006. Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end-joining-deficient genetic background. Eukaryot. Cell 5:212215.
84. Kupfahl, C.,, T. Heinekamp,, G. Geginat,, T. Ruppert,, A. Hartl,, H. Hof, and, A. A. Brakhage. 2006. Deletion of the gliP gene of Aspergillus fumigatus results in loss of gliotoxin production but has no effect on virulence of the fungus in a low-dose mouse infection model. Mol. Microbiol. 62:292302.
85. Kurup, V. P.,, A. P. Knutsen,, R. B. Moss, and, N. K. Bansal. 2006. Specific antibodies to recombinant allergens of Aspergillus fumigatus in cystic fibrosis patients with ABPA. Clin. Mol. Allergy 4:11.
86. Lamarre, C.,, O. Ibrahim-Granet,, C. Du,, R. Calderone, and, J. P. Latge. 2007. Characterization of the SKN7 ortholog of Aspergillus fumigatus. Fungal Genet. Biol. 44:682690.
87. Langfelder, K.,, S. Gattung, and, A. A. Brakhage. 2002. A novel method used to delete a new Aspergillus fumigatus ABC transporter-encoding gene. Curr. Genet. 41:268274.
88. Langfelder, K.,, B. Jahn,, H. Gehringer,, A. Schmidt,, G. Wanner, and, A. A. Brakhage. 1998. Identification of a polyke-tide synthase gene (pksP) of Aspergillus fumigatus involved in conidial pigment biosynthesis and virulence. Med. Microbiol. Immunol. 187:7989.
89. Latge, J.-P. 1999. Aspergillus fumigatus and aspergillosis. Clin. Microbiol. Rev. 12:310350.
90. Lessing, F.,, O. Kniemeyer,, I. Wozniok,, J. Loeffler,, O. Kurzai,, A. Haertl, and, A. A. Brakhage. 2007. The Aspergillus fumigatus transcriptional regulator AfYap1 represents the major regulator for defense against reactive oxygen intermediates but is dispensable for pathogenicity in an intranasal mouse infection model. Eukaryot. Cell 6:22902302.
91. Lewis, R. E.,, N. P. Wiederhold,, J. Chi,, X. Y. Han,, K. V. Komanduri,, D. P. Kontoyiannis, and, R. A. Prince. 2005a. Detection of gliotoxin in experimental and human aspergillosis. Infect. Immun. 73:635637.
92. Lewis, R. E.,, N. P. Wiederhold,, M. S. Lionakis,, R. A. Prince, and, D. P. Kontoyiannis. 2005b. Frequency and species distribution of gliotoxin-producing Aspergillus isolates recovered from patients at a tertiary-care cancer center. J. Clin. Microbiol. 43:61206122.
93. Li, H.,, H. Zhou,, Y. Luo,, H. Ouyang,, K. Hu, and, C. Jin. 2007. Glycosylphosphatidylinositol (GPI) anchor is required in Aspergillus fumigatus for morphogenesis and virulence. Mol. Microbiol. 64:10141027.
94. Liebmann, B.,, S. Gattung,, B. Jahn, and, A. A. Brakhage. 2003. cAMP signaling in Aspergillus fumigatus is involved in the regulation of the virulence gene pksP and in defense against macrophage killing by macrophages. Mol. Genet. Genomics 269:420435.
95. Liebmann, B.,, T. W. Muhleisen,, M. Muller,, M. Hecht,, G. Weidner,, A. Braun,, M. Brock, and, A. A. Brakhage. 2004a. Deletion of the Aspergillus fumigatus lysine biosynthesis gene lysF encoding homoaconitase leads to attenuated virulence in a low-dose model mouse infection model of invasive aspergillosis. Arch. Microbiol. 181:378383.
96. Liebmann, B.,, M. Muller,, A. Braun, and, A. A. Brakhage. 2004b. The cyclic AMP-dependent protein kinase A network regulates development and virulence in Aspergillus fumigatus. Infect. Immun. 72:51935203.
97. Lionakis, M. S.,, R. E. Lewis,, G. S. May,, N. P. Wiederhold,, N. D. Albert,, G. Halder, and, D. P. Kontoyiannis. 2005. Toll-deficient Drosophila flies as a fast, high-throughput model for the study of antifungal drug efficacy against aspergillosis and Aspergillus virulence. J. Infect. Dis. 191:11881195.
98. Ma, Y.,, J. Qiao,, W. Liu,, Z. Wan,, X. Wang,, R. Calderone, and, R. Li. 2008. The Sho1 sensor regulates growth, morphology, and oxidant adaptation in Aspergillus fumigatus but is not essential for development of invasive pulmonary aspergillosis. Infect. Immun. 76:16951701.
99. Machida, M.,, K. Asai,, M. Sano,, T. Tanaka,, T. Kumagai,, G. Terai,, K. Kusumoto,, T. Arima,, O. Akita,, Y. Kashiwagi,, K. Abe,, K. Gomi,, H. Horiuchi,, K. Kitamoto,, T. Kobayashi,, M. Takeuchi,, D. W. Denning,, J. E. Galagan,, W. C. Nierman,, J. Yu,, D. B. Archer,, J. W. Bennett,, D. Bhatnagar,, T. E. Cleveland,, N. D. Fedorova,, O. Gotoh,, H. Horikawa,, A. Hosoyama,, M. Ichinomiya,, R. Igarashi,, K. Iwashita,, P. R. Juvvadi,, M. Kato,, Y. Kato,, T. Kin,, A. Kokubun,, H. Maeda,, N. Maeyama,, J. Maruyama,, H. Nagasaki,, T. Nakajima,, K. Oda,, K. Okada,, I. Paulsen,, K. Sakamoto,, T. Sawano,, M. Takahashi,, K. Takase,, Y. Terabayashi,, J. R. Wortman,, O. Yamada,, Y. Yamagata,, H. Anazawa,, Y. Hata,, Y. Koide,, T. Komori,, Y. Koyama,, T. Minetoki,, S. Suharnan,, A. Tanaka,, K. Isono,, S. Kuhara,, N. Ogasawara, and, H. Kikuchi. 2005. Genome sequencing and analysis of Aspergillus oryzae. Nature 438:11571161.
100. Mackenzie, D. W. R. 1988. Aspergillus in Man, p.1–8. In H. VandenBossche, D. W. R. Mackenzie, and G. Cauwenbergh (ed.), Aspergillus and Aspergillosis. Plenum Press, New York, NY.
101. Maerker, C.,, M. Rohde,, A. A. Brakhage, and, M. Brock. 2005. Methylcitrate synthase from Aspergillus fumigatus. Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia. FEBS J. 272:36153630.
102. Mah, J. H., and, J. H. Yu. 2006. Upstream and downstream regulation of asexual development in Aspergillus fumigatus. Eukaryot. Cell 5:15851595.
103. Marr, K. A.,, T. Patterson, and, D. Denning. 2002. Aspergillosis. Pathogenesis, clinical manifestations, and therapy. Infect. Dis. Clin. N. Am. 16:875894.
104. Maubon, D.,, S. Park,, M. Tanguy,, M. Huerre,, C. Schmitt,, M. C. Prevost,, D. S. Perlin,, J. P. Latge, and, A. Beauvais. 2006. AGS3, an alpha(1-3) glucan synthase gene family member of Aspergillus fumigatus, modulate mycelium growth in the lung of experimentally infected mice. Fungal Genet. Biol. 43:366375.
105. McCarthy, J. E. 1998. Posttranscriptional control of gene expression in yeast. Microbiol. Mol. Biol. Rev. 62:14921553.
106. Meersseman, W.,, L. Lagrou,, J. Maertens, and, E. VanWijngaerden. 2007. Invasive aspergillosis in the intensive care unit. Clin. Infect. Dis. 45:205216.
107. Mehrad, B.,, R. M. Strieter,, T. A. Moore,, W. C. Tsai,, S. A. Lira, and, T. J. Standiford. 1999. CXC chemokine receptor-2 ligands are necessary components of neutrophil-mediated host defense in invasive pulmonary aspergillosis. J. Immunol. 163:60866094.
108. Mellado, E.,, A. Aufauvre-Brown,, N. A. Gow, and, D. W. Holden. 1996a. The Aspergillus fumigatus chsC and chsG genes encode class III chitin synthases with different functions. Mol. Microbiol. 20:667679.
109. Mellado, E.,, G. Garcia-Effron,, M. J. Buitrago,, L. Alcazar-Fuoli,, M. Cuenca-Estrella, and, J. L. Rodriguez-Tudela. 2005. Targeted gene disruption of the 14-alpha sterol demethylase (cyp51A) in Aspergillus fumigatus and its role in azole drug susceptibility. Antimicrob. Agents Chemother. 49:25362538.
110. Mellado, E.,, C. A. Specht,, P. W. Robbins, and, D. W. Holden. 1996b. Cloning and characterization of chsD, a chitin synthase-like gene of Aspergillus fumigatus. FEMS Microbiol. Lett. 143:6976.
111. Mennick-Kersten, M.,, D. Ruegebrink,, R. Klont,, A. Warris,, N. Blijlevens,, J. Donnelly, and, P. Verweij. 2008. Improved detection of circulating Aspergillus antigen by use of a modified pretreatment protocol. J. Clin. Microbiol. 46:13911397.
112. Miceli, M.,, M. Grazziutti,, G. Woods,, W. Zhao,, M. Kocoglu,, B. Barlogie, and, E. Anaissie. 2008. Strong correlation between serum Aspergillus galactomannan index and outcome of aspergillosis in patient with hematological cancer: clinical and research implications. Clin. Infect. Dis. 46:14121422.
113. Monod, M.,, S. Paris,, J. Sarfati,, K. Jaton-Ogay,, P. Ave, and, J.-P. Latge. 1993. Virulence of alkaline protease-deficient mutants of Aspergillus fumigatus. FEMS Microbiol. Lett. 106:3946.
114. Montagnoli, C.,, F. Fallarino,, R. Gaziano,, S. Bozza,, S. Bellocchio,, T. Zelante,, W. P. Kurup,, L. Pitzurra,, P. Puccetti, and, L. Romani. 2006. Immunity and tolerance to Aspergillus involve functionally distinct regulatory T cells and tryptophan metabolism. J. Immunol. 176:17121723.
115. Moreno, M. A.,, O. Ibrahim-Granet,, R. Vicentefranqueira,, J. Amich,, P. Ave,, F. Leal,, J. P. Latge, and, J. A. Calera. 2007. The regulation of zinc homeostasis by the ZafA transcriptional activator is essential for Aspergillus fumigatus virulence. Mol. Microbiol. 64:11821197.
116. Morgenstern, D. E.,, M. A. C. Gifford,, L. L. Li,, C. M. Doer-schuk, and, M. C. Dinauer. 1997. Absence of respiratory burst in X-linked chronic granulomatous disease mice leads to abnormalities in both host defense and inflammatory response to Aspergillus fumigatus. J. Exp. Med. 185:207218.
117. Mousavi, S. A., and, G. D. Robson. 2003. Entry into the stationary phase is associated with a rapid loss of viability and an apoptotic-like phenotype in the opportunistic pathogen Aspergillus fumigatus. Fungal Genet. Biol. 39:221229.
118. Mousavi, S. A., and, G. D. Robson. 2004. Oxidative and amphotericin B-mediated cell death in the opportunistic pathogen Aspergillus fumigatus is associated with an apoptotic-like phenotype. Microbiology 150:19371945.
119. Mouyna, I.,, C. Henry,, T. L. Doering, and, J. P. Latge. 2004. Gene silencing with RNA interference in the human pathogenic fungus Aspergillus fumigatus. FEMS Microbiol. Lett. 237:317324.
120. Mouyna, I.,, W. Morelle,, M. Vai,, M. Monod,, B. Lechenne,, T. Fontaine,, A. Beauvais,, J. Sarfati,, M. C. Prevost,, C. Henry, and, J. P. Latge. 2005. Deletion of GEL2 encoding for a beta(1-3) glucanosyltransferase affects morphogenesis and virulence in Aspergillus fumigatus. Mol. Microbiol. 56:16751688.
121. Mouyna., I.,, J. Sarfati,, P. Recco,, T. Fontaine,, B. Henrissatz, and, J. P. Latge. 2002. Molecular characterization of a cell wall-associated beta(1-3)endoglucanase of Aspergillus fumigatus. Med. Mycol. 40:455464.
122. Munoz, P.,, J. Guinea, and, E. Bouza. 2006. Update on invasive aspergillosis: clinical and diagnostic aspects. Clin. Microbiol. Infect. 12 (Suppl. 7):2439.
123. Mylonakis, E.,, A. Casadevall, and, F. M. Ausubel. 2007. Exploiting amoeboid and non-vertebrate animal model systems to study the virulence of human pathogenic fungi. PLoS Pathog. 3:e101.
124. Neafsey, D. E., and, J. E. Galagan. 2007. Dual modes of natural selection on upstream open reading frames. Mol. Biol. Evol. 24:17441751.
125. Nierman, W. C.,, A. Pain,, M. J. Anderson,, J. R. Wortman,, H. S. Kim,, J. Arroyo,, M. Berriman,, K. Abe,, D. B. Archer,, C. Bermejo,, J. Bennett,, P. Bowyer,, D. Chen,, M. Collins,, R. Coulsen,, R. Davies,, P. S. Dyer,, M. Farman,, N. Fedorova,, T. V. Feldblyum,, R. Fischer,, N. Fosker,, A. Fraser,, J. L. Garcia,, M. J. Garcia,, A. Goble,, G. H. Goldman,, K. Gomi,, S. Griffith-Jones,, R. Gwilliam,, B. Haas,, H. Haas,, D. Harris,, H. Horiuchi,, J. Huang,, S. Humphray,, J. Jimenez,, N. Keller,, H. Khouri,, K. Kitamoto,, T. Kobayashi,, S. Konzack,, R. Kulkarni,, T. Kumagai,, A. Lafon,, J. P. Latge,, W. Li,, A. Lord,, C. Lu,, W. H. Majoros,, G. S. May,, B. L. Miller,, Y. Mohamoud,, M. Molina,, M. Monod,, I. Mouyna,, S. Mulligan,, L. Murphy,, S. O’Neil,, I. Paulsen,, M. A. Penalva,, M. Pertea,, C. Price,, B. L. Pritchard,, M. A. Quail,, E. Rabbinowitsch,, N. Rawlins,, M. A. Rajandream,, U. Reichard,, H. Renauld,, G. D. Robson,, S. Rodriguez de Cordoba,, J. M. Rodriguez-Pena,, C. M. Ronning,, S. Rutter,, S. L. Salzberg,, M. Sanchez,, J. C. Sanchez-Ferrero,, D. Saunders,, K. Seeger,, R. Squares,, S. Squares,, M. Takeuchi,, F. Tekaia,, G. Turner,, C. R. Vazquez de Aldana,, J. Weidman,, O. White,, J. Woodward,, J. H. Yu,, C. Fraser,, J. E. Galagan,, K. Asai,, M. Machida,, N. Hall,, B. Barrell, and, D. W. Denning. 2005. Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. Nature 438:11511156.
126. Olivas, I.,, M. Royuela,, B. Romero,, M. C. Monteiro,, J. M. Minguez,, F. Laborda, and, J. R. Lucas. 2008. Ability to grow on lipids accounts for the fully virulent phenotype in neutropenic mice of Aspergillus fumigatus null mutants in the key glyoxylate cycle enzymes. Fungal Genet. Biol. 45:4560.
127. Panepinto, J. C.,, B. G. Oliver,, J. R. Fortwendel,, D. L. Smith,, D. S. Askew, and, J. C. Rhodes. 2003. Deletion of the Aspergillus fumigatus gene encoding the Ras-related protein RhbA reduces virulence in a model of Invasive pulmonary aspergillosis. Infect. Immun. 71:28192826.
128. Paoletti, M.,, C. Rydholm,, E. U. Schwier,, M. J. Anderson,, G. Szakacs,, F. Lutzoni,, J. P. Debeaupuis,, J. P. Latge,, D. W. Denning, and, P. S. Dyer. 2005. Evidence for sexuality in the opportunistic fungal pathogen Aspergillus fumigatus. Curr. Biol. 15:12421248.
129. Paris, S.,, J. P. Debeaupuis,, R. Crameri,, M. Carey,, F. Charles,, M. C. Prevost,, C. Schmitt,, B. Philippe, and, J. P. Latge. 2003a. Conidial hydrophobins of Aspergillus fumigatus. Appl. Environ. Microbiol. 69:15811588.
130. Paris, S.,, M. Monod,, M. Diaquin,, B. Lamy,, L. K. Arruda,, P. J. Punt, and, J.-P. Latge. 1993. A transformant of Aspergillus fumigatus deficient in the antigenic cytotoxin ASPFI. FEMS Microbiol. Lett. 111:3136.
131. Paris, S.,, D. Wysong,, J.-P. Debeaupuis,, K. Shibuya,, B. Philippe,, R. D. Diamond, and, J.-P. Latge. 2003b. Catalases of Aspergillus fumigatus. Infect. Immun. 71:35513562.
132. Paul, A.,, S. Hackbarth,, R. D. Vogt,, B. Roder,, B. K. Burnison, and, C. E. Steinberg. 2004. Photogeneration of singlet oxygen by humic substances: comparison of humic substances of aquatic and terrestrial origin. Photochem. Photo-biol. Sci. 3:273280.
133. Penack, O.,, P. Rempf,, B. Graf,, E. Thiel, and, I. W. Blau. 2008. False-positive Aspergillus antigen testing due to application of piperacillin-tazobactam—is it still an issue? Diagn. Microbiol. Infect. Dis. 60:117120.
134. Perrin, R. M.,, N. D. Fedorova,, J. W. Bok,, R. A. Cramer,, J. R. Wortman,, H. S. Kim,, W. C. Nierman, and, N. P. Keller. 2007. Transcriptional regulation of chemical diversity in Aspergillus fumigatus by LaeA. PLoS Pathog. 3:e50.
135. Petraitis, V.,, R. Petraitiene,, J. Solomon,, A. H. Kelaher,, H. A. Murray,, C. Mya-Sun,, A. K. Bhandary,, T. Sien,, N. A. Avila,, A. Basevicius,, J. Bacher, and, T. J. Walsh. 2006. Multidimensional volumetric imaging of pulmonary infiltrates for measuring therapeutic response to antifungal therapy in experimental invasive pulmonary aspergillosis. Antimicrob. Agents Chemother. 50:15101517.
136. Pinan-Lucarre, B.,, M. Paoletti,, K. Dementhon,, B. Coulary-Salin, and, C. Clave. 2003. Autophagy is induced during cell death by incompatibility and is essential for differentiation in the filamentous fungus Podospora anserina. Mol. Microbiol. 47:321333.
137. Poggeler, S. 2002. Genomic evidence for mating abilities in the asexual pathogen Aspergillus fumigatus. Curr. Genet. 42:153160.
138. Pyrzak, W.,, K. Y. Miller, and, B. L. Miller. 2008. Mating type protein Mat1–2 from asexual Aspergillus fumigatus drives sexual reproduction in fertile Aspergillus nidulans. Eukaryot. Cell 7:10291040.
139. Ramsdale, M. 2008. Programmed cell death in pathogenic fungi. Biochim. Biophys. Acta 1783:13691380.
140. Reeves, E. P.,, K. Reiber,, C. Neville,, O. Scheibner,, K. Kavanaugh, and, S. Doyle. 2006. A nonribosomal peptide synthetase (Pes1) confers protection against oxidative stress in Aspergillus fumigatus. FEBS J. 273:30383053.
141. Reichard, U.,, G. T. Cole,, T. W. Hill,, R. Ruchel, and, M. Monod. 2000a. Molecular characterization and influence on fungal development of ALP2, a novel serine protease from Aspergillus fumigatus. Int. J. Med. Microbiol. 290:549558.
142. Reichard, U.,, G. T. Cole,, R. Ruchel, and, M. Monod. 2000b. Molecular cloning and targeted deletion of PEP2 which encodes a novel aspartic proteinase from Aspergillus fumigatus. Int. J. Med. Microbiol. 290:8596.
143. Reichard, U.,, M. Monod,, F. Odds, and, R. Ruchel. 1997. Virulence of an aspergillopepsin-deficient mutant of Aspergillus fumigatus and evidence for another aspartic proteinase linked to the fungal cell wall. J. Med. Vet. Mycol. 35:189196.
144. Renwick, J.,, P. Daly,, E. P. Reeves, and, K. Kavanaugh. 2006. Susceptibility of larvae of Galleria mellonella to infection by Aspergillus fumigatus is dependent upon stage of conidial germination. Mycopathologia 161:377384.
145. Reyes, G.,, A. Romans,, C. K. Nguyen, and, G. S. May. 2006. Novel mitogen-activated protein kinase MpkC of Aspergillus fumigatus is required for utilization of polyalcohol sugars. Eukaryot. Cell 5:19341940.
146. Richardson, M. D., and, M. Kokki. 2003. Aspergillus, p. 273–296. In E. J. Anaissie, M. R. McGinnis, and M. A. Pfaller. (ed.), Clinical Mycology. Churchill Livingstone, Philadelphia, PA.
147. Richie, D.,, K. Fuller,, J. Fortwendel,, M. Miley,, J. McCarthy,, M. Feldmesser,, J. Rhodes, and, D. Askew. 2007a. Unexpected link between metal ion deficiency and autophagy in Aspergillus fumigatus. Eukaryot. Cell 6:24372447.
148. Richie, D.,, M. Miley,, R. Bhabhra,, G. Robson,, J. Rhodes, and, D. Askew. 2007b. The Aspergillus fumigatus metacaspases CasA and CasB facilitate growth under conditions of endoplasmic reticulum stress. Mol. Microbiol. 63:591604.
149. Robson, G. D.,, J. Huang,, J. Wortman, and, D. B. Archer. 2005. A preliminary analysis of the process of protein secretion and the diversity of putative secreted hydrolases encoded in Aspergillus fumigatus: insights from the genome. Med. Mycol. 43 (Suppl. 1):S41S47.
150. Rohlfs, M.,, M. Albert,, N. P. Keller, and, F. Kempken. 2007. Secondary chemicals protect mould from fungivory. Biol. Lett. 3:523525.
151. Romano, J.,, G. Nimrod,, N. Bel-Tal,, Y. Shadkchan,, K. Baruch,, H. Sharon, and, N. Osherov. 2006. Disruption of the Aspergillus fumigatus ECM33 homologue results in rapid conidial germination, antifungal resistance and hypervirulence. Microbiology 152:19191928.
152. Romero, B.,, G. Turner,, I. Olivas,, F. Laborda, and, J. R. De Lucas. 2003. The Aspergillus nidulans alcA promoter drives tightly regulated conditional gene expression in Aspergillus fumigatus permitting validation of essential genes in this human pathogen. Fungal Genet. Biol. 40:103114.
153. Sandhu, D. K.,, R. S. Sandhu,, Z. U. Khan, and, V. N. Damodaran. 1976. Conditional virulence of a p-aminobenzoic acid requiring mutant of Aspergillus fumigatus. Infect. Immun. 13:527532.
154. Sarfati, J.,, M. Diaquin,, J. P. Debeaupuis,, A. Schmidt,, D. Lecaque,, A. Beauvais, and, J. P. Latge. 2002. A new experimental murine aspergillosis model to identify strains of Aspergillus fumigatus with reduced virulence. Jpn. J. Med. Mycol. 43:203213.
155. Sasse, C.,, E. M. Bignell,, M. Hasenberg,, K. Haynes,, M. Gunzer,, G. H. Braus, and, S. Krappmann. 2008. Basal expression of the Aspergillus fumigatus transcriptional activator CpcA is sufficient to support pulmonary aspergillosis. Fungal Genet. Biol. 45:693704.
156. Schaffner, A.,, H. Douglas, and, A. Braude. 1982. Selective protection against conidia by mononuclear and against mycelia by polymorphonuclear phagocytes in resistance to Aspergillus. J. Clin. Investig. 69:617631.
157. Schaffner, A., and, P. G. Frick. 1985. The effect of ketoconazole on amphotericin B in a model of disseminated aspergillosis. J. Infect. Dis. 151:902910.
158. Schmalhorst, P. S.,, S. Krappmann,, W. Vervecken,, M. Roede,, M. Muller,, G. Braus,, R. Contreras,, A. Braun,, H. Bakker, and, F. H. Routier. 2008. Contribution of galactofuranose to the virulence of the opportunistic pathogen Aspergillus fumigatus. Eukaryot. Cell 7:12681277.
159. Schmidt, A., and, D. I. Schmidt. 1999. J. B. Georg W. Fresenius and the description of the species Aspergillus fumigatus, p. 1–4. In A. A. Brakhage, B. Jahn, and A. Schmidt (ed.), Aspergillus fumigatus. Biology, Clinical Aspects and Molecular Approaches to Pathogenicity, vol. 2. Karger, Basel, Switzerland.
160. Schmitt, H. J.,, E. M. Bernard,, M. Hauser, and, D. Armstrong. 1988. Aerosol amphotericin B is effective for prophylaxis and therapy in a rat model of pulmonary aspergillosis. Antimicrob. Agents Chemother. 32:16761679.
161. Schobel, F.,, O. Ibrahim-Granet,, P. Ave,, J. P. Latge,, A. A. Brakhage, and, M. Brock. 2007. Aspergillus fumigatus does not require fatty acid metabolism via isocitrate lyase for development of invasive aspergillosis. Infect. Immun. 75:12371244.
162. Schrettl, M.,, E. Bignell,, C. Kragl,, C. Joechl,, T. Rogers,, H. N. Arst, Jr.,, K. Haynes, and, H. Haas. 2004. Siderophore biosynthesis but not reductive iron assimilation is essential for Aspergillus fumigatus virulence. J. Exp. Med. 200:12131219.
163. Schrettl, M.,, E. Bignell,, C. Kragl,, Y. Sabiha,, O. Loss,, M. Eisendle,, A. Wallner,, H. N. Arst, Jr.,, K. Haynes, and, H. Haas. 2007. Distinct roles for intra- and extracellular siderophores during Aspergillus fumigatus infection. PLoS Pathog. 3:11951207.
164. Schwerk, C., and, K. Schulze-Osthoff. 2003. Non-apoptotic functions of caspases in cellular proliferation and differentiation. Biochem. Pharmacol. 66:14531458.
165. Sheppard, D. C.,, T. Doedt,, L. Y. Chiang,, H. S. Kim,, D. Chen,, W. C. Nierman, and, S. G. Filler. 2005. The Aspergillus fumigatus StuA protein governs the up-regulation of a discrete transcriptional program during the acquisition of developmental competence. Mol. Biol. Cell 16:58665879.
166. Sheppard, D. C.,, J. R. Graybill,, L. K. Najvar,, L. Y. Chiang,, T. Doedt,, W. R. Kirkpatrick,, R. Bocanegra,, A. C. Vallor,, T. F. Patterson, and, S. G. Filler. 2006. Standardization of an experimental murine model of invasive pulmonary aspergillosis. Antimicrob. Agents Chemother. 50:35013503.
167. Sheppard, D. C.,, G. Rieg,, L. Y. Chiang,, S. G. Filler,, J. E. Edwards, Jr., and, A. S. Ibrahim. 2004. Novel inhalational murine model of invasive pulmonary aspergillosis. Antimicrob. Agents Chemother. 48:19081911.
168. Shibuya, K.,, M. Takaoka,, K. Uchida,, M. Wakayama,, H. Yamaguchi,, K. Takahashi,, S. Paris,, J.-P. Latge, and, S. Naoe. 1999. Histopathology of experimental invasive pulmonary aspergillosis in rats: pathological comparison of pulmonary lesions induced by specific virulent factor deficient mutants. Microb. Pathog. 27:123131.
169. Sidransky, H., and, L. Friedman. 1959. The effect of cortisone and antibiotic agents on experimental pulmonary aspergillosis. Am. J. Pathol. 35:169183.
170. Sidransky, H.,, E. Verney, and, H. Beede. 1965. Experimental pulmonary aspergillosis. Arch. Pathol. 79:299309.
171. Smith, J. M.,, J. E. Davies, and, D. W. Holden. 1993. Construction and pathogenicity of Aspergillus fumigatus mutants that do not produce the ribotoxin restrictocin. Mol. Micro-biol. 9:10711077.
172. Smith, J. M.,, C. M. Tang,, S. VanNoorden, and, D. W. Holden. 1994. Virulence of Aspergillus fumigatus double mutants lacking restrictocin and an alkaline protease in a low-dose model of invasive pulmonary aspergillosis. Infect. Immun. 62:52475254.
173. Soriani, F. M.,, I. Malavazi,, M. E. daSilva-Ferreira,, M. Savoldi,, M. R. VonZeska-Kress,, M. H. deSouza-Goldman,, O. Loss,, E. Bignell, and, G. H. Goldman. 2008. Functional characterization of the Aspergillus fumigatus CRZ1 homo-logue, CrzA. Mol. Microbiol. 67:12741291.
174. Soubani, A. O., and, P. H. Chandrasekar. 2002. The clinical spectrum of pulmonary aspergillosis. Chest 121:19881999.
175. Spikes, S.,, T. Xu,, C. K. Nguyen,, G. Chamilos,, D. P. Kontoyiannis,, R. H. Jacobson,, D. E. Ejzykowocz,, L. Y. Chiang,, S. G. Filler, and, G. S. May. 2008. Gliotoxin production in Aspergillus fumigatus contributes to host-specific differences in virulence. J. Infect. Dis. 197:479486.
176. Spreadbury, C. L.,, T. Krausz,, S. Pervez, and, J. Cohen. 1989. Invasive aspergillosis: clinical and pathological features of a new animal model. J. Med. Vet. Mycol. 27:515.
177. Steinbach, W. J.,, R. M. Addison,, L. McLaughlin,, Q. Gerrald,, P. L. Martin,, T. Driscoll,, C. Bentsen,, J. R. Perfect, and, B. D. Alexander. 2007. Prospective Aspergillus galactomannan antigen testing in pediatric hematopoietic stem cell transplant recipients. Pediatr. Infect. Dis. J. 26:558564.
178. Steinbach, W. J.,, D. K. Benjamin, Jr.,, S. A. Trasi,, J. L. Miller,, W. A. Schell,, A. K. Zaas,, W. M. Foster, and, J. R. Perfect. 2004. Value of an inhalational model of invasive aspergillosis. Med. Mycol. 42:417425.
179. Steinbach, W. J.,, R. A. Cramer, Jr.,, B. Z. Perfect,, Y. G. Asfaw,, T. C. Sauer,, L. K. Najvar,, W. R. Kirkpatrick,, T. F. Patterson,, D. K. Benjamin, Jr.,, J. Heitman, and, J. R. Perfect. 2006. Calcineurin controls growth, morphology, and pathogenicity in Aspergillus fumigatus. Eukaryot. Cell 5:10911103.
180. Stephens-Romero, S. D.,, A. J. Mednick, and, M. Feldmesser. 2005. The pathogenesis of fatal outcome in murine pulmonary aspergillosis depends on the neutrophil depletion strategy. Infect. Immun. 73:114125.
181. Sugareva, V.,, A. Hartl,, M. Brock,, K. Hubner,, M. Roede,, T. Heinekamp, and, A. A. Brakhage. 2006. Characterisation of the laccase-encoding gene abr2 of the dihydroxynaphthalene-like melanin gene cluster of Aspergillus fumigatus. Arch. Microbiol. 186:345355.
182. Sugui, J. A.,, J. Pardo,, Y. C. Chang,, A. Mullenbacher,, K. A. Zarember,, E. M. Galvez,, L. Brinster,, P. Zerfas,, J. I. Gallin,, M. M. Simon, and, K. J. Kwon-Chung. 2007a. Role of laeA in the regulation of alb1, gliP, conidial morphology, and virulence in Aspergillus fumigatus. Eukaryot. Cell 6:15521561.
183. Sugui, J. A.,, J. Pardo,, Y. C. Chang,, K. A. Zarember,, G. Nar-done,, E. M. Galvez,, A. Mullenbacher,, J. I. Gallin,, M. M. Simon, and, K. J. Kwon-Chung. 2007b. Gliotoxin is a virulence factor of Aspergillus fumigatus: gliP deletion attenuates virulence in mice immunosuppressed with hydrocortisone. Eukaryot. Cell 6:15621569.
184. Svoboda, P. 2007. Off-targeting and other non-specific effects of RNAi experiments in mammalian cells. Curr. Opin. Mol. Ther. 9:248257.
185. Talbot, G. H.,, J. Bradley,, J. E. Edwards, Jr.,, D. Gilbert,, M. Scheld, and, J. G. Bartlett. 2006. Bad bugs need drugs: an update on the development pipeline from the Antimicrobial Availability Task Force of the Infectious Diseases Society of America. Clin. Infect. Dis. 42:657668.
186. Tang, C. M.,, J. Cohen,, T. Krausz,, S. VanNoorden, and, D. W. Holden. 1993. The alkaline protease of Aspergillus fumigatus is not a virulence factor in two murine models of invasive pulmonary aspergillosis. Infect. Immun. 61:16501656.
187. Tekaia, F., and, J. P. Latge. 2005. Aspergillus fumigatus: saprophyte or pathogen? Curr. Opin. Microbiol. 8:385392.
188. Thau, N.,, M. Monod,, B. Crestani,, C. Rolland,, G. Tronchin,, J. P. Latge, and, S. Paris. 1994. rodletless mutants of Aspergillus fumigatus. Infect. Immun. 62:43804388.
189. Tillie-Leblond, I., and, A.-B. Tonnel. 2005. Allergic bronchopulmonary aspergillosis. Allergy 60:10041013.
190. Tsai, H. F.,, Y. C. Chang,, R. G. Washburn,, M. H. Wheeler, and, K. J. Kwon-Chung. 1998. The developmentally regulated alb1 gene of Aspergillus fumigatus: its role in modulation of conidial morphology and virulence. J. Bacteriol. 180:30313038.
191. Tsai, H. F.,, M. H. Wheeler,, Y. C. Chang, and, K. J. Kwon-Chung. 1999. A developmentally regulated gene cluster Involved in conidial pigment biosynthesis in Aspergillus fumigatus. J. Bacteriol. 181:64696477.
192. Tsitsigiannis, D. I.,, J. W. Bok,, D. Andes,, K. F. Nielsen,, J. C. Frisvad, and, N. P. Keller. 2005. Aspergillus cyclooxygenase-like enzymes are associated with prostaglandin production and virulence. Infect. Immun. 73:45484559.
193. Upton, A.,, K. A. Kirby,, P. Carpenter,, M. Boeckh, and, K. A. Marr. 2007. Invasive aspergillosis following hematopoietic cell transplantation: outcomes and prognostic factors associated with mortality. Clin. Infect. Dis. 44:531540.
194. Valiante, V.,, T. Heinekamp,, R. Jain,, A. Hartl, and, A. A. Brakhage. 2008. The mitogen-activated protein kinase MpkA of Aspergillus fumigatus regulates cell wall signaling and oxidative stress response. Fungal Genet. Biol. 45:618627.
195. Vallor, A. C.,, W. R. Kirkpatrick,, L. K. Najvar,, R. Bocanegra,, M. C. Kinney,, A. W. Fothergill,, M. L. Herrera,, B. L. Wickes,, J. R. Graybill, and, T. F. Patterson. 2008. Assessment of Aspergillus fumigatus burden in pulmonary tissue of guinea pigs by quantitative PCR, galactomannan enzyme immunoassay, and quantitative culture. Antimicrob. Agents Chemother. 52:25932598.
196. van den Brink, J. M.,, P. J. Punt,, R. F. van Gorcom, and, C. A. van den Hondel. 2000. Regulation of expression of the Aspergillus niger benzoate para-hydroxylase cytochrome P450 system. Mol. Gen. Genet. 263:601609.
197. Varga, J. 2003. Mating type gene homologues in Aspergillus fumigatus. Microbiology 149:816819.
198. Vicentefranqueira, R.,, M. A. Moreno,, F. Leal, and, J. A. Calera. 2005. The zrfA and zrfB genes of Aspergillus fumigatus encode the zinc transporter proteins of a zinc uptake system induced in an acid, zinc-depleted environment. Eukaryot. Cell 4:837848.
199. Vilela, C.,, B. Linz,, C. Rodrigues-Pousada, and, J. E. McCarthy. 1998. The yeast transcription factor genes YAP1 and YAP2 are subject to differential control at the levels of both translation and mRNA stability. Nucleic Acids Res. 26:11501159.
200. Vilela, C., and, J. E. McCarthy. 2003. Regulation of fungal gene expression via short open reading frames in the mRNA 5´untranslated region. Mol. Microbiol. 49:859867.
201. Vogt, K.,, R. Bhabhra,, J. C. Rhodes, and, D. S. Askew. 2005. Doxycycline-regulated gene expression in the opportunistic fungal pathogen Aspergillus fumigatus. BMC Microbiol. 5:1.
202. Walsh, T. J.,, E. J. Anaissie,, D. W. Denning,, R. Herbrecht,, D. P. Kontoyiannis,, K. A. Marr,, V. A. Morrison,,