Chapter 44 : : Budding Yeast and Dimorphic Filamentous Fungus

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and are closely related basidiomycetous fungi that commonly infect humans to predominantly cause meningoencephalitis. Both grow as budding yeasts in the environment and in the infected host yet undergo a dimorphic transition to a filamentous monokaryon or dikaryon during sexual reproduction. This chapter covers recent exciting advances in the field with special consideration of features of the virulence and life cycle relevant to studies of filamentous fungi and the emergence of microbial pathogens successfully infecting animals. The iron regulatory network and iron acquisition functions have been examined in some detail for . Initially, the response of the fungus to iron deprivation was examined by transcriptional profiling, and this study identified general patterns of gene expression as well as specific iron-responsive functions. The latter genes encoded iron acquisition functions and a predicted mannoprotein described as a cytokine-inducing glycoprotein (Cig1). The availability of the genome sequences and molecular techniques for strains provides an opportunity to define the transcriptome of the pathogen under a variety of growth conditions. An interesting recent study on extracellular proteome targeted proteins associated with extracellular vesicles. The contribution of the α allele to pathogenicity is background dependent, and virulence is a quantitative trait, in which mating-type locus () interacts with other unlinked genes to contribute to virulence.

Citation: Kronstad J, Lodge J, Heitman J. 2010. : Budding Yeast and Dimorphic Filamentous Fungus, p 717-735. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch44

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Image of FIGURE 1

The morphology of sexual reproduction in . A diagram representing the stages in sexual reproduction for (center) is surrounded by images showing representative stages in the process. Two haploid cells of opposite mating types (α and ) respond to a panoply of appropriate environmental cues (including nutrient limitation, the presence of inositol and copper ions, desiccation, darkness, low temperature, low levels of carbon dioxide, surface growth, the presence of plants, or growth on pigeon guano) that stimulate pheromone production and early morphological changes including conjugation tube formation that lead to cell-cell fusion. Following cell-cell fusion, the nuclei congress, but nuclear fusion is delayed, and the resulting dikaryon switches from growth as a budding yeast to growth as a dikaryotic hyphae. Unknown signals trigger the production of terminal fruiting structures, the basidia, wherein karyogamy and meiosis occur, and long chains of infectious basidiospores are then produced by basipetal budding from the basidium. Germination of spores produces haploid meiotic products that return to the budding yeast growth mode. Images show (counterclockwise from the lower right hand corner) (i) microcolonies producing conjugation tubes oriented towards dikaryotic hyphae from a mating mixture as a source of pheromones (left panel) and microcolonies on the surface of V8 mating medium linked by conjugation tubes; larger dikaryotic filaments are also seen emanating from the central microcolony of cells as a result of cell-cell fusion mediated by conjugation tubes; (ii) confrontation assay on filament agar medium showing the formation of conjugation tubes and monokaryotic fruiting by the α partner and the production of enlarged cells by the partner; (iii) fusion assay, in which the production of prototrophic dikaryons or diploids following cell-cell fusion is monitored by growth on minimal medium lacking lysine and adenine; (iv) dikaryotic filament with a terminal basidium decorated with four long intertwined spore chains; (v) edge of a mating mixture on V8 mating medium showing profuse dikaryotic hyphae with terminal basidia and spore chains (not resolved at this magnification).

Citation: Kronstad J, Lodge J, Heitman J. 2010. : Budding Yeast and Dimorphic Filamentous Fungus, p 717-735. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch44
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Image of FIGURE 2

mating partners respond to mating pheromones during confrontational growth. The diagram depicts morphological events transpiring during the early events in sexual reproduction that can be detected using a confrontation assay in which mating partners are grown in close proximity but not touching on V8, SLAD (super low ammonium dextrose), or filamentation agar. With serotype D strains, α cells respond to pheromone to form conjugation tubes and then undergo monokaryotic fruiting, which may serve as a response to locate more-distant mating partners. By contrast, cells often undergo an isotropic expansion in response to α pheromone to form enlarged cells, possibly to serve as targets for fusion by conjugation tubes or hyphae produced by the α mating partner. Under other conditions, or with other isolates, cells can also be observed producing conjugation tubes in response to α pheromone.

Citation: Kronstad J, Lodge J, Heitman J. 2010. : Budding Yeast and Dimorphic Filamentous Fungus, p 717-735. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch44
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Image of FIGURE 3

Clamp cells ensure faithful nuclear segregation during dikaryotic hyphal growth. During hyphal growth of the dikaryon, a specialized cell known as a clamp cell plays a central role in ensuring that each hyphal cell receives one copy of each nucleus. Stages in clamp cell formation, nuclear migration and division, and clamp cell fusion are depicted. Based on studies in other basidiomycetous fungi, it is hypothesized that pheromone production and sensing are involved in the clamp cell-hyphal cell fusion events. Nuclear fusion (karyogamy) is depicted occurring in the terminal basidium, followed by meiosis and mitotic production of long chains of basidiospores. The two opposite-mating-type nuclei are depicted with filled and solid circles.

Citation: Kronstad J, Lodge J, Heitman J. 2010. : Budding Yeast and Dimorphic Filamentous Fungus, p 717-735. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch44
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Image of FIGURE 4

Mitochondria are inherited in a uniparental fashion during sexual reproduction. Directed growth of the conjugation tube from an α mating partner towards the recipient mating partner is depicted. Mitochondria are depicted with oval symbols; shaded symbols indicate those from the parent, and open symbols depict those from the α parent. Analysis of mitochondrial genotypes from meiotic progeny produced by sexual reproduction reveals uniparental inheritance from the parent. Mitochondria from the α parent either may be left behind if only the nucleus migrates through the conjugation tube or may be actively destroyed, or both. Recombination between mitochondrial genomes has also been observed in some defined genetic crosses.

Citation: Kronstad J, Lodge J, Heitman J. 2010. : Budding Yeast and Dimorphic Filamentous Fungus, p 717-735. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch44
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Image of FIGURE 5

Sexual reproduction of involves both -α opposite-sex mating and α-α unisexual mating. The lower panel depicts the well-established heterothallic sexual cycle involving and α mating partners, which fuse to produce a filamentous dikaryon that forms terminal basidia and undergoes meiosis to produce a 1:1 mixture of basidiospores of and α mating types. However, a central conundrum in the field has been the vast disparity in the distribution and prevalence of the two mating types, with α being significantly more common globally. The upper panel depicts a newly discovered sexual cycle involving only α cells, known as monokaryotic fruiting, same-sex mating, or unisexual reproduction. Similar environmental conditions stimulate opposite-sex and same-sex mating. During same-sex mating, α cells can fuse with other α cells or possibly also undergo other forms of diploidization such as endoreplication. Hyphal growth of the resulting diploid isolates is often enhanced and leads to the formation of monokaryotic hyphae with unfused clamp connections, terminal basidia, meiosis, and chains of only α basidiospores. Diploidization can occur early in the differentiation pathway or in some isolates may occur late, possibly only in the basidium, similar to the heterothallic sexual cycle. This unusual homothallic unisexual cycle may have arisen as a consequence of the largely unisexual population or may have driven the success of the α mating type. Some isolates of mating type have been found to undergo same-sex mating. Recent studies reveal that same-sex mating is a quantitative trait controlled by many segregating polymorphic genetic loci. In particular, the locus is one of the most significant quantitative trait loci influencing unisexual mating, and the α allele promotes fruiting to a greater extent than the allele, again providing insight into why the α allele may be the predominant form found in nature.

Citation: Kronstad J, Lodge J, Heitman J. 2010. : Budding Yeast and Dimorphic Filamentous Fungus, p 717-735. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch44
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Properties of the three varieties of

Citation: Kronstad J, Lodge J, Heitman J. 2010. : Budding Yeast and Dimorphic Filamentous Fungus, p 717-735. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch44
Generic image for table

Genome sequencing projects for and

Citation: Kronstad J, Lodge J, Heitman J. 2010. : Budding Yeast and Dimorphic Filamentous Fungus, p 717-735. In Borkovich K, Ebbole D (ed), Cellular and Molecular Biology of Filamentous Fungi. ASM Press, Washington, DC. doi: 10.1128/9781555816636.ch44

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