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Color Plates

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Figures

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COLOR PLATE 1

PTX3 is stored in neutrophil-specific granules. Human PMNs from peripheral blood were stained for PTX3 (green), lactoferrin (red), and DNA (Hoechst 33258) and analyzed by confocal microscopy. (Inset) Enlargement of the indicated area with colocalization of PTX3 with lactoferrin.

Citation: Russell D, Gordon S. 2009. Color Plates, In Phagocyte-Pathogen Interactions. ASM Press, Washington, DC.
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COLOR PLATE 2

PTX3 is localized in NETs. Neutrophils were exposed to 100 ng/ml LPS for 40 min. PTX3 (green) and DNA (red) staining was done on nonpermeabilized neutrophils. A differential interference contrast (Nomarski technique) is shown in the right panels.

Citation: Russell D, Gordon S. 2009. Color Plates, In Phagocyte-Pathogen Interactions. ASM Press, Washington, DC.
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COLOR PLATE 3

The movements of phagocytosis. The diagram shows a time series of the essential cellular movements during phagocytosis, viewed as a sagittal section. Binding of an opsonized particle (solid gray oval) to the phagocyte stimulates polymerization of actin (gray lines) and the extension of plasma membrane (black lines) as a cup-shaped phagocytic pseudopod. As the phagocytic cup extends outward around the particle, intracellular vesicles fuse with plasma membrane, and actin is cleared at the base of the cup. The phagosome closes at its distal margin into a discrete intracellular vacuole. Enlargements show the dynamics of essential components during pseudopod extension. As the pseudopod extends over the particle, receptors (red) diffusing in the plasma membrane engage cognate ligands (black) on the particle surface. Pseudopod extension is mediated in part by actin (green) polymerization at the distal margin of the phagocytic cup. Older actin filaments toward the base of the cup depolymerize. Several classes of myosin (blue) generate inward, circumferential contractile forces that constrict the phagosome and possibly additional contractile forces that pull the actin meshwork toward the distal margin. Phagosomal membranes derive from plasma membrane and intracellular vesicles that fuse in or near the phagocytic cup.

Citation: Russell D, Gordon S. 2009. Color Plates, In Phagocyte-Pathogen Interactions. ASM Press, Washington, DC.
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COLOR PLATE 4

Short- and medium-range signaling in FcR-mediated phagocytosis. An enlarged region of the inner membrane of a phagocytic cup contains ligated and nonligated FcRs. Short-range signals that follow FcR binding to IgG include chemical modifications of the receptors, as well as the enzymes (blue circles) and lipids (blue lines) directly associated with the receptors. Lipids of the inner leaflet of the membrane are modified by receptor-associated enzymes. Diffusion of these lipids away from the receptors (red lines) creates a local area of modified membrane that can recruit or activate PH domain-containing proteins that recognize those modified lipids. Such medium-range signaling can report the collective activities of many receptors, thereby coordinating or integrating the signals from multiple receptors.

Citation: Russell D, Gordon S. 2009. Color Plates, In Phagocyte-Pathogen Interactions. ASM Press, Washington, DC.
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COLOR PLATE 5

Phosphatidylserine (PS) and calreticulin are exposed together in patches on apoptotic cells. An apoptotic neutrophil is depicted with surface PS (green) detected with fluorescent factor Va and calreticulin (red) with anticalreticulin antibody. (From Gardai et al., 2005.)

Citation: Russell D, Gordon S. 2009. Color Plates, In Phagocyte-Pathogen Interactions. ASM Press, Washington, DC.
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COLOR PLATE 6

Phagocytosis of apoptotic cells; efferocytosis. Mouse resident peritoneal macrophage phagocytosing apoptotic (dexamethasone-treated) thymocytes. Blue = nuclei (Hoechst), green = Cell Tracker Green (macrophage cytosol only), red = anti-12/15-lipoxygenase, which in this micrograph is seen in the extended membrane ruffle. (Yury Miller, University of California, San Diego.)

Citation: Russell D, Gordon S. 2009. Color Plates, In Phagocyte-Pathogen Interactions. ASM Press, Washington, DC.
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COLOR PLATE 7

The SPI-2 T3SS enables replication in macrophages. Mouse macrophage-like RAW cells were infected with green fluorescent protein-expressing wild-type or SPI-2 T3SS null () mutant for 8 h. Fluorescence and differential interference contrast images of the same cells are superimposed. Scale bar, 5 μm.

Citation: Russell D, Gordon S. 2009. Color Plates, In Phagocyte-Pathogen Interactions. ASM Press, Washington, DC.
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COLOR PLATE 8

Structure of the cell wall of . On the left is a transmission electron microscopy image clearly showing the fibrillar mannoprotein outer layer (scale bar is 100 μm); on the right is a cartoon representation of the major cell wall components. Image courtesy of Neil Gow (University of Aberdeen).

Citation: Russell D, Gordon S. 2009. Color Plates, In Phagocyte-Pathogen Interactions. ASM Press, Washington, DC.
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COLOR PLATE 9

Recruitment of an ER-associated marker with the replication vacuole. Fluorescent micrographs of challenged with either (A) or (B) an mutant having a nonfunctional Dot/Icm apparatus. Amoebae were challenged for 6 h with fixed and stained with anti- (red). The amoebae express a GFP-HDEL fusion protein (green stain) that has a 4-amino-acid, carboxyl-terminal ER-retention signal that allows labeling of the ER and intermediate compartments in the host cell. Note the large ring of GDP-HDEL about wild-type bacteria missing in the micrograph of the mutant. Micrograph is from Li et al. (2005).

Citation: Russell D, Gordon S. 2009. Color Plates, In Phagocyte-Pathogen Interactions. ASM Press, Washington, DC.
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COLOR PLATE 10

Larval zebrafish macrophages infected with in vivo. (A) Single macrophage infected with green fluorescent , surrounded by caudal hematopoietic tissue (dorsal is up). Immediately below is the caudal vein, where nucleated erythrocytes are flowing to the left (anterior). ~24 hpi. (B) Granuloma in the brain of a 5-day embryo. Green fluorescent seen inside both living and dead (nonmotile) macrophages. Neuronal tissue is visible to the lower right. (Scale bar, 20 μm; both panels, same scale.)

Citation: Russell D, Gordon S. 2009. Color Plates, In Phagocyte-Pathogen Interactions. ASM Press, Washington, DC.
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