Chapter 105 : Poxviruses

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This chapter deals with poxviruses belonging to the family and subfamily . The G+C contents of orthopoxviruses, yatapoxviruses, (MCV), and parapoxviruses are ~33, ~32, ~60, and ~63%, respectively. The zoonotic poxviruses include members of the genera (monkeypox virus, cowpox virus, and the vaccinia virus subspecies, including buffalopox virus), (orf, pseudocowpox, sealpox, and papulosa stomatitis viruses), and (tanapox virus [TPV], Yaba monkey tumor virus [YMTV], and Yaba-like disease virus [YLDV]). Poxviruses produce inclusions that have characteristic appearances when stained with May-Grunwald Giemsa and hematoxylin-eosin stains. Perinuclear basophilic or B-type cytoplasmic inclusions (virus factories or viroplasm) are observed with cells infected with any of the poxviruses and represent sites of virus replication. Polymerase chain reaction (PCR) analysis is used by the WHO collaborating center (WHOCC) at the center for disease control (CDC) to detect poxvirus DNA in samples. Orthopoxviruses are the only human poxviruses that produce pocks on the chorioallantoic membranes (CAMs) of fertile chicken eggs; pock morphology is useful for biologic species and variant differentiation. Parapoxviruses, yatapoxviruses, and MCV do not form pocks on the CAM, although avipoxviruses, leporipoxviruses, and capripoxviruses do so. Serologic methods currently used to detect antibodies against human orthopoxviruses include enzyme-linked immunosorbent assays (ELISAs), the virus neutralization test (NT), Western blotting, and hemagglutination inhibition. In the current state of bioterrorism response awareness, tests to evaluate residual protection from previous vaccination are being requested.

Citation: Damon I. 2011. Poxviruses, p 1647-1658. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch105

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Yaba monkey tumor virus
Restriction Fragment Length Polymorphism
Random Amplified Polymorphic DNA
Molluscum contagiosum virus
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Image of FIGURE 1

A negative-stain electron micrograph demonstrates the two forms of the brick-shaped monkeypox virus from a cell culture. The surfaces of M (mulberry) virions are covered with short, whorled filaments, while C (capsular)-form virions penetrated by stain present as a sharply defined, dense core surrounded by several laminated zones of differing densities. (Image 3945 from the CDC Public Health Image Library; courtesy of C. Goldsmith, I. Damon, and S. Zaki.)

Citation: Damon I. 2011. Poxviruses, p 1647-1658. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch105
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Image of FIGURE 2

Vaccinia virus (“Dryvax”) CPE in FHRK-4 cells. (A) Early CPE; (B) mature CPE. (Courtesy of V. Olson.)

Citation: Damon I. 2011. Poxviruses, p 1647-1658. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch105
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Image of FIGURE 3

Monkeypox virus (Congo Basin clade, v79-I-005) CPE in FRHK-4 cells. (A) Early CPE; (B) mature CPE with syncytia. (Courtesy of V. Olson.)

Citation: Damon I. 2011. Poxviruses, p 1647-1658. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch105
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1. Aitichou, M.,, S. Javorschi,, and M. S. Ibrahim. 2005. Twocolor multiplex assay for the identification of orthopox viruses with real-time LUX-PCR. Mol. Cell. Probes 19:323328.
2. Baxby, D.,, M. Bennett,, and B. Getty. 1994. Human cowpox 1969-93: a review based on 54 cases. Br. J. Dermatol. 131:598607.
3. Bennett, M.,, A. J. Crouch,, M. Begon,, B. Duffy,, S. Feore,, R. M. Gaskell,, D. F. Kelly,, C. M. McCracken,, L. Vicary,, and D. Baxby. 1997. Cowpox in British voles and mice. J. Comp. Pathol. 116:3544.
4. Bennett, M.,, C. J. Gaskill,, D. Baxby,, R. M. Gaskill,, D. F. Kelly,, and J. Naidoo. 1990. Feline cowpox virus infection. A review. J. Small Anim. Pract. 14:167173.
5. Breman, J. G., 2000. Monkeypox: an emerging infection for humans?, p. 4567. In W. M. Scheld,, W. A. Craig,, and J. M. Hughes (ed.), Emerging Infections 4. ASM Press, Washington, DC.
6. Carletti F.,, A. Di Caro,, S. Calcaterra,, A. Grolla,, M. Czub,, G. Ippolito,, M. R. Capobianchi,, and D. Horejsh. 2005. Rapid, differential diagnosis of orthopox- and herpesviruses based upon real-time PCR product melting temperature and restriction enzyme analysis of amplicons. J. Virol. Methods 129:97100.
7.Centers for Disease Control and Prevention. 1997. Human monkeypox—Kasai Oriental, Democratic Republic of Congo, February 1996-October 1997. MMWR Morb. Mortal. Wkly. Rep. 46:11681171.
8.Centers for Disease Control and Prevention. 1997. Human monkeypox—Kasai Oriental, Zaire, 1996-1997. MMWR Morb. Mortal. Wkly. Rep. 46:304307.
9.Centers for Disease Control and Prevention. 2003. Update: multistate outbreak of monkeypox—Illinois, Indiana, Kansas, Missouri, Ohio, and Wisconsin, 2003. MMWR Morb. Mortal. Wkly. Rep. 52:642646.
10.Centers for Disease Control and Prevention. 2001. Vaccinia (smallpox) vaccination: recommendations of the Advisory Committee on Immunization Practices (ACIP). MMWR Morb. Mortal. Wkly. Rep. 50:122.
11.Centers for Disease Control and Prevention. 2009. Progressive vaccinia in a military smallpox vaccinee—United States, 2009. MMWR Morb. Mortal. Wkly. Rep. 58:532536.
12.Centers for Disease Control and Prevention. 2009. Human vaccinia infection after contact with a raccoon rabies vaccine bait—Pennsylvania, 2009. MMWR Morb. Mortal. Wkly. Rep. 58:12041207.
13. Chen, N.,, G. Li,, M. K. Liszewski,, J. P. Atkinson,, P. B. Jahrling,, Z. Feng,, J. Schriewer,, C. Buck,, C. Wang,, E. J. Lefkowitz,, J. J. Esposito,, T. Harms,, I. K. Damon,, R. L. Roper,, C. Upton,, and R. M. Buller. 2005. Virulence differences between monkeypox virus isolates from West Africa and the Congo basin. Virology 340:4663.
14. Clark, C.,, P. G. McIntyre,, A. Evans,, C. J. McInnes,, and S. Lewis-Jones. 2005. Human sealpox resulting from a seal bite: confirmation that sealpox virus is zoonotic. Br. J. Dermatol. 152:791793.
15. Cole, G. A.,, and R. V. Blanden,. 1982. Immunology of poxviruses, p. 119. In A. J. Nahmias, and R. J. O’Reilly (ed.), Comprehensive Immunology, vol. 9. Plenum Press, New York, NY.
16. Conroy, J. M.,, R. W. Stevens,, and K. E. Hechemy,. 1991. Enzyme immunoassays, p. 8792. In A. Balows,, W. J. Hausler,, K. L. Herrmann,, H. D. Isenberg,, and H. J. Shadomy (ed.), Manual of Clinical Microbiology, 5th ed. American Society for Microbiology, Washington, DC.
17. da Fonseca, F. G.,, G. S. Trindade,, R. L. Silva,, C. A. Bonjardim,, P. C. Ferreira,, and E. G. Kroon. 2002. Characterization of a vaccinia-like virus isolated in a Brazilian forest. J. Gen. Virol. 83:223228.
18. Damaso, C. R.,, J. J. Esposito,, R. C. Condit,, and N. Moussatche. 2000. An emergent poxvirus from humans and cattle in Rio de Janeiro State: Cantagalo virus may derive from Brazilian smallpox vaccine. Virology 277:439449.
19. Damon, I., 2007. Poxviruses, p. 29472977. In D. M. Knipe,, P. M. Howley, et al. (ed.), Fields Virology, 5th ed. Lippincott Williams and Wilkins, New York, NY.
20. Dhar, A. D.,, A. E. Werchniak,, Y. Li,, J. B. Brennick,, C. S. Goldsmith,, R. Kline,, I. Damon,, and S. N. Klaus. 2004. Tanapox infection in a college student. N. Engl. J. Med. 350:361366.
21. Dumbell, K. R.,, and F. Huq. 1986. The virology of variola minor. Correlation of laboratory tests with the geographic distribution and human virulence of variola isolates. Am. J. Epidemiol. 123:403415.
22. Dumbell, K. R.,, and M. Richardson. 1993. Virological investigations of specimens from buffaloes affected by buffalopox in Maharashtra State, India between 1985 and 1987. Arch. Virol. 128:257267.
23. Edghill-Smith, Y.,, H. Golding,, J. Manischewitz,, L. R. King,, D. Scott,, M. Bray,, A. Nalca,, J. W. Hooper,, C. A. Whitehouse,, J. E. Schmitz,, K. A. Reimann,, and G. Franchini. 2005. Smallpox vaccine-induced antibodies are necessary and sufficient for protection against monkeypox virus. Nat. Med. 11:740747.
24. Espy, M. J.,, F. R. Cockerill III,, R. F. Meyer,, M. D. Bowen,, G. A. Poland,, T. L. Hadfield,, and T. F. Smith. 2002. Detection of smallpox virus DNA by LightCycler PCR. J. Clin. Microbiol. 40:19851988.
25. Fedele, C. G.,, A. Negredo,, F. Molero,, M. P. Sánchez-Seco,, and A. Tenorio. 2006. Use of internally controlled realtime genome amplification for detection of variola virus and other orthopoxviruses infecting humans. J. Clin. Microbiol. 44:44644470.
26. Fedorko, D. P.,, J. C. Preuss,, G. A. Fahle,, L. Li,, S. H. Fischer,, P. Hohman,, and J. I. Cohen. 2005. Comparison of methods for detection of vaccinia virus in patient specimens. J. Clin. Microbiol. 43:46024606.
27. Fenner, F.,, D. A. Henderson,, I. Arita,, Z. Jezek,, and I. Ladnyi. 1988. Smallpox and Its Eradication. World Health Organization, Geneva, Switzerland.
28. Fenner, F.,, and J. H. Nakano,. 1988. Poxviridae: the poxviruses, p. 177207. In E. H. Lennette,, P. Halonen,, and F. A. Murphy (ed.), Laboratory Diagnosis of Infectious Diseases. vol. 2. Viral, Rickettsial, and Chlamydial Diseases. Springer-Verlag, New York, NY.
29. Fenner, F.,, R. Wittek,, and K. R. Dumbell. 1989. The Orthopoxviruses. Academic Press, Inc., New York, NY.
30. Gallina, L.,, F. Dal Pozzo,, C. J. Mc Innes,, G. Cardeti,, A. Guercio,, M. Battilani,, S. Ciulli,, and A. Scagliarini. 2006. Orf virus detection by real time PCR. J. Virol. Methods 134:140145.
31. Gelderblom, H. R.,, and P. R. Hazelton. 2000. Specimen collection for electron microscopy. Emerg. Infect. Dis. 6:433434.
32. Hammarlund, E.,, M. W. Lewis,, S. V. Carter,, I. Amanna,, S. G. Hansen,, L. I. Strelow,, S. W. Wong,, P. Yoshihara,, J. M. Hanifin,, and M. K. Slifka. 2005. Multiple diagnostic techniques identify previously vaccinated individuals with protective immunity against monkeypox. Nat. Med. 11:105111.
33. Huggins, J.,, A. Goff,, L. Hensley,, E. Mucker,, J. Shamblin,, C. Wlazlowski,, W. Johnson,, J. Chapman,, T. Larsen,, N. Twenhafel,, K. Karem,, I. K. Damon,, C. M. Byrd,, T. C. Bolken,, R. Jordan,, and D. Hruby. 2009. Nonhuman primates are protected from smallpox virus or monkeypox virus challenges by the antiviral drug ST-246. Antimicrob. Agents Chemother. 53:26202625.
34. Huhn, G. D.,, A. M. Bauer,, K. Yorita,, M. B. Graham,, J. Sejvar,, A. Likos,, I. K. Damon,, M. G. Reynolds,, and M. J. Kuehnert. 2005. Clinical characteristics of human monkeypox, and risk factors for severe disease. Clin. Infect. Dis. 41:17421751.
35. Hutin, Y. J.,, R. J. Williams,, P. Malfait,, R. Pebody,, V. N. Loparev,, S. L. Ropp,, M. Rodriguez,, J. C. Knight,, F. K. Tshioko,, A. S. Khan,, M. V. Szczeniowski,, and J. J. Esposito. 2001. Outbreak of human monkeypox, Democratic Republic of Congo, 1996 to 1997. Emerg. Infect. Dis. 7:434438.
36. Ibrahim, M. S.,, D. A. Kulesh,, S. S. Saleh,, I. K. Damon,, J. J. Esposito,, A. L. Schmaljohn,, and P. B. Jahrling. 2003. Realtime PCR assay to detect smallpox virus. J. Clin. Microbiol. 41:38353839.
37. Jezek, Z.,, I. Arita,, M. Szczeniowski,, K. M. Paluku,, K. Ruti,, and J. H. Nakano. 1985. Human tanapox in Zaire: clinical and epidemiological observations on cases confirmed by laboratory studies. Bull. W. H. O. 63:10271035.
38. Jezek, Z.,, and F. Fenner. 1988. Human monkeypox. Monogr. Virol. 17:1140.
39. Karem, K. L.,, M. Reynolds,, Z. Braden,, G. Lou,, N. Bernard,, J. Patton,, and I. K. Damon. 2005. Characterization of acutephase humoral immunity to monkeypox: use of immunoglobulin M enzyme-linked immunosorbent assay for detection of monkeypox infection during the 2003 North American outbreak. Clin. Diagn. Lab. Immunol. 12:867872.
40. Kelly, C. D.,, C. Egan,, S. W. Davis,, W. A. Samsonoff,, K. A. Musser,, P. Drabkin,, J. R. Miller,, J. Taylor,, and N. M. Cirino. 2004. Laboratory confirmation of generalized vaccinia following smallpox vaccination. J. Clin. Microbiol. 42:13731375.
41. Knight, J. C.,, F. J. Novembre,, D. R. Brown,, C. S. Goldsmith,, and J. J. Esposito. 1989. Studies on tanapox virus. Virology 172:116124.
42. Kulesh, D. A.,, R. O. Baker,, B. M. Loveless,, D. Norwood,, S. H. Zwiers,, E. Mucker,, C. Hartmann,, R. Herrera,, D. Miller,, D. Christensen,, L. P. Wasieloski, Jr.,, J. Huggins, and P. B. Jahrling. 2004. Smallpox and pan-orthopox virus detection by real-time 39-minor groove binder TaqMan assays on the Roche LightCycler and the Cepheid smart Cycler platforms. J. Clin. Microbiol. 42:601609.
43. Lapa, S.,, M. Mikheev,, S. Shchelkunov,, V. Mikhailovich,, A. Sobolev,, V. Blinov,, I. Babkin,, A. Guskov,, E. Sokunova,, A. Zasedatelev,, L. Sandakhchiev,, and A. Mirzabekov. 2002. Species-level identification of orthopoxviruses with an oligonucleotide microchip. J. Clin. Microbiol. 40:753757.
44. Learned, L. A.,, M. G. Reynolds,, D. W. Wassa,, Y. Li,, V. A. Olson,, K. Karem,, L. L. Stempora,, Z. H. Braden,, R. Kline,, A. Likos,, F. Libama,, H. Moudzeo,, J. D. Bolanda,, P. Tarangonia,, P. Boumandoki,, P. Formenty,, J. M. Harvey,, and I. K. Damon. 2005. Extended interhuman transmission of monkeypox in a hospital community in the Republic of the Congo, 2003. Am. J. Trop. Med. Hyg. 73:428434.
45. Lewis, F. M. T.,, E. Chernak,, E. Goldmann,, Y. Li,, K. Karem,, I. K. Damon,, R. Henkel,, E. C. Newbern,, P. Ross,, and C. C. Johnson. 2006. Ocular vaccinia infection in laboratory worker, Philadelphia, 2004. Emerg. Infect. Dis. 12:134137.
46. Li, Y.,, V. A. Olson,, T. Laue,, M. T. Laker,, and I. K. Damon. 2006. Detection of monkeypoxvirus with real time PCR assays. J. Clin. Virol. 36:194203.
47. Li, Y.,, H. Meyer,, H. Zhao,, and I. K. Damon. 2010. GC content-based universal PCR assays for poxvirus detection. J. Clin. Microbiol. 48:268276.
48. Likos, A. M.,, S. A. Sammons,, V. A. Olson,, A. M. Frace,, Y. Li,, M. Olsen-Rasmussen,, W. Davidson,, R. Galloway,, M. L. Khristova,, M. G. Reynolds,, H. Zhao,, D.S. Carroll,, A. Curns,, P. Formenty,, J. J. Esposito,, R. L. Regnery,, and I. K. Damon. 2005. A tale of two clades: monkeypox viruses. J. Gen. Virol. 86:26612672.
49. Long, G. W.,, J. Nobel, Jr.,, F. A. Murphy,, K. L. Herrmann, and B. Lourie. 1970. Experience with electron microscopy in the differential diagnosis of smallpox. Appl. Microbiol. 20: 497504.
50. Loparev, V. N.,, R. F. Massung,, J. J. Esposito,, and H. Meyer. 2001. Detection and differentiation of Old World orthopoxviruses: restriction fragment length polymorphism of the crmB gene region. J. Clin. Microbiol. 39:94100.
51. Marques, J. T.,, G. D. Trindade,, F. G. Da Fonseca,, J. R. Dos Santos,, C. A. Bonjardim,, P. C. Ferreira,, and E. G. Kroon. 2001. Characterization of ATI, TK and IFN-alpha/betaR genes in the genome of the BeAn 58058 virus, a naturally attenuated wild orthopoxvirus. Virus Genes 23:291301.
52. Mathew, T. 1987. Advances in Medical and Veterinary Virology, Immunology and Epidemiology: Cultivation and Immunological Studies on Pox Group of Viruses with Special Reference to Buffalo Pox Virus. Thajema Publishers, New Delhi, India.
53. Meyer, H.,, M. Perrichot,, M. Stemmler,, P. Emmerich,, H. Schmitz,, F. Varaine,, R. Shungu,, F. Tshioko,, and P. Formenty. 2002. Outbreaks of disease suspected of being due to human monkeypox virus infection in the Democratic Republic of Congo in 2001. J. Clin. Microbiol. 40:29192921.
54. Meyer, H.,, S. L. Ropp,, and J. J. Esposito. 1997. Gene for Atype inclusion body protein is useful for a polymerase chain reaction assay to differentiate orthopoxviruses. J. Virol. Methods 64:217221.
55. Meyer, H.,, S. L. Ropp,, and J. J. Esposito,. 1998. Poxviruses, p. 199211. In A. Warnes, and J. Stephenson (ed.), Methods in Molecular Medicine: Diagnostic Virology Protocols. Humana Press, Totowa, NJ.
56. Moriello, K. A.,, and J. Cooley. 2001. Difficult dermatologic diagnosis. Contagious viral pustular dermatitis (orf), goatpox, dermatophilosis, dermatophytosis, bacterial pyoderma, and mange. J. Am. Vet. Med. Assoc. 218:1920.
57. Moss, B., 2007. Poxviridae and their replication, p. 29052947. In D. M. Knipe,, P. M. Howley, et al. (ed.), Fields Virology, 5th ed. Lippincott Williams & Wilkins, New York, NY.
58. Moss, B. 2006. Poxvirus entry and membrane fusion. Virology 344:4854.
59. Nagasse-Sugahara, T. K.,, J. J. Kisielius,, M. Ueda-Ito,, S. P. Curti,, C. A. Figueiredo,, A. S. Cruz,, M. M. Silva,, C. H. Ramos,, M. C. Silva,, T. Sakurai,, and L. F. Salles-Gomes. 2004. Human vaccinia-like virus outbreaks in Sao Paulo and Goias States, Brazil: virus detection, isolation and identification. Rev. Inst. Med. Trop. Sao Paulo 46:315322.
60. Nakano, J. H., 1978. Comparative diagnosis of poxvirus diseases, p. 267339. In E. Kurstak, and C. Kurstak (ed.), Comparative Diagnosis of Viral Diseases, vol. 1. Academic Press, Inc., New York, NY.
61. Nakano, J. H., 1979. Poxviruses, p. 257308. In E. H. Lennette, and N. J. Schmidt (ed.), Diagnostic Procedures for Viral, Rickettsial, and Chlamydial Infections, 5th ed. American Public Health Association, Inc., Washington, DC.
62. Nalca, A.,, A. W. Rimoin,, S. Bavari,, and C. A. Whitehouse. 2005. Reemergence of monkeypox: prevalence, diagnostics, and countermeasures. Clin. Infect. Dis. 41:17651771.
63. Niedrig, M.,, H. Meyer,, M. Panning,, and C. Drosten. 2006. Follow-up on diagnostic proficiency of laboratories equipped to perform orthopoxvirus detection and quantification by PCR: the second international external quality assurance study. J. Clin. Microbiol. 44:12831287.
64. Nitsche, A.,, H. Ellerbrok,, and G. Pauli. 2004. Detection of orthopoxvirus DNA by real-time PCR and identification of variola virus DNA by melting analysis. J. Clin. Microbiol. 42:12071213.
65. Nitsche, A.,, B. Steger,, H. Ellerbrok,, and G. Pauli. 2005. Detection of vaccinia virus DNA on the LightCycler by fluorescence melting curve analysis. J. Virol. Methods 126:187195.
66. Nitsche, A.,, M. Büttner,, S. Wilhelm,, G. Pauli,, and H. Meyer. 2006. Real-time PCR detection of parapoxvirus DNA. Clin. Chem. 52:316319.
67. Nunez, A.,, J. M. Funes,, M. Agromayor,, M. Moratilla,, A. J. Varas,, J. L. Lopez-Estebaranz,, M. Esteban,, and A. Martin-Gallardo. 1996. Detection and typing of molluscum contagiosum virus in skin lesions by using a simple lysis method and polymerase chain reaction. J. Med. Virol. 50:342349.
68. Olson, V. A.,, T. Laue,, M. T. Laker,, I. V. Babkin,, C. Drosten,, S. N. Shchelkunov,, M. Niedrig,, I. K. Damon,, and H. Meyer. 2004. Real-time PCR system for detection of orthopoxviruses and simultaneous identification of smallpox virus. J. Clin. Microbiol. 42:19401946.
69. Panning, M.,, M. Asper,, S. Kramme,, H. Schmitz,, and C. Drosten. 2004. Rapid detection and differentiation of human pathogenic orthopox viruses by a fluorescence resonance energy transfer real-time PCR assay. Clin. Chem. 50:702708.
70. Pepose, J. S.,, and J. J. Esposito,. 1996. Molluscum contagiosum, Orf, and vaccinia virus ocular infections in humans, p. 846856. In J. S. Pepose,, G. N. Holland,, and K. R. Wilhelmus (ed.), Ocular Infections and Immunity. Mosby Year Book, Inc., St. Louis, MO.
71. Porter, C. D.,, N. W. Blake,, J. J. Cream,, and L. C. Archard,. 1992. Molluscum contagiosum virus, p. 233257. In D. Wright, and L. C. Archard (ed.), Molecular and Cellular Biology of Sexually Transmitted Diseases. Chapman and Hall, London, England.
72. Putkuri, N.,, H. Piiparinen,, A. Vaheri,, and O. Vapalahti. 2009. Detection of human orthopoxvirus infections and differentiation of smallpox virus with real time PCR. J. Med. Virol. 81:146152.
73. Reed, K. D.,, J. W. Melski,, M. B. Graham,, R. L. Regnery,, M. J. Sotir,, M. V. Wegner,, J. J. Kazmierczak,, E. J. Stratman,, Y. Li,, J. A. Fairley,, G. R. Swain,, V. A. Olson,, E. K. Sargent,, S. C. Kehl,, M. A. Frace,, R. Kline,, S. L. Foldy,, J. P. Davis,, and I. K. Damon. 2004. The detection of monkeypox in humans in the Western Hemisphere. N. Engl. J. Med. 350:342350.
74. Robinson, A. J.,, and D. J. Lyttle,. 1992. Parapoxviruses: their biology and potential as recombinant vaccine vectors, p. 285327. In M. M. Binns, and G. L. Smith (ed.), Recombinant Poxviruses. CRC Press, Inc., Boca Raton, FL.
75. Ropp, S. L.,, Q. Jin,, J. C. Knight,, R. F. Massung,, and J. J. Esposito. 1995. PCR strategy for identification and differentiation of small pox and other orthopoxviruses. J. Clin. Microbiol. 33:20692076.
76. Rouhandeh, H., 1988. Yaba virus, p. 115. In G. Darai (ed.), Virus Diseases in Laboratory and Captive Animals. Martinus Nijhoff, Boston, MA.
77. Rupprecht, C. E.,, L. Blass,, K. Smith,, L. A. Orciari,, M. Niezgoda,, S. G. Whitfield,, R. V. Gibbons,, M. Guerra,, and C. A. Hanlon. 2001. Human infection due to recombinant vacciniarabies glycoprotein virus. N. Engl. J. Med. 345:582586.
78. Scaramozzino, N.,, A. Ferrier-Rembert,, A. L. Favier,, C. Rothlisberger,, S. Richard,, J. M. Crance,, H. Meyer,, and D. Garin. 2007. Real-time PCR to identify variola virus or other human pathogenic orthopox viruses. Clin. Chem. 53:606613.
79. Schatzmayr, H. G.,, E. R. Lemos,, C. Mazur,, A. Schubach,, S. Majerowicz,, T. Rozental,, T. M. Schubach,, M. C. Bustamante,, and O. M. Barth. 2000. Detection of poxvirus in cattle associated with human cases in the State of Rio de Janeiro: preliminary report. Mem. Inst. Oswaldo Cruz 95:625627.
80. Sejvar, J. J.,, Y. Chowdary,, M. Schomogyi,, J. Stevens,, J. Patel,, K. Karem,, M. Fischer,, M. J. Kuehnert,, S. R. Zaki,, C. D. Paddock,, J. Guarner,, W. J. Shieh,, J. L. Patton,, N. Bernard,, Y. Li,, V. A. Olson,, R. L. Kline,, V. N. Loparev,, D. S. Schmid,, B. Beard,, R. R. Regnery,, and I. K. Damon. 2004. Human monkeypox infection: a family cluster in the midwestern United States. J. Infect. Dis. 190:18331840.
81. Seward, J. F.,, K. Galil,, I. Damon,, S. A. Norton,, L. Rotz,, S. Schmid,, R. Harpaz,, J. Cono,, M. Marin,, S. Hutchins,, S. S. Chaves,, and M. M. McCauley. 2004. Development and experience with an algorithm to evaluate suspected smallpox cases in the United States, 2002-2004. Clin. Infect. Dis. 39:14771483.
82. Shchelkunov, S. N.,, E. V. Gavrilova,, and I. V. Babkin. 2005. Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans. Mol. Cell. Probes 19:18.
83. Smee, D. F. 2008. Progress in the discovery of compounds inhibiting orthopoxviruses in animal models. Antivir. Chem. Chemother. 19:115124.
84. Smith, G. L.,, and M. Law. 2004. The exit of vaccinia virus from infected cells. Virus Res. 106:189197.
85. Smith, G. L.,, B. J. Murphy,, and M. Law. 2003. Vaccinia virus motility. Annu. Rev. Microbiol. 57:323342.
86. Smith, G. L.,, A. Vanderplasschen,, and M. Law. 2002. The formation and function of extracellular enveloped vaccinia virus. J. Gen. Virol. 83:29152931.
87. Stemmler, M.,, H. Neubauer,, and H. Meyer. 2001. Comparison of closely related orthopoxvirus isolates by random amplified polymorphic DNA and restriction fragment length polymorphism analysis. J. Vet. Med. B 48:647654.
88. Stich, A.,, H. Meyer,, B. Kohler,, and K. Fleischer. 2002. Tanapox: first report in a European traveller and identification by PCR. Trans. R. Soc. Trop. Med. Hyg. 96:178179.
89. Torfason, E. G.,, and S. Gunadottir. 2002. Polymerase chain reaction for laboratory diagnosis of orf virus infections. J. Clin. Virol. 24:7984.
90. Towbin, H.,, T. Staehelin,, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:43504354.
91. Trama, J. P.,, M. E. Adelson,, and E. Mordechai. 2007. Identification and genotyping of molluscum contagiosum virus from genital swab samples by real-time PCR and Pyrosequencing. J. Clin. Virol. 40:325329.
92. Trindade, G. S.,, F. G. da Fonseca,, J. T. Marques,, S. Diniz,, J. A. Leite,, S. DeBodt,, Y. Van der Peer,, C. A. Bonjardim,, P. C. Ferreira,, and E. G. Kroon. 2004. Belo Horizonte virus: a vaccinia-like virus lacking the A-type inclusion body gene isolated from infected mice. J. Gen. Virol. 85:20152021.
93. Vora, S.,, I. Damon,, V. Fulginiti,, S. G. Weber,, M. Kahana,, S. L. Stein,, S. I. Gerber,, S. Garcia-Houchins,, E. Lederman,, D. Hruby,, L. Collins,, D. Scott,, K. Thompson,, J. V. Barson,, R. Regnery,, C. Hughes,, R. S. Daum,, Y. Li,, H. Zhao,, S. Smith,, Z. Braden,, K. Karem,, V. Olson,, W. Davidson,, G. Trindade,, T. Bolken,, R. Jordan,, D. Tien,, and J. Marcinak. 2008. Severe eczema vaccinatum in a household contact of a smallpox vaccinee. Clin. Infect. Dis.46:15551561.
94. Westwood, J. C.,, P. H. Phipps,, and E. A. Boulter. 1957. The titration of vaccinia virus on the chorioallantoic membrane of the developing chick embryo. J. Hyg. Lond. 55:123139.
95.World Health Organization. 1960. Guide to the Laboratory Diagnosis of Smallpox for Smallpox Eradication Programmes. World Health Organization, Geneva, Switzerland.
96. Zimmermann, P.,, I. Thordsen,, D. Frangoulidis,, and H. Meyer. 2005. Real-time PCR assay for the detection of tanapox virus and yaba-like disease virus. J. Virol. Methods 130: 149153.


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Taxonomy of poxviruses that infect humans

Causes milker's nodule in humans.

Citation: Damon I. 2011. Poxviruses, p 1647-1658. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch105
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Diagnostic tests for poxviruses

Abbreviations: HP, histopathology; EM, electron microscopy; HA, hemagglutination; NAT, nucleic acid test. X's indicate the utility of the tests for the specified viruses.

Pock formation on CAM and tissue culture isolation are useful.

Isolation in tissue culture only; viruses do not produce pocks on CAM.

Citation: Damon I. 2011. Poxviruses, p 1647-1658. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch105
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Conventional PCR assays for orthopoxvirus detection

Amplicons are digested with specified restriction enzymes to distinguish different viruses.

To distinguish monkeypox, variola, and cowpox viruses.

Citation: Damon I. 2011. Poxviruses, p 1647-1658. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch105
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Real-time PCR assays for poxvirus detection

Orthopoxvirus assays use the target's Vaccinia-Copenhagen nomenclature. YLDV, Yaba-like disease virus; YMTV, Yaba monkey tumor virus; nt, nucleotides.

Citation: Damon I. 2011. Poxviruses, p 1647-1658. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch105

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