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Citation: Linscott A, Sharp S. .

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1. Byers, T. J.,, R. A. Akins,, B. J. Maynard,, R. A. Lefken,, and S. M. Martin. 1980. Rapid growth of Acanthamoeba in defined media; induction of encystment by glucose-acetate starvation. J. Protozool. 27:216219.
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10. Gleeson, R. M. 1990. An improved method for thick film preparation using saponin as a lysing agent. Clin. Lab. Haematol. 19:249251.
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Preservatives used in diagnostic parasitology (intestinal tract specimens)

PVA (plastic powder used as “glue” to attach stool onto the glass slide/no fixation properties per se) and Schaudinn's fixative (mercuric chloride base) are still considered to be the “gold standard” against which all other fixatives are evaluated for organism morphology after permanent staining. Additional fixatives prepared with nonmercuric chloride-based compounds are continuing to be developed and tested.

This modification uses a copper sulfate base rather than mercuric chloride.

This modification uses a zinc base rather than mercuric chloride and apparently works well with both trichrome and iron hematoxylin stains.

These modifications use a combination of ingredients (including zinc) but are prepared from proprietary formulas. The aim is to provide a fixative that can be used for the fecal concentration, permanent stained smear, and available immunoassays for and spp. Some of these fixatives are now available commercially (check suppliers such as Medical Chemical Corp. and Meridian Bioscience). Testing for and/or the / group still require fresh or frozen specimens.

This stain can be used in place of trichrome for staining fecal smears preserved with MIF, PVA, or SAF methods. It is available commercially.

EIA, enzyme immunoassay; FA, fluorescent antibody; rapid, cartridge (membrane flow/immunochromatographic method).

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Stains used for parasitic identification

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Media used for cultivation of parasites


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