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Citation: Atlas R, Snyder J. .

Key Concept Ranking

Gram-Negative Bacteria
Antimicrobial Susceptibility Testing
Bile Esculin Azide Agar
Gram-Positive Bacteria
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Nitrite and nitrate reductase activities

Courtesy of Georges Wauters and Mario Vaneechoutte.

Addition of Zn is necessary only when both broths remain colorless. It can then differentiate between situations 3 and 4.

In case of normal nitrite reductase positivity (p), as in situation 2, nitrite is still present. In the case of very strong positive nitrite reductase activity (pp), as in situation 3, all nitrite is further reduced quickly, resulting in a false-negative nitrate reductase reaction upon addition of Griess reagents to the nitrate broth. In that case, addition of Zn is needed to confirm that nitrate is no longer present.

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Dyes and pH indicators

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McFarland standards protocol

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Culture media for enrichment, isolation, and cultivation of pathogenic bacteria

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Commercial sources of chromogenic agar media for bacteria


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