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Chapter 47 : Approaches to Identification of Anaerobic Bacteria

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Abstract:

This chapter presents an adaptable approach to anaerobic bacteriology based on the resources and capabilities of laboratories. Rare urinary tract infections caused by anaerobic bacteria can be detected first by Gram staining. Newer studies using molecular methods have shown that many organisms implicated in bacterial vaginosis cannot be recovered in culture. Clostridium difficile infection is best diagnosed by toxigenic culture, with molecular detection of the toxin B gene yielding the next best results. Analysis of cellular fatty acids has been used to develop extensive databases for anaerobic identification. This method, although mostly being replaced by faster modern methods for clinical use, is still valuable for describing new species. Sequencing of genetic markers, such as portions of the 16S rRNA gene and other useful genetic elements, is the most common method used for anaerobic identification today in clinical laboratories with molecular-assessment capability. It is clear that anaerobic bacterial protocols occupy a separate and distinct place in clinical microbiology laboratories. Laboratories must determine the extent of effort that they can devote to anaerobes and then develop their processes to perform only those protocols that they can guarantee will yield reliable, timely, and accurate results.

Citation: Baron E. 2011. Approaches to Identification of Anaerobic Bacteria, p 799-802. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch47

Key Concept Ranking

Urinary Tract Infections
0.5159092
Bile Esculin Agar
0.4672553
Gram Staining
0.46050894
Clostridium difficile
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References

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1. Amess, J. A.,, W. O’Neill,, C. N. Giollariabhaigh,, and J. K. Dytrych. 2007. A six-month audit of the isolation of Fusobacterium necrophorum from patients with sore throat in a district general hospital. Br. J. Biomed. Sci. 64:6365.
2. Baron, E.J.,, and D. M. Citron. 1997. Anaerobic identification flowchart using minimal laboratory resources. Clin. Infect. Dis. 25(Suppl. 2):S143S146.
3. Baron, E. J.,, C. Strong,, M. McTeague,, M.-L. Vaisanen,, and S. M. Finegold. 1995. Survival of anaerobes in original specimens transported by overnight mail services. Clin. Infect. Dis. 20:S174S177.
4. Baselski, V. S.,, M. El-Torky,, J. J. Coalson,, and J. P. Griffin. 1992. The standardization of criteria for processing and interpreting laboratory specimens in patients with suspected ventilator-associated pneumonia. Chest 102(Suppl.): 571S579S.
5. Bliss, D. Z.,, S. Johnson,, C. R. Clabots,, K. Savik,, and D. N. Gerding. 1997. Comparison of cycloserine-cefoxitinfructose agar (CCFA) and taurocholate-CCFA for recovery of Clostridium difficile during surveillance of hospitalized patients. Diagn. Microbiol. Infect. Dis. 29:14.
6.Centers for Disease Control and Prevention. 2005. Severe Clostridium difficile-associated disease in populations previously at low risk—four states, 2005. MMWR Morb. Mortal. Wkly. Rep. 54:12011205.
7. Chen, C. H.,, M. L. Kuo,, J. F. Shih,, T. P. Chang,, and R. P. Perng. 1993. Etiologic diagnosis of pulmonary infection by ultrasonically guided percutaneous lung aspiration. Zhonghua Yi Xue Za Zhi (Taipei) 51:333339.
8. Citron, D.M., 2007. Algorithm for identification ofanaerobic bacteria, p. 377378. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. L. Landy,, and M. A. Pfaller (ed.), Manual of Clinical Microbiology, 9th ed. ASM Press, Washington, DC.
9.Clinical and Laboratory Standards Institute.2007. Methods for antimicrobial susceptibility testing of anaerobic bacteria; approved standard—seventh edition. M11-A7. Clinical and Laboratory Standards Institute, Wayne, PA.
10. Doan, N.,, A. Contreras,, J. Flynn,, J. Morrison,, and J. Slots. 1999. Proficiencies of three anaerobic culture systems for recovering periodontal pathogenic bacteria. J. Clin. Microbiol. 37:171174.
11. Dore, P.,, R. Robert,, G. Grollier,, J. Rouffineau,, H. Lanquetot,, J. M. Charriere,, and J. L. Fauchere. 1996. Incidence of anaerobes in ventilator-associated pneumonia with use of a protected specimen brush. Am. J. Respir. Crit. Care Med. 153:12921298.
12. Dowd, S. E.,, Y. Sun,, P. R. Secor,, D. D. Rhoads,, B. M. Wolcott,, G. A. James,, and R. D. Wolcott. 2008. Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing. BMC Microbiol. 8:43.
13. Downes, J.,, J. I. Mangels,, J. Holden,, M. J. Ferraro,, and E. J. Baron. 1990. Evaluation of two single-plate incubation systems and the anaerobic chamber for the cultivation of anaerobic bacteria. J. Clin. Microbiol. 28:246248.
14. Eastwood, K.,, P. Else,, A. Charlett,, and M. Wilcox. 2009. Comparison of nine commercially available Clostridium difficile toxin detection assays, a real-time PCR assay for C. difficile tcdB, and a glutamate dehydrogenase detection assay to cytotoxin testing and cytotoxigenic culture methods. J. Clin. Microbiol. 47:32113217.
15. Goldstein, E. J.,, D. M. Citron,, P. J. Goldman,, and R. J. Goldman. 2008. National hospital survey of anaerobic culture and susceptibility methods: III. Anaerobe 14:6872.
16. Hsieh, S. Y.,, C. L. Tseng,, Y. S. Lee,, A. J. Kuo,, C. F. Sun,, Y. H. Lin,, and J. K. Chen. 2008. Highly efficient classification and identification of human pathogenic bacteria by MALDI-TOF MS. Mol. Cell Proteomics 7:448456.
17. Jarvis, W. R.,, J. Schlosser,, A. A. Jarvis,, and R. Y. Chinn. 2009. National point prevalence of Clostridium difficile in US health care facility inpatients, 2008. Am. J. Infect. Control 37:263270.
18. Joniau, S.,, S. Vlaminck,, L. H. Van,, R. Kuhweide,, and C. Dick. 2005. Microbiology of sinus puncture versus middle meatal aspiration in acute bacterial maxillary sinusitis. Am. J. Rhinol. 19:135140.
19. Jousimies-Somer, H.,, P. Summanen,, D. M. Citron,, E. J. Baron,, H. Wexler,, and S. M. Finegold. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual. Star Publishing Co., Belmont, CA.
20. Keys, C. J.,, D. J. Dare,, H. Sutton,, G. Wells,, M. Lunt,, T. McKenna,, M. McDowall,, and H. N. Shah. 2004. Compilation of a MALDI-TOF mass spectral database for the rapid screening and characterisation of bacteria implicated in human infectious diseases. Infect. Genet. Evol. 4:221242.
21. Lazarovitch, T.,, S. Freimann,, G. Shapira,, and H. Blank. 2010. Decrease in anaerobe-related bacteraemias and increase in Bacteroides species isolation rate from 1998 to 2007: a retrospective study. Anaerobe 16:201205.
22. Levy, P. Y.,, P. E. Fournier,, R. Charrel,, D. Metras,, G. Habib,, and D. Raoult. 2006. Molecular analysis of pericardial fluid: a 7-year experience. Eur. Heart J. 27:19421946.
23. Lyras, D.,, J. R. O’Connor,, P. M. Howarth,, S. P. Sambol,, G. P. Carter,, T. Phumoonna,, R. Poon,, V. Adams,, G. Vedantam,, S. Johnson,, D. N. Gerding,, and J. I. Rood. 2009. Toxin B is essential for virulence of Clostridium difficile. Nature 458:11761179.
24. Mangels, J. I.,, and B. P. Douglas. 1989. Comparison of four commercial brucella agar media for growth of anaerobic organisms. J. Clin. Microbiol. 27:22682271.
25. Nerandzic, M. M.,, and C. J. Donskey. 2009. Effective and reduced-cost modified selective medium for isolation of Clostridium difficile. J. Clin. Microbiol. 47:397400.
26. Peterson, L. R.,, R. U. Manson,, S. M. Paule,, D. M. Hacek,, A. Robicsek,, R. B. Thomson, Jr.,, and K. L. Kaul. 2007. Detection of toxigenic Clostridium difficile in stool samples by real-time polymerase chain reaction for the diagnosis of C. difficile-associated diarrhea. Clin. Infect. Dis. 45:11521160.
27. Piper, K. E.,, M. J. Jacobson,, R. H. Cofield,, J. W. Sperling,, J. Sanchez-Sotelo,, D. R. Osmon,, A. McDowell,, S. Patrick,, J. M. Steckelberg,, J. N. Mandrekar,, S. M. Fernandez,, and R. Patel. 2009. Microbiologic diagnosis of prosthetic shoulder infection by use of implant sonication. J. Clin. Microbiol. 47:18781884.
28. Price, L. B.,, C. M. Liu,, J. H. Melendez,, Y. M. Frankel,, D. Engelthaler,, M. Aziz,, J. Bowers,, R. Rattray,, J. Ravel,, C. Kingsley,, P. S. Keim,, G. S. Lazarus,, and J. M. Zenilman. 2009. Community analysis of chronic wound bacteria using 16S rRNA gene-based pyrosequencing: impact of diabetes and antibiotics on chronic wound microbiota. PLoS One 4:e6462.
29. Rennie, R. P.,, C. Brosnikoff,, L. Turnbull,, L. B. Reller,, S. Mirrett,, W. Janda,, K. Ristow,, and A. Krilcich. 2008. Multicenter evaluation of the Vitek 2 anaerobe and Corynebacterium identification card. J. Clin. Microbiol. 46:26462651.
30. Rosenblatt, J. E.,, and D. R. Gustafson. 1995. Evaluation of the Etest for susceptibility testing of anaerobic bacteria. Diagn. Microbiol. Infect. Dis. 22:279284.
31. Simmon, K. E.,, S. Mirrett,, L. B. Reller,, and C. A. Petti. 2008. Genotypic diversity of anaerobic isolates from bloodstream infections. J. Clin. Microbiol. 46:15961601.
32. Sloan, L. M.,, B. J. Duresko,, D. R. Gustafson,, and J. E. Rosenblatt. 2008. Comparison of real-time PCR for detection of the tcdC gene with four toxin immunoassays and culture in diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 46:19962001.
33. Srinivasan, S.,, and D. N. Fredricks. 2008. The human vaginal bacterial biota and bacterial vaginosis. Interdiscip. Perspect. Infect. Dis. 2008:750479.
34. Stoakes, L.,, T. Kelly,, B. Schieven,, D. Harley,, M. Ramos,, R. Lannigan,, D. Groves,, and Z. Hussain. 1991. Gas-liquid chromatographic analysis of cellular fatty acids for identification of gram-negative anaerobic bacilli. J. Clin. Microbiol. 29:26362638.
35. Sturm, P. D.,, E. J. Van,, S. Veltman,, E. Meuleman,, and T. Schulin. 2006. Urosepsis with Actinobaculum schaalii and Aerococcus urinae. J. Clin. Microbiol. 44:652654.
36. Summanen, P.,, H. M. Wexler,, and S. M. Finegold. 1992. Antimicrobial susceptibility testing of Bilophila wadsworthia by using triphenyltetrazolium chloride to facilitate the endpoint determination. Antimicrob. Agents Chemother. 36:16581664.
37. Summanen, P. H.,, M. McTeague,, M. L. Vaisanen,, C. A. Strong,, and S. M. Finegold. 1999. Comparison of recovery of anaerobic bacteria using the Anoxomat, anaerobic chamber, and GasPak jar systems. Anaerobe 5:59.
38. Tuner, K.,, E. J. Baron,, P. Summanen,, and S. M. Finegold. 1992. Cellular fatty acids in Fusobacterium species as a tool for identification. J. Clin. Microbiol. 30:32253229.
39. Underdahl, J. P.,, G. J. Florakis,, R. E. Braunstein,, D. A. Johnson,, P. Cheung,, J. Briggs,, and D. M. Meisler. 2000. Propionibacterium acnes as a cause of visually significant corneal ulcers. Cornea 19:451454.
40. Van Horn, K. G.,, C. D. Audette,, D. Sebeck,, and K. A. Tucker. 2008. Comparison of the Copan ESwab system with two Amies agar swab transport systems for maintenance of microorganism viability. J. Clin. Microbiol. 46:16551658.

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