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Chapter 58 : General Approaches to Identification of , and Obligate Intracellular Bacteria

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Abstract:

This chapter summarizes the epidemiology of infections and the diagnostic tests most often used for the detection of the causative bacteria such as , , and obligate intracellular bacteria. It talks about the most frequently used tests in clinical microbiology laboratories, the Gram stain and culture on artificial media, which are unable to detect these organisms if present in clinical samples. Molecular diagnostic tools and better culture methods have significantly improved the ability to detect these agents and to diagnose the diseases that they cause. The diagnostic tests most often used for the detection of the causative bacteria are described in the chapter.

Citation: Dumler J. 2011. General Approaches to Identification of , and Obligate Intracellular Bacteria, p 964-969. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch58

Key Concept Ranking

Culture Methods
0.6880485
Tropheryma whipplei
0.625
Culture Media
0.5809328
Bacteria
0.56192183
0.6880485
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Tables

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TABLE 1

Epidemiology and clinical diseases associated with Anaplasma, Chlamydia, Coxiella, Ehrlichia, Mycoplasma, Orientia, Rickettsia, Tropheryma, and Ureaplasma infections

Citation: Dumler J. 2011. General Approaches to Identification of , and Obligate Intracellular Bacteria, p 964-969. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch58
Generic image for table
TABLE 2

Diagnostic tests for Anaplasma, Chlamydia, Coxiella, Ehrlichia, Mycoplasma, Orientia, Rickettsia, Tropheryma, and Ureaplasma

Abbreviations: CF, complement fixation; CNS, central nervous system; CSF, cerebrospinal fluid; DFA, direct fluorescent antibody; EIA, enzyme immunoassay; IFA, indirect fluorescent antibody; Ig, immunoglobulin; MIF, microimmunofluorescence; NAAT, nucleic acid amplification tests; LPS, lipopolysaccharide.

Citation: Dumler J. 2011. General Approaches to Identification of , and Obligate Intracellular Bacteria, p 964-969. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch58

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