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Chapter 70 : Phenotypic Methods for Detecting Antibacterial Resistance

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Abstract:

This chapter on special phenotypic methods for detecting antibacterial resistance describes details of tests for detection of high-level aminoglycoside resistance and acquired vancomycin resistance in enterococci; tests for detection of inducible clindamycin resistance in streptococci; tests for detection of penicillin, oxacillin, vancomycin, induc-ible clindamycin, and high-level mupirocin resistance in staphylococci; tests for detection of extended-spectrum-β-lactamase (ESBL) and carbapenemase production in ; and tests for detection of β-lactamases in multiple organisms. Other special phenotypic tests described in the chapter are those for determining the bactericidal activity or combined activities of antimicrobial agents; they are different from the tests that screen for a specific resistance mechanism. As some of the phenotypic tests described here for are not recommended for coagulase-negative staphylococci (CoNS), for detection of oxacillin resistance, the two groups are discussed separately as and and CoNS except . In the clinical laboratory, β-lactamase tests can be used for two purposes. The first is to detect an underlying mechanism of resistance that may not be detected using routine susceptibility testing methods, and the second is to detect a mechanism of resistance that is an infection control concern and epidemiologically important.

Citation: Swneson J, Patel J, Jorgensen J. 2011. Phenotypic Methods for Detecting Antibacterial Resistance, p 1155-1179. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch70

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1. Abele-Horn, M.,, L. Hommers,, R. Trabold,, and M. Frosch. 2006. Validation of VITEK 2 version 4.01 software for detection, identification, and classification of glycopeptide-resistant enterococci. J. Clin. Microbiol. 44: 71 76.
2. Ajantha, G.,, R. Kulkarni,, J. Shetty,, S. Shubhada,, and P. Jain. 2009. Phenotypic detection of inducible clindamycin resistance among Staphylococcus aureus isolates by using the lower limit of recommended inter-disk distance. Indian J. Pathol. Microbiol. 51: 376 378.
3. Aldridge, K. E.,, D. Ashcraft,, K. Cambre,, C. L. Pierson,, S. G. Jenkins,, and J. E. Rosenblatt. 2001. Multicenter survey of the changing in vitro antimicrobial susceptibilities of clinical isolates of Bacteroides fragilis group, Prevotella, Fusobacterium, Porphyromonas, and Peptostreptococcus species. Antimicrob. Agents Chemother. 45: 1238 1243.
4. Aligholi, M.,, M. Emaneini,, F. Jabalameli,, S. Shahsavan,, H. Dabiri,, and H. Sedaght. 2008. Emergence of high-level vancomycin-resistant Staphylococcus aureus in the Imam Khomeini Hospital in Tehran. Med. Princ. Pract. 17: 432 434.
5. Anderson, K. F.,, D. R. Lonsway,, J. K. Rasheed,, J. Biddle,, B. Jensen,, L. K. McDougal,, R. B. Carey,, A. Thompson,, S. Stocker,, B. Limbago,, and J. B. Patel. 2007. Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobacteriaceae. J. Clin. Microbiol. 45: 2723 2725.
6. Andes, D.,, and W. A. Craig. 2005. Treatment of infections with ESBL-producing organisms: pharmacokinetic and pharmacodynamic considerations. Clin. Microbiol. Infect. 11 (Suppl. 6): 10 17.
7. Archer, G. L.,, and M. W. Climo. 1994. Antimicrobial susceptibility of coagulase-negative staphylococci. Antimicrob. Agents Chemother. 38: 2231 2237.
8. Arias, C. A.,, and B. E. Murray. 2008. Emergence and management of drug-resistant enterococcal infections. Expert Rev. Anti-Infect. Ther. 6: 637 655.
9. Aubert, G.,, S. Passot,, F. Lucht,, and G. Dorche. 1990. Selection of vancomycin- and teicoplanin-resistant Staphylococcus haemolyticus during teicoplanin treatment of S. epidermidis infection. J. Antimicrob. Chemother. 25: 491 493.
10. Bayer, A. S.,, and J. O. Morrison. 1984. Disparity between timed-kill and checkerboard methods for determination of in vitro bactericidal interactions of vancomycin plus rifampin versus methicillin-susceptible and resistant Staphylococcusaureus. Antimicrob. Agents Chemother. 26: 220 223.
11. Bennett, K.,, and S. E. Sharp. 2008. Rapid differentiation of methicillin-resistant Staphylococcus aureus and methicillinsusceptible Staphylococcus aureus from blood cultures by use of a direct cefoxitin disk diffusion test. J. Clin. Microbiol. 46: 3836 3838.
12. Biedenbach, D. J.,, J. M. Bell,, H. S. Sader,, J. D. Turnidge,, and R. N. Jones. 2009. Activities of dalbavancin against a worldwide collection of 81,673 gram-positive bacterial isolates. Antimicrob. Agents Chemother. 53: 1260 1263.
13. Bischof, L. J.,, L. Lapsley,, K. Fontecchio,, D. Jacosalem,, C. Young,, R. Hankerd,, and D. W. Newton. 2009. Comparison of chromogenic media and BD GeneOhm MRSA PCR for the detection of methicillin resistant Staphylococcus aureus from nasal swabs. J. Clin. Microbiol. 47: 2281 2283.
14. Black, J. A.,, K. S. Thomson,, J. D. Buynak,, and J. D. Pitout. 2005. Evaluation of ?-lactamase inhibitors in disk tests for detection of plasmid-mediated AmpC β-lactamases in well-characterized clinical strains of Klebsiella spp. J. Clin. Microbiol. 43: 4168 4171.
15. Black, J. A.,, K. S. Thomson,, and J. D. D. Pitout. 2004. Use of β-lactamase inhibitors in disk tests to detect plasmid-mediated AmpC b -lactamases. J. Clin. Microbiol. 42: 2203 2206.
16. Blondeau, J. M.,, D. Vaughan,, R. Laskowski,, and S. Borsos. 2001. Susceptibility of Canadian isolates of Haemophilus influenzae, Moraxella catarrhalis and Streptococcus pneumoniae to oral antimicrobial agents. Int. J. Antimicrob. Agents 17: 457 464.
17. Boyd, D. A.,, B. M. Willey,, D. Fawcett,, N. Gillani,, and M. R. Mulvey. 2008. Molecular characterization of Enterococcus faecalis N06-0364 with low-level vancomycin resistance harbouring a novel D-Ala-D-Ser gene cluster, vanL. Antimicrob. Agents Chemother. 52: 2667 2672.
18. Bradford, P. A.,, C. Urban,, N. Mariano,, S. J. Projan,, J. J. Rahal,, and K. Bush. 1997. Imipenem resistance in Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated AmpC β-lactamase, and the loss of an outer membrane protein. Antimicrob. Agents Chemother. 41: 563 569.
19. Bratu, S.,, M. Mooty,, S. Nichani,, D. Landman,, C. Gullans,, B. Pettinato,, U. Karumudi,, P. Tolaney,, and J. Quale. 2005. Emergence of KPC-possessing Klebsiella pneumoniae in Brooklyn, New York: epidemiology and recommendations for detection. Antimicrob. Agents Chemother. 49: 3018 3020.
20. Brown, D. F.,, and E. Walpole. 2001. Evaluation of the Mastalex latex agglutination test for methicillin resistance in Staphylococcus aureus grown on different screening media. J. Antimicrob. Chemother. 47: 187 189.
21. Bush, K. 2001. New β-lactamases in gram-negative bacteria: diversity and impact on the selection of antimicrobial therapy. Clin. Infect. Dis. 32: 1085 1089.
22. Cappelletty, D. M.,, and M. J. Rybak. 1996. Comparison of methodologies for synergism testing of drug combinations against resistant strains of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 40: 677 683.
22a.. Carey, R. B.,, J. B. Patel,, S. McAllister,, A. Thompson,, P. Raney,, F. C. Tenover,, C. Ginocchio,, D. Bopp,, N. Dumas,, and D. Kohlerschmidt. 2004. Abstr. 44th Intersci. Conf. Antimicrob. Agents Chemother., abstr. D-66.
23. Cartwright, C. P.,, F. Stock,, G. A. Fahle,, and V. J. Gill. 1995. Comparison of pigment production and motility tests with PCR for reliable identification of intrinsically vancomycin- resistant enterococci. J. Clin. Microbiol. 33: 1931 1933.
24. Catlin, B. W. 1975. Iodometric detection of Haemophilus influenzae β-lactamase: rapid presumptive test for ampicillin resistance. Antimicrob. Agents Chemother. 7: 265 270.
25. Cauwelier, B.,, B. Gordts,, P. Descheemaecker,, and H. Van Landuyt. 2004. Evaluation of a disk diffusion method with cefoxitin (30 mg) for detection of methicillin-resistant Staphylococcus aureus. Eur. J. Clin. Microbiol. Infect. Dis. 23: 389 392.
26. Cavassini, M.,, A. Wenger,, K. Jaton,, D. S. Blanc,, and J. Bille. 1999. Evaluation of MRSA-Screen, a simple anti-PBP 2a slide latex agglutination kit, for rapid detection of methicillin resistance in Staphylococcus aureus. J. Clin. Microbiol. 37: 1591 1594.
27. Centers for Disease Control and Prevention. 1999. Four pediatric deaths from community-acquired methicillin-resistant Staphylococcus aureus Minnesota and North Dakota, 1997-1999. MMWR Morb. Mortal. Wkly. Rep. 48: 707710.
28. Centers for Disease Control and Prevention. 2002. Outbreak of multidrug-resistant Salmonella Newport—United States, January-April 2002. MMWR Morb. Mortal. Wkly. Rep. 51: 545548.
29. Chambers, H. F. 1997. Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clin. Microbiol. Rev. 10: 781 791.
30. Chambers, H. F.,, G. Archer,, and M. Matsuhashi. 1989. Low-level methicillin resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 33: 424 428.
31. Chambers, H. F.,, and C. J. Hackbarth,. 1992. Methicillinresistant Staphylococcus aureus: genetics and mechanisms of resistance, p. 21 35. In M. T. Cafferkey (ed.), Methicillin-Resistant Staphylococcus aureus: Clinical Management and Laboratory Aspects. Marcel Dekker, Inc., New York, NY.
32. Chen, Y.,, S. A. Marshall,, P. Winokur,, S. Coffman,, W. Wilke,, P. Murray,, C. Spiegel,, M. A. Pfaller,, G. V. Doern,, and R. N. Jones. 1998. Use of molecular and reference susceptibility testing methods in a multicenter evaluation of MicroScan dried overnight gram-positive MIC panels for detection of vancomycin and high-level aminoglycoside resistances in enterococci. J. Clin. Microbiol. 36: 2996 3001.
33. Chow, J. W.,, M. J. Zervos,, S. A. Lerner,, L. A. Thal,, S. Donabedian,, D. Jaworski,, S. Tsai,, K. Shaw,, and D. B. Clewell. 1997. A novel gentamicin resistance gene in Enterococcus. Antimicrob. Agents Chemother. 41: 511 514.
34. Clinical and Laboratory Standards Institute. 2007. Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria; Approved Standard—Sixth Edition. CLSI document M11-A7. Clinical and Laboratory Standards Institute, Wayne, PA.
35. Clinical and Laboratory Standards Institute/NCCLS. 1999. Methodology for the Serum Bactericidal Test. NCCLS document M21-A. NCCLS, Wayne, PA.
36. Clinical and Laboratory Standards Institute/NCCLS. 1999. Methods for Determining Bactericidal Activity of Antimicrobial Agents. NCCLS document M26-A. NCCLS, Wayne, PA.
37. Clinical and Laboratory Standards Institute/NCCLS. 1993. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. Approved standard M7-A3. NCCLS, Villanova, PA.
38. Clinical and Laboratory Standards Institute/NCCLS. 2009. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard—Eighth Edition. CLSI document M07-A8. Clinical and Laboratory Standards Institute, Wayne, PA.
39. Clinical and Laboratory Standards Institute/NCCLS. 2009. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard—Tenth Edition. CLSI document M02-A10. Clinical and Laboratory Standards Institute, Wayne, PA.
40. Clinical and Laboratory Standards Institute/NCCLS. 2010. Performance Standards for AntimicrobialSusceptibility Testing: Twentieth Informational Supplement. CLSI document M100-S20. Clinical and Laboratory Standards Institute, Wayne, PA.
41. Clinical and Laboratory Standards Institute/NCCLS. 2009. Performance Standards for Antimicrobial Susceptibility Testing; Nineteenth Informational Supplement. CLSI document M100-S19. Clinical and Laboratory Standards Institute, Wayne, PA.
42. Clinical and Laboratory Standards Institute/NCCLS. 1999. Performance Standards for Antimicrobial Susceptibility Testing; Ninth Informational Supplement. NCCLS document M100-S9. NCCLS, Wayne, PA.
43. Clinical and Laboratory Standards Institute/NCCLS. 2007. Performance Standards for Antimicrobial Susceptibility Testing; Seventeenth Informational Supplement. CLSI document M100-S17. Clinical and Laboratory Standards Institute, Wayne, PA.
44. Cookson, B. D. 1998. The emergence of mupirocin resistance: a challenge to infection control and antibiotic prescribing practice. J. Antimicrob. Chemother. 41: 11 18.
45. Cornaglia, G.,, and G. M. Rossolini. 2009. Forthcoming therapeutic perspectives for infections due to multidrugresistant Gram-positive pathogens. Clin. Microbiol. Infect. 15: 218 223.
46. Coudron, P. E. 2005. Inhibitor-based methods for detection of plasmid mediated AmpC β-lactamases in Klebsiella spp., Escherichia coli, and Proteus mirabilis. J. Clin. Microbiol. 43: 4163 4167.
47. Coudron, P. E.,, E. S. Moland,, and K. S. Thomson. 2000. Occurrence and detection of AmpC β-lactamases among Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis isolates at a veterans medical center. J. Clin. Microbiol. 38: 1791 1796.
48. Creagh, S.,, and B. Lucey. 2007. Interpretive guidelines for mupirocin susceptibility testing of Staphylococcus spp. Using CLSI guidelines. Br. J. Biomed. Sci. 64: 1 5.
49. DeGirolami, P. C.,, and G. Eliopoulos. 1987. Antimicrobial susceptibility tests and their role in therapeutic drug monitoring. Clin. Lab. Med. 7: 499 513.
50. De Lencastre, H.,, S. A. Figueiredo,, C. Urban,, J. Rahal,, and A. Tomasz. 1991. Multiple mechanisms of methicillin resistance and improved methods for detection in clinical isolates of Staphylococcus aureus. Antimicrob. Agents Chemother. 35: 632 639.
51. Denny, A. E.,, L. R. Peterson,, D. N. Gerding,, and W. H. Hall. 1979. Serious staphylococcal infections with strains tolerant to bactericidal antibiotics. Ann. Intern. Med. 139: 1026 1031.
52. de Oliveira, N.,, A. Cardozo,, E. de Andrade Marques,, K. dos Santos,, and M. Giambiagi-deMarval. 2007. Interpretive criteria to differentiate low- and high-level mupirocin resistance in Staphylococcus aureus. J. Med. Microbiol. 56: 937 939.
53. Desjardins, M.,, K. L. Delgaty,, K. Ramotar,, C. Seetaram,, and B. Toye. 2004. Prevalence and mechanisms of erythromycin resistance in group A and group B Streptococcus: implications for reporting susceptibility results. J. Clin. Microbiol. 42: 5620 5623.
54. Diab, M.,, M. El-Damarawy,, and M. Shemis. 2008. Rapid identification of methicillin-resistant staphylococci bacteremia among intensive care unit patients. Medscape J. Med. 10: 126.
55. Dixon, J. M.,, A. E. Lipinski,, and M. E. Graham. 1977. Detection and prevalence of pneumococci with increased resistance to penicillin. Can. Med. Assoc. J. 117: 1159 1161.
56. Doern, G. V.,, A. Brueggemann,, and G. Pierce. 1997. Assessment of the oxacillin disk screening test for determining penicillin resistance in Streptococcus pneumoniae . Eur. J. Clin. Microbiol. Infect. Dis. 16: 311 314.
57. Doern, G. V.,, A. B. Brueggemann,, G. Pierce,, H. P. Holley, Jr.,, and A. Rauch. 1997. Antibiotic resistance among clinical isolates of Haemophilus influenzae in the United States in 1994 and 1995 and detection of β-lactamase-positive strains resistant to amoxicillin-clavulanate: results of a national multicenter surveillance study. Antimicrob. Agents Chemother. 41: 292 297.
58. Doi, Y.,, and D. L. Paterson. 2007. Detection of plasmid-mediated class C β-lactamases. Int. J. Infect. Dis. 11: 191 197.
59. Doi, Y.,, B. A. Potoski,, J. M. Adams-Haduch,, H. E. Sidjabat,, A. W. Pasculle,, and D. L. Paterson. 2008. Simple disk-based method for detection of Klebsiella pneumoniae carbapenemase-type β-lactamase by use of a boronic acid compound. J. Clin. Microbiol. 46: 4083 4086.
60. Dunne, W. M., Jr.,, H. Quershi,, H. Pervez,, and D. A. Nafziger. 2001. Staphylococcus epidermidis with intermediate resistance to vancomycin: elusive phenotype or laboratory artifact? Clin. Infect. Dis. 33: 135 137.
61. Dyke, J. W., 1979. β-lactamases of Staphylococcus aureus, p. 291 310. In J. M. Hamilton-Miller (ed.), β-lactamases. Academic Press, Ltd., London, United Kingdom.
62. Eisner, A.,, G. Gorkiewicz,, G. Feierl,, E. Leitner,, J. Kofer,, H. H. Kessler,, and E. Marth. 2005. Identification of glycopeptide-resistant enterococci by VITEK 2 system and conventional and real-time polymerase chain reaction. Diagn. Microbiol. Infect. Dis. 53: 17 21.
63. Eltringham, I. 1997. Mupirocin resistance and methicillin-resistant Staphylococcus aureus (MRSA). J. Hosp. Infect. 35: 1 8.
64. Emery, C. L.,, and L. A. Weymouth. 1997. Detection and clinical significance of extended-spectrum β-lactamases in a tertiary-care medical center. J. Clin. Microbiol. 35: 2061 2067.
65. Endtz, H. P.,, N. Van Den Braak,, A. Van Belkum,, W. H. Groessens,, D. Kreft,, A. B. Stroebel,, and H. A. Verbrugh. 1998. Comparison of eight methods to detect vancomycin resistance in enterococci. J. Clin. Microbiol. 36: 592 594.
66. Entenza, J. M.,, and P. Moreillon. 2009. Tigecycline in combination with other antimicrobials: a review of in vitro, animal and case report studies. Int. J. Antimicrob. Agents 34: 8.e1 8.e9.
67. Escamilla, J. 1976. Susceptibility of Haemophilus influenzae to ampicillin as determined by use of a modified, one-minute β-lactamase test. Antimicrob. Agents Chemother. 9: 196 198.
68. Fahr, A.,, U. Eigner,, M. Armbrust,, A. Caganic,, G. Dettori,, C. Chezzi,, L. Bertoncini,, M. Benecchi,, and M. G. Menozzi. 2003. Two-center collaborative evaluation of the performance of the BD Phoenix automated microbiology system for identification and antimicrobial susceptibility testing of Enterococcus spp. and Staphylococcus spp. J. Clin. Microbiol. 41: 1135 1142.
69. Feibelkorn, K. R.,, S. A. Crawford,, M. L. McElmeel,, and J. H. Jorgensen. 2003. Practical disk diffusion method for detection of inducible clindamycin resistance in Staphylococcus aureus and coagulase-negative staphylococci. J. Clin. Microbiol. 41: 4740 4744.
70. Felmingham, D.,, and R. N. Gruneberg. 2000. The Alexander Project 1996-1997: latest susceptibility data from this international study of bacterial pathogens from community-acquired lower respiratory tract infections. J. Antimicrob. Chemother. 45: 191 203.
71. Felten, A.,, B. Grandry,, P. H. Lagrange,, and I. Casin. 2002. Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA): a disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-Screen latex agglutination test. J. Clin. Microbiol. 40: 2766 2771.
72. Fernandes, C. J.,, L. A. Fernandes,, and P. Collignon, on behalf of the Australian Group on Antimicrobial Resistance. 2005. Cefoxitin resistance as a surrogate marker for the detection of methicillin-resistant Staphylococcus aureus. J. Antimicrob. Chemother. 55: 506 510.
73. Ferreira, R. B. R.,, N. L. P. Iorio,, K. L. Malvar,, A. P. F. Nunes,, L. S. Fonseca,, C. C. R. Bastos,, and K. R. N. Santos. 2003. Coagulase-negative staphylococci: comparison of phenotypic and genotypic oxacillin susceptibility tests and evaluation of the agar screening test by using different concentrations of oxacillin. J. Clin. Microbiol. 41: 3609 3614.
74. Fines, M.,, B. Perichon,, P. Reynolds,, D. F. Sahm,, and P. Courvalin. 1999. VanE, a new type of acquired glycopeptide resistance in Enterococcus faecalis BM4405. Antimicrob. Agents Chemother. 43: 2161 2164.
75. Finks, J.,, E. Wells,, T. L. Dyke,, N. Husain,, L. Plizga,, R. Heddurshetti,, M. Wilkins,, J. Rudrik,, J. Hageman,, J. Patel,, and C. Miller. 2009. Vancomycin-resistant Staphylococcus aureus, Michigan, USA, 2007. Emerg. Infect. Dis. 15: 943 945.
76. Finlay, J. E.,, L. A. Miller,, and J. A. Poupard. 1997. Interpretive criteria for testing susceptibility of staphylococci to mupirocin. Antimicrob. Agents Chemother. 41: 1137 1139.
77. Frank, K. L.,, J. L. Del Pozo,, and R. Patel. 2008. From clinical microbiology to infection pathogenesis: how daring to be different works for Staphylococcus lugdunensis. Clin. Microbiol. Rev. 21: 111 133.
78. Free, L.,, and D. Sahm. 1995. Investigation of the reformulated Remel Synergy Quad plate for detection of high-level aminoglycoside and vancomycin resistance in enterococci. J. Clin. Microbiol. 33: 1643 1645.
79. Freeman, C.,, A. Robinson,, B. Cooper,, M. Mazens-Sullivan,, R. Quintiliani, Jr.,, and C. Nightingale. 1995. In vitro antimicrobial susceptibility of glycopeptide-resistant enterococci. Diagn. Microbiol. Infect. Dis. 21: 47 50.
80. Fuchs, P. C.,, R. N. Jones,, and A. L. Barry. 1990. Interpretive criteria for disk diffusion susceptibility testing of mupirocin, a topical antibiotic. J. Clin. Microbiol. 28: 608 609.
81. Garcia-Garrote, F.,, E. Cercenado,, and E. Bouza. 2000. Evaluation of a new system, VITEK 2, for identification and antimicrobial susceptibility testing of enterococci. J. Clin. Microbiol. 38: 2108 2111.
82. Giakkoupi, P.,, L. S. Tzouvelekis,, G. L. Daikos,, V. Miriagou,, G. Petrikkos,, N. J. Legakis,, and A. C. Vatopoulos. 2005. Discrepancies and interpretation problems in susceptibility testing of VIM-1-producing Klebsiella pneumoniae isolates. J. Clin. Microbiol. 43: 494 496.
83. Gill, V. J.,, C. B. Manning,, and C. M. Ingalls. 1981. Correlation of penicillin minimum inhibitory concentrations and penicillin zone edge appearance with staphylococcal betalactamase production. J. Clin. Microbiol. 14: 437 440.
84. Gradelski, E.,, L. Valera,, L. Aleksunes,, D. Bonner,, and J. Fung-Tomc. 2001. Correlation between genotype and phenotype categorization of staphylococci based on methicillin susceptibility and resistance. J. Clin. Microbiol. 39: 2961 2963.
85. Hackbarth, C. J.,, and H. F. Chambers. 1989. Methicillinresistant staphylococci: genetics and mechanisms of resistance. Antimicrob. Agents Chemother. 33: 991 994.
86. Handwerger, S.,, and A. Tomasz. 1985. Antibiotic tolerance among clinical isolates of bacteria. Rev. Infect. Dis. 7: 368 386.
87. Hartman, B. J.,, and A. Tomasz. 1986. Expression of methicillin resistance in heterogeneous strains of Staphylococcus aureus. Antimicrob. Agents Chemother. 29: 85 92.
88. Hindler, J., 1997. Antimicrobial susceptibility tests, p. 105 154. In H. D. Isenberg (ed.), Essential Procedures in Clinical Microbiology. American Society for Microbiology, Washington, DC.
89. Hindler, J. A.,, and C. B. Inderlied. 1985. Effect of source of Mueller-Hinton agar and resistance frequency on the detection of methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 21: 205 210.
90. Hindler, J. A.,, and D. F. Sahm. 1992. Controversies and confusion regarding antimicrobial susceptibility testing of enterococci. Antimicrob. Newsl. 8: 65 74.
91. Hindler, J. A.,, and N. L. Warner. 1984. Effect of source of Mueller-Hinton agar on detection of oxacillin resistance in Staphylococcus aureus using a screening methodology. J. Clin. Microbiol. 25: 734 735.
91a.. Hindler, J. F.,, and D. A. Bruckner. 2005. Abstr. 105th Am. Soc. Microbiol. Gen. Meet., abstr. C-325.
92. Hiramatsu, K.,, N. Aritaka,, H. Hanaki,, S. kawasaki,, Y. Hosoda,, S. Hori,, Y. Fukuchi,, and I. Kobayashi. 1997. Dissemination in Japanese hospital of strains of Staphylococcus aureus heterogeneously resistant to vancomycin. Lancet 350: 1670 1673.
93. Hirano, L.,, and A. S. Bayer. 1991. β-Lactam-β-lactamaseinhibitor combinations are active in experimental endocarditis caused by β-lactamase-producing oxacillin-resistant staphylococci. Antimicrob. Agents Chemother. 35: 685 690.
94. Horstkotte, M. A.,, J. K.-M. Knobloch,, H. Rohde,, and D. Mack. 2001. Rapid detection of methicillin resistance in coagulase-negative staphylococci by a penicillin-binding protein 2a-specific latex agglutination test. J. Clin. Microbiol. 39: 3700 3702.
95. Hussain, Z.,, L. Stoakes,, S. Garrow,, S. Longo,, V. Fitzgerald,, and R. Lannigan. 2000. Rapid detection of mecA-positive and mecA-negative coagulase-negative staphylococci by an anti-penicillin binding protein 2a slide latex agglutination test. J. Clin. Microbiol. 38: 2051 2054.
96. Hussain, Z.,, L. Stoakes,, V. Massey,, D. Diagre,, V. Fitzgerald,, S. El Sayed,, and R. Lannigan. 2000. Correlation of oxacillin MIC with mecA gene carriage in coagulase-negative staphylococci. J. Clin. Microbiol. 38: 752 754.
97. Iwen, P. C.,, D. M. Kelley,, J. Linder,, and S. H. Hinrichs. 1996. Revised approach for identification and detection of ampicillin and vancomycin resistance in Enterococcus species by using MicroScan panels. J. Clin. Microbiol. 34: 1779 1783.
98. Jacobs, M. R.,, H. J. Koornhof,, R. M. Robins-Browne,, C. M. Stevenson,, Z. A. Vermaak,, I. Freiman,, G. B. Miller,, M. A. Witcomb,, M. Isaacson,, J. I. Ward,, and R. Austrian. 1978. Emergence of multiply resistant pneumococci. N. Engl. J. Med. 299: 735 740.
99. Jacobs, M. R.,, S. K. Spangler,, and P. C. Appelbaum. 1992. β-Lactamase production and susceptibility of US and European anaerobic gram-negative bacilli to β-lactams and other agents. Eur. J. Clin. Microbiol. Infect. Dis. 11: 1081 1093.
100. Jacoby, G. A.,, and P. Han. 1996. Detection of extendedspectrum β-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli. J. Clin. Microbiol. 34: 908 911.
101. Jacoby, G. A.,, and A. A. Medeiros. 1991. More extendedspectrum β-lactamases. Antimicrob. Agents Chemother. 35: 1697 1704.
102. Jarlier, V.,, M. H. Nicolas,, G. Fournier,, and A. Philippon. 1988. Extended broad-spectrum β-lactamases conferring transferable resistance to newer β-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Rev. Infect. Dis. 10: 867 878.
103. Jett, B.,, L. Free,, and D. F. Sahm. 1996. Factors influencing the Vitek gram-positive susceptibility system’s detection of vanB-encoded vancomycin resistance among enterococci. J. Clin. Microbiol. 34: 701 706.
104. Jones, R. N.,, D. C. Edson, and CAP Microbiology Resource Committee of the College of American Pathologists. 1991. Antimicrobial susceptibility testing trends and accuracy in the United States. Arch. Pathol. Lab. Med.. 115: 429 436.
105. Jorgensen, J. H.,, S. A. Crawford,, M. L. McElmeel,, and K. R. Feibelkorn. 2004. Detection of inducible clindamycin resistance of staphylococci in correlation with performance of automated broth susceptibility testing. J. Clin. Microbiol. 42: 1800 1802.
106. Kaase, M.,, S. Lenga,, S. Friedrich,, F. Szabados,, T. Sakinc,, B. Kleine,, and S. G. Gatermann. 2008. Comparison of phenotypic methods for penicillinase detection in Staphylococcus aureus. Clin. Microbiol. Infect. 14: 614 616.
107. Kao, S. J.,, I. You,, D. B. Clewell,, S. M. Donabedian,, M. J. Zervos,, J. Petrin,, K. J. Shaw,, and J. W. Chow. 2000. Detection of the high-level aminoglycoside resistance gene aph(0 )-Ib in Enterococcus faecium. Antimicrob. Agents Chemother . 44: 2876 2879.
108. Katsanis, G. P.,, J. Spargo,, M. J. Ferraro,, L. Sutton,, and G. A. Jacoby. 1994. Detection of Klebsiella pneumoniae and Escherichia coli strains producing extended-spectrum blactamases. J. Clin. Microbiol. 32: 691 696.
109. Kim, K. S.,, and B. F. Anthony. 1981. Importance of bacterial growth phase in determining minimum bactericidal concentrations of penicillin and methicillin. Antimicrob. Agents Chemother. 19: 1075 1077.
110. Kim, S. Y.,, S. G. Hong,, E. S. Moland,, and K. S. Thomson. 2007. Convenient test using a combination of chelating agents for detection of metallo-β-lactamases in the clinical laboratory. J. Clin. Microbiol. 45: 2798 2801.
111. Knapp, C.,, and J. A. Moody,. 1992. Tests to assess bactericidal activity, p. 5.16.1 5.16.33. In H. D. Isenberg (ed.), Clinical Microbiology Procedures Handbook. American Society for Microbiology, Washington, DC.
112. Knapp, C. C.,, M. D. Ludwig,, J. A. Washington,, and H. F. Chambers. 1996. Evaluation of Vitek GPS-SA card for testing of oxacillin against borderline-susceptible staphylococci that lack mec. J. Clin. Microbiol. 34: 1603 1605.
113. Knausz, M.,, A. Ghidan,, A. Grossato,, and F. Rozgonyi. 2005. Rapid detection of methicillin resistance in teicoplaninresistant coagulase-negative staphylococci by a penicillinbinding protein 29 latex agglutination method. J. Microbiol. Methods 60: 413 416.
114. Kosowska-Shick, K.,, L. M. Ednie,, P. McGhee,, K. Smith,, C. D. Todd,, A. Wehler,, ands P. C. Appelbaum. 2008. Incidence and characteristics of vancomycin nonsusceptible strains of methicillin-resistant Staphylococcus aureus at Hershey Medical Center. Antimicrob. Agents Chemother. 52: 4510 4513.
115. Krcmery, V., Jr.,, J. Trupl,, L. Drgona,, J. Lacka,, E. Kukuckova,, and E. Oravcova. 1996. Nosocomial bacteremia due to vancomycin-resistant Staphylococcus epidermidis in four patients with cancer, neutropenia, and previous treatment with vancomycin. Eur. J. Clin. Microbiol. Infect. Dis. 15: 259 261.
116. Leclercq, R. 2002. Mechanisms of resistance to macrolides and lincosamides: nature of the resistance elements and their clinical implications. Clin. Infect. Dis. 34: 482 492.
117. Leclercq, R.,, and P. Courvalin. 1997. Resistance to glycopeptides in enterococci. Clin. Infect. Dis. 24: 545 556.
118. Leclercq, R.,, S. Dutka-Malen,, J. Duval,, and P. Courvalin. 1992. Vancomycin resistance gene vanC is specific to Enterococcus gallinarum. Antimicrob. Agents Chemother. 36: 2005 2008.
119. Lee, K.,, Y. Chong,, H. B. Shin,, Y. A. Kim,, D. Yong,, and J. H. Yum. 2001. Modified Hodge and EDTA-disk synergy tests to screen metallo-beta-lactamase-producing strains of Pseudomonas and Acinetobacter species. Clin. Microbiol. Infect. 7: 88 91.
120. Leggett, J. E.,, S. A. Wolz,, and W. A. Craig. 1989. Use of serum ultrafiltrate in the serum dilution test. J. Infect. Dis. 160: 616 623.
121. Leitch, C.,, and S. Boonlayangoor,. 1992. β-lactamase tests, p. 5.3.1 5.3.8. In H. D. Isenberg (ed.), Clinical Microbiology Procedures Handbook. American Society for Microbiology, Washington, DC.
122. Lemmen, S. W.,, S. Zolldann,, S. Klik,, R. Lutticken,, K. Kummerer,, and H. Haffner. 2004. Serum bactericidal activity of piperacillin-tazobactam against Staphylococcus aureus, piperacillin-susceptible and piperacillin-resistant Escherichia coli and Pseudomonas aeruginosa. Chemotherapy 50: 27 30.
123. Leonard, S. N.,, K. L. Rossi,, K. L. Newton,, and M. J. Rybak. 2009. Evaluation of the Etest GRD for the detection of Staphylococcus aureus with reduced susceptibility to glycopeptides. J. Antimicrob. Chemother. 63: 489 492.
124. Lewis, J. S., II,, and J. H. Jorgensen. 2005. Inducible clindamycin resistance in staphylococci: should clinicians and microbiologists be concerned? Clin. Infect. Dis. 40: 280 285.
125. Livermore, D. M. 1995. β-lactamases in laboratory and clinical resistance. Clin. Microbiol. Rev. 8: 557 584.
126. Livermore, D. M.,, and M. Yuan. 1996. Antibiotic resistance and production of extended-spectrum β-lactamases amongst Klebsiella spp. from intensive care units in Europe. J. Antimicrob. Chemother. 38: 409 424.
127. Lodise, T. P.,, N. Patel,, A. Renaud-Mutart,, E. Gorodecky,, T. R. Fritsche,, and R. N. Jones. 2008. Pharmacokinetic and pharmacodynamic profile of ceftobiprole. Diagn. Microbiol. Infect. Dis. 61: 96 102.
128. Louie, L.,, A. Majury,, J. Goodfellow,, M. Louie,, and A. E. Simor. 2001. Evaluation of a latex agglutination test (MRSA-Screen) for detection of oxacillin resistance in coagulasenegative staphylococci. J. Clin. Microbiol. 39: 4149 4151.
128a.. Madhavan, T.,, D. Sievery,, J. Rudrik,, J. Torresan,, and D. Schulman. 2005. Abstr. 43rd IDSA Annu. Meet., abstr. 1073.
129. Magnotti, L. J.,, T. J. Schroeppel,, L. P. Clement,, J. M. Swanson,, T. K. Bee,, G. O. Maish III,, G. Minard,, B. L. Zarzaur,, P. E. Fischer,, T. C. Fabian,, and M. A. Croce. 2009. Efficacy of monotherapy in the treatment of Pseudomonas ventilator-associated pneumonia in patients with trauma. J. Trauma 66: 1052 1058; discussion, 1058-1059.
130. Marlowe, E. M.,, A. Linscott,, J. M., Kanatani,, and D. A. Bruckner. 2002. Practical therapeutic applications of the Oxoid PBP29 latex agglutination test for the rapid identification of methicillin-resistant Staphylococcus aureus in blood cultures. Am. J. Clin. Pathol. 118: 287 291.
131. Martineau, F.,, F. J. Picard,, N. Lansac,, C. Ménard,, R. H. Roy,, M. Ouellette,, and M. G. Bergeron. 2000. Correlation between the resistance genotype determined by multiplex PCR assays and the antibiotic susceptibility patterns of Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob. Agents Chemother. 44: 231 238.
132. Massanari, R. M.,, M. A. Pfaller,, D. S. Wakesfield,, G. T. Hammons,, L. A. McNut,, R. F. Woolson,, and C. M. Helms. 1988. Implications of acquired oxacillin resistance in management and control of Staphylococcus aureus infections. J. Infect. Dis. 158: 701 709.
133. Mateo, M.,, J. R. Maestre,, L. Aguilar,, F. Cafini,, P. Puente,, P. Sanchez,, L. Alou,, M. J. Gimenez,, and J. Prieto. 2005. Genotypic versus phenotypic characterization, with respect to susceptibility and identification, of 17 clinical isolates of Staphylococcus lugdunensis. J. Antimicrob. Chemother. 56: 287 291.
134. McKessar, S. J.,, A. M. Berry,, J. M. Bell,, J. D. Turnidge,, and J. C. Paton. 2001. Genetic characterization of vanG, a novel vancomycin resistance locus of Enterococcus faecalis. Antimicrob. Agents Chemother. 44: 3224 3228.
135. Meyer, K. S.,, C. Urban,, J. A. Eagan,, B. J. Berger,, and J. J. Rahal. 1993. Nosocomial outbreak of Klebsiella infection resistant to late-generation cephalosporins. Ann. Intern. Med. 119: 353 358.
136. Migliavacca, R.,, J.-D. Docquier,, C. Mugnaioli,, G. Amicosante,, R. Daturi,, K. Lee,, G. M. Rossolini,, and L. Pagani. 2002. Simple microdilution test for detection of metallob- lactamase production in Pseudomonas aeruginosa . J. Clin. Microbiol. 40: 4388 4390.
137. Mimica, M. J.,, E. N. Berezin,, R. L. Carvalho,, I. M. Mimica,, L. M. J. Mimica,, M. A. Safadi,, E. Schneider,, and H. H. Caiaffa-Filho. 2007. Detection of methicillin resistance in Staphylococcus aureus isolated from pediatric patients: is the cefoxitin disk diffusion test accurate enough? Braz. J. Infect. Dis. 11: 415 417.
138. Moellering, R. C., Jr.,, O. M. Koraeniowski,, M. A. Sande,, and C. B. Wennersten. 1979. Species-specific resistance to antimicrobial synergism in Streptococcus faecium and Streptococcus faecalis. J. Infect. Dis. 140: 203 208.
139. Mohanasoundaram, K. M.,, and M. K. Lalitha. 2008. Comparison of phenotypic versus genotypic methods in the detection of methicillin resistance in Staphylococcus aureus. Indian J. Med. Res. 127: 78 84.
140. Moland, E. S.,, C. C. Sanders,, and K. S. Thomson. 1998. Can results obtained with commercially available MicroScan microdilution panels serve as an indicator of β-lactamase production among Escherichia coli and Klebsiella isolates with hidden resistance to expanded-spectrum cephalosporins and aztreonam? J. Clin. Microbiol. 36: 2575 2579.
141. Mondino, P. J.,, K. R. Dos Santos,, C. de Freire Bastos Mdo,, and M. Giambiagi-deMarval. 2003. Improvement of mupirocin E-test for susceptibility testing of Staphylococcus aureus. J. Med. Microbiol. 52: 385 387.
142. Montanari, M. P.,, O. Massidda,, M. Mingoia,, and P. E. Varalco. 1996. Borderline susceptibility to methicillin in Staphylococcus aureus: a new mechanism of resistance? Microb. Drug Resist. 2: 257 260.
143. Montanari, M. P.,, M. Mingoia,, I. Cochetti,, and P. E. Varaldo. 2003. Phenotypes and genotypes of erythromycin-resistant pneumococci in Italy. J. Clin. Microbiol. 41: 428 431.
144. Mougeot, C.,, J. Guillaumat-Taillet,, and J. M. Libert. 2001. Staphylococcus aureus: nouvelle détection de la resistance intrinseque par la methode de diffusion. Pathol. Biol. 49: 199 204.
145. Murray, B. E. 1990. The life and times of the enterococcus. Clin. Microbiol. Rev. 3: 46 65.
146. M’Zali, F. H.,, J. Heritage,, D. M. Gascoyne-Binzi,, M. Denton,, N. J. Todd,, and P. M. Hawkey. 1997. Transcontinental importation into the UK of Escherichia coli expressing a plasmid-mediated AmpC-type β-lactamase exposed during an outbreak of SHV-5 extended-spectrum β-lactamase in a Leeds hospital. J. Antimicrob. Chemother. 40: 823 831.
147. Nadjar, D.,, M. Rouveau,, C. Verdet,, J. L. Donay,, J. L. Herrmann,, P. H. Lagrange,, A. Philippon,, and G. Arlet. 2000. Outbreak of Klebsiella pneumoniae producing transferable AmpC-type β-lactamase (ACC-1) originating from Hafnia alvei. FEMS Microb. Lett. 187: 35 40.
148. Nahimana, I.,, P. Francioli,, and D. S. Blanc. 2006. Evaluation of three chromogenic media (MRSA-ID, MRSA-Select and CHROMagar MRSA) and ORSAB for surveillance cultures of methicillin-resistant Staphylococcus aureus . Clin. Microbiol. Infect. 12: 1168 1174.
149. Nakasone, I.,, T. Kinjo,, N. Yamane,, K. Kisanuki,, and C. M. Shiohira. 2007. Laboratory-based evaluation of the colorimetric VITEK-2 Compact system for species identification and of the Advanced Expert System for detection of antimicrobial resistances: VITEK-2 Compact system identification and antimicrobial susceptibility testing. Diagn. Microbiol. Infect. Dis. 58: 191 198.
150. Nasim, K.,, S. Elsayed,, J. D. D. Pitout,, J. Conly,, D. L. Church,, and D. B. Gregson. 2004. New method for laboratory detection of AmpC β-lactamases in Escherichia coli and Klebsiella pneumoniae. J. Clin. Microbiol. 42: 4799 4802.
151. Neumann, M. A.,, D. F. Sahm,, C. Thornsberry,, and J. E. McGowan, Jr. 1991. Cumitech 6A, New Developments in Antimicrobial Agent Susceptibility Testing: a Practical Guide. American Society for Microbiology, Washington, DC.
152. Neves, F. P.,, R. L. Ribeiro,, R. S. Duarte,, L. M. Teixeira,, and V. L. Merquior. 2009. Emergence of the vanA genotype among Enterococcus gallinarum isolates colonising the intestinal tract of patients in a university hospital in Rio de Janeiro, Brazil. Int. J. Antimicrob. Agents 33: 211 215.
153. Nunes, A. P.,, L. M. Teixeira,, N. L. Iorio,, C. C. Bastos,, L. de Sousa Fonseca,, T. Souto-Padron,, and K. R. dos Santos. 2006. Heterogeneous resistance to vancomycin in Staphylococcus epidermidis, Staphylococcus haemolyticus and Staphylococcus warneri clinical strains: characterisation of glycopeptide susceptibility profiles and cell wall thickening. Int. J. Antimicrob. Agents 27: 307 315.
154. O’Callaghan, C. H.,, A. Morris,, S. M. Kirby,, and A. H. Shingler. 1972. Novel method for detection of β-lactamases by using a chromogenic cephalosporin substrate. Antimicrob. Agents Chemother. 1: 283 288.
155. Okabe, T.,, K. Oana,, Y. Kawakami,, M. Yamaguchi,, Y. Takahashi,, Y. Okimura,, T. Honda,, and T. Katsuyama. 2000. Limitations of Vitek GPS-418 cards in exact detection of vancomycin-resistant enterococci with the vanB genotype. J. Clin. Microbiol. 38: 2409 2411.
156. Ostrowsky, B.,, N. C. Clark,, C. Thauvin-Eliopoulos,, L. Venkataraman,, M. Samore,, F. C. Tenover,, G. M. Eliopoulos,, R. C. Moellering, Jr.,, and H. S. Gold. 1999. A cluster of VanD vancomycin-resistant Enterococcus faecium: molecular characterization and epidemiology. J. Infect. Dis. 180: 1177 1185.
157. Palavecino, E. 2004. Community-acquired methicillin-resistant Staphylococcus aureus infections. Clin. Lab. Med. 24: 403 418.
158. Palazzo, I. C. V.,, and A. L. C. Darini. 2006. Evaluation of methods for detecting oxacillin resistance in coagulasenegative staphylococci including cefoxitin disc diffusion. FEMS Microbiol. Lett. 257: 299 305.
159. Palepou, M. F.,, A. P. Johnson,, B. D. Cookson,, H. Beattie,, A. Charlett,, and N. Woodford. 1998. Evaluation of disc diffusion and Etest for determining the susceptibility of Staphylococcus aureus to mupirocin. J. Antimicrob. Chemother. 42: 577 583.
160. Pasteran, F.,, T. Mendez,, L. Guerriero,, M. Rapoport,, and A. Corso. 2009. Sensitive screening tests for suspected class A carbapenemase production in species of Enterobacteriaceae. J. Clin. Microbiol. 47: 1631 1639.
161. Paterson, D. L.,, and R. A. Bonomo. 2005. Extendedspectrum beta-lactamases: a clinical update. Clin. Microbiol. Rev. 18: 657 686.
162. Patterson, J. E. 2000. Extended-spectrum β-lactamases. Semin. Respir. Infect. 15: 299 307.
163. Paul, M.,, and L. Leibovici. 2009. Combination antimicrobial treatment versus monotherapy: the contribution of meta-analyses. Infect. Dis. Clin. N. Am. 23: 277 293.
164. Paule, S. M.,, D. M. Hacek,, B. Kufner,, K. Truchon,, R. B. Thomson, Jr.,, K. L. Kaul,, A. Robicsek,, and L. R. Peterson. 2007. Performance of the BD GeneOhm methicillin-resistant Staphylococcus aureus test before and during high-volume clinical use. J. Clin. Microbiol. 45: 2993 2998.
165. Paule, S. M.,, M. Mehta,, D. M. Hacek,, T. M. Gonzalzles,, A. Robicsek,, and L. R. Peterson. 2009. Chromogenic media vs real-time PCR for nasal surveillance of methicillin-resistant Staphylococcus aureus: impact on detection of MRSA-positive persons. Am. J. Clin. Pathol. 131: 532 539.
166. Pefanis, A.,, C. Thauvin-Eliopoulos,, G. Eliopoulos,, and R. C. Moellering, Jr. 1993. Activity of ampicillin-sulbactam and oxacillin in experimental endocarditis caused by blactamase-hyperproducing Staphylococcus aureus. Antimicrob. Agents Chemother. 37: 507 511.
167. Perazzi, B.,, M. R. Fermepin,, A. Malimovka,, S. D. Garcia,, M. Orgambide,, C. A. Vay,, R. de Torres,, and A. M. R. Famiglietti. 2006. Accuracy of cefoxitin disk testing for characterization of oxacillin resistance mediated by penicillinbinding protein 2a in coagulase-negative staphylococci. J. Clin. Microbiol. 44: 3634 3639.
168. Perez, L.,, A. Antunes,, A. Barth,, and P. d’Azevedo. 2007. Variations of agar screen tests for detection of methicillin resistance in staphylococci: focus on cefoxitin. Eur. J. Clin. Microbiol. Infect. Dis. 26: 267 270.
169. Perichon, B.,, P. Reynolds,, and P. Courvalin. 1997. VanDtype glycopeptide-resistant Enterococcus faecium BM4339. Antimicrob. Agents Chemother. 41: 2016 2018.
170. Philippon, A.,, G. Arlet,, and G. A. Jacoby. 2002. Plasmiddetermined AmpC-type β-lactamases. Antimicrob. Agents Chemother. 46: 1 11.
171. Prakash, V.,, J. S. Lewis II,, and J. H. Jorgensen. 2008. Vancomycin MICs for methicillin-resistant Staphylococcus aureus isolates differ based upon the susceptibility test method used. Antimicrob. Agents Chemother. 52: 4528.
172. Pupin, H.,, H. Renaudin,, O. Join-Lambert,, C. Bebear,, F. Megraud,, and P. Lehours. 2007. Evaluation of moxalactam with the BD Phoenix system for detection of methicillin resistance in coagulase-negative staphylococci. J. Clin. Microbiol. 45: 2005 2008.
173. Queenan, A. M.,, and K. Bush. 2007. Carbapenemases: the versatile β-lactamases. Clin. Microbiol. Rev. 20: 440 458.
174. Ramotar, K.,, W. Woods,, L. Larocque,, and B. Toye. 2000. Comparison of phenotype methods to identify enterococci intrinsically resistant to vancomycin (vanC VRE). Diagn. Microbiol. Infect. Dis. 36: 119 124.
175. Ramotar, K.,, W. Woods,, and B. Toye. 2001. Oxacillin susceptibility testing of Staphylococcus saprophyticus using disk diffusion, agar dilution, broth microdilution, and the Vitek GPS-105 card. Diagn. Microbiol. Infect. Dis. 40: 203 205.
176. Raney, P. M.,, F. C. Tenover,, R. B. Carey,, J. E. McGowan, Jr.,, and J. B. Patel. 2006. Investigation of inducible clindamycin and telithromycin resistance in isolates of b-hemolytic streptococci. Diagn. Microbiol. Infect. Dis. 55: 213 218.
177. Reller, L. B. 1986. The serum bactericidal test. Rev. Infect. Dis. 8: 803 808.
178. Resende, C. A.,, and A. M. Figueiredo. 1997. Discrimination of methicillin-resistant Staphylococcus aureus from borderlineresistant and susceptible isolates by different methods. J. Med. Microbiol. 46: 145 149.
179. Robberts, F. J.,, P. C. Kohner,, and R. Patel. 2009. Unreliable extended-spectrum β-lactamase detection in the presence of plasmid-mediated AmpC in Escherichia coli clinical isolates. J. Clin. Microbiol. 47: 358 361.
180. Roghmann, M. 2000. Predicting methicillin resistance and the effect of inadequate empiric therapy on survival in patients with Staphylococcus aureus bacteremia. Ann. Intern. Med. 160: 1001 1004.
181. Rybak, M. J.,, S. N. Leonard,, K. L. Rossi,, C. M. Cheung,, H. S. Sader,, and R. N. Jones. 2008. Characterization of vancomycin-heteroresistant Staphylococcus aureus from the metropolitan area of Detroit, Michigan, over a 22-year period (1986 to 2007). J. Clin. Microbiol. 46: 2950 2954.
182. Sabath, L. 1977. Chemical and physical factors influencing methicillin resistance of Staphylococcus aureus and Staphylococcus epidermidis. J. Antimicrob. Chemother. 3: 47 51.
183. Saha, B.,, A. K. Singh,, A. Ghosh,, and M. Bal. 2008. Identification and characterization of a vancomycin-resistant Staphylococcus aureus isolated from Kolkata (South Asia). J. Med. Microbiol. 57: 72 79.
184. Sahm, D. F.,, S. Boonlayangoor,, P. C. Iwen,, J. L. Baade,, and G. L. Woods. 1991. Factors influencing determination of high-level aminoglycoside resistance in Enterococcus faecalis. J. Clin. Microbiol. 29: 1934 1939.
185. Sahm, D. F.,, S. Boonlayangoor,, and J. E. Schulz. 1991. Detection of high-level aminoglycoside resistance in enterococci other than Enterococcus faecalis. J. Clin. Microbiol. 29: 2595 2598.
186. Sahm, D. F.,, J. Kissinger,, M. S. Gilmore,, P. R. Murray,, R. Mulder,, J. Solliday,, and B. Clarke. 1989. In vitro susceptibility studies of vancomycin-resistant Enterococcus faecalis. Antimicrob. Agents Chemother. 33: 1588 1591.
187. Sahm, D. F.,, and L. Olsen. 1990. In vitro detection of enterococcal vancomycin resistance. Antimicrob. Agents Chemother. 34: 1846 1848.
188. Sahm, D. F.,, and C. Torres. 1988. Effects of medium and inoculum variations on screening for high-level aminoglycoside resistance in Enterococcus faecalis. J. Clin. Microbiol. 26: 250 256.
189. Sahm, D. F.,, and C. Torres. 1988. High-content aminoglycoside disks for determining aminoglycoside-penicillin synergy against Enterococcus faecalis. J. Clin. Microbiol. 26: 257 260.
190. Saiman, L. 2007. Clinical utility of synergy testing for multidrugresistant Pseudomonas aeruginosa isolated from patients with cystic fibrosis: ‘the motion for.’ Paediatr. Respir. Rev. 8: 249 255.
191. Sakoulas, G.,, P. DeGirolami,, and H. S. Gold. 2001. Methicillin-susceptible Staphylococcus aureus: believe it, or not. Ann. Intern. Med. 161: 1237 1238.
192. Sakoulas, G.,, H. S. Gold,, L. Venkataraman,, P. DeGirolami,, G. M. Eliopoulos,, and Q. Qian. 2001. Methicillinresistant Staphylococcus aureus: comparison of susceptibility testing methods and analysis of mecA-positive susceptible strains. J. Clin. Microbiol. 39: 3946 3951.
193. Sanyal, D.,, A. P. Johnson,, R. C. George,, B. D. Cookson,, and A. J. Williams. 1991. Peritonitis due to vancomycinresistant Staphylococcus epidermidis. Lancet 337: 54.
194. Schlichter, J. G.,, H. MacLean,, and A. Milzer. 1949. Effective penicillin therapy in subacute bacterial endocarditis and other chronic infections. Am. J. Med. Sci. 217: 600 608.
195. Schwalbe, R. S.,, J. T. Stapleton,, and P. H. Gilligan. 1987. Emergence of vancomycin resistance in coagulase-negative staphylococci. N. Engl. J. Med. 316: 927 931.
196. Sculier, J. P.,, and J. Klastersky. 1984. Significance of serum bactericidal activity in gram-negative bacillary bacteremia in patients with and without granulocytopenia. Am. J. Med. 76: 429 435.
197. Secchi, C.,, A. L. Antunes,, L. R. Perez,, V. V. Cantarelli,, and P. A. d’Azevedo. 2008. Identification and detection of methicillin resistance in non-epidermidis coagulase-negative staphylococci. Braz. J. Infect. Dis. 12: 316 320.
198. Shehabi, A. A.,, A. Mahafzah,, I. Baadran,, F. A. Qadar,, and N. Dajani. 2000. High incidence of Klebsiella pneumoniae clinical isolates to extended-spectrum β-lactam drugs in intensive care units. Diagn. Microbiol. Infect. Dis. 36: 53 56.
199. Sievert, D. M.,, J. T. Rudrik,, J. B. Patel,, L. C. McDonald,, M. J. Wilkins,, and J. C. Hageman. 2008. Vancomycinresistant Staphylococcus aureus in the United States, 2002-2006. Clin. Infect. Dis. 46: 668 674.
200. Silva-Carvalho, M. C.,, L. A. Teixeira,, F. A. Ferreira,, A. Ribeiro,, B. T. Ferreira-Carvalho,, and A. M. Figueiredo. 2009. Comparison of different methods for detecting methicillin resistance in MRSA isolates belonging to international lineages commonly isolated in the American continent. Microbiol. Immunol. 53: 117 122.
201. Simor, A. E.,, E. Phillips,, A. McGeer,, A. Konvalinka,, M. Loeb,, H. R. Devlin,, and A. Kiss. 2007. Randomized controlled trial of chlorhexidine gluconate for washing, intranasal mupirocin, and rifampin and doxycycline versus no treatment for the eradication of methicillin-resistant Staphylococcus aureus colonization. Clin. Infect. Dis. 44: 178 185.
202. Simor, A. E.,, T. L. Stuart,, L. Louie,, C. Watt,, M. Ofner-Agostini,, D. Gravel,, M. Mulvey,, M. Loeb,, A. McGeer,, E. Bryce,, and A. Matlow. 2007. Mupirocin-resistant, methicillin- resistant Staphylococcus aureus strains in Canadian hospitals. Antimicrob. Agents Chemother. 51: 3880 3886.
203. Skov, R. L.,, L. V. Palleson,, R. L. Poulsen,, and F. Espersen. 1999. Evaluation of a new 3-h hybridization method for detecting the mecA gene in Staphylococcus aureus and comparison with existing genotypic and phenotypic susceptibility testing methods. J. Antimicrob. Chemother. 43: 467 475.
204. Slocombe, B.,, and C. Perry. 1991. The antimicrobial activity of mupirocin an update on resistance. J. Hosp. Infect. 19( Suppl. B): 19 25.
205. Souli, M.,, P. D. Rekatsina,, Z. Chryssouli,, I. Galani,, H. Giamarellou,, and K. Kanellakopoulou. 2009. Does the activity of the combination of imipenem and colistin in vitro exceed the problem of resistance in metallo-β-lactamase-producing Klebsiella pneumoniae isolates? Antimicrob. Agents Chemother. 53: 2133 2135.
206. Srinivasan, A.,, J. D. Dick,, and T. M. Perl. 2002. Vancomycin resistance in staphylococci. Clin. Microbiol. Rev. 15: 430 438.
207. Standiford, H. D.,, J. B. deMaine,, and W. M. Kirby. 1970. Antibiotic synergism of enterococci. Arch. Intern. Med. 126: 255 259.
208. Stapleton, P. D.,, K. P. Shannon,, and G. L. French. 1999. Carbapenem resistance in Escherichia coli associated with plasmid-determined CMY-4 β-lactamase production and loss of an outer membrane protein. Antimicrob. Agents Chemother . 43: 1206 1210.
209. Stefaniuk, E.,, A. Baraniak,, M. Gniadkowski,, and K. Hryniewicz. 2003. Evaluation of the BD Phoenix automated identification and susceptibility testing system in clinical microbiology laboratory practice. Eur. J. Clin. Microbiol. Infect. Dis . 22: 479 485.
210. Stepanovic, S.,, T. Hauschild,, I. Dakic,, Z. Al-Doori,, M. Svabic-Vlahovic,, L. Ranin,, and D. Morrison. 2006. Evaluation of phenotypic and molecular methods for detection of oxacillin resistance in members of the Staphylococcus sciuri group. J. Clin. Microbiol. 44: 934 937.
211. Steward, C. D.,, P. M. Raney,, A. K. Morrell,, P. P. Williams,, L. K. McDougal,, L. Jevitt,, J. E. McGowan, Jr.,, and F. C. Tenover. 2005. Testing for inducible clindamycin resistance in erythromycin-resistant isolates of Staphylococcus aureus. J. Clin. Microbiol. 43: 1716 1721.
212. Swenson, J. M.,, K. F. Anderson,, D. R. Lonsway,, A. Thompson,, S. K. McAllister,, B. M. Limbago,, R. B. Carey,, F. C. Tenover,, and J. B. Patel. 2009. Accuracy of commercial and reference susceptibility testing methods for detecting vancomycin-intermediate Staphylococcus aureus J. Clin. Microbiol. 47: 2013 2017.
213. Swenson, J. M.,, W. B. Brasso,, M. J. Ferraro,, D. J. Hardy,, C. C. Knapp,, D. Lonsway,, S. McAllister,, L. B. Reller,, H. S. Sader,, D. Shortridge,, R. Skov,, M. P. Weinstein,, B. L. Zimmer,, and J. B. Patel. 2009. Correlation of cefoxitin MICs with the presence of mecA in Staphylococcus spp. J. Clin. Microbiol. 47: 1902 1905.
214. Swenson, J. M.,, W. B. Brasso,, M. J. Ferraro,, D. J. Hardy,, C. C. Knapp,, L. K. McDougal,, L. B. Reller,, H. S. Sader,, D. Shortridge,, R. Skov,, M. P. Weinstein,, B. L. Zimmer,, and J. B. Patel. 2007. Detection of inducible clindamycin resistance in staphylococci by broth microdilution using erythromycin-clindamycin combination wells. J. Clin. Microbiol. 45: 3954 3957.
215. Swenson, J. M.,, N. Clark,, M. J. Ferraro,, D. F. Sahm,, G. Doern,, M. A. Pfaller,, L. B. Reller,, M. P. Weinstein,, R. J. Zabransky,, and F. C. Tenover. 1994. Development of a standardized screening method for detection of vancomycinresistant enterococci. J. Clin. Microbiol. 32: 1700 1704.
216. Swenson, J. M.,, N. C. Clark,, D. F. Sahm,, M. J. Ferraro,, G. Doern,, J. Hindler,, J. H. Jorgensen,, M. A. Pfaller,, L. B. Reller,, M. P. Weinstein,, R. J. Zabransky,, and F. C. Tenover. 1995. Molecular characterization and multilaboratory evaluation of Enterococcus faecalis ATCC 51299 and quality control of screening tests for vancomycin and high-level aminoglycoside resistance in enterococci. J. Clin. Microbiol. 33: 3019 3021.
217. Swenson, J. M.,, M. J. Ferraro,, D. Sahm,, N. C. Clark,, D. Culver,, F. C. Tenover, and The National Committee for Clinical Laboratory Standards Study Group on Enterococci. 1995. Multilaboratory evaluation of screening methods for detection of high-level aminoglycoside resistance in enterococci. J. Clin. Microbiol. 33: 3008 3018.
218. Swenson, J. M.,, B. C. Hill,, and C. Thornsberry. 1989. Problems with the disk diffusion test for detection of vancomycin resistance in enterococci. J. Clin. Microbiol. 27: 2140 2142. ( Erratum, 28:403, 1990.).
219. Swenson, J. M.,, B. C. Hill,, and C. Thornsberry. 1986. Screening pneumococci for penicillin resistance. J. Clin. Microbiol. 24: 749 752.
220. Swenson, J. M.,, D. Lonsway,, S. McAllister,, A. Thompson,, L. Jevitt,, W. Zhu,, and J. B. Patel. 2007. Detection of mecAmediated resistance using reference and commercial testing methods in a collection of Staphylococcus aureus expressing borderline oxacillin MICs. Diagn. Microbiol. Infect. Dis. 58: 33 39.
221. Swenson, J. M.,, R. Skov,, and J. B. Patel. 2007. The cefoxitin disk test—what a clinical microbiologist needs to know. Clin. Microbiol. Newsl. 29: 33 40.
222. Swenson, J. M.,, J. Spargo,, F. C. Tenover,, and M. J. Ferraro. 2001. Optimal inoculation methods and quality control for the NCCLS oxacillin agar screen test for detection of oxacillin resistance in Staphylococcus aureus. J. Clin. Microbiol. 39: 3781 3784.
223. Swenson, J. M.,, F. C. Tenover, and the Cefoxitin Disk Study Group. 2005. Results of disk diffusion testing with cefoxitin correlate with presence of mecA in Staphylococcus spp. J. Clin. Microbiol. 43: 3818 3823.
224. Swenson, J. M.,, P. P. Williams,, G. Killgore,, C. M. O’Hara,, and F. C. Tenover. 2001. Performance of eight methods, including two new rapid methods, for detection of oxacillin resistance in a challenge set of Staphylococcus aureus organisms. J. Clin. Microbiol. 39: 3785 3788.
225. Swenson, J. M.,, B. Wong,, A. E. Simor,, R. B. Thomson,, M. J. Ferraro,, D. J. Hardy,, J. Hindler,, J. Jorgensen,, L. B. Reller,, M. Traczewski,, L. K. McDougal,, and J. B. Patel. 5 May 2010. A multicenter study to determine disk diffusion and broth microdilution criteria for prediction of high- and low-level mupirocin resistance in Staphylococcus aureus. J. Clin. Microbiol. doi:10.1128/JCM.00340-10.
226. Tan, T. Y.,, L. S. Y. Ng,, J. He,, T. H. Koh,, and L. Y. Hsu. 2009. Evaluation of screening methods to detect plasmidmediated AmpC in Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis. Antimicrob. Agents Chemother. 53: 146 149.
227. Taylor, P. C.,, F. D. Schoenknecht,, J. C. Sherris,, and E. C. Linner. 1983. Determination of minimum bactericidal concentrations of oxacillin for Staphylococcus aureus: influence and significance of technical factors. Antimicrob. Agents Chemother . 23: 142 150.
228. Tee, W. S.,, S. Y. Soh,, R. Lin,, and L. H. Loo. 2003. Staphylococcus lugdunensis carrying the mecA gene causes catheterassociated bloodstream infection in premature neonate. J. Clin. Microbiol. 41: 519 520.
229. Tenover, F. C.,, S. L. Emery,, C. A. Spiegel,, P. A. Bradford,, S. Eells,, A. Endimiani,, R. A. Bonomo,, and J. E. McGowan, Jr. 2009. Identification of plasmid-mediated AmpC β-lactamases in Escherichia coli, Klebsiella spp., and Proteus species can potentially improve reporting of cephalosporin susceptibility testing results. J. Clin. Microbiol. 47: 294 299.
230. Tenover, F. C.,, R. N. Jones,, J. M. Swenson,, B. Zimmer,, S. McAllister,, and J. H. Jorgensen for the NCCLS Staphylococcus Working Group. 1999. Methods for improved detection of oxacillin resistance in coagulase-negative staphylococci: results of a multicenter study. J. Clin. Microbiol. 37: 4051 4058.
231. Tenover, F. C.,, M. V. Lancaster,, B. C. Hill,, C. D. Steward,, S. A. Stocker,, G. A. Hancock,, C. M. O’Hara,, N. C. Clark,, and K. Hiramatsu. 1998. Characterization of staphylococci with reduced susceptibility to vancomycin and other glycopeptides. J. Clin. Microbiol. 36: 1020 1027.
232. Tenover, F. C.,, and R. C. Moellering, Jr. 2007. The rationale for revising the Clinical and Laboratory Standards Institute vancomycin minimal inhibitory concentration interpretive criteria for Staphylococcus aureus. Clin. Infect. Dis. 44: 1208 1215.
233. Tenover, F. C.,, J. M. Swenson,, C. M. O’Hara,, and S. A. Stocker. 1995. Ability of commercial and reference antimicrobial susceptibility testing methods to detect vancomycin resistance in enterococci. J. Clin. Microbiol. 33: 1524 1527.
234. Thauvin-Eliopoulos, E.,, L. B. Rice,, G. M. Eliopoulos,, and R. C. Moellering, Jr. 1990. Efficacy of oxacillin and ampicillin-sulbactam combination in experimental endocarditis caused by β-lactamase-hyperproducing Staphylococcus aureus. Antimicrob. Agents Chemother. 34: 728 732.
235. Tomasz, A.,, H. B. Drugeon,, H. M. de Lencastre,, D. Jabes,, L. McDougal,, and J. Bille. 1989. New mechanism for methicillin resistance in Staphylococcus aureus: clinical isolates that lack the PBP 2a gene and contain modified penicillinbinding proteins with modified penicillin-binding capacity. Antimicrob. Agents Chemother. 33: 1869 1874.
236. Tomasz, A.,, S. Nachman,, and H. Leaf. 1991. Stable classes of phenotypic expression in methicillin-resistant clinical isolates of staphylococci. Antimicrob. Agents Chemother. 35: 124 129.
237. Tonin, E.,, and A. Tomasz. 1986. β-lactam-specific resistant mutants of Staphylococcus aureus. Antimicrob. Agents Chemother. 30: 577 583.
241. Tsai, S.,, M. J. Zervos,, D. B. Clewell,, S. Donabedian,, D. F. Sahm,, and J. W. Chow. 1998. A new high-level gentamicin resistance gene, aph(20) -Id, in Enterococcus spp. Antimicrob. Agents Chemother. 42: 1229 1232.
238. Tuomanen, E.,, D. T. Durack,, and A. Tomasz. 1986. Antibiotic tolerance among clinical isolates of bacteria. Antimicrob. Agents Chemother. 30: 521 527.
239. Uh, Y.,, D. H. Shin,, I. H. Jang,, G. Y. Hwang,, M. K. Lee,, K. J. Yoon,, and H. Y. Kim. 2004. Antimicrobial susceptibility patterns and macrolide resistance genes of viridans group streptococci from blood cultures in Korea. J. Antimicrob. Chemother. 53: 1095 1097.
240. van den Braak, N.,, W. Goessens,, A. van Belkum,, H. Verbrugh,, and H. Endtz. 2001. Accuracy of the VITEK 2 system to detect glycopeptide resistance in enterococci. J. Clin. Microbiol. 39: 351 353.
241. Van Horn, K. G.,, C. A. Gedris,, K. M. Rodney,, and J. B. Mitchell. 1996. Evaluation of commercial vancomycin agar screen plates for detection of vancomycin-resistant enterococci. J. Clin. Microbiol. 34: 2042 2044.
242. Veach, L. A.,, M. A. Pfaller,, M. Barrett,, F. P. Koontz,, and R. P. Wenzel. 1990. Vancomycin resistance in Staphylococcus haemolyticus causing colonization and bloodstream infection. J. Clin. Microbiol. 28: 2064 2068.
243. Velasco, D.,, M. del Mar Tomas,, M. Cartelle,, A. Becceiro,, A. Perez,, F. Molina,, R. Moure,, R. Villanueva,, and G. Bou. 2005. Evaluation of different methods for detecting methicillin (oxacillin) resistance in Staphylococcus aureus. J. Antimicrob. Chemother. 55: 379 382.
244. Vidaillac, C.,, S. N. Leonard,, H. S. Sader,, R. N. Jones,, and M. J. Rybak. 2009. In vitro activity of ceftaroline alone and in combination against clinical isolates of resistant gram-negative pathogens, including β-lactamase-producing Enterobacteriaceae and Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 53: 2360 2366.
245. Villegas, M. V.,, K. Lolans,, A. Correa,, J. N. Kattan,, J. A. Lopez,, and J. P. Quinn. 2007. First identification of Pseudomonas aeruginosa isolates producing a KPC-type carbapenem- hydrolyzing β-lactamase. Antimicrob. Agents Chemother. 51: 1553 1555.
246. Walsh, T.,, A. Bolmstrom,, A. Qwarnstrom,, P. Ho,, M. Wootton,, R. Howe,, A. MacGowan,, and D. Diekema. 2001. Evaluation of current methods for detection of staphylococci with reduced susceptibility to glycopeptides. J. Clin. Microbiol. 39: 2439 2444.
247. Weinstein, M. P.,, C. W. Stratton,, A. Ackley,, H. B. Hawley,, P. A. Robinson,, B. D. Fisher,, D. V. Alcid,, D. S. Stevens,, and L. B. Reller. 1985. Multicenter collaborative evaluation of a standardized bactericidal test as a prognostic indicator in infective endocarditis. Am. J. Med. 78: 262 269.
248. Weinstein, M. P.,, C. W. Stratton,, H. B. Hawley,, A. Ackley,, and L. B. Reller. 1987. Multicenter collaborative evaluation of a standardized serum bactericidal test as a predictor of therapeutic efficacy in acute and chronic osteomyelitis. Am. J. Med. 83: 218 222.
249. Wiener, J.,, J. P. Quinn,, P. A. Bradford,, R. V. Goering,, C. Nathan,, K. Bush,, and R. A. Weinstein. 1999. Multiple antibiotic-resistant Klebsiella and Escherichia coli in nursing homes. JAMA 281: 517 523.
250. Willey, B. M.,, B. N. Kreiswirth,, A. E. Simor,, G. Williams,, S. R. Scriver,, A. Phillips,, and D. E. Low. 1992. Detection of vancomycin resistance in Enterococcus species. J. Clin. Microbiol. 30: 1621 1624.