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Citation: Tang Y, Crowe J. .

Key Concept Ranking

Upper Respiratory Tract Infections
Human respiratory syncytial virus
Severe Acute Respiratory Syndrome
Restriction Fragment Length Polymorphism
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Image of FIGURE 1

Schematic of RSV and hMPV genomic RNAs. The order of the genes from 3′ to 5′ is depicted. The virus-specific proteins encoded by these genes are shown.

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Image of FIGURE 2

Microscopic detection of RSV and hMPV in cell cultures. (A) RSV-infected HEp-2 cells showing cells that have fused, forming large syncytia. (B and C) hMPV infection causes a focus of infection with rounding of LLC-MK2 cells and loss of monolayer integrity. (D) Indirect immunofluorescence of an hMPV-infected LLC-MK2 centrifugation culture stained with monoclonal antibody MAb-8 ( ). Images courtesy of http://www.virology.org/hpphoto3.html (A), John Williams (B and C), and David Ferguson (D).

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Table 1

Commercial RSV and hMPV DFA reagents and their performance

NA, not applicable; ND, not done; ASR, analyte-specific reagent.

Compared to cell culture unless specifically indicated. In many cases, the culture used for comparison and validation was suboptimal, making the rapid tests artificially look better. It should be recognized that performance in each laboratory may be different.

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Table 2

Main commercially available RSV rapid antigen products and their performance characteristics

Abbreviations: NA, not applicable or not available; NP, nasopharyngeal; NW, nasal wash; CLIA, Clinical Laboratory Improvement Amendments of 1988.

Compared to cell culture unless specifically indicated.

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Table 3

Commercial NAA kits and devices for detection and identification of RSV and hMPV

Abbreviations: ASR, analyte-specific reagent; Flu, influenza virus; ND, not determined; PIV, parainfluenza virus; RhV, rhinovirus; CoV, coronavirus; AdV, adenovirus; EnV, enterovirus; TEM, target-enriched multiplexing; SARS, severe acute respiratory syndrome.

Performance varies significantly due to different standard references used for validation.

Will be available as a research use only device.


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