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Chapter 98 : Human Cytomegalovirus

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Human Cytomegalovirus, Page 1 of 2

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Abstract:

The human cytomegalovirus (CMV), formally designated human herpesvirus 5 (HHV-5) by the International Committee on Taxonomy of Viruses, is a member of the family Herpesviridae, which includes herpes simplex virus types 1 (HHV-1) and 2 (HHV-2), varicella-zoster virus (HHV-3), Epstein-Barr virus (HHV-4), and human herpesviruses 6, 7, and 8. Purified blood leukocytes are used when performing the CMV antigenemia assay, while whole blood, plasma obtained from anticoagulated whole blood, serum obtained from clotted blood, or purified peripheral blood leukocytes have all been used to quantify CMV DNA in molecular amplification assays. The antigenemia assay is relatively simple to perform and is based on immunocytochemical detection of the 65-kDa lower-matrix phosphoprotein (pp65) in the nuclei of peripheral blood leukocytes. PCR is currently the most widely used molecular method for the detection of CMV DNA and mRNAs, and the sensitivity and specificity of PCR for diagnosis of active CMV infection have been evaluated. The spin-amplification shell vial assay is based on the amplification of virus in cell cultures after low-speed centrifugation and detects viral antigens produced early in the replication of CMV, before the development of cytopathic effect (CPE). Confirmation of herpes B virus in culture can be done using monoclonal antibodies or molecular techniques. PCR for herpes B virus is generally preferred over culture for diagnostic purposes, since it is comparatively rapid, is highly sensitive and specific, and avoids the need to amplify infectious virus to high titers.

Citation: Hodinka R. 2011. Human Cytomegalovirus, p 1558-1574. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch98

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Figures

Image of FIGURE 1
FIGURE 1

Fixed hematoxylin-eosin-stained lung tissue from a patient with interstitial pneumonia. Note the numerous giant cells that possess large intranuclear inclusions surrounded by characteristically clear halos (arrows). Less pronounced granular inclusions may also be present in the cytoplasm (inset). (Courtesy of Eduardo Ruchelli.)

Citation: Hodinka R. 2011. Human Cytomegalovirus, p 1558-1574. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch98
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Image of FIGURE 2
FIGURE 2

CMV antigen-positive polymorphonuclear leukocytes. Note the nuclear staining when a monoclonal antibody directed against pp65 is used. (A) Magnification, x100; (B) magnification, x400.

Citation: Hodinka R. 2011. Human Cytomegalovirus, p 1558-1574. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch98
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Image of FIGURE 3
FIGURE 3

CPE produced by a CMV isolate in human skin fibroblasts 10 days postinoculation. Unstained preparation; magnification, x100. (Courtesy of Sergio Stagno.)

Citation: Hodinka R. 2011. Human Cytomegalovirus, p 1558-1574. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch98
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Image of FIGURE 4
FIGURE 4

Demonstration of CMV early antigens in the nuclei (arrows) of infected MRC-5 cells following shell vial culture and IFA staining. (A) Staining of immediate-early antigen appears as an even matte green fluorescence with specks of brighter green. (B) Viral inclusions (owl's eyes) may be visible in the nuclei. Magnification, x400.

Citation: Hodinka R. 2011. Human Cytomegalovirus, p 1558-1574. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch98
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References

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1. Abbate, I.,, N. Finnstrom,, S. Zaniratti,, M. C. Solmone,, S. Selvaggini,, E. Bennici,, S. Neri,, C. Brega,, M. Paterno,, and M. R. Capobianchi. 2008. Evaluation of an automated extraction system in combination with Affigene CMV Trender for CMV DNA quantitative determination: comparison with nested PCR and pp65 antigen test. J. Virol. Methods 151:6165.
2. Adler, S. P.,, and J. B. Wang. 1996. Salivary antibodies to cytomegalovirus (CMV) glycoprotein B accurately predict CMV infections among preschool children. J. Clin. Microbiol. 34:26322634.
3. Allice, T.,, F. Cerutti,, F. Pittaluga,, S. Varetto,, A. Franchello,, M. Salizzoni,, and V. Ghisetti. 2008. Evaluation of a novel real-time PCR system for cytomegalovirus DNA quantitation on whole blood and correlation with pp65-antigen test in guiding pre-emptive antiviral treatment. J. Virol. Methods 148:916.
4. Arav-Boger, R.,, and R. Pass. 2007. Viral load in congenital cytomegalovirus infection. Herpes 14:1722.
5. Arens, M. Q.,, and E. M. Swierkosz,. 2007. Susceptibility test methods, p. 17051718. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. L. Landry,, and M. A. Pfaller (ed.), Manual of Clinical Microbiology, 9th ed. ASM Press, Washington, DC.
6. Baldanti, F.,, D. Lilleri,, and G. Gerna. 2008. Human cytomegalovirus load measurement and its applications for preemptive therapy in patients undergoing hematopoietic stem cell transplantation. Hematol. Oncol. 26:123130.
7. Baldanti, F.,, D. Lilleri,, and G. Gerna. 2008. Monitoring human cytomegalovirus infection in transplant recipients. J. Clin. Virol. 41:237241.
8. Baldanti, F.,, A. Sarasini,, E. Silini,, M. Barbi,, A. Lazzarin,, K. K. Biron,, and G. Gerna. 1995. Four dually resistant human cytomegalovirus strains from AIDS patients: single mutations in UL97 and UL54 open reading frames are responsible for ganciclovir-and foscarnet-specific resistance, respectively. Scand. J. Infect. Dis. Suppl. 99:103104.
9. Barbi, M.,, S. Binda,, and S. Caroppo. 2006. Diagnosis of congenital CMV infection via dried blood spots. Rev. Med. Virol. 16:385392.
10. Bestetti, A.,, C. Pierotti,, M. Terreni,, A. Zappa,, L. Vago,, A. Lazzarin,, and P. Cinque. 2001. Comparison of three nucleic acid amplification assays of cerebrospinal fluid for diagnosis of cytomegalovirus encephalitis. J. Clin. Microbiol. 39:11481151.
11. Bitsch, A.,, H. Kirchner,, R. Dennin,, J. Hoyer,, L. Fricke,, J. Steinhoff,, K. Sack,, and G. Bein. 1993. Long persistence of CMV DNA in the blood of renal transplant patients after recovery from CMV infection. Transplantation 56:108113.
12. Bitsch, A.,, H. Kirchner,, R. Dupke,, and G. Bein. 1992. Cytomegalovirus transcripts in peripheral blood leukocytes of actively infected transplant patients detected by reverse transcription-polymerase chain reaction. J. Infect. Dis. 167:740743.
13. Blank, B. S.,, P. L. Meenhorst,, J. W. Mulder,, G. J. Weverling,, H. Putter,, W. Pauw,, W. C. van Dijk,, P. Smits,, S. Lie-A-Ling,, P. Reiss,, and J. M. Lange. 2000. Value of different assays for detection of human cytomegalovirus (HCMV) in predicting the development of HCMV disease in human immunodeficiency virus-infected patients. J. Clin. Microbiol. 38:563569.
14. Boeckh, M.,, and G. Boivin. 1998. Quantitation of cytomegalovirus: methodologic aspects and clinical applications. Clin. Microbiol. Rev. 11:533554.
15. Boeckh, M. P.,, P. M., Woogerd,, T. Stevens-Ayers,, C. G. Ray,, and R. A. Bowden. 1994. Factors influencing detection of quantitative cytomegalovirus antigenemia. J. Clin. Microbiol. 32:832834.
16. Boivin, G.,, R. Belanger,, R. Delage,, C. Beliveau,, C. Demers,, N. Goyette,, and J. Roy. 2000. Quantitative analysis of cytomegalovirus (CMV) viremia using the pp65 antigenemia assay and the COBAS AMPLICOR CMV MONITOR PCR Test after blood and marrow allogeneic transplantation. J. Clin. Microbiol. 38:43564360.
17. Boppana, S. B.,, K. B. Fowler,, R. F. Pass,, L. B. Rivera,, R. D. Bradford,, F. D. Lakeman,, and W. J. Britt. 2005. Congenital cytomegalovirus infection: association between virus burden in infancy and hearing loss. J. Pediatr. 146:817823.
18. Bradford, R. D.,, G. Cloud,, A. D. Lakeman,, S. Boppana,, D. W. Kimberlin,, R. Jacobs,, G. Demmler,, P. Sanchez,, W. Britt,, S.-J. Soong,, R. J. Whitley, and the National Institute of Allergy and Infectious Diseases Collaborative Antiviral Study Group. 2005. Detection of cytomegalovirus (CMV) DNA by polymerase chain reaction is associated with hearing loss in newborns with symptomatic congenital CMV infection involving the central nervous system. J. Infect. Dis. 191:227233.
19. Burbelo, P. D.,, A. T. Issa,, K. H. Ching,, M. Exner,, W. L. Drew,, H. J. Alter,, and M. J. Iadarola. 2009. Highly quantitative serological detection of anti-cytomegalovirus (CMV) antibody. Virol. J. 6:45. doi:10.1186/1743-422X-6-45.
20. Caliendo, A. M.,, J. Ingersoll,, A. M. Fox-Canale,, S. Pargman,, T. Bythwood,, M. K. Hayden,, J. W. Bremer,, and N. S. Lurain. 2007. Evaluation of real-time PCR laboratory- developed tests using analyte specific reagents for cytomegalovirus quantification. J. Clin. Microbiol. 45:17231727.
21. Caliendo, A. M.,, B. Schuurman,, B. Yen-Lieberman,, S. A. Spector,, J. Andersen,, R. Manjiry,, C. Crumpacker,, N. S. Lurain,, and A. Erice for the CMV Working Group of the Complications of HIV Disease RAC,AIDS Clinical Trials Group. 2001. Comparison of quantitative and qualitative PCR assays for cytomegalovirus DNA in plasma. J. Clin. Microbiol. 39:13341338.
22. Caliendo, A. M.,, M. D. Shahbazian,, C. Schaper,, J. Ingersoll,, D. Abdul-Ali,, J. Boonyaratanakornkit,, X.-L. Pang,, J. Fox,, J. Preiksaitis,, and E. R. Schonbrunner. 2009. A commutable cytomegalovirus calibrator is required to improve the agreement of viral load values between laboratories. Clin. Chem. 55:9. doi:10.1373/clinchem.2009.124743.
23. Caliendo, A. M.,, K. St. George,, J. Allega,, A. C. Bullotta,, L. Gilbane,, and C. R. Rinaldo. 2002. Distinguishing cytomegalovirus (CMV) infection and disease with CMV nucleic acid assays. J. Clin. Microbiol. 40:15811586.
24. Cariani, E.,, C. P. Pollara,, B. Valloncini,, F. Perandin,, C. Bonfanti,, and N. Manca. 2007. Relationship between pp65 antigenemia levels and real-time quantitative DNA PCR for human cytomegalovirus (HCMV) management in immunocompromised patients. BMC Infect. Dis. 7:138. doi:10.1186/1471-23334-7-138.
25. Chou, S.,, S. Guentzel,, K. R. Michels,, R. C. Miner,, and W. L. Drew. 1995. Frequency of UL97 phosphotransferase mutations related to ganciclovir resistance in clinical cytomegalovirus isolates. J. Infect. Dis. 172:239242.
26. Cortez, K. J.,, S. H. Fischer,, G. A. Fahle,, L. B. Calhoun,, R. W. Childs,, A. J. Barrett,, and J. E. Bennett. 2003. Clinical trial of quantitative real-time polymerase chain reaction for detection of cytomegalovirus in peripheral blood of allogeneic hematopoietic stem-cell transplant recipients. J. Infect. Dis. 188:967972.
27. Cremer, N. E.,, M. Hoffman,, and E. H. Lennette. 1978. Role of rheumatoid factor in complement fixation and indirect hemagglutination tests for immunoglobulin M antibody to cytomegalovirus. J. Clin. Microbiol. 8:160165.
28. Curdt, I.,, G. Praast,, E. Sickinger,, J. Schultess,, I. Herold,, H. B. Braun,, S. Bernhardt,, G. T. Maine,, D. D. Smith,, S. Hsu,, H. M. Christ,, D. Pucci,, M. Hausmann,, and J. Herzogenrath. 2009. Development of fully automated determination of marker specific immunoglobulin G (IgG) avidity based on the avidity competition assay format: application for Abbott Architect CMV and Toxo IgG avidity assays. J. Clin. Microbiol. 47:603613.
29. Demmler, G. J.,, G. J. Buffone,, C. M. Schimbor,, and R. A. May. 1988. Detection of cytomegalovirus in urine from newborns by using polymerase chain reaction DNA amplification. J. Infect. Dis. 158:11771184.
30. Drouet, E.,, R. Colimon,, S. Michelson,, N. Fourcade,, A. Niveleau,, C. Ducerf,, A. Boibieux,, M. Chevallier,, and G. Denoyel. 1995. Monitoring levels of human cytomegalovirus DNA in blood after liver transplantation. J. Clin. Microbiol. 33:389394.
31. Erice, A. 1999. Resistance of human cytomegalovirus to antiviral drugs. Clin. Microbiol. Rev. 12:286297.
32. Espy, M. J.,, A. D. Wold,, D. M. Ilstrup,, and T. F. Smith. 1988. Effect of treatment of shell vial cell cultures with dimethyl sulfoxide and dexamethasone for detection of cytomegalovirus. J. Clin. Microbiol. 26:10911093.
33. Fedorko, D. P.,, D. M. Ilstrup,, and T. F. Smith. 1989. Effect of age of shell vial monolayers on detection of cytomegalovirus from urine specimens. J. Clin. Microbiol. 27:21072109.
34. Fox, J. C.,, P. D. Griffiths,, and V. C. Emery. 1992. Quantification of human cytomegalovirus DNA using the polymerase chain reaction. J. Gen. Virol. 73:24052408.
35. Garrigue, I.,, S. Boucher,, L. Couzi,, A. Caumont,, C. Dromer,, M. Neau-Cransac,, R. Tabrizi,, M.-H. Schrive,, H. Fleury,, and M.-E. Lafon. 2006. Whole blood real-time quantitative PCR for cytomegalovirus infection follow-up in transplant recipients. J. Clin. Virol. 36:7275.
36. Gault, E., Y. Michael,, A. Dehee,, C. Belabani,, J. C. Nicolas,, and A. Garbarg-Chenon. 2001. Quantitation of human cytomegalovirus DNA by real-time PCR. J. Clin. Microbiol. 39:772775.
37. Gerdes, J. C.,, E. K. Spees,, K. Fitting,, J. Hiraki,, M. Sheehan,, D. Duda,, T. Jarvi,, C. Roehl,, and A. D. Robertson. 1993. Prospective study utilizing a quantitative polymerase chain reaction for detection of cytomegalovirus DNA in the blood of renal transplant patients. Transplant. Proc. 25:14111413.
38. Gerna, G.,, M. Furione,, F. Baldanti,, and A. Sarasini. 1994. Comparative quantitation of human cytomegalovirus DNA in blood leukocytes and plasma of transplant and AIDS patients. J. Clin. Microbiol. 32:27092717.
39. Gerna, G.,, and D. Lilleri. 2006. Monitoring transplant patients for human cytomegalovirus: diagnostic update. Herpes 13:411.
40. Gerna, G.,, E. Percivalle,, M. Torsellini,, and M. G. Revello. 1998. Standardization of the human cytomegalovirus antigenemia assay by means of in vitro-generated pp65-positive peripheral blood polymorphonuclear leukocytes. J. Clin. Microbiol. 36:35853589.
41. Gerna, G.,, M. G. Revello,, E. Percivalle,, M. Zavattoni,, M. Parea,, and M. Battaglia. 1990. Quantitation of human cytomegalovirus viremia by using monoclonal antibodies to different viral proteins. J. Clin. Microbiol. 28:26812688.
42. Gerna, G.,, M. Zavattoni,, F. Baldanti,, M. Furione,, L. Chezzi,, M. G. Revello,, and E. Percivalle. 1998. Circulating cytomegalic endothelial cells are associated with high human cytomegalovirus (HCMV) load in AIDS patients with latestage disseminated HCMV disease. J. Med. Virol. 55:6474.
43. Gerna, G.,, D. Zipeto,, M. Parea,, E. Percivalle,, M. Zavattoni,, A. Gaballo,, and G. Milanesi. 1991. Early virus isolation, early structural antigen detection and DNA amplification by the polymerase chain reaction in polymorphonuclear leukocytes from AIDS patients with human cytomegalovirus viraemia. Mol. Cell. Probes 5:365374.
44. Gerna, G.,, D. Zipeto,, M. Parea,, M. G. Revello,, E. Silini,, E. Percivalle,, M. Zavattoni,, P. Grossi,, and G. Milanesi. 1991. Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia, and DNAemia. J. Infect. Dis. 164:488498.
45. Gimeno, C.,, C. Solano,, J. C. Latorre,, J. C. Hernandez-Boluda,, M. A. Clari,, M. J. Remigia,, S. Furio,, M. Calabuig,, N. Tormo,, and D. Navarro. 2008. Quantification of DNA in plasma by an automated real-time PCR assay (cytomegalovirus PCR kit) for surveillance of active cytomegalovirus infection and guidance of preemptive therapy for allogeneic hematopoietic stem cell transplant recipients. J. Clin. Microbiol. 46:33113318.
46. Gleaves, C. A.,, D. A. Hursh,, and J. D. Meyers. 1992. Detection of human cytomegalovirus in clinical specimens by centrifugation culture with a nonhuman cell line. J. Clin. Microbiol. 30:10451048.
47. Gleaves, C. A.,, T. F. Smith,, E. A. Shuster,, and G. R. Pearson. 1984. Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen. J. Clin. Microbiol. 19:917919.
48. Goegebuer, T.,, B. Van Meensel,, K. Beuselinck,, V. Cossey,, M. Van Ranst,, M. Hanssens,, and K. Lagrou. 2009. Clinical predictive value of real-time PCR quantification of human cytomegalovirus DNA in amniotic fluid samples. J. Clin. Microbiol. 47:660665.
49. Gouarin, S.,, A. Vabret,, C. Scieux,, F. Agbalika,, J. Cherot,, C. Mengelle,, C. Deback,, J. Petitjean,, J. Dina,, and F. Freymuth. 2007. Multicentric evaluation of a new commercial cytomegalovirus real-time PCR quantitation assay. J. Virol. Methods 146:147154.
50. Gourlain, K.,, D. Salmon,, E. Gault,, C. Leport,, C. Katlama,, S. Matheron,, D. Costagliola,, M.-C. Mazeron,, A.-M. Fillet, and the Predivir Study Group and CMV AC11 Study Group. 2003. Quantitation of cytomegalovirus (CMV) DNA by real-time PCR for occurrence of CMV disease in HIV-infected patients receiving highly active antiretroviral therapy. J. Med. Virol. 69:401407.
51. Gozlan, J.,, J.-M. Saloed,, C. Chouaid,, C. Duvivier,, O. Picard,, M.-C. Meyohas,, and J.-C. Petit. 1993. Human cytomegalovirus (HCMV) late-mRNA detection in peripheral blood of AIDS patients: diagnostic value for HCMV disease compared with those of viral culture and HCMV DNA detection. J. Clin. Microbiol. 31:19431945.
52. Grangeot-Keros, L.,, M. J. Mayaux,, P. Lebon,, F. Freymuth,, G. Eugene,, R. Stricker,, and E. Dussaix. 1997. Value of cytomegalovirus (CMV) IgG avidity index for the diagnosis of primary CMV infection in pregnant women. J. Infect. Dis. 175:944946.
53. Griscelli, F.,, M. Barrois,, S. Chauvin,, S. Lastere,, D. Bellet,, and J.-H. Bourhis. 2001. Quantification of human cytomegalovirus DNA in bone marrow transplant recipients by real-time PCR. J. Clin. Microbiol. 39:43624369.
54. Guiver, M.,, A. J. Fox,, K. Mutton,, N. Mogulkoc,, and J. Egan. 2001. Evaluation of CMV viral load using TaqMan CMV quantitative PCR and comparison with CMV antigenemia in heart and lung transplant recipients. Transplantation 71:16091615.
55. Hanson, K. E.,, L. B. Reller,, J. Kurtzberg,, M. Horwitz,, G. Long,, and B. D. Alexander. 2007. Comparison of the Digen Hybrid Capture System Cytomegalovirus (CMV) DNA (Version 2.0), Roche CMV UL54 analyte-specific reagent, and QIAGEN RealArt CMV LightCycler PCR reagent tests using AcroMetrix OptiQuant CMV DNA quantification panels and specimens from allogeneic-stem-cell transplant recipients. J. Clin. Microbiol. 45:19721973.
56. Ho, S. K. N.,, C.-Y. Lo,, I. K. P. Cheng,, and T.-M. Chan. 1998. Rapid cytomegalovirus pp65 antigenemia assay by direct erythrocyte lysis and immunofluorescence staining. J. Clin. Microbiol. 36:638640.
57. Hodinka, R. L., 1999. Serological tests in clinical virology, p. 195211. In E. H. Lennette, and T. F. Smith (ed.), Laboratory Diagnosis of Viral Infections, 3rd ed. Marcel Dekker, New York, NY.
58. Hodinka, R. L. 1998. The clinical utility of viral quantitation using molecular methods. Clin. Diagn. Virol. 10:2547.
59. Hsia, K.,, D. H. Spector,, J. Lawrie,, and S. A. Spector. 1989. Enzymatic amplification of human cytomegalovirus sequences by polymerase chain reaction. J. Clin. Microbiol. 27:18021809.
60. Husain, S.,, C. E. Pietrangeli,, and A. Zeevi. 2009. Delayed onset CMV disease in solid organ transplant recipients. Transplant Immunol. 21:19. doi:10.1016/j.trim.2008.12.004.
61. Jang, E. Y.,, S. Y. Park,, E. J. Lee,, E. H. Song,, Y. P. Chong,, S.-O. Lee,, S.-O. Choi,, J. H. Woo,, Y. S. Kim,, and S.-H. Kim. 2009. Diagnostic performance of the cytomegalovirus (CMV) antigenemia assay in patients with CMV gastrointestinal disease. Clin. Infect. Dis. 48:e121e124.
62. Koidl, C.,, M. Bozic,, E. Marth,, and H. H. Kessler. 2008. Detection of CMV DNA: is EDTA whole blood superior to EDTA plasma? J. Virol. Methods 154:210212.
63. Lam, K. M.,, N. Oldenburg,, M. A. Khan,, V. Gaylore,, G. W. Mikhail,, P. D. Strouhal,, J. M. Middeldorp,, N. Banner,, and M. Yacoub. 1998. Significance of reverse transcription polymerase chain reaction in the detection of human cytomegalovirus gene transcripts in thoracic organ transplant recipients. J. Heart Lung Transplant. 17:555565.
64. Landini, M. P.,, and T. Lazzarotto. 1999. Prenatal diagnosis of congenital cytomegalovirus infection: light and shade. Herpes 6:4549.
65. Landini, M. P.,, T. Lazzarotto,, G. T. Maine,, A. Ripalti,, and R. Flanders. 1995. Recombinant mono- and polyantigens to detect cytomegalovirus-specific immunoglobulin M in human sera by enzyme immunoassay. J. Clin. Microbiol. 33:25352542.
66. Landry, M. L.,, and D. Ferguson. 2000. Reduced ability to culture cytomegalovirus from peripheral blood leukocytes isolated by direct erythrocyte lysis. J. Clin. Microbiol. 38:3906.
67. Landry, M. L.,, and D. Ferguson. 2000. Two-hour cytomegalovirus pp65 antigenemia assay for rapid quantitation of cytomegalovirus in blood samples. J. Clin. Microbiol. 38:427428.
68. Landry, M. L.,, and D. Ferguson. 1993. Comparison of quantitative cytomegalovirus antigenemia assay with culture methods and correlation with clinical disease. J. Clin. Microbiol. 31:28512856.
69. Landry, M. L.,, D. Ferguson,, S. Cohen,, K. Huber,, and P. Wetherill. 1995. Effect of delayed specimen processing on cytomegalovirus antigenemia test results. J. Clin. Microbiol. 33:257259.
70. Landry, M. L.,, D. Ferguson,, T. Stevens-Ayers,, M. W. A. de Jonge,, and M. Boeckh. 1996. Evaluation of CMV Brite kit for detection of cytomegalovirus pp65 antigenemia in peripheral blood leukocytes by immunofluorescence. J. Clin. Microbiol. 34:13371339.
71. Lazzarotto, T.,, B. Guerra,, M. Lanari,, L. Gabrielli,, M. P. Landini. 2008. New advances in the diagnosis of congenital cytomegalovirus infection. J. Clin. Virol. 41:192197.
72. Lazzarotto, T.,, P. Spezzacatena,, P. Pradelli,, D. A. Abate,, S. Varani,, and M. P. Landini. 1997. Avidity of immunoglobulin G directed against human cytomegalovirus during primary and secondary infections in immunocompetent and immunocompromised subjects. Clin. Diagn. Lab. Immunol. 4:469473.
73. Lazzarotto, T.,, S. Varani,, P. Spezzacatena,, P. Pradelli,, L. Potena,, A. Lombardi,, V. Ghisetti,, L. Gabrielli,, D. A. Abate,, C. Magelli,, and M. P. Landini. 1998. Delayed acquisition of high-avidity anti-cytomegalovirus antibody is correlated with prolonged antigenemia in solid organ transplant recipients. J. Infect. Dis. 178:11451149.
74. Leruez-Ville, M.,, M. Ouachee,, R. Delarue,, A.-S. Sauget,, S. Blanche,, A. Buzyn,, and C. Rouzioux. 2003. Monitoring cytomegalovirus infection in adult and pediatric bone marrow transplant recipients by a real-time PCR assay performed with blood plasma. J. Clin. Microbiol. 41:20402026.
75. Machida, U.,, M. Kami,, T. Fukui,, Y. Kazuyama,, M. Kinoshita,, Y. Tanaka,, Y. Kanda,, S. Ogawa,, H. Honda,, S. Chiba,, K. Mitani,, Y. Muto,, K. Osumi,, S. Kimura,, and H. Hirai. 2000. Real-time automated PCR for early diagnosis and monitoring of cytomegalovirus infection after bone marrow transplantation. J. Clin. Microbiol. 38:25362542.
76. MacKenzie, D.,, and L. C. McLaren. 1989. Increased sensitivity for rapid detection of cytomegalovirus by shell vial centrifugation assay using mink lung cell cultures. J. Virol. Methods 26:183188.
77. May, G.,, J. E. Kuhn,, and B. R. Eing. 2006. Comparison of two commercially available pp65 antigenemia tests and COBAS Amplicor CMV Monitor for early detection and quantification of episodes of human CMV-viremia in transplant recipients. Intervirology 49:261265.
78. Mayo, D. R.,, T. Brennan,, S. P. Sirpenski,, and C. Seymour. 1985. Cytomegalovirus antibody detection by three commercially available assays and complement fixation. Diagn. Microbiol. Infect. Dis. 3:455459.
79. McHugh, T. M.,, C. H. Casavant,, J. C. Wilber,, and D. P. Stites. 1985. Comparison of six methods for the detection of antibody to cytomegalovirus. J. Clin. Microbiol. 22:10141019.
80. Mengelle, C.,, C. Pasquier,, L. Rostaing,, K. Sandres-Saune,, J. Puel,, L. Berges,, L. Righi,, C. Bouquies,, and J. Izopet. 2003. Quantitation of human cytomegalovirus in recipients of solid organ transplants by real-time quantitative PCR and pp65 antigenemia. J. Med. Virol. 69:225231.
81. Mengelle, C.,, K. Sandres-Saune,, C. Pasquier,, L. Rostaing,, J.-M. Mansuy,, M. Marty,, I. Da Silva,, M. Attal,, P. Massip,, and J. Izopet. 2003. Automated extraction and quantitation of human cytomegalovirus DNA in whole blood by real-time PCR assay. J. Clin. Microbiol. 41:38403845.
82. Mengoli, C.,, R. Cusinato,, M. A. Biasolo,, S. Cesaro,, C. Parolin,, and G. Palu. 2004. Assessment of CMV load in solid organ trnasplant recipients by pp65 antigenemia and real-time quantitative DNA PCR assay: correlation with pp67 RNA detection. J. Med. Virol. 74:7884.
83. Michelin, B. D. A.,, I. Hadzisejdic,, M. Bozic,, M. Grahovac,, M. Hess,, B. Grahovac,, E. Marth,, and H. H. Kessler. 2008. Detection of cytomegalovirus (CMV) DNA in EDTA wholeblood samples: evaluation of the quantitative artus CMV LightCycler PCR kit in conjunction with automated sample preparation. J. Clin. Microbiol. 46:12411245.
84. Nelson, P. N.,, B. K. Rawal,, Y. S. Boriskin,, K. E. Mathers,, R. L. Powles,, H. M. Steel,, Y. S. Tryhorn,, P. D. Butcher,, and J. C. Booth. 1996. A polymerase chain reaction to detect a spliced late transcript of human cytomegalovirus in the blood of bone marrow transplant recipients. J. Virol. Methods 56:139148.
85. Novak, Z.,, S. A. Ross,, R. K. Patro,, S. K. Pati,, M. K. Reddy,, M. Purser,, W. J. Britt,, and S. B. Boppana. 2009. Enzyme-linked immunosorbent assay method for detection of cytomegalovirus strain-specific antibody responses. Clin. Vaccine Immunol. 16:288290.
86. O’Connor, S.,, C. Taylor,, L. A. Campbell,, S. Epstein,, and P. Libby. 2001. Potential infectious etiologies of atherosclerosis: a multifactorial perspective. Emerg. Infect. Dis. 7:780788.
87. Olive, D. M.,, M. Simsek,, and S. Al-Mufti. 1989. Polymerase chain reaction assay for detection of human cytomegalovirus. J. Clin. Microbiol. 27:12381242.
88. Ono, Y.,, Y. Ito,, K. Kaneko,, Y. Shibata-Watanabe,, T. Tainaka,, W. Sumida,, T. Nak8amura,, H. Kamei,, T. Kiuchi,, H. Ando,, and H. Kimura. 2008. Simultaneous monitoring by real-time polymerase chain reaction of Epstein-Barr virus, human cytomegalovirus, and human herpesvirus-6 in juvenile and adult liver transplant recipients. Transplant. Proc. 40:35783582.
89. Osawa, R.,, and N. Singh. 2009. Cytomegalovirus infection in critically ill patients: a systematic review. Crit. Care 13:R68. doi:10.1186/cc7875.
90. Pang, X. L.,, J. D. Fox,, J. M. Fenton,, G. G. Miller,, A. M. Caliendo,, and J. K. Preiksaitis for the American Society of Transplantation Infectious Diseases Community of Practice and the Canadian Society of Transplantation. 2009. Interlaboratory comparison of cytomegalovirus viral load assays. Am. J. Transplant. 9:258268.
91. Patel, R.,, D. W. Klein,, M. J. Espy,, W. S. Harmsen,, D. M. Ilstrup,, C. V. Paya,, and T. F. Smith. 1995. Optimization of detection of cytomegalovirus viremia in transplantation recipients by shell vial assay. J. Clin. Microbiol. 33:29842986.
92. Paya, C. V.,, A. D. Wold,, D. M. Ilstrup,, and T. F. Smith. 1988. Evaluation of number of shell vial cell cultures per clinical specimen for rapid diagnosis of cytomegalovirus infection. J. Clin. Microbiol. 26:198200.
93. Pellegrin, I.,, I. Garrigue,, D. Ekouevi,, L. Couzi,, P. Merville,, P. Merel,, G. Chene,, M. H. Schrive,, P. Trimoulet,, M. E. Lafon,, and H. Fleury. 2000. New molecular assays to predict occurrence of cytomegalovirus disease in renal transplant recipients. J. Infect. Dis. 182:3642.
94. Percivalle, E.,, E. Genini,, A. Chiesa,, and G. Gerna. 2008. Comparison of a new Light Diagnostics and the CMV Brite to an in-house developed human cytomegalovirus antigenemia assay. J. Clin. Virol. 43:1317.
95. Phipps, P. H.,, L. Gregoire,, E. Rossier,, and E. Perry. 1983. Comparison of five methods of cytomegalovirus antibody screening of blood donors. J. Clin. Microbiol. 18:12961300.
96. Pignatelli, S.,, P. Dal Monte,, G. Rossini,, and M. P. Landini. 2004. Genetic polymorphisms among human cytomegalovirus (HCMV) wild-type strains. Rev. Med. Virol. 14:383410.
97. Prince, H. E.,, and A. L. Leber. 2002. Validation of an inhouse assay for cytomegalovirus immunoglobulin G (CMV IgG) avidity and relationship of avidity to CMV IgM levels. Clin. Diagn. Lab. Immunol. 9:824827.
98. Rafailidis, P. I.,, E. G. Mourtzoukou,, I. C. Varbobitis,, and M. E. Falagas. 2008. Severe cytomegalovirus infection in apparently immunocompetent patients: a systematic review. Virol. J. 5:47. doi:10.1186/1743-422X-5-47.
99. Rasmussen, L.,, S. Morris,, D. Zipeto,, J. Fessel,, R. Wolitz,, A. Dowling,, and T. C. Merigan. 1995. Quantitation of human cytomegalovirus DNA from peripheral blood cells of human immunodeficiency virus-infected patients could predict cytomegalovirus retinitis. J. Infect. Dis. 171:177182.
100. Razonable, R. R.,, R. A. Brown,, M. J. Espy,, A. Rivero,, W. Kremers,, J. Wilson,, C. Groettum,, T. F. Smith,, and C. V. Paya. 2001. Comparative quantitation of cytomegalovirus (CMV) DNA in solid organ transplant recipients with CMV infection by using two high-throughput automated systems. J. Clin. Microbiol. 39:44724476.
101. Razonable, R. R.,, R. A. Brown,, J. Wilson,, C. Groettum,, W. Kremers,, M. Espy,, T. F. Smith,, and C. Paya. 2002. The clinical use of various blood compartments for cytomegalovirus (CMV) DNA quantitation in transplant recipients with CMV disease. Transplantation 73:968973.
102. Razonable, R. R.,, and V. C. Emery. 2004. Management of CMV infection and disease in transplant patients. Herpes 11:7786.
103. Revello, M. G.,, and G. Gerna. 2002. Diagnosis and management of human cytomegalovirus infection in the mother, fetus, and newborn infant. Clin. Microbiol. Rev. 15:680715.
104. Revello, M. G.,, E. Percivalle,, A. Sarasini,, F. Baldanti,, M. Furione,, and G. Gerna. 1994. Diagnosis of human cytomegalovirus infection of the nervous system by pp65 detection in polymorphonuclear leukocytes of cerebrospinal fluid from AIDS patients. J. Infect. Dis. 170:12751279.
105. Salzberger, B.,, D. Myerson,, and M. Boeckh. 1997. Circulating cytomegalovirus (CMV)-infected endothelial cells in marrow transplant patients with CMV disease and CMV infection. J. Infect. Dis. 176:778781.
106. Sanchez, J. L.,, and G. A. Storch. 2002. Multiplex, quantitative, real-time PCR assay for cytomegalovirus and human DNA. J. Clin. Microbiol. 40:23812386.
107. Sanghavi, S. K.,, K. Abu-Elmagd,, M. C. Keightley,, K. St. George,, K. Lewandowski,, S. S. Boes,, A. Bullotta,, R. Dare,, M. Lassak,, S. Jusain,, E. J. Kwak,, D. L. Paterson,, C. R. Rinaldo. 2008. Relationship of cytomegalovirus load assessed by real-time PCR to pp65 antigenemia in organ transplant recipients. J. Clin. Virol. 42:335342.
108. Schaade, L.,, P. Kockelkorn,, K. Ritter,, and M. Kleines. 2000. Detection of cytomegalovirus DNA in human specimens by LightCycler PCR. J. Clin. Microbiol. 38:40064009.
109. Schleiss, M. R.,, and T. C. Heineman. 2005. Progress toward an elusive goal: current status of cytomegalovirus vaccines. Expert Rev. Vaccines 4:381406.
110. Shibata, D.,, W. J. Martin,, M. D. Appelman,, D. M. Causey,, J. M. Leedom,, and N. Arnheim. 1988. Detection of cytomegalovirus DNA in peripheral blood of patients with human immunodeficiency virus. J. Infect. Dis. 158:11851192.
111. Shinkai, M.,, and S. Spector. 1995. Quantitation of human cytomegalovirus (HCMV) DNA in cerebrospinal fluid by competitive PCR in AIDS patients with different HCMV central nervous system diseases. Scand. J. Infect. Dis. 27:559561.
112. Sia, I. G.,, J. A. Wilson,, M. J. Espy,, C. V. Paya,, and T. F. Smith. 2000. Evaluation of the COBAS AMPLICOR CMV MONITOR Test for detection of viral DNA in specimens taken from patients after liver transplantation. J. Clin. Microbiol. 38:600606.
113. Smith, I. L.,, J. M. Cherrington,, R. E. Jiles,, M. D. Fuller,, W. R. Freeman,, and S. A. Spector. 1997. High-level resistance of cytomegalovirus to ganciclovir is associated with alterations in both the UL97 and DNA polymerase genes. J. Infect. Dis. 176:6977.
114. Smith, T. F.,, M. J. Espy,, J. Mandrekar,, M. F. Jones,, F. R. Cockerill,, and R. Patel. 2007. Quantitative realtime polymerase chain reaction for evaluating DNAemia due to cytomegalovirus, Epstein-Barr virus, and BK virus in solid-organ transplant recipients. Clin. Infect. Dis. 45:10561061.
115. Spector, S. A.,, R. Merrill,, D. Wolf,, and W. M. Dankner. 1992. Detection of human cytomegalovirus in plasma of AIDS patients during acute visceral disease by DNA amplification. J. Clin. Microbiol. 30:23592365.
116. Stanier, P.,, D. L. Taylor,, A. D. Kitchen,, N. Wales,, Y. Tryhorn,, and A. S. Tyms. 1989. Persistence of cytomegalovirus in mononuclear cells in peripheral blood from blood donors. Br. Med. J. 299:897898.
117. St. George, K.,, M. J. Boyd,, S. M. Lipson,, D. Ferguson,, G. F. Cartmell,, L. H. Falk,, C. R. Rinaldo,, and M. L. Landry. 2000. A multisite trial comparing two cytomegalovirus (CMV) pp65 antigenemia test kits, Biotest CMV Brite and Bartels/Argene CMV antigenemia. J. Clin. Microbiol. 38:14301433.
118. Tan, B. H.,, N. L. Chlebicka,, J. G. Hong Low,, T. Y. R. Chong,, K. P. Chan,, and Y. T. Goh. 2008. Use of the cytomegalovirus pp65 antigenemia assay for preemptive therapy in allogeneic hematopoietic stem cell transplantation: a realworld review. Transpl. Infect. Dis. 10:325332.
119. Tang, W.,, S. H. Elmore,, H. Fan,, L. B. Thorne,, and M. L. Gulley. 2008. Cytomegalovirus DNA measurement in blood and plasma using Roche LightCycler CMV quantitative reagents. Diagn. Mol. Pathol. 17:166173.
120. The, T. H.,, A. P. van den Berg,, M. C. Harmsen,, W. van der Bij,, and W. J. van Son. 1995. The cytomegalovirus antigenemia assay: a plea for standardization. Scand. J. Infect. Dis. 99 (Suppl.):2529.
121. The, T. H.,, M. van der Ploeg,, A. P. van den Berg,, A. M. Vlieger,, M. van der Giessen,, and W. J. van Son. 1992. Direct detection of cytomegalovirus in peripheral blood leukocytes—a review of the antigenemia assay and polymerase chain reaction. Transplantation 54:193198.
122. Theiler, R. N.,, A. M. Caliendo,, S. Pargman,, B. D. Raynor,, S. Berga,, M. McPheeters,, and D. J. Jamieson. 2006. Umbilical cord blood screening for cytomegalovirus DNA by quantitative PCR. J. Clin. Virol. 37:313316.
123. Tong, C. Y.,, L. E. Cuevas,, H. Williams,, and A. Bakran. 2000. Comparison of two commercial methods for measurement of cytomegalovirus load in blood samples after renal transplantation. J. Clin. Microbiol. 38:12091213.
124. van den Berg, A. P.,, A. M. Tegzess,, A. Scholten-Sampson,, J. Schirm,, M. vanderGiessen,, T. H. The,, and W. J. van Son. 1992. Monitoring antigenemia is useful in guiding treatment of severe cytomegalovirus disease after organ transplantation. Transpl. Int. 5:101107.
125. Verschuuren, E. A.,, M. C. Harmsen,, P. C. Limburg,, W. van der Bij,, A. P. van den Berg,, A. M. Kas-Deelen,, B. Meedendorp,, W. J. van Son,, and T. H. The. 1999. Toward standardization of the human cytomegalovirus antigenemia assay. Intervirology 42:382389.
126. Visser, C. E.,, C. J. van Zeijl,, E. P. de Klerk,, B. M. Schillizi,, M. F. Beersma,, and A. C. Kroes. 2000. First experiences with an accelerated CMV antigenemia test: CMV Brite Turbo assay. J. Clin. Virol. 17:6568.
127. Vornhagen, R.,, B. Plachter,, W. Hinderer,, T. H. The,, J. van Zanten,, L. Matter,, C. A. Schmidt,, H.-H. Sonneborn,, and G. Jahn. 1994. Early serodiagnosis of acute human cytomegalovirus infection by enzyme-linked immunosorbent assay using recombinant antigens. J. Clin. Microbiol. 32:981986.
128. Wada, K.,, N. Kubota,, Y. Ito,, H. Yagasaki,, K. Kato,, T. Yoshikawa,, Y. Ono,, H. Ando,, Y. Fujimoto,, T. Kiuchi,, S. Kojima,, Y. Nishiyama,, and H. Kimura. 2007. Simultaneous quantification of Epstein-Barr virus, cytomegalovirus, and human herpesvirus 6 DNA in samples from transplant recipients by multiplex real-time PCR assay. J. Clin. Microbiol. 45:14261432.
129. Walter, S.,, C. Atkinson,, M. Sharland,, P. Rice,, E. Raglan,, V. C. Emery,, and P. D. Griffiths. 2009. Congenital cytomegalovirus: association between dired blood spot viral load and hearing loss. Arch. Dis. Child Fetal Neonatal Ed. 93:F280F285.
130. Wattanamano, P.,, J. L. Clayton,, J. J. Kopicko,, P. Kissinger,, S. Elliot,, C. Jarrott,, S. Rangan,, and M. A. Beilke. 2000. Comparison of three assays for cytomegalovirus detection in AIDS patients at risk for retinitis. J. Clin. Microbiol. 38:727732.
131. West, P. G.,, B. Aldrich,, R. Hartwig,, and G. J. Haller. 1988. Enhanced detection of cytomegalovirus in confluent MRC-5 cells treated with dexamethasone and dimethyl sulfoxide. J. Clin. Microbiol. 26:25102514.
132. West, P. G.,, and W. W. Baker. 1990. Enhancement by calcium of the detection of cytomegalovirus in cells treated with dexamethasone and dimethyl sulfoxide. J. Clin. Microbiol. 28:17081710.
133. Wolf, D. G.,, and S. A. Spector. 1992. Diagnosis of human cytomegalovirus central nervous system disease in AIDS patients by DNA amplification from cerebrospinal fluid. J. Infect. Dis. 166:14121415.
134. Wolff, D. J.,, D. L. Heaney,, P. D. Neuwald,, K. A. Stellrecht,, and R. D. Press. 2009. Multi-site PCR-based CMV viral load assessment-assays demonstrate linearity and precision, but lack numeric standardization. J. Mol. Diagn. 11:8792.
135. Wunderli, W.,, M. K. Kagi,, E. Gruter,, and J. D. Auracher. 1989. Detection of cytomegalovirus in peripheral leukocytes by different methods. J. Clin. Microbiol. 27:19161917.
136. Zipeto, D.,, F. Baldanti,, D. Zella,, M. Furione,, A. Cavicchini,, G. Milanesi,, and G. Gerna. 1993. Quantification of human cytomegalovirus DNA in peripheral blood polymorphonuclear leukocytes of immunocompromised patients by the polymerase chain reaction. J. Virol. Methods 44:4556.
137. Zipeto, D.,, S. Morris,, C. Hong,, A. Dowling,, R. Wolitz,, T. C. Merigan,, and L. Rasmussen. 1995. Human cytomegalovirus (CMV) DNA in plasma reflects quantity of CMV DNA present in leukocytes. J. Clin. Microbiol. 33:26072611.

Tables

Generic image for table
TABLE 1

Available commercial CMV antigenemia assays

CMV Brite, CMV Brite Turbo, and CINAkit are FDA licensed for qualitative testing only; Light Diagnostics kit is not FDA licensed

Citation: Hodinka R. 2011. Human Cytomegalovirus, p 1558-1574. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch98
Generic image for table
TABLE 2

Available commercial molecular assays for CMV

Abbreviations: pol, polymerase; pp65, phosphoprotein 65; MIE, major immediate early; IE1, immediate early 1; ASR, analyte-specific reagent; RUO, research use only.

Citation: Hodinka R. 2011. Human Cytomegalovirus, p 1558-1574. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch98

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