1887

Chapter 21 : DNA Methylation and Mismatch Repair

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $15.00

Preview this chapter:
Zoom in
Zoomout

DNA Methylation and Mismatch Repair, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555816810/9781555815387_Chap21-1.gif /docserver/preview/fulltext/10.1128/9781555816810/9781555815387_Chap21-2.gif

Abstract:

Sucrose gradient sedimentation of F- is used to demonstrate physical transfer of the element from donor to recipient at the non-permissive temperature. Little was known about DNA methylation at that time (1971) other than that 6-methyladenine (6-meA) and 5-methylcytosine (5-meC) were present in DNA. cells were mutagenized with ethyl methanesulfonate and incorporated in a soft agar layer on minimal agar lacking isoleucine and valine but containing a high level of ampicillin. The best approach to isolate mutants deficient in methylation was decided. The procedure used was based on two prior observations. First, it was known that DNA isolated from grown in the presence of ethionine, a methionine analog, was deficient in methylation because it was a substrate for the transfer of methyl groups from S-adenosyl-methionine (SAM) to DNA in crude extracts. Second, Herb Boyer’s lab had located the gene (near his) for cytosine methylation on the K-12 map by using this assay on recombinants obtained from crosses between K-12 and B, which does not have methylated cytosine in its DNA. The 14 DNA methylation mutants were grown with tritiated methionine and the amount of 6-meA and 5-meC was quantified. This led to the identification of three mutants lacking 6-meA and 11 lacking 5-meC. The isolation of suppressor strains was the author's entry into the DNA mismatch repair field. In the DNA repair world, N-methyl-N'- nitro-N-nitrosoguanidine (MNNG) is associated with base excision repair (BER) and cisplatin with nucleotide excision repair (NER).

Citation: Marinus M. 2011. DNA Methylation and Mismatch Repair, p 211-218. In Maloy S, Hughes K, Casadesús J (ed), The Lure of Bacterial Genetics. ASM Press, Washington, DC. doi: 10.1128/9781555816810.ch21

Key Concept Ranking

Gene Expression and Regulation
0.8339658
Nucleotide Excision Repair
0.48980668
Base Excision Repair
0.4841767
DNA Damage and Repair
0.47289675
Chromosomal DNA
0.457168
0.8339658
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

Figures

Image of FIGURE 1
FIGURE 1

When sharing strains was free and easy. A letter from John Roth accompanying some strains I requested.

Citation: Marinus M. 2011. DNA Methylation and Mismatch Repair, p 211-218. In Maloy S, Hughes K, Casadesús J (ed), The Lure of Bacterial Genetics. ASM Press, Washington, DC. doi: 10.1128/9781555816810.ch21
Permissions and Reprints Request Permissions
Download as Powerpoint

References

/content/book/10.1128/9781555816810.ch21
1. Claverys, J. P., and, S. A. Lacks. 1986. Hetero-duplex deoxyribonucleic acid base mismatch repair in bacteria. Microbiol. Rev. 50:133165.
2. Karran, P., and, M. G. Marinus. 1982. Mismatch correction at O6-methylguanine residues in E. coli DNA. Nature 296:868869.
3. Kuzminov, A. 1995. Collapse and repair of replication forks in Escherichia coli. Mol. Microbiol. 16:373384.
4. Loutit, J. S., and, M. G. Marinus. 1968. Investigation of the mating system of Pseudomonas aeruginosa strain 1. II. Mapping of a number of early markers. Genet. Res. 12:3744.
5. Marinus, M. G., and, E. A. Adelberg. 1970. Vegetative replication and transfer replication of deoxyribonucleic acid in temperature-sensitive mutants of Escherichia coli K-12. J. Bacteriol. 104:12661272.
6. Marinus, M. G., and, E. B. Konrad. 1976. Hyper-recombination in dam mutants of Escherichia coli K-12. Mol. Gen. Genet. 149:273277.
7. Marinus, M. G., and, N. R. Morris. 1973. Isolation of deoxyribonucleic acid methylase mutants of Escherichia coli K-12.J. Bacteriol. 114:11431150.
8. Marinus, M. G., and, N. R. Morris. 1974. Biological function for 6-methyladenine residues in the DNA of Escherichia coli K12. J. Mol. Biol. 85: 309322.
9. McGraw, B. R., and, M. G. Marinus. 1980. Isolation and characterization of Dam+ revertants and suppressor mutations that modify secondary phenotypes of dam-3 strains of Escherichia coli K-12. Mol. Gen. Genet. 178:309315.
10. Meselson, M. 1988. Methyl-directed repair of DNA mismatches, p. 91113. In K. B. Low (ed.), The Recombination of Genetic Material. Academic Press, San Diego, CA.
11. Nowosielska, A., and, M. G. Marinus. 2008. DNA mismatch repair-induced double-strand breaks. DNA Repair 7:4856.
12. Pukkila, P. J.,, J. Peterson,, G. Herman,, P. Modrich, and, M. Meselson. 1983. Effects of high levels of DNA adenine methylation on methyl-directed mismatch repair in Escherichia coli. Genetics 104:571582.

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error