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Chapter 23 : Finding Inversions

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Finding Inversions, Page 1 of 2

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Abstract:

The author's project at University of Utah was an interesting mix of bacterial genetics and evolutionary biology, which aimed to gain understanding of what evolutionary forces would preserve the order of genes in the and serovar Typhimurium genomes over the 200 million years of separation between the two species. The basic idea was to change the genome and see what happened, and the mechanism to make the chromosome rearrangements was to use Tn as a portable region of homology. A selection/screen was designed to find inversions, based on the -203 deletion selection that was first used by Ames, Hartman, and Jacob in 1963. The flurry of subsequent tests—nontransducibility of both the operon and the auxotrophy—had been predicted on paper, and all quickly bore out to be pretty much true. With the confidence of knowing that inversions would arise from the -203 screen, the author went searching for nonauxotrophic inversions by using the non-transducibility of the inversion breakpoint to identify them. This was a massive cross-streaking exercise, but much less work than the initial auxotrophy screen. The only problem was that the tools to characterize the inversions, to figure out the location of the second end point, were going to be difficult and ultimately not very satisfying. What emerged from the initial mess of crosses was a method to map the end points of the nonauxotrophic inversions, which was really unexpected and amazing.

Citation: Schmid M. 2011. Finding Inversions, p 227-231. In Maloy S, Hughes K, Casadesús J (ed), The Lure of Bacterial Genetics. ASM Press, Washington, DC. doi: 10.1128/9781555816810.ch23

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Bacterial Genetics
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Salmonella enterica
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Escherichia coli
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Figures

Image of FIGURE 1
FIGURE 1

Taking advantage of the operon to select for inversions.

Citation: Schmid M. 2011. Finding Inversions, p 227-231. In Maloy S, Hughes K, Casadesús J (ed), The Lure of Bacterial Genetics. ASM Press, Washington, DC. doi: 10.1128/9781555816810.ch23
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Image of FIGURE 2
FIGURE 2

Genetic mapping to determine the location ofjoin points in the prototrophic inversions.

Citation: Schmid M. 2011. Finding Inversions, p 227-231. In Maloy S, Hughes K, Casadesús J (ed), The Lure of Bacterial Genetics. ASM Press, Washington, DC. doi: 10.1128/9781555816810.ch23
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References

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