Chapter 27 : Baltimore and Beyond: from to the Postgenomic Era

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Serendipity led the author from Baltimore, New Haven, and Palo Alto to Wilmington, DE. Mentors along the journey taught the power of combining seemingly disparate techniques while striving for biological understanding. Those lessons, and the continuous growth they have engendered, have been a beacon for our microbial genetics effort that has blurred the divide between fundamental and applied inquiry. Interested in American history and genetics, the author went to Baltimore in 1969 to attend Johns Hopkins. Talking about Phil Hartman, the author says his text, , is treasured both for its concise presentation of the mechanisms of gene expression and for its prescience to consider secondary consequences of gene action. The author adds that what was important was the scientific result rather than who accomplished it. So believing that we knew a great deal about gene action including pleiotropy, the author was looking for a new horizon and the molecular basis of microbial development seemed attractive. The author says Hartman's chapter, "Secondary Consequences of Gene Mutation," in was the guidepost to follow. Inhibition was observed by the desperate measure of placing amino acid crystals on plates, a technique that the author first used in Hartman's genetics lab course in Baltimore. Raising the media supplementation concentrations to approximately 0.5 mg/ml often allows inhibition to be observed.

Citation: LaRossa R. 2011. Baltimore and Beyond: from to the Postgenomic Era, p 267-275. In Maloy S, Hughes K, Casadesús J (ed), The Lure of Bacterial Genetics. ASM Press, Washington, DC. doi: 10.1128/9781555816810.ch27

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Aromatic Amino Acids
Microbial Genetics
Amino Acids
Wastewater Treatment Plants
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