Chapter 11 : Heterologous Protein Expression in Yeasts and Filamentous Fungi

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Many filamentous fungi and yeast species have been used for heterologous protein. This chapter covers the most commonly used fungal expression systems (, , and species), with a focus on vector systems, promoter and leader sequences, posttranslational modifications, and fermentation scalability. Similar to other heterologous protein expression systems, proteolytic hydrolysis often causes instability of foreign proteins in . In comparison with and , filamentous fungi are less studied for heterologous protein production with respect to their transcription and translation control, mRNA stability, glycosylation, and regulation of protein secretion. There are several web-accessible organizations such as the American Type Culture Collection (ATCC), the (Fungal Genetics Stock Center (FGSC), the Agricultural Research Service Culture Collection (NRRL), and the Belgium Culture Collection (BCCP) from which the necessary tools for heterologous protein expression in filamentous fungi can be obtained. High mannose-type glycosylation is observed in filamentous fungi, but it is much less extensive than that seen in proteins produced in and species. Greater understanding of transcriptional control, mRNA stability, glycosylation, protein folding, and other posttranslational processes will allow us to overcome the difficulties associated with heterologous protein expression in fungi, such as hyperglycosylation in yeasts and poor secretion associated with some mammalian proteins. The exponential increase in fungal genomics and proteomics in recent years will no doubt fuel the advancement of filamentous fungi as heterologous protein expression hosts.

Citation: Zhang N, An Z. 2010. Heterologous Protein Expression in Yeasts and Filamentous Fungi, p 145-156. In Baltz R, Demain A, Davies J, Bull A, Junker B, Katz L, Lynd L, Masurekar P, Reeves C, Zhao H (ed), Manual of Industrial Microbiology and Biotechnology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816827.ch11

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Pros and cons for some commonly used heterologous protein expression systems

Citation: Zhang N, An Z. 2010. Heterologous Protein Expression in Yeasts and Filamentous Fungi, p 145-156. In Baltz R, Demain A, Davies J, Bull A, Junker B, Katz L, Lynd L, Masurekar P, Reeves C, Zhao H (ed), Manual of Industrial Microbiology and Biotechnology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816827.ch11
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Vector systems used for heterologous protein expression in

Citation: Zhang N, An Z. 2010. Heterologous Protein Expression in Yeasts and Filamentous Fungi, p 145-156. In Baltz R, Demain A, Davies J, Bull A, Junker B, Katz L, Lynd L, Masurekar P, Reeves C, Zhao H (ed), Manual of Industrial Microbiology and Biotechnology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816827.ch11
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Selectable markers used for heterologous gene expression in species

Citation: Zhang N, An Z. 2010. Heterologous Protein Expression in Yeasts and Filamentous Fungi, p 145-156. In Baltz R, Demain A, Davies J, Bull A, Junker B, Katz L, Lynd L, Masurekar P, Reeves C, Zhao H (ed), Manual of Industrial Microbiology and Biotechnology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816827.ch11
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Promoters used in heterologous gene expression in species

Citation: Zhang N, An Z. 2010. Heterologous Protein Expression in Yeasts and Filamentous Fungi, p 145-156. In Baltz R, Demain A, Davies J, Bull A, Junker B, Katz L, Lynd L, Masurekar P, Reeves C, Zhao H (ed), Manual of Industrial Microbiology and Biotechnology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816827.ch11

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