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Chapter 39 : Accessing Microbial Communities Relevant to Biofuels Production

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Abstract:

Using 16S rRNA and 18S rRNA as the most commonly used molecules to classify and , the ability to digest cellulose is widely distributed. With very few exceptions, anaerobes degrade cellulose primarily via complexed cellulose systems, while aerobic cellulose degraders utilize cellulose through the production of substantial amounts of extracellular cellulases secreted into the culture supernatant, leading to two primary strategies for crystalline cellulose utilization. To further explore the diversity of novel cellulose-degrading microorganisms, novel cultivation methods need to be explored. Cell separation technologies use optical instrumentation to visualize the specific microorganisms in conjunction with cell manipulation methods. Cell manipulation is dominated by two principal methods: laser manipulation and mechanical manipulation. The traditional cultivation methods, based on plating of mixed populations of microorganisms on solidified medium such as agar, are selective and biased toward growth of specific microorganisms. Methods to extract DNA from soils and sediments are generally classified as direct or indirect extractions. Any standard DNA hybridization probe approach will cover only a very small percentage of the potential within a typical metagenomic library. Numerous studies offering comparisons of metagenomes and functional insights into microbial communities have been published and will continue to be invaluable.

Citation: Abulencia C, Wells S, Gray K, Keller M, Kreps J. 2010. Accessing Microbial Communities Relevant to Biofuels Production, p 565-576. In Baltz R, Demain A, Davies J, Bull A, Junker B, Katz L, Lynd L, Masurekar P, Reeves C, Zhao H (ed), Manual of Industrial Microbiology and Biotechnology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816827.ch39

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Bacterial Cell Wall
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Microbial Ecology
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Reverse Transcriptase PCR
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Next-Generation Sequencing Methods
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Agarose Gel Electrophoresis
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References

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1. Altschul, S. F.,, W. Gish,, W. Miller,, E. W. Myers, and, D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403410.
2. Amann, R. I.,, W. Ludwig, and, K. H. Schleifer, 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:143169.
3. Ashkin, A.,, J. M. Dziedzic, and, T. Yamane. 1987. Optical trapping and manipulation of single cells using infrared laser beams. Nature 330:769771.
4. Berry, A. E.,, C. Chiocchini,, T. Selby,, M. Sosio, and, E. M. Wellington. 2003. Isolation of high molecular weight DNA from soil for cloning into BAC vectors. FEMS Microbiol. Lett. 223:1520.
5. Blumer-Schuette, S. E.,, I. Kataeva,, J. Westpheling,, M. W. Adams, and, R. M. Kelly. 2008. Extremely thermo-philic microorganisms for biomass conversion: status and prospects. Curr. Opin. Biotechnol. 19:210217.
6. Brennan, Y.,, W. N. Callen,, L. Christoffersen,, P. Dupree,, F. Goubet,, S. Healey,, M. Hernandez,, M. Keller,, K. Li,, N. Palackal,, A. Sittenfeld,, G. Tamayo,, S. Wells,, G. P. Hazlewood,, E. J. Mathur,, J. M. Short,, D. E. Robertson, and, B. A. Steer. 2004. Unusual microbial xylanases from insect guts. Appl. Environ. Microbiol. 70:36093617.
7. Brulc, J. M.,, D. A. Antonopoulos,, M. E. Miller,, M. K. Wilson,, A. C. Yannarell,, E. A. Dinsdale,, R. E. Edwards,, E. D. Frank,, J. B. Emerson,, P. Wacklin,, P. M. Coutinho,, B. Henrissat,, K. E. Nelson, and, B. A. White. 2009. Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydro-lases. Proc. Natl. Acad. Sci. USA 106:19481953.
8. Bruns, A.,, H. Hoffelner, and, J. Overmann. 2003. A novel approach for high throughput cultivation assays and the isolation of planktonic bacteria. FEMS Microbiol. Ecol. 45:161171.
9. Bruns, A.,, U. Nubel,, H. Cypionka, and, J. Overmann. 2003. Effect of signal compounds and incubation conditions on the culturability of freshwater bacterioplankton. Appl. Environ. Microbiol. 69:19801989.
10. Button, D. K.,, F. Schut,, P. Quang,, R. Martin, and, B. R. Robertson. 1993. Viability and isolation of marine bacteria by dilution culture: theory, procedures, and initial results. Appl. Environ. Microbiol. 59:881891.
11. Chin, K. J.,, D. Hahn,, U. Hengstmann,, W. Liesack, and, P. H. Janssen. 1999. Characterization and identification of numerically abundant culturable bacteria from the anoxic bulk soil of rice paddy microcosms. Appl. Environ. Microbiol. 65:50425049.
12. Connon, S. A., and, S. J. Giovannoni. 2002. High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates. Appl. Environ. Microbiol. 68:38783885.
13. Cook, D. M.,, E. DeCrescenzo Henriksen,, R. Upchurch, and, J. B. Doran Peterson. 2007. Isolation of polymer-degrading bacteria and characterization of the hindgut bacterial community from the detritus-feeding larvae of Tipula abdominalis (Diptera: Tipulidae). Appl. Environ. Microbiol. 73:56835686.
14. Davey, H. M., and, D. B. Kell. 1996. Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses. Microbiol. Rev. 60:641696.
15. Eilers, H.,, J. Pernthaler,, F. O. Glockner, and, R. Amann. 2000. Culturability and in situ abundance of pelagic bacteria from the North Sea. Appl. Environ. Microbiol. 66:30443051.
16. Ferrari, B. C.,, T. Winsley,, M. Gillings, and, S. Binnerup. 2008. Cultivating previously uncultured soil bacteria using a soil substrate membrane system. Nat. Protoc. 3:12611269.
17. Finn, R. D.,, J. Tate,, J. Mistry,, P. C. Coggill,, S. J. Sammut,, H. R. Hotz,, G. Ceric,, K. Forslund,, S. R. Eddy,, E. L. Sonnhammer, and, A. Bateman. 2008. The Pfam protein families database. Nucleic Acids Res. 36:D281D288.
18. Freier, D.,, C. P. Mothershed, and, J. Wiegel. 1988. Characterization of Clostridium thermocellum JW20. Appl. Environ. Microbiol. 54:204211.
19. Fröhlich, J., and H. König. 1999. Rapid isolation of single microbial cells from mixed natural and laboratory populations with the aid of a micromanipulator. Syst. Appl. Microbiol. 22:249257.
20. Fröhlich, J., and H. König. 2000. New techniques for isolation of single prokaryotic cells. FEMS Microbiol. Rev. 24:567572.
21. Fu, A. Y.,, H. P. Chou,, C. Spence,, F. H. Arnold, and, S. R. Quake. 2002. An integrated microfabricated cell sorter. Anal. Chem. 74:24512457.
22. Fu, A. Y.,, C. Spence,, A. Scherer,, F. H. Arnold, and, S. R. Quake. 1999. A microfabricated fluorescence-activated cell sorter. Nat. Biotechnol. 17:11091111.
23. Gavrish, E.,, A. Bollmann,, S. Epstein, and, K. Lewis. 2008. A trap for in situ cultivation of filamentous actino-bacteria. J. Microbiol. Methods 72:257262.
24. Gray, K. A.,, T. H. Richardson,, D. E. Robertson,, P. E. Swanson, and, M. V. Subramanian. 2003. Soil-based gene discovery: a new technology to accelerate and broaden biocatalytic applications. Adv. Appl. Microbiol. 52:127.
25. Guan, L. L.,, H. Onuki, and, K. Kamino. 2000. Bacterial growth stimulation with exogenous siderophore and synthetic N-acyl homoserine lactone autoinducers under iron-limited and low-nutrient conditions. Appl. Environ. Microbiol. 66:27972803.
26. Handelsman, J.,, M. R. Rondon,, S. F. Brady,, J. Clardy, and, R. M. Goodman. 1998. Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem. Biol. 5:R245R249.
27. Huber, R.,, S. Burggraf,, T. Mayer,, S. M. Barns,, P. Rossnagel, and, K. O. Stetter. 1995. Isolation of a hyper-thermophilic archaeum predicted by in situ RNA analysis. Nature 376:5758.
28. Hugenholtz, P., and, G. W. Tyson. 2008. Microbiology: metagenomics. Nature 455:481483.
29. Hugenholtz, P.,, G. W. Tyson,, R. I. Webb,, A. M. Wagner, and, L. L. Blackall. 2001. Investigation of candidate division TM7, a recently recognized major lineage of the domain Bacteria with no known pure-culture representatives. Appl. Environ. Microbiol. 67:411419.
30. Irwin, D. M., and, A. C. Wilson. 1990. Concerted evolution of ruminant stomach lysozymes. Characterization of lysozyme cDNA clones from sheep and deer. J. Biol. Chem. 265:49444952.
31. Ishoy, T.,, T. Kvist,, P. Westermann, and, B. K. Ahring. 2006. An improved method for single cell isolation of prokaryotes from meso-, thermo- and hyperthermophilic environments using micromanipulation. Appl. Microbiol. Biotechnol. 69:510514.
32. Jacobsen, C. S., and, O. F. Rasmussen. 1992. Development and application of a new method to extract bacterial DNA from soil based on separation of bacteria from soil with cation-exchange resin. Appl. Environ. Microbiol. 58:24582462.
33. Janssen, P. H.,, P. S. Yates,, B. E. Grinton,, P. M. Taylor, and, M. Sait. 2002. Improved culturability of soil bacteria and isolation in pure culture of novel members of the divisions Acidobacteria, Actinobacteria, Proteobacteria, and Verrucomicrobia. Appl. Environ. Microbiol. 68:23912396.
34. Kaeberlein, T.,, K. Lewis, and, S. S. Epstein. 2002. Isolating “uncultivable” microorganisms in pure culture in a simulated natural environment. Science 296:11271129.
35. Kalyuzhnaya, M. G.,, R. Zabinsky,, S. Bowerman,, D. R. Baker,, M. E. Lidstrom, and, L. Chistoserdova. 2006. Fluorescence in situ hybridization-flow cytometry-cell sorting-based method for separation and enrichment of type I and type II methanotroph populations. Appl. Environ. Microbiol. 72:42934301.
36. Keller, M., and, K. Zengler. 2004. Tapping into microbial diversity. Nat. Rev. Microbiol. 2:141150.
37. Keller, M.,, K. Zengler,, G. Toledo,, J. Elkins,, E. Mathur, and, J. Short. 2002. Microbiology: what is next? Geochim. Cosmochim. Acta 66:A390.
38. Kim, S. J.,, C. M. Lee,, M. Y. Kim,, Y. S. Yeo,, S. H. Yoon,, H. C. Kang, and, B. S. Koo. 2007. Screening and characterization of an enzyme with beta-glucosidase activity from environmental DNA. J. Microbiol. Biotechnol. 17:905912.
39. Krause, L.,, N. N. Diaz,, A. Goesmann,, S. Kelley,, T. W. Nattkemper,, F. Rohwer,, R. A. Edwards, and, J. Stoye. 2008. Phylogenetic classification of short environmental DNA fragments. Nucleic Acids Res. 36:22302239.
40. Kunin, V.,, A. Copeland,, A. Lapidus,, K. Mavromatis, and, P. Hugenholtz. 2008. A bioinformatician’s guide to metagenomics. Microbiol. Mol. Biol. Rev. 72:557578.
41. Lafferty, M. March 2006. GigaMatrix holding tray having through-hole wells. U.S. patent 7,019,827.
42. Lafferty, M., and, M. J. Dycaico. 2004. GigaMatrixTM: an ultra high-throughput tool for accessing biodiversity. J. Assoc. Lab. Automation 9:200208.
43. Lafferty, M., and, M. J. Dycaico. 2004. GigaMatrix: a novel ultrahigh throughput protein optimization and discovery platform. Methods Enzymol. 388:119134.
44. Luo, C.,, H. Li,, C. Xiong,, X. Peng,, Q. Kou,, Y. Chen,, H. Ji, and, Q. Ouyang. 2007. The combination of optical tweezers and microwell array for cells physical manipulation and localization in microfluidic device. Biomed. Mi-crodevices 9:573578.
45. Lynch, J. M. 1988. The terrestrial environment, p. 103131. In J. M. Lynch and, J. E. Hobbie (ed.), Microorganisms in Action: Concepts and Applications in Microbial Ecology. Blackwell Scientific Publishers, Oxford, United Kingdom.
46. Lynd, L. R.,, P. J. Weimer,, W. H. van Zyl, and, I. S. Pretorius. 2002. Microbial cellulose utilization: fundamentals and biotechnology. Microbiol. Mol. Biol. Rev. 66:506577.
47. Marcy, Y.,, C. Ouverney,, E. M. Bik,, T. Losekann,, N. Ivanova,, H. G. Martin,, E. Szeto,, D. Platt,, P. Hugen-holtz,, D. A. Relman, and, S. R. Quake. 2007. Dissecting biological “dark matter” with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth. Proc. Natl. Acad. Sci. USA 104:1188911894.
48. Morales, S. E.,, T. F. Cosart,, J. V. Johnson, and, W. E. Holben. 2009. Extensive phylogenetic analysis of a soil bacterial community illustrates extreme taxon evenness and the effects of amplicon length, degree of coverage, and DNA fractionation on classification and ecological parameters. Appl. Environ. Microbiol. 75:668675.
49. Ogram, A.,, G. Sayler, and, T. Barkay. 1987. The extraction and purification of microbial DNA from sediments. J. Microbiol. Methods 7:5766.
50. Palackal, N.,, C. S. Lyon,, S. Zaidi,, P. Luginbuhl,, P. Dupree,, F. Goubet,, J. L. Macomber,, J. M. Short,, G. P. Hazlewood,, D. E. Robertson, and, B. A. Steer. 2007. A multifunctional hybrid glycosyl hydrolase discovered in an uncultured microbial consortium from ruminant gut. Appl. Microbiol. Biotechnol. 74:113124.
51. Pettersson, E.,, J. Lundeberg, and, A. Ahmadian. 2009. Generations of sequencing technologies. Genomics 93:105111.
52. Podar, M.,, C. B. Abulencia,, M. Walcher,, D. Hutchison,, K. Zengler,, J. A. Garcia,, T. Holland,, D. Cotton,, L. Hauser, and, M. Keller. 2007. Targeted access to the genomes of low-abundance organisms in complex microbial communities. Appl. Environ. Microbiol. 73:32053214.
53. Prieur, D. J. 1986. Tissue specific deficiency of lysozyme in ruminants. Comp. Biochem. Physiol. B 85:349353.
54. Raghunathan, A.,, H. R. Ferguson, Jr.,, C. J. Bornarth,, W. Song,, M. Driscoll, and, R. S. Lasken. 2005. Genomic DNA amplification from a single bacterium. Appl. Environ. Microbiol. 71:33423347.
55. Rajendhran, J., and, P. Gunasekaran. 2008. Strategies for accessing soil metagenome for desired applications. Biotechnol. Adv. 26:576590.
56. Rappe, M. S., and, S. J. Giovannoni. 2003. The uncultured microbial majority. Annu. Rev. Microbiol. 57:369394.
57. Richardson, T. H.,, X. Tan,, G. Frey,, W. Callen,, M. Cabell,, D. Lam,, J. Macomber,, J. M. Short,, D. E. Robertson, and, C. Miller. 2002. A novel, high performance enzyme for starch liquefaction. Discovery and optimization of a low pH, thermostable alpha-amylase. J. Biol. Chem. 277:2650126507.
58. Robertson, D. E.,, J. A. Chaplin,, G. DeSantis,, M. Podar,, M. Madden,, E. Chi,, T. Richardson,, A. Milan,, M. Miller,, D. P. Weiner,, K. Wong,, J. McQuaid,, B. Farwell,, L. A. Preston,, X. Tan,, M. A. Snead,, M. Keller,, E. Mathur,, P. L. Kretz,, M. J. Burk, and, J. M. Short. 2004. Exploring nitrilase sequence space for enantioselective catalysis. Appl. Environ. Microbiol. 70:24292436.
59. Rodriguez-Brito, B.,, F. Rohwer, and, R. A. Edwards. 2006. An application of statistics to comparative metage-nomics. BMC Bioinformatics 7:162.
60. Rusch, D. B.,, A. L. Halpern,, G. Sutton,, K. B. Heidelberg,, S. Williamson,, S. Yooseph,, D. Wu,, J. A. Eisen,, J. M. Hoffman,, K. Remington,, K. Beeson,, B. Tran,, H. Smith,, H. Baden-Tillson,, C. Stewart,, J. Thorpe,, J. Freeman,, C. Andrews-Pfannkoch,, J. E. Venter,, K. Li,, S. Kravitz,, J. F. Heidelberg,, T. Utterback,, Y. H. Rogers,, L. I. Falcon,, V. Souza,, G. Bonilla-Rosso,, L. E. Eguiarte,, D. M. Karl,, S. Sathyendranath,, T. Platt,, E. Bermingham,, V. Gallardo,, G. Tamayo-Castillo,, M. R. Ferrari,, R. L. Strausberg,, K. Neal-son,, R. Friedman,, M. Frazier, and, J. C. Venter. 2007. The Sorcerer II Global Ocean Sampling expedition: northwest Atlantic through eastern tropical Pacific. PLoS Biol. 5:e77.
61. Sait, M.,, P. Hugenholtz, and, P. H. Janssen. 2002. Cultivation of globally distributed soil bacteria from phy-logenetic lineages previously only detected in cultivation-independent surveys. Environ. Microbiol. 4:654666.
62. Schutze, K.,, H. Posl, and, G. Lahr. 1998. Laser micromanipulation systems as universal tools in cellular and molecular biology and in medicine. Cell. Mol. Biol. (Noisy-le-grand) 44:735746.
63. Sekar, R.,, B. M. Fuchs,, R. Amann, and, J. Pernthaler. 2004. Flow sorting of marine bacterioplankton after fluorescence in situ hybridization. Appl. Environ. Microbiol. 70:62106219.
64. Shiratori, H.,, H. Ikeno,, S. Ayame,, N. Kataoka,, A. Miya,, K. Hosono,, T. Beppu, and, K. Ueda. 2006. Isolation and characterization of a new Clostridium sp. that performs effective cellulosic waste digestion in a thermophilic methano-genic bioreactor. Appl. Environ. Microbiol. 72:37023709.
65. Short, J. M. January 2001. Screening methods for enzymes and enzyme kits. U.S. patent 6,168,919.
66. Short, J. M. January 2004. Protein activity screening of clones having DNA from uncultivated microorganisms. U.S. patent 6,677,115.
67. Short, J. M. September 1999. Protein activity screening of clones having DNA from uncultivated microorganisms. U.S. patent 5,958,672.
68. Steele, H. L.,, K. E. Jaeger,, R. Daniel, and, W. R. Streit. 2009. Advances in recovery of novel biocatalysts from metagenomes. J. Mol. Microbiol. Biotechnol. 16:2537.
69. Stein, J. L.,, T. L. Marsh,, K. Y. Wu,, H. Shizuya, and, E. F. DeLong. 1996. Characterization of uncultivated prokary-otes: isolation and analysis of a 40-kilobase-pair genome fragment from a planktonic marine archaeon. J. Bacteriol. 178:591599.
70. Thomsen, T. R.,, J. L. Nielsen,, N. B. Ramsing, and, P. H. Nielsen. 2004. Micromanipulation and further identification of FISH-labelled microcolonies of a dominant denitrifying bacterium in activated sludge. Environ. Microbiol. 6:470479.
71. Tokuda, G., and, H. Watanabe. 2007. Hidden cellulases in termites: revision of an old hypothesis. Biol. Lett. 3:336339.
72. Torsvik, V., and, L. Ovreas. 2002. Microbial diversity and function in soil: from genes to ecosystems. Curr. Opin. Microbiol. 5:240245.
73. Torsvik, V.,, L. Ovreas, and, T. F. Thingstad. 2002. Pro-karyotic diversity—magnitude, dynamics, and controlling factors. Science 296:10641066.
74. Tsai, Y. L., and, B. H. Olson. 1991. Rapid method for direct extraction of DNA from soil and sediments. Appl. Environ. Microbiol. 57:10701074.
75. Tyson, G. W.,, J. Chapman,, P. Hugenholtz,, E. E. Allen,, R. J. Ram,, P. M. Richardson,, V. V. Solovyev,, E. M. Rubin,, D. S. Rokhsar, and, J. F. Banfield. 2004. Community structure and metabolism through reconstruction of microbial genomes from the environment. Nature 428:3743.
76. Venter, J. C.,, K. Remington,, J. F. Heidelberg,, A. L. Halpern,, D. Rusch,, J. A. Eisen,, D. Wu,, I. Paulsen,, K. E. Nelson,, W. Nelson,, D. E. Fouts,, S. Levy,, A. H. Knap,, M. W. Lomas,, K. Nealson,, O. White,, J. Peterson,, J. Hoffman,, R. Parsons,, H. Baden-Tillson,, C. Pfannkoch,, Y. H. Rogers, and, H. O. Smith. 2004. Environmental genome shotgun sequencing of the Sargasso Sea. Science 304:6674.
77. Vives-Rego, J.,, P. Lebaron, and, G. Nebe-von Caron. 2000. Current and future applications of flow cytometry in aquatic microbiology. FEMS Microbiol. Rev. 24:429448.
78. Warnecke, F.,, P. Luginbuhl,, N. Ivanova,, M. Ghas-semian,, T. H. Richardson,, J. T. Stege,, M. Cayouette,, A. C. McHardy,, G. Djordjevic,, N. Aboushadi,, R. Sorek,, S. G. Tringe,, M. Podar,, H. G. Martin,, V. Kunin,, D. Dalevi,, J. Madejska,, E. Kirton,, D. Platt,, E. Szeto,, A. Salamov,, K. Barry,, N. Mikhailova,, N. C. Kyrpides,, E. G. Matson,, E. A. Ottesen,, X. Zhang,, M. Hernandez,, C. Murillo,, L. G. Acosta,, I. Rigoutsos,, G. Tamayo,, B. D. Green,, C. Chang,, E. M. Rubin,, E. J. Mathur,, D. E. Robertson,, P. Hugenholtz, and, J. R. Leadbetter. 2007. Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite. Nature 450:560565.
79. Xie, C.,, J. Mace,, M. A. Dinno,, Y. Q. Li,, W. Tang,, R. J. Newton, and, P. J. Gemperline. 2005. Identification of single bacterial cells in aqueous solution using confocal laser tweezers Raman spectroscopy. Anal. Chem. 77:43904397.
80. Zengler, K.,, G. Toledo,, M. Rappe,, J. Elkins,, E. J. Mathur,, J. M. Short, and, M. Keller. 2002. Cultivating the uncultured. Proc. Natl. Acad. Sci. USA 99:1568115686.
81. Zhou, J.,, M. A. Bruns, and, J. M. Tiedje. 1996. DNA recovery from soils of diverse composition. Appl. Environ. Microbiol. 62:316322.

Tables

Generic image for table
TABLE 1

Metagenome screening

Citation: Abulencia C, Wells S, Gray K, Keller M, Kreps J. 2010. Accessing Microbial Communities Relevant to Biofuels Production, p 565-576. In Baltz R, Demain A, Davies J, Bull A, Junker B, Katz L, Lynd L, Masurekar P, Reeves C, Zhao H (ed), Manual of Industrial Microbiology and Biotechnology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816827.ch39
Generic image for table
TABLE 2

Bacterial cloning systems

Citation: Abulencia C, Wells S, Gray K, Keller M, Kreps J. 2010. Accessing Microbial Communities Relevant to Biofuels Production, p 565-576. In Baltz R, Demain A, Davies J, Bull A, Junker B, Katz L, Lynd L, Masurekar P, Reeves C, Zhao H (ed), Manual of Industrial Microbiology and Biotechnology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816827.ch39

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