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Molecular Microbiology: Diagnostic Principles and Practice, Second Edition

Editors: David H. Persing1, Fred C. Tenover2, Yi-Wei Tang3, Frederick S. Nolte4, Randall T. Hayden5, Alex van Belkum6
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Affiliations: 1: Cepheid Corporation, Sunnyvale, California; 2: Cepheid Corporation, Sunnyvale, California; 3: Vanderbilt University School of Medicine, Nashville, Tennessee; 4: Medical University of South Carolina, Charleston, South Carolina; 5: St. Jude Children’s Research Hospital, Memphis, Tennessee; 6: Erasmus University, Rotterdam, The Netherlands;
Content Type: Reference
Publication Year: 2011

Category: Clinical Microbiology; Bacterial Pathogenesis

The second edition of presents the latest molecular diagnostic techniques to support clinical care and basic and clinical research. The authors-all experienced researchers and diagnosticians-have conducted a comprehensive review and evaluation of this rapidly evolving field and provide the new material in an easy-to-read summary format. Moreover, the book offers a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors.

not only examines techniques to detect individual pathogens, but also explores the growing trend toward a systems approach for diagnosing infectious diseases, with chapters covering gastrointestinal infections, sepsis, meningitis, and encephalitis. In short, this text not only encapsulates the current state of the science, but also points to new avenues for research that will broaden the application and usefulness of molecular diagnostics.

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The Quarterly Review of Biology

04 June 2013

Nucleic acid amplification and related molecular techniques play an increasingly important role in diagnostic microbiology laboratories. Molecular methods are widely used in viral load assays and to detect selected bacteria; many of these tests are now performed with commercial kits.These trends are reflected in the second edition of Molecular Microbiology. The first six sections provide a good overview of molecular techniques, including sample preparation, amplification and detection, strain typing, and new technologies. The remainder of the book focuses on applications in clinical microbiology.

The chapters on HIV, Hepatitis B, and Hepatitis C provide solid reviews on the role of viral load assays in patient management and discuss the current generation of commercial assays as well as methods for detecting mutations that confer resistance to antiviral agents. The chapter on respiratory viruses provides an excellent review of this complex group of organisms and the challenge of developing multiplex assays that simultaneously detect many pathogens, a topic that became particularly important after the emergence of a pandemic strain of H1N1 influenza virus in 2009. Other noteworthy chapters include a discussion of the role of DNA sequencing in bacterial identification and an excellent review on central nervous system infections. The tests discussed in the sections on mycology, parasitology, and host/pathogen interactions are mostly at the investigational stage, although fungal identification by sequencing and amplification tests for malaria are now available in many reference laboratories.

In recent years there has been a marked increase in regulatory oversight of laboratory-developed tests. The terms “verification” and “validation,” which were not listed in the index of the first edition, now have their own chapters and detail the steps required to insure that molecular tests produce accurate results. This volume will be useful for residents, fellows, and laboratory directors who are performing molecular assays or need to help others interpret the results.

The Quarterly Review of Biology

Reviewer: Eric D. Spitzer, Pathology, Stony Brook University, Stony Brook, New York

Review Date: December 2012

Doody's Review Service

02 June 2013

At A Glance

The second edition of Molecular Microbiology: Diagnostic Principles and Practice presents the latest molecular diagnostic techniques to support clinical care and basic and clinical research. The authors all experienced researchers and diagnosticians have conducted a comprehensive review and evaluation of this rapidly evolving field and provide the new material in an easy-to-read summary format. Moreover, the book offers a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors.

Molecular Microbiology not only examines techniques to detect individual pathogens, but also explores the growing trend toward a systems approach for diagnosing infectious diseases, with chapters covering gastrointestinal infections, sepsis, meningitis, and encephalitis. In short, this text not only encapsulates the current state of the science, but also points to new avenues for research that will broaden the application and usefulness of molecular diagnostics.

New to the Second Edition

This edition offers the most current findings, practices, and technology. There are new chapters on microbial phylogeny, nucleic acid sequence analysis, and pharmacogenomics. The book also features several newly updated sections, including sections dedicated to verification and validation of molecular tests, laboratory operations, molecular typing, and new technologies for nucleic acid detection. In addition, the reader will find the latest information on quality control, quality assurance, and laboratory design to help with running a safe, efficient molecular laboratory.

Key Features

· Presents the latest basic scientific theory underlying molecular diagnostics

· Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases

· Illustrates and summarizes key concepts and techniques with detailed figures and tables

· Advises on the latest quality control and quality assurance measures

· Explores emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks

Description

With its presentation of the components for using molecular techniques in the diagnostic laboratory, this book is certain to become a well-used standard. There has been a great deal of change in this area since the last edition was published 10 years ago.

Purpose

The book is a source of accurate information on the molecular techniques and practices needed to perform these tests in a diagnostic laboratory. This is an important goal at a time that more clinical laboratories are moving toward molecular testing methods.

Audience

It is designed for clinical laboratory personnel, including doctoral-level directors, pathologists, other physicians, and technical staff. The authors are all accomplished laboratory scientists with many years of experience in the field.

Features

Some of the detailed information this book presents on nucleic acid amplification techniques includes details on instruments and chemistries used to detect amplified product, dyes used for detection, how temperature is used in the process, and different types of probes. There is also an abundance of information on nucleic acid isolation. Several chapters include an appendix with resource information including databases. The largest section includes individual chapters on the detection of specific infectious agents such as monitoring the level of HIV in a patient before and during treatment, detection of methicillin resistant Staphylococcus aureus, and simultaneous detection of multiple respiratory viruses. Each of these chapters includes information on how this test is used in clinical medicine and tips on implementation. Another useful section is the last one, which gives guidance on controls and standards needed in each assay, verification studies, validation and continuous assessment of these diagnostic as ways. These are issues that continue to be of great importance to the development and performance of an accurate, useful diagnostic test.

Assessment

This book should be in any diagnostic laboratory that provides molecular tests for infectious agents. It contains valuable information that is difficult to find in one source.

Doody's Review Service

Reviewer: Rebecca Horvat, PhD, D(ABMM) (University of Kansas Medical Center)

Review Date: Unknown

©Doody’s Review Service

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