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Chapter 25 : PCR Detection of and

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Abstract:

This chapter outlines the need for, and application of, molecular tests for , , and , three sexually transmitted diseases (STDs) pathogens for which commercial molecular tests are not currently available. and are the causative agents of chancroid and syphilis, respectively, which along with herpes simplex virus (HSV) are responsible for the majority of genital ulcer disease (GUD) cases worldwide. The first PCR assays specific for were simultaneously developed in two laboratories and provided a means of detecting in patient specimens. The authors await further application of these quantitative PCR assays to assess other aspects of infection such as the association of burden in cervical, urine, and vaginal specimens and their association with signs and symptoms of infection at these sites. Unlike most PCRs, TMA assays target rRNA, which is present in multiple copies per cell, thus potentially increasing both the analytical and the clinical sensitivity of detection. Similarly, PCR and TMA assays for have allowed studies defining the association of this emerging pathogen with reproductive tract disease in both men and women. Undoubtedly other, previously uncultivated organisms colonizing the reproductive tract will be detected in the future. Their identification and the subsequent development of specific PCR assays and treatment regimens will expand one's knowledge of reproductive tract pathogens.

Citation: Totten P, Manhart L, Centurion-Lara A. 2011. PCR Detection of and , p 397-413. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch25

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Multiplex Real-Time PCR
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Reverse Transcriptase PCR
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Pelvic Inflammatory Disease
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Real-Time PCR
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Multiplex PCR
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FIGURE 1

PCR primer sets used for PCR assays, showing the different target sequences on the MgPa, 16S rRNA, and genes. References and sequences of these primer sets are listed in Table 2 , and their use in studies evaluating the association of with disease is shown in Table 3 .

Citation: Totten P, Manhart L, Centurion-Lara A. 2011. PCR Detection of and , p 397-413. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch25
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References

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Tables

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TABLE 1

Detection of and HSV DNA in genital ulcer specimens, showing differences in the relative prevalence of these three pathogens in different geographic settings and in the same setting in different years

Citation: Totten P, Manhart L, Centurion-Lara A. 2011. PCR Detection of and , p 397-413. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch25
Generic image for table
TABLE 2

Selected PCR primers and probes for

Citation: Totten P, Manhart L, Centurion-Lara A. 2011. PCR Detection of and , p 397-413. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch25
Generic image for table
TABLE 3

Selected studies assessing the association of with disease using the PCR assays outlined in Table 2 or an -specific TMA assay (Gen-Probe)

Citation: Totten P, Manhart L, Centurion-Lara A. 2011. PCR Detection of and , p 397-413. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch25

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