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Chapter 28 : Molecular Detection of Group B

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Abstract:

The advantage direct probe detection offers is a savings of 24 to 48 h in turnaround time (TAT) in detecting group B (GBS). Additionally because the assay is not an amplified molecular assay, there are no template contamination concerns when running the test. Conventional and real-time PCR assays have been developed for the rapid detection of GBS. GBS-PCR tests performed with alternative specimen types including amniotic fluid, neonatal screening swabs, blood, breast milk, urine, and serum have been described in the literature. A rapid and sensitive intrapartum real-time PCR assay offers the advantage of ascertaining the colonization status before delivery. A multiplex PCR-based reverse line-blot hybridization assay and partial sequence of gene has been used to compare the distribution of GBS serotypes, serotype III subtypes, and antibiotic resistance-associated genes. Determination of serotype is also epidemiologically important with respect to the proposed development of a preventative conjugate vaccine. Real-time PCR offers a powerful tool for sensitive, specific, and rapid detection of GBS directly from specimens at the time of delivery. It also offers a more sensitive antepartum test with improved TAT over culture.

Citation: Marlowe E, Pancholi P. 2011. Molecular Detection of Group B , p 453-462. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch28

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Real-Time PCR
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Figures

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FIGURE 1

The Gen-Probe AccuProbe Group B Streptococcus Culture Identification Test Kit. Courtesy Gen-Probe.

Citation: Marlowe E, Pancholi P. 2011. Molecular Detection of Group B , p 453-462. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch28
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FIGURE 2

The BD GeneOhm StrepB real-time PCR kit. This real-time PCR assay is run on the Cepheid SmartCycler. Courtesy BD Diagnostics.

Citation: Marlowe E, Pancholi P. 2011. Molecular Detection of Group B , p 453-462. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch28
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FIGURE 3

The Cepheid Smart GBS real-time PCR kit with the Cepheid SmartCycler. Courtesy Cepheid.

Citation: Marlowe E, Pancholi P. 2011. Molecular Detection of Group B , p 453-462. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch28
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FIGURE 4

The Cepheid Xpert GBS assay set up with the GeneXpert Dx System. Courtesy Cepheid.

Citation: Marlowe E, Pancholi P. 2011. Molecular Detection of Group B , p 453-462. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch28
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Tables

Generic image for table
TABLE 1

Collection and processing of GBS swabs for culture and susceptibility testing

Citation: Marlowe E, Pancholi P. 2011. Molecular Detection of Group B , p 453-462. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch28
Generic image for table
TABLE 2

Comparison of commercial molecular tests for GBS

Citation: Marlowe E, Pancholi P. 2011. Molecular Detection of Group B , p 453-462. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch28

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