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Chapter 39 : Molecular Detection of Respiratory Viruses
Category: Clinical Microbiology; Bacterial Pathogenesis
This chapter deals with current assays that are commercially available or noncommercial assays that have been well characterized in peer-reviewed literature. Methods for the molecular detection of respiratory viruses have, depending on the capabilities and resources available to the laboratory, a variety of potential clinical applications. Molecular amplification assays for the detection of rhinovirus RNA in clinical specimens often target the 5’ NCR, which contains sequences conserved across the 100 rhinovirus serotypes. In addition to advances in technology, another important step for the future will be the performance of clinical trials to prove the clinical usefulness of respiratory virus panel (RVP) results. Laboratorians and physicians will have to come to terms with co- and multiple infections. Molecular detection of respiratory viruses is currently a rapidly changing field and will likely continue to be for the near future. The good news is that more information about the many and varied causes of respiratory illness are currently acquired and that this information will be an important part of the puzzle in advancing human health.
Taxonomy of viruses causing human respiratory disease
Distribution of types among the human adenovirus species
Nucleic acid amplification assays for the detection of adenoviruses
Nucleic amplification assays for the detection of influenza viruses
Nucleic acid amplification assays for the detection of RSV
Nucleic acid amplification assays for the detection of HMPV