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Chapter 39 : Molecular Detection of Respiratory Viruses

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Abstract:

This chapter deals with current assays that are commercially available or noncommercial assays that have been well characterized in peer-reviewed literature. Methods for the molecular detection of respiratory viruses have, depending on the capabilities and resources available to the laboratory, a variety of potential clinical applications. Molecular amplification assays for the detection of rhinovirus RNA in clinical specimens often target the 5’ NCR, which contains sequences conserved across the 100 rhinovirus serotypes. In addition to advances in technology, another important step for the future will be the performance of clinical trials to prove the clinical usefulness of respiratory virus panel (RVP) results. Laboratorians and physicians will have to come to terms with co- and multiple infections. Molecular detection of respiratory viruses is currently a rapidly changing field and will likely continue to be for the near future. The good news is that more information about the many and varied causes of respiratory illness are currently acquired and that this information will be an important part of the puzzle in advancing human health.

Citation: Buller R, Arens M. 2011. Molecular Detection of Respiratory Viruses, p 604-630. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch39

Key Concept Ranking

Upper Respiratory Tract Infections
0.5453491
Lower Respiratory Tract Infections
0.54085356
Human respiratory syncytial virus
0.5403188
Human parainfluenza virus 1
0.48972037
Human parainfluenza virus 2
0.48972037
Human parainfluenza virus 4
0.48972037
0.5453491
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References

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Tables

Generic image for table
TABLE 1

Taxonomy of viruses causing human respiratory disease

Citation: Buller R, Arens M. 2011. Molecular Detection of Respiratory Viruses, p 604-630. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch39
Generic image for table
TABLE 2

Distribution of types among the human adenovirus species

Citation: Buller R, Arens M. 2011. Molecular Detection of Respiratory Viruses, p 604-630. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch39
Generic image for table
TABLE 3

Nucleic acid amplification assays for the detection of adenoviruses

Citation: Buller R, Arens M. 2011. Molecular Detection of Respiratory Viruses, p 604-630. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch39
Generic image for table
TABLE 4

Nucleic amplification assays for the detection of influenza viruses

Citation: Buller R, Arens M. 2011. Molecular Detection of Respiratory Viruses, p 604-630. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch39
Generic image for table
TABLE 5

Nucleic acid amplification assays for the detection of RSV

Citation: Buller R, Arens M. 2011. Molecular Detection of Respiratory Viruses, p 604-630. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch39
Generic image for table
TABLE 6

Nucleic acid amplification assays for the detection of HMPV

Citation: Buller R, Arens M. 2011. Molecular Detection of Respiratory Viruses, p 604-630. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch39

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