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Color Plates

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Figures

Image of COLOR PLATE 1 (chapter 1)

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COLOR PLATE 1 (chapter 1)

Multiplecolor diagram indicating the possible color combinations using three different fluorochromes. The combination of the three primary colors (red, green, and blue) results in four additional colors: pink (combination of red and blue), yellow (red and green), turquoise (blue and green), and white (all three colors). (Adapted from reference 177.)

Citation: Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A. 2011. Color Plates, In Molecular Microbiology. ASM Press, Washington, DC.
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Image of COLOR PLATE 2 (chapter 1)

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COLOR PLATE 2 (chapter 1)

Summary of the procedure for performing FISH on clinical samples.

Citation: Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A. 2011. Color Plates, In Molecular Microbiology. ASM Press, Washington, DC.
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Image of COLOR PLATE 3 (chapter 1)

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COLOR PLATE 3 (chapter 1)

Section of Whipple’s disease intestinal tissue sample. cells were detected with a Cy5-labeled, species-specific probe (blue). Cytoskeletal protein is colored in red, and cell nuclei are stained green due to uptake of YO-PRO 1 (Molecular Probes) nucleic acid stain. Magnification, ×400. (Reprinted from reference 45 with permission from University of Chicago Press.)

Citation: Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A. 2011. Color Plates, In Molecular Microbiology. ASM Press, Washington, DC.
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Image of COLOR PLATE 4 (chapter 1)

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COLOR PLATE 4 (chapter 1)

FISH of an artificial mixture of pure cultures from (red) and (not colored). Cells of appearing in the typical cord formation were detected with a Cy3-labeled oligonucleotide probe, specific for and . Note the specific fluorescent signals derived only from the mycobacterial cells and not from the surrounding cords or cells. The micrograph shows a superimposition of phase-contrast and fluorescence images.

Citation: Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A. 2011. Color Plates, In Molecular Microbiology. ASM Press, Washington, DC.
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Image of COLOR PLATE 5 (chapter 1)

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COLOR PLATE 5 (chapter 1)

Test of probe specificity and cross-reaction using three probes of different phylogenetic levels (genus, group, and species) for detection of four different species of in an artificial mixed culture. appears in blue, appears in turquoise, appears in white, and appears in pink.

Citation: Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A. 2011. Color Plates, In Molecular Microbiology. ASM Press, Washington, DC.
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Image of COLOR PLATE 6 (chapter 1)

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COLOR PLATE 6 (chapter 1)

In situ identification of by FISH directly applied on a nasopharyngeal smear from a 2-month-old girl. The yellow color is due to the imposition of two different labeled probes (Cy3 and fluorescein), a -specific probe (red), and one specific for the β-subclass of (green).

Citation: Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A. 2011. Color Plates, In Molecular Microbiology. ASM Press, Washington, DC.
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Image of COLOR PLATE 7 (chapter 1)

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COLOR PLATE 7 (chapter 1)

Cells of , directly detected in a bronchoalveolar lavage fluid sample with a species-specific probe labeled with Cy3 (red). Note the autofluorescent signals of the erythrocytes. Due to the superimposition of the images derived from the green and red channels, the red blood cells appear yellow and can be distinguished from the bacterial FISH signal.

Citation: Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A. 2011. Color Plates, In Molecular Microbiology. ASM Press, Washington, DC.
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Image of COLOR PLATE 8 (chapter 1)

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COLOR PLATE 8 (chapter 1)

Detection of branching, gram-positive rods derived from a clinical sample. A probe specific for the species was labeled with fluorescein and used for the identification of this microorganism.

Citation: Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A. 2011. Color Plates, In Molecular Microbiology. ASM Press, Washington, DC.
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Image of COLOR PLATE 9 (chapter 1)

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COLOR PLATE 9 (chapter 1)

In situ identification of directly in a blood sample. Diplococcoid cells of (green) were detected using a species-specific probe labeled with fluorescein. Images were taken from the red, green, and blue channels and superimposed, resulting in white autofluorescent erythrocytes because of the overlapping of the primary colors (Color Plate 1).

Citation: Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A. 2011. Color Plates, In Molecular Microbiology. ASM Press, Washington, DC.
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Image of COLOR PLATE 10 (chapter 1)

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COLOR PLATE 10 (chapter 1)

Microscope images of analyzed by PNA FISH using a -specific PNA probe (A) and a -specific PNA probe (B) and analyzed by PNA FISH with the PNA probe (C) and the PNA probe (D). (Reprinted from reference 125 with permission.)

Citation: Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A. 2011. Color Plates, In Molecular Microbiology. ASM Press, Washington, DC.
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Image of COLOR PLATE 11 (chapter 1)

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COLOR PLATE 11 (chapter 1)

FISH detection of pneumococci in a cerebrospinal fluid sample from a patient. The sample was stained by a -specific Cy3-labeled probe (A) in combination with the fluorescein isothiocyanate (FITC)-labeled eubacterial probe (B). As a further control, the DNA stain DAPI was implemented, which stained bacteria as well as the nucleus of a granulocyte (C). The overlay (D) demonstrates that the bacteria fluoresce in all channels, resulting in a white color, while the weak autofluorescence of the erythrocytes in the FITC and Cy3 channel results in a brown color. (Reprinted from reference 133 with permission.)

Citation: Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A. 2011. Color Plates, In Molecular Microbiology. ASM Press, Washington, DC.
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Image of COLOR PLATE 12 (chapter 42)

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COLOR PLATE 12 (chapter 42)

Example of an electrophoretogram obtained after multiplex PCR with reference strain B311 using three different microsatellite markers as described in reference 46. Two bands are obtained for each marker, as is diploid. The sizes of the PCR fragments (black, blue, and green peaks; one color per locus) are automatically calculated (GenScan software; Applied Biosystems) by using an internal standard (red peaks).

Citation: Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A. 2011. Color Plates, In Molecular Microbiology. ASM Press, Washington, DC.
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