Chapter 19 : Species

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Species, Page 1 of 2

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species cause one of the common opportunistic infections in immunocompromised persons. Due to lack of effective treatment, infection can cause prolonged and often life-threatening diarrhea in AIDS patients. The use of molecular biologic tools has made significant contributions to one's understanding of the biology and epidemiology of species. This chapter provides a snapshot of the genomes and discusses what has been learned about cryptosporidiosis from the genomic data. It discusses the key features of the genomes. used to be one of the genotype of but was renamed as a separate species a few years ago based largely on the host specificity, pathogenicity, and sequence differences from type II at various genomic loci. The genome shares an extremely high degree of similarity with that of . The major metabolic pathways encoded by the compact genome are highly streamlined by the absence of virtually all de novo synthetic capacities, such as those for amino acids, nucleotides, and fatty acids. The chapter deals with genomic data associated with host-pathogen interactions and pathogenesis, genomics and the development of molecular diagnostic and epidemiologic tools, and genome sequencing and public health. The lack of progress in research on the treatment of and vaccines against cryptosporidiosis is a particular public health concern. Better collaboration among molecular biologists, parasitologists, clinicians and other public health researchers, and the support of public health institutions, funding agencies, and policy decision makers will increase this progress.

Citation: Zhu G, Xiao L. 2011. Species, p 271-286. In Fratamico P, Liu Y, Kathariou S (ed), Genomes of Foodborne and Waterborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555816902.ch19

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Type II Fatty Acid Synthase
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Figure 1

Nucleotide sequence alignment of the two major copies of the partial ITS-1 (8611-8829), 5.8S (8830-8988, bold type), ITS-2 (8989-9545), and partial 28S (9546-9590, underlined) rRNA genes of The 5.8S gene of is identical to the sequence AAEL01000242.

Citation: Zhu G, Xiao L. 2011. Species, p 271-286. In Fratamico P, Liu Y, Kathariou S (ed), Genomes of Foodborne and Waterborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555816902.ch19
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1. Abrahamsen, M. S.,, T. J. Templeton,, S. Enomoto,, J. E. Abrahante,, G. Zhu,, C. A. Lancto,, M. Deng,, C. Liu,, G. Widmer,, S. Tzipori,, G. A. Buck,, P. Xu,, A. T. Bankier,, P. H. Dear,, B. A. Konfortov,, H. F. Spriggs,, L. Iyer,, V. Anantharaman,, L. Aravind, and, V. Kapur. 2004. Complete genome sequence of the apicom-plexan, Cryptosporidium parvum. Science 304:441445.
2. Alves, M.,, L. Xiao,, F. Antunes, and, O. Matos. 2006. Distribution of Cryptosporidium subtypes in humans and domestic and wild ruminants in Portugal. Parasitol. Res. 99:287292.
3. Alves, M.,, L. Xiao,, I. Sulaiman,, A. A. Lal,, O. Matos, and, F. Antunes. 2003. Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal. J. Clin. Microbiol. 41:27442747.
4. Anderson, A. C. 2005. Targeting DHFR in parasitic protozoa. Drug Discov. Today 10:121128.
5. Anderson, A. C. 2005. Two crystal structures of dihydrofolate reductase-thymidylate synthase from Cryptosporidium hominis reveal protein-ligand interactions including a structural basis for observed antifolate resistance. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 61:258262.
6. Arrowood, M. J. 2002. In vitro cultivation of Cryptosporidium species. Clin. Microbiol. Rev. 15:390400.
7. Artz, J. D.,, J. E. Dunford,, M. J. Arrowood,, A. Dong,, M. Chruszcz,, K. L. Kavanagh,, W. Minor,, R. G. Russell,, F. H. Ebetino,, U. Oppermann, and, R. Hui. 2008. Targeting a uniquely nonspecific prenyl synthase with bisphosphonates to combat cryptosporidiosis. Chem. Biol. 15:12961306.
8. Awad-el-Kariem, F. M.,, D. C. Warhurst, and, V. McDonald. 1994. Detection and species identification of Cryptosporidium oocysts using a system based on PCR and endonuclease restriction. Parasitology 109:1922.
9. Bankier, A. X,, H. F. Spriggs,, B. Fartmann,, B. A. Konfortov,, M. Madera,, C. Vogel,, S. A. Teichmann,, A. Ivens, and, P. H. Dear. 2003. Integrated mapping, chromosomal sequencing and sequence analysis of Cryptosporidium parvum. Genome Res. 13:17871799.
10. Barnes, D. A.,, A. Bonnin,, J. X. Huang,, L. Gousset,, J. Wu,, J. Gut,, P. Doyle,, J. F. Dubremetz,, H. Ward, and, C. Petersen. 1998. A novel multi-domain mucin-like glycoprotein of Cryptosporidium parvum mediates invasion. Mol. Biochem. Parasitol. 96:93110.
11. Barta, J. R., and, R. C. Thompson. 2006. What is Cryptosporidium? Reappraising its biology and phylogenetic affinities. Trends Parasitol. 22:463468.
12. Baum, J.,, D. Richard,, J. Healer,, M. Rug,, Z. Krnajski,, T. W. Gilberger,, J. L. Green,, A. A. Holder, and, A. F. Cowman. 2006. A conserved molecular motor drives cell invasion and gliding motility across malaria life cycle stages and other apicomplexan parasites. J. Biol. Chem. 281:51975208.
13. Bolstad, D. B.,, E. S. Bolstad,, K. M. Frey,, D. L. Wright, and, A. C. Anderson. 2008. Structure-based approach to the development of potent and selective inhibitors of dihydrofolate re-ductase from Cryptosporidium. J. Med. Chem. 51:68396852.
14. Bonnin, A.,, D. M. Ojcius,, P. Souque,, D. A. Barnes,, P. S. Doyle,, J. Gut,, R. G. Nelson,, C. Petersen, and, J. F. Dubremetz. 2001. Characterization of a monoclonal antibody reacting with antigen-4 domain of gp900 in Cryptosporidium parvum invasive stages. Parasitol. Res. 87:589592.
15. Bukhari, Z.,, M. M. Marshall,, D. G. Korich,, C. R. Fricker,, H. V. Smith,, J. Rosen, and, J. L. Clancy. 2000. Comparison of Cryptosporidium parvum viability and infectivity assays following ozone treatment of oocysts. Appl. Environ. Microbiol. 66:29722980.
16. Byington, C. L.,, R. L. Dunbrack,, Jr., F. G. Whitby,, F. E. Cohen, and, N. Agabian. 1997. Entamoeba histolytica: computer-assisted modeling of phosphofructokinase for the prediction of broad-spectrum antiparasitic agents. Exp. Parasitol. 87:194202.
17. Cama, V. A.,, M. J. Arrowood,, Y. R. Ortega, and, L. Xiao. 2006. Molecular characterization of the Cryptosporidium parvum IOWA isolate kept in different laboratories. J. Eu-karyot. Microbiol. 53:S40S42.
18. Cama, V. A.,, C. Bern,, J. Roberts,, L. Cabrera,, C. R. Sterling,, Y. Ortega,, R. H. Gilman, and, L. Xiao. 2008. Cryptosporidium species and subtypes and clinical manifestations in children, Peru. Emerg. Infect. Dis. 14:15671574.
19. Cama, V. A.,, J. M. Ross,, S. Crawford,, V. Kawai,, R. Chavez-Valdez,, D. Vargas,, A. Vivar,, E. Ticona,, M. Navincopa,, J. Williamson,, Y. Ortega,, R. H. Gilman,, C. Bern, and, L. Xiao. 2007. Differences in clinical manifestations among Cryptosporidium species and subtypes in HIV-infected persons. J. Infect. Dis. 196:684691.
20. Cevallos, A. M.,, X. Zhang,, M. K. Waldor,, S. Jaison,, X. Zhou,, S. Tzipori,, M. R. Neutra, and, H. D. Ward. 2000. Molecular cloning and expression of a gene encoding Cryptosporidium parvum glycoproteins gp40 and gp15. Infect. Immun. 68:41084116.
21. Chalmers, R. M.,, S. J. Hadfield,, C. J. Jackson,, K. Elwin,, L. Xiao, and, P. Hunter. 2008. Geographic linkage and variation in Cryptosporidium hominis. Emerg. Infect. Dis. 14:496498.
22. Chappell, C. L.,, P. C. Okhuysen,, C. R. Sterling, and, H. L. DuPont. 1996. Cryptosporidium parvum: intensity of infection and oocyst excretion patterns in healthy volunteers. J. Infect. Dis. 173:232236.
23. Chauret, C. P.,, C. Z. Radziminski,, M. Lepuil,, R. Creason, and, R. C. Andrews. 2001. Chlorine dioxide inactivation of Cryptosporidium parvum oocysts and bacterial spore indicators. Appl. Environ. Microbiol. 67:29933001.
24. Cohen, S.,, F. Dalle,, A. Gallay,, M. Di Palma,, A. Bonnin, and, H. D. Ward. 2006. Identification of Cpgp40/15 type Ib as the predominant allele in isolates of Cryptosporidium spp. from a waterborne outbreak of gastroenteritis in South Burgundy, France. J. Clin. Microbiol. 44:589591.
25. Coombs, G. H., and, S. Muller. 2002. Recent advances in the search for new anti-coccidial drugs. Int. J. Parasitol. 32:497508.
26. Coupe, S.,, C. Sarfati,, S. Hamane, and, F. Derouin. 2005. Detection of Cryptosporidium and identification to the species level by nested PCR and restriction fragment length polymorphism. J. Clin. Microbiol. 43:10171023.
27. Deng, M.,, T. J. Templeton,, N. R. London,, C. Bauer,, A. A. Schroeder, and, M. S. Abrahamsen. 2002. Cryptosporidium parvum genes containing thrombospondin type 1 domains. Infect. Immun. 70:69876995.
28. Dolo, A.,, D. Modiano,, O. Doumbo,, A. Bosman,, T. Sidibe,, M. M. Keita,, S. Naitza,, K. J. Robson, and, A. Crisanti. 1999. Thrombospondin related adhesive protein (TRAP), a potential malaria vaccine candidate. Parassitologia 41:425428.
29. Edward Martucci, W., M. Udier-Blagovic,, C. Atreya,, O. Babatunde,, M. A. Vargo,, W. L. Jorgensen, and, K. S. Anderson. 2009. Novel non-active site inhibitor of Cryptosporidium hominis TS-DHFR identified by a virtual screen. Bioorg. Med. Chem. Lett. 19:418423.
30. Ehigiator, H. N.,, N. McNair, and, J. R. Mead. 2003. IL-12 knockout C57BL/6 mice are protected from re-infection with Cryptosporidium parvum after challenge. J. Eukaryot. Micro-biol. 50(Suppl.):539541.
31. Ehigiator, H. N.,, P. Romagnoli,, J. W. Priest,, W. E. Secor, and, J. R. Mead. 2007. Induction of murine immune responses by DNA encoding a 23-kDa antigen of Cryptosporidium parvum. Parasitol. Res. 101:943950.
32. Entrala, E., and, C. Mascaro. 1997. Glycolytic enzyme activities in Cryptosporidium parvum oocysts. FEMS Microbiol. Lett. 151:5157.
33. Fayer, R. 2008. Introduction, p. 1–42. In R. Fayer and, L. Xiao (ed.), Cryptosporidium and Cryptosporidiosis, 2nd ed. CRC Press, Boca Raton, FL.
34. Feng, Y.,, T. Dearen,, V. Cama, and, L. Xiao. 2009. 90-kilodalton heat shock protein (hsp90) as a target for genotyping Cryptosporidium spp. known to infect humans. Eukaryot. Cell in press.
35. Finch, G. R.,, C. W. Daniels,, E. K. Black,, F. W. Schaefer III, and, M. Belosevic. 1993. Dose response of Cryptosporidium parvum in outbred neonatal CD-1 mice. Appl. Environ. Microbiol. 59:36613665.
36. Fritzler, J. M.,, J. J. Millership, and, G. Zhu. 2007. Cryptosporidium parvum long-chain fatty acid elongase. Eukaryot. Cell 6:20182028.
37. Galazka, J.,, B. Striepen, and, B. Ullman. 2006. Adenosine ki-nase from Cryptosporidium parvum. Mol. Biochem. Parasitol. 149:223230.
38. Gardner, M. J.,, R. Bishop,, T. Shah,, E. P. de Villiers,, J. M. Carlton,, N. Hall,, Q. Ren,, I. T. Paulsen,, A. Pain,, M. Berriman,, R. J. Wilson,, S. Sato,, S. A. Ralph,, D. J. Mann,, Z. Xiong,, S. J. Shallom,, J. Weidman,, L. Jiang,, J. Lynn,, B. Weaver,, A. Shoaibi,, A. R. Domingo,, D. Wasawo,, J. Crabtree,, J. R. Wortman,, B. Haas,, S. V. Angiuoli,, T. H. Creasy,, C. Lu,, B. Suh,, J. C. Silva,, T. R. Utterback,, T. V. Feldblyum,, M. Pertea,, J. Allen,, W. C. Nierman,, E. L. Taracha,, S. L. Salzberg,, O. R. White,, H. A. Fitzhugh,, S. Morzaria,, J. C. Venter,, C. M. Fraser, and, V. Nene. 2005. Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytes. Science 309:134137.
39. Gardner, M. J.,, N. Hall, E. Fung,, O. White,, M. Berriman,, R. W. Hyman,, J. M. Carlton,, A. Pain,, K. E. Nelson,, S. Bowman,, I. T. Paulsen,, K. James,, J. A. Eisen,, K. Rutherford,, S. L. Salzberg,, A. Craig,, S. Kyes,, M. S. Chan,, V. Nene,, S. J. Shallom,, B. Suh,, J. Peterson,, S. Angiuoli,, M. Pertea,, J. Allen,, J. Selengut,, D. Haft,, M. W. Mather,, A. B. Vaidya,, D. M. Martin,, A. H. Fairlamb,, M. J. Fraunholz,, D. S. Roos,, S. A. Ralph,, G. I. McFadden,, L. M. Cummings,, G. M. Subramanian,, C. Mungall,, J. C. Venter,, D. J. Carucci,, S. L. Hoffman,, C. Newbold,, R. W. Davis,, C. M. Fraser, and, B. Barrell. 2002. Genome sequence of the human malaria parasite Plasmodium falciparum. Nature 419:498511.
40. Gargala, G. 2008. Drug treatment and novel drug target against Cryptosporidium. Parasite (Paris) 15:275281.
41. Gasser, R. B., E. L. O. Y. G. Abs, S. Prepens, and, R. M. Chalmers. 2004. An improved ‘cold SSCP’ method for the genotypic and subgenotypic characterization of Cryptosporidium. Mol. Cell. Probes 18:329332.
42. Gasser, R. B.,, Y. G. El-Osta, and, R. M. Chalmers. 2003. Elec-trophoretic analysis of genetic variability within Cryptosporidium parvum from imported and autochthonous cases of human cryptosporidiosis in the United Kingdom. Appl. Environ. Microbiol. 69:27192730.
43. Gatei, W.,, D. Barrett,, J. F. Lindo,, D. Eldemire-Shearer, V. Cama, and, L. Xiao. 2008. Unique Cryptosporidium population in HIV-infected persons, Jamaica. Emerg. Infect. Dis. 14:841843.
44. Gatei, W.,, P. Das, P. Dutta,, A. Sen,, V. Cama,, A. A. Lal, and, L. Xiao. 2007. Multilocus sequence typing and genetic structure of Cryptosporidium hominis from children in Kolkata, India. Infect. Genet. Evol. 7:197205.
45. Gatei, W.,, C. A. Hart,, R. H. Gilman,, P. Das,, V. Cama, and, L. Xiao. 2006. Development of a multilocus sequence typing tool for Cryptosporidium hominis. J. Eukaryot. Microbiol. 53:S43S48.
46. Geurden, T.,, P. Thomas,, S. Casaert,, J. Vercruysse, and, E. Claerebout. 2008. Prevalence and molecular characterisation of Cryptosporidium and Giardia in lambs and goat kids in Belgium. Vet. Parasitol. 155:142145.
47. Gibbons-Matthews, C., and, A. M. Prescott. 2003. Intra-isolate variation of Cryptosporidium parvum small subunit ri-bosomal RNA genes from human hosts in England. Parasitol. Res. 90:439444.
48. Glaberman, S.,, J. E. Moore,, C. J. Lowery,, R. M. Chalmers,, I. Sulaiman,, K. Elwin,, P. J. Rooney,, B. C. Millar,, J. S. Dooley,, A. A. Lal, and, L. Xiao. 2002. Three drinking-water-associated cryptosporidiosis outbreaks, Northern Ireland. Emerg. Infect. Dis. 8:631633.
49. Gornicki, P. 2003. Apicoplast fatty acid biosynthesis as a target for medical intervention in apicomplexan parasites. Int. J. Parasitol. 33:885896.
50. Grinberg, A.,, N. Lopez-Villalobos,, W. Pomroy,, G. Widmer,, H. Smith, and, A. Tait. 2008. Host-shaped segregation of the Cryptosporidium parvum multilocus genotype repertoire. Epidemiol. Infect. 136:273278.
51. Huang, J.,, N. Mullapudi,, C. A. Lancto,, M. Scott,, M. S. Abrahamsen, and, J. C. Kissinger. 2004. Phylogenomic evidence supports past endosymbiosis, intracellular and horizontal gene transfer in Cryptosporidium parvum. Genome Biol. 5:R88.
52. Hunter, P. R., and, G. Nichols. 2002. Epidemiology and clinical features of Cryptosporidium infection in immunocompromised patients. Clin. Microbiol. Rev. 15:145154.
53. Jelenska, J.,, M. J. Crawford,, O. S. Harb,, E. Zuther,, R. Haselkorn,, D. S. Roos, and, P. Gornicki. 2001. Subcellular localization of acetyl-CoA carboxylase in the apicomplexan parasite Toxoplasma gondii. Proc. Natl. Acad. Sci. USA 98:27232728.
54. Jelenska, J.,, A. Sirikhachornkit,, R. Haselkorn, and, P. Gornicki. 2002. The carboxyltransferase activity of the apicoplast acetyl-CoA carboxylase of Toxoplasma gondii is the target of aryloxyphenoxypropionate inhibitors. J. Biol. Chem. 277:2320823215.
55. Jellison, K. L.,, H. F. Hemond, and, D. B. Schauer. 2002. Sources and species of Cryptosporidium oocysts in the wachusett reservoir watershed. Appl. Environ. Microbiol. 68:569575.
56. Jex, A. R.,, A. Pangasa,, B. E. Campbell,, M. Whipp,, G. Hogg,, M. I. Sinclair,, M. Stevens, and, R. B. Gasser. 2008. Classification of Cryptosporidium from sporadic cryptosporidiosis cases in humans employing sequence-based multilocus analysis following mutation scanning. J. Clin. Microbiol. 46:22522262.
57. Jex, A. R.,, M. Whipp,, B. E. Campbell,, S. M. Caccio,, M. Stevens,, G. Hogg, and, R. B. Gasser. 2007. A practical and cost-effective mutation scanning-based approach for investigating genetic variation in Cryptosporidium. Electrophoresis 28:38753883.
58. Jiang, J., and, L. Xiao. 2003. An evaluation of molecular diagnostic tools for the detection and differentiation of human-pathogenic Cryptosporidium spp. J. Eukaryot. Microbiol. 50 Suppl:542547.
59. Kairo, A.,, A. H. Fairlamb,, E. Gobright, and, V. Nene. 1994. A 7.1 kb linear DNA molecule of Theileria parva has scrambled rDNA sequences and open reading frames for mitochondrially encoded proteins. EMBO J. 13:898905.
60. Kappe, S. H.,, C. A. Buscaglia, and, V. Nussenzweig. 2004. Plasmodium sporozoite molecular cell biology. Annu. Rev. Cell Dev. Biol. 20:2959.
61. Kappe, S. H.,, K. Kaiser, and, K. Matuschewski. 2003. The Plasmodium sporozoite journey: a rite of passage. Trends Parasitol. 19:135143.
62. Kim, K., and, L. M. Weiss. 2004. Toxoplasma gondii: the model apicomplexan. Int. J. Parasitol. 34:423432.
63. Kimbell, L. M.,, D. L. Miller,, W. Chavez, and, N. Altman. 1999. Molecular analysis of the 18S rRNA gene of Cryptosporidium serpentis in a wild-caught corn snake (Elaphe guttata guttata) and a five-species restriction fragment length polymorphism-based assay that can additionally discern C. parvum from C. wrairi. Appl. Environ. Microbiol. 65:53455349.
64. Korich, D. G.,, M. M. Marshall,, H. V. Smith,, J. O’Grady,, Z. Bukhari,, C. R. Fricker,, J. P. Rosen, and, J. L. Clancy. 2000. Inter-laboratory comparison of the CD-1 neonatal mouse logistic dose-response model for Cryptosporidium parvum oocysts. J. Eukaryot. Microbiol. 47:294298.
65. Korich, D. G.,, J. R. Mead,, M. S. Madore,, N. A. Sinclair, and, C. R. Sterling. 1990. Effects of ozone, chlorine dioxide, chlorine, and monochloramine on Cryptosporidium parvum oocyst viability. Appl. Environ. Microbiol. 56:14231428.
66. LaGier, M. J.,, J. Tachezy,, F. Stejskal,, K. Kutisova, and, J. S. Keithly. 2003. Mitochondrial-type iron-sulfur cluster biosynthesis genes (IscS and IscU) in the apicomplexan Cryptosporidium parvum. Microbiology (Reading) 149:35193530.
67. Le Blancq, S. M.,, N. V. Khramtsov,, F. Zamani,, S. J. Upton, and, T. W. Wu. 1997. Ribosomal RNA gene organization in Cryptosporidium parvum. Mol. Biochem. Parasitol. 90:463478.
68. Leng, X.,, D. A. Mosier, and, R. D. Oberst. 1996. Differentiation of Cryptosporidium parvum, C. muris, and C. baileyi by PCR-RFLP analysis of the 18S rRNA gene. Vet. Parasitol. 62:17.
69. Leoni, F.,, M. E. Mallon,, H. V. Smith,, A. Tait, and, J. McLauchlin. 2007. Multilocus analysis of Cryptosporidium hominis and Cryptosporidium parvum from sporadic and outbreak-related human cases and C. parvum from sporadic cases in livestock in the UK. J. Clin. Microbiol. 45:32863294.
70. Liu, J.,, D. B. Bolstad,, E. S. D. Bolstad,, D. L. Wright, and, A. C. Anderson. 2009. Towards new antifolates targeting eukaryotic opportunistic infections. Eukaryot. Cell 8:483486.
71. Lowery, C. J.,, J. E. Moore,, B. C. Millar,, D. P. Burke,, K. A. McCorry,, E. Crothers, and, J. S. Dooley. 2000. Detection and speciation of Cryptosporidium spp. in environmental water samples by immunomagnetic separation, PCR and endonu-clease restriction. J. Med. Microbiol. 49:779785.
72. Mac Kenzie, W. R.,, N. J. Hoxie,, M. E. Proctor,, M. S. Gradus,, K. A. Blair,, D. E. Peterson,, J. J. Kazmierczak,, D. G. Addiss,, K. R. Fox, and, J. B. Rose. 1994. A massive outbreak in Milwaukee of Cryptosporidium infection transmitted through the public water supply. N. Engl. J. Med. 331:161167.
73. Madern, D.,, X. Cai,, M. S. Abrahamsen, and, G. Zhu. 2004. Evolution of Cryptosporidium parvum lactate dehydrogenase from malate dehydrogenase by a very recent event of gene duplication. Mol. Biol. Evol. 21:489497.
74. Madrid-Aliste, C. J.,, J. M. Dybas,, R. Hogue Angeletti,, L. M. Weiss,, K. Kim,, I. Simon, and, A. Fiser. 2009. EPIC-DB: a pro-teomics database for studying Apicomplexan organisms. BMC Genomics 10:38.
75. Mallon, M.,, A. MacLeod,, J. Wastling,, H. Smith,, B. Reilly, and, A. Tait. 2003. Population structures and the role of genetic exchange in the zoonotic pathogen Cryptosporidium parvum. J. Mol. Evol. 56:407417.
76. Mallon, M. E.,, A. MacLeod,, J. M. Wastling,, H. Smith, and, A. Tait. 2003. Multilocus genotyping of Cryptosporidium parvum Type 2: population genetics and sub-structuring. Infect. Genet. Evol. 3:207218.
77. Martucci, W. E.,, M. A. Vargo, and, K. S. Anderson. 2008. Explaining an unusually fast parasitic enzyme: folate tail-binding residues dictate substrate positioning and catalysis in Cryptosporidium hominis thymidylate synthase. Biochemistry 47:89028911.
78. Michalski, W. P.,, J. A. Edgar, and, S. J. Prowse. 1992. Mannitol metabolism in Eimeria tenella. Int. J. Parasitol. 22:11571163.
79. Morgan-Ryan, U. M.,, A. Fall,, L. A. Ward,, N. Hijjawi,, I. Sulaiman,, R. Fayer,, R. C. Thompson,, M. Olson,, A. Lal, and, L. Xiao. 2002. Cryptosporidium hominis n. sp. (Apicomplexa: Cryptosporidiidae) from Homo sapiens. J. Eukaryot. Microbiol. 49:433440.
80. Morgan, U. M.,, C. C. Constantine,, P. O’Donoghue,, B. P. Meloni,, P. A. O’Brien, and, R. C. Thompson. 1995. Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis. Am. J. Trop. Med. Hyg. 52:559564.
81. Morgan, U. M.,, P. Deplazes,, D. A. Forbes,, F. Spano,, H. Hertzberg,, K. D. Sargent,, A. Elliot, and, R. C. Thompson. 1999. Sequence and PCR-RFLP analysis of the internal transcribed spacers of the rDNA repeat unit in isolates of Cryptosporidium from different hosts. Parasitology 118:4958.
82. Morrison, L. J.,, M. E. Mallon,, H. V. Smith,, A. Macleod,, L. Xiao, and, A. Tait. 2008. The population structure of the Cryptosporidium parvum population in Scotland: a complex picture. Infect. Genet. Evol. 8:121129.
83. Ngouanesavanh, T.,, K. Guyot,, G. Certad,, Y. L. Fichoux,, C. Chartier,, R. -I. Verdier,, J. -C. Cailliez,, D. Camus,, E. Dei-Cas, and, A. -L. Banuls. 2006. Cryptosporidium population genetics: Evidence of clonality in isolates from France and Haiti. J. Eukaryot. Microbiol. 53:S33S36.
84. Nichols, R. A.,, B. M. Campbell, and, H. V. Smith. 2003. Identification of Cryptosporidium spp. oocysts in United Kingdom noncarbonated natural mineral waters and drinking waters by using a modified nested PCR-restriction fragment length polymorphism assay. Appl. Environ. Microbiol. 69:41834189.
85. Nime, F. A.,, J. D. Burek,, D. L. Page,, M. A. Holscher, and, J. H. Yardley. 1976. Acute enterocolitis in a human being infected with the protozoan Cryptosporidium. Gastroenterology 70:592598.
86. Novak, S. M., and, C. R. Sterling. 1991. Susceptibility dynamics in neonatal BALB/c mice infected with Cryptosporidium parvum. J. Protozool. 38:103S104S.
87. Obornik, M.,, J. Janouskovec,, T. Chrudimsky, and, J. Lukes. 2009. Evolution of the apicoplast and its hosts: from het-erotrophy to autotrophy and back again. Int. J. Parasitol. 39:112.
88. O’Brien, E.,, L. McInnes, and, U. Ryan. 2008. Cryptosporidium GP60 genotypes from humans and domesticated animals in Australia, North America and Europe. Exp. Parasitol. 118:118121.
89. Pain, A.,, H. Renauld,, M. Berriman,, L. Murphy,, C. A. Yeats,, W. Weir,, A. Kerhornou,, M. Aslett,, R. Bishop,, C. Bouchier,, M. Cochet,, R. M. Coulson,, A. Cronin,, E. P. de Villiers,, A. Fraser,, N. Fosker,, M. Gardner,, A. Goble,, S. Griffiths-Jones,, D. E. Harris,, F. Katzer,, N. Larke,, A. Lord,, P. Maser,, S. McKellar,, P. Mooney,, F. Morton,, V. Nene,, S. O’Neil,, C. Price,, M. A. Quail,, E. Rabbinowitsch,, N. D. Rawlings,, S. Rutter,, D. Saunders,, K. Seeger,, T. Shah,, R. Squares,, S. Squares,, A. Tivey,, A. R. Walker,, J. Woodward,, D. A. Dobbelaere,, G. Langsley,, M. A. Rajandream,, D. McKeever,, B. Shiels,, A. Tait,, B. Barrell, and, N. Hall. 2005. Genome of the host-cell transforming parasite Theileria annulata compared with T. parva. Science 309:131133.
90. Pangasa, A.,, A. R. Jex,, B. E. Campbell,, N. J. Bott,, M. Whipp,, G. Hogg,, M. A. Stevens, and, R. B. Gasser. 2009. High resolution melting-curve (HRM) analysis for the diagnosis of cryptosporidiosis in humans. Mol. Cell. Probes 23:1015.
91. Perkins, M. E.,, Y. A. Riojas,, T. W. Wu, and, S. M. Le Blancq. 1999. CpABC, a Cryptosporidium parvum ATP-binding cassette protein at the host-parasite boundary in intracellular stages. Proc. Natl. Acad. Sci. USA 96:57345739.
92. Pohlenz, J.,, W. J. Bemrick,, H. W. Moon, and, N. F. Cheville. 1978. Bovine cryptosporidiosis: a transmission and scanning electron microscopic study of some stages in the life cycle and of the host-parasite relationship. Vet. Pathol. 15:417427.
93. Popov, V. M.,, D. C. Chan,, Y. A. Fillingham,, W. Atom Yee,, D. L. Wright, and, A. C. Anderson. 2006. Analysis of complexes of inhibitors with Cryptosporidium hominis DHFR leads to a new trimethoprim derivative. Bioorg. Med. Chem. Lett. 16:43664370.
94. Priest, J. W.,, J. P. Kwon,, M. J. Arrowood, and, P. J. Lammie. 2000. Cloning of the immunodominant 17-kDa antigen from Cryptosporidium parvum. Mol. Biochem. Parasitol. 106:261271.
95. Quilez, J.,, E. Torres,, R. M. Chalmers,, S. J. Hadfield,, E. Del Cacho, and, C. Sanchez-Acedo. 2008. Genotype and subtype characterization of Cryptosporidium in lambs and goat kids in Spain. Appl. Environ. Microbiol. 74:60266031.
96. Riordan, C. E.,, J. G. Ault,, S. G. Langreth, and, J. S. Keithly. 2003. Cryptosporidium parvum Cpn60 targets a relict or-ganelle. Curr. Genet. 44:138147.
97. Roberts, C. W.,, F. Roberts,, F. L. Henriquez,, D. Akiyoshi,, B. U. Samuel,, T. A. Richards,, W. Milhous,, D. Kyle,, L. McIntosh,, G. C. Hill,, M. Chaudhuri,, S. Tzipori, and, R. McLeod. 2004. Evidence for mitochondrial-derived alternative oxidase in the apicomplexan parasite Cryptosporidium parvum: a potential anti-microbial agent target. Int. J. Parasitol. 34:297308.
98. Rochelle, P. A.,, M. M. Marshall,, J. R. Mead,, A. M. Johnson,, D. G. Korich,, J. S. Rosen, and, R. De Leon. 2002. Comparison of In Vitro cell culture and a mouse assay for measuring infectivity of Cryptosporidium parvum. Appl. Environ. Microbiol. 68:38093817.
99. Rotte, C.,, F. Stejskal,, G. Zhu,, J. S. Keithly, and, W. Martin. 2001. Pyruvate : NADP+ oxidoreductase from the mitochondrion of Euglena gracilis and from the apicomplexan Cryptosporidium parvum: a biochemical relic linking pyruvate metabolism in mitochondriate and amitochondriate protists. Mol. Biol. Evol. 18:710720.
100. Schindler, A. R.,, Y. G. Abs El-Osta,, M. Stevens,, M. I. Sinclair, and, R. B. Gasser. 2005. Capillary electrophoretic analysis of fragment length polymorphism in ribosomal markers of Cryptosporidium from humans. Mol. Cell. Probes 19:394399.
101. Schmatz, D. M. 1989. The mannitol cycle-a new metabolic pathway in the Coccidia. Parasitol. Today 5:205208.
102. Schnittger, L.,, P. Shayan,, R. Biermann,, H. Mehlhorn,, J. Gerdes, and, J. S. Ahmed. 2000. Molecular genetic characterization and subcellular localization of Theileria annulata mitochondrial heat-shock protein 70. Parasitol. Res. 86:444452.
103. Schnyder, M.,, L. Kohler,, A. Hemphill, and, P. Deplazes. 2009. Prophylactic and therapeutic efficacy of nitazoxanide against Cryptosporidium parvum in experimentally challenged neonatal calves. Vet. Parasitol. 160:149154.
104. Slapeta, J., and, J. S. Keithly. 2004. Cryptosporidium parvum mitochondrial-type HSP70 targets homologous and heter-ologous mitochondria. Eukaryot. Cell. 3:483494.
105. Striepen, B., and, J. C. Kissinger. 2004. Genomics meets transgenics in search of the elusive Cryptosporidium drug target. Trends Parasitol. 20:355358.
106. Striepen, B.,, A. J. Pruijssers,, J. Huang,, C. Li,, M. J. Gubbels,, N. N. Umejiego,, L. Hedstrom, and, J. C. Kissinger. 2004. Gene transfer in the evolution of parasite nucleotide biosynthesis. Proc. Natl. Acad. Sci. USA 101:31543159.
107. Strong, W. B., and, R. G. Nelson. 2000. Gene discovery in Cryptosporidium parvum: expressed sequence tags and genome survey sequences. Contrib. Microbiol. 6:92115.
108. Strong, W. B., and, R. G. Nelson. 2000. Preliminary profile of the Cryptosporidium parvum genome: an expressed sequence tag and genome survey sequence analysis. Mol. Biochem. Parasitol. 107:132.
109. Sturbaum, G. D.,, C. Reed,, P. J. Hoover,, B. H. Jost,, M. M. Marshall, and, C. R. Sterling. 2001. Species-specific, nested PCR-restriction fragment length polymorphism detection of single Cryptosporidium parvum oocysts. Appl. Environ. Microbiol. 67:26652668.
110. Sulaiman, I. M.,, L. Xiao, and, A. A. Lal. 1999. Evaluation of Cryptosporidium parvum genotyping techniques. Appl. Environ. Microbiol. 65:44314435.
111. Sultan, A. A.,, V. Thathy,, U. Frevert,, K. J. Robson,, A. Crisanti,, V. Nussenzweig,, R. S. Nussenzweig, and, R. Menard. 1997. TRAP is necessary for gliding motility and infectivity of plas-modium sporozoites. Cell 90:511522.
112. Suzuki, X, T. Hashimoto,, Y. Yabu,, Y. Kido,, K. Sakamoto,, C. Nihei,, M. Hato,, S. Suzuki,, Y. Amano,, K. Nagai,, T. Hosokawa,, N. Minagawa,, N. Ohta, and, K. Kita. 2004. Direct evidence for cyanide-insensitive quinol oxidase (alternative oxidase) in apicomplexan parasite Cryptosporidium parvum: phylogenetic and therapeutic implications. Biochem. Biophys. Res. Comm. 313:10441052.
113. Taghi-Kilani, R.,, M. Remacha-Moreno, and, W. M. Wenman. 1994. Three tandemly repeated 5S ribosomal RNA-encoding genes identified, cloned and characterized from Cryptosporidium parvum. Gene 142:253258.
114. Tanriverdi, S.,, J. C. Blain,, B. Deng,, M. T. Ferdig, and, G. Widmer. 2007. Genetic crosses in the apicomplexan parasite Cryptosporidium parvum define recombination parameters. Mol. Microbiol. 63:14321439.
115. Tanriverdi, S.,, A. Grinberg,, R. M. Chalmers,, P. R. Hunter,, Z. Petrovic,, D. E. Akiyoshi,, E. London,, L. Zhang,, S. Tzipori,, J. K. Tumwine, and, G. Widmer. 2008. Inferences about the global population structure of Cryptosporidium parvum and Cryptosporidium hominis. Appl. Environ. Microbiol. 74:72277234.
116. Tanriverdi, S.,, A. Markovics,, M. O. Arslan,, A. Itik,, V. Shkap, and, G. Widmer. 2006. Emergence of distinct genotypes of Cryptosporidium parvum in structured host populations. Appl. Environ. Microbiol. 72:25072513.
117. Tanriverdi, S., and, G. Widmer. 2006. Differential evolution of repetitive sequences in Cryptosporidium parvum and Cryptosporidium hominis. Infect. Genet. Evol. 6:113122.
118. Templeton, T. J.,, L. M. Iyer,, V. Anantharaman,, S. Enomoto,, J. E. Abrahante,, G. M. Subramanian,, S. L. Hoffman,, M. S. Abrahamsen, and, L. Aravind. 2004. Comparative analysis of apicomplexa and genomic diversity in eukaryotes. Genome Res. 14:16861695.
119. Thompson, R. C.,, M. E. Olson,, G. Zhu,, S. Enomoto,, M. S. Abrahamsen, and, N. S. Hijjawi. 2005. Cryptosporidium and cryptosporidiosis. Adv. Parasitol. 59:77158.
120. Tyzzer, E. 1910. An extracelluar coccidium, Cryptosporidium muris (gen. & sp. nov.), of the gastric glands of the common mouse. J. Med. Res. 18:487509.
121. Tyzzer, E. 1907. A sporozoon found in the peptic glands of the common mouse. Proc. Soc. Exp. Biol. Med. 5:1213.
122. Tyzzer, E. E. 1912. Cryptosporidium parvum (sp. nov.), a coccidium found in the small intestine of the common mouse. Arch. Protisenkd. 26:394412.
123. Umejiego, N. N.,, D. Gollapalli,, L. Sharling,, A. Volftsun,, J. Lu,, N. N. Benjamin,, A. H. Stroupe,, T. V. Riera,, B. Striepen, and, L. Hedstrom. 2008. Targeting a prokaryotic protein in a eukaryotic pathogen: identification of lead compounds against cryptosporidiosis. Chem. Biol. 15:7077.
124. Umejiego, N. N.,, C. Li,, T. Riera,, L. Hedstrom, and, B. Striepen. 2004. Cryptosporidium parvum IMP dehydrogenase: identification of functional, structural, and dynamic properties that can be exploited for drug design. J. Biol. Chem. 279:4032040327.
125. Vasquez, J. R.,, L. Gooze,, K. Kim,, J. Gut,, C. Petersen, and, R. G. Nelson. 1996. Potential antifolate resistance determinants and genotypic variation in the bifunctional dihydrofolate reductase-thymidylate synthase gene from human and bovine isolates of Cryptosporidium parvum. Mol. Biochem. Parasitol. 79:153165.
126. Viel, H.,, H. Rocques,, J. Martin, and, C. Chartier. 2007. Efficacy of nitazoxanide against experimental cryptosporidiosis in goat neonates. Parasitol. Res. 102:163166.
127. Waller, R. F., and, G. I. McFadden. 2005. The apicoplast: a review of the derived plastid of apicomplexan parasites. Curr. Issues Mol. Biol. 7:5779.
128. Wengelnik, K.,, R. Spaccapelo,, S. Naitza,, K. J. Robson,, C. J. Janse,, F. Bistoni,, A. P. Waters, and, A. Crisanti. 1999. The A-domain and the thrombospondin-related motif of Plasmodium falciparum TRAP are implicated in the invasion process of mosquito salivary glands. EMBO J. 18:51955204.
129. Widmer, G. 2004. Population genetics of Cryptosporidium parvum. Trends Parasitol. 20:36.
130. Wielinga, P. R.,, A. de Vries,, T. H. van der Goot,, T. Mank,, M. H. Mars,, L. M. Kortbeek, and, J. W. van der Giessen. 2008. Molecular epidemiology of Cryptosporidium in humans and cattle in The Netherlands. Int. J. Parasitol. 38:809817.
131. Xiao, L.,, C. Bern,, J. Limor,, I. Sulaiman,, J. Roberts,, W. Checkley,, L. Cabrera,, R. H. Gilman, and, A. A. Lal. 2001. Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru. J. Infect. Dis. 183:492497.
132. Xiao, L.,, L. Escalante,, C. Yang,, I. Sulaiman,, A. A. Escalante,, R. J. Montali,, R. Fayer, and, A. A. Lal. 1999. Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus. Appl. Environ. Microbiol. 65:15781583.
133. Xiao, L., and, R. Fayer. 2008. Molecular characterisation of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission. Int. J. Parasitol. 38:12391255.
134. Xiao, L., and, Y. Feng. 2008. Zoonotic cryptosporidiosis. FEMS Immunol. Med. Microbiol. 52:309323.
135. Xiao, L.,, J. R. Limor,, L. Li,, U. Morgan,, R. C. Thompson, and, A. A. Lal. 1999. Presence of heterogeneous copies of the small subunit rRNA gene in Cryptosporidium parvum human and marsupial genotypes and Cryptosporidium felis. J. Eukaryot. Microbiol. 46:44S45S.
136. Xiao, L., and, U. M. Ryan. 2008. Molecular epidemiology, p. 119-171. In R. Fayer and, L. Xiao (ed.), Cryptosporidium and Cryptosporidiosis, 2nd ed. CRC Press, Boca Raton, FL.
137. Xu, P.,, G. Widmer,, Y. Wang,, L. S. Ozaki,, J. M. Alves,, M. G. Serrano,, D. Puiu,, P. Manque,, D. Akiyoshi,, A. J. Mackey,, W. R. Pearson,, P. H. Dear,, A. T. Bankier,, D. L. Peterson,, M. S. Abrahamsen,, V. Kapur,, S. Tzipori, and, G. A. Buck. 2004. The genome of Cryptosporidium hominis. Nature 431:11071112.
138. Zapata, F.,, M. E. Perkins,, Y. A. Riojas,, T. W. Wu, and, S. M. Le Blancq. 2002. The Cryptosporidium parvum ABC protein family. Mol. Biochem. Parasitol. 120:157161.
139. Zeng, B.,, X. Cai, and, G. Zhu. 2006. Functional characterization of a fatty acyl-CoA-binding protein (ACBP) from the apicomplexan Cryptosporidium parvum. Microbiology (Reading) 152:23552363.
140. Zhou, R.,, G. Li,, S. Xiao,, Y. Xia, and, Y. Guo. 2007. PCR amplification and sequence analyses of ITS-1 rDNA from Cryptosporidium andersoni in dairy cattle. Parasitol. Res. 100:11351138.
141. Zhu, G. 2008. Biochemistry, p. 57-77. In R. Fayer and, L. Xiao (ed.), Cryptosporidium and Cryptosporidiosis, 2nd ed. CRC Press, Boca Raton, FL.
142. Zhu, G. 2004. Current progress in the fatty acid metabolism in Cryptosporidium parvum. J. Eukaryot. Microbiol. 51:381388.
143. Zhu, G., and, J. S. Keithly. 2002. Alpha-proteobacterial relationship of apicomplexan lactate and malate dehydrogenases. J. Eukaryot. Microbiol. 49:255261.
144. Zhu, G.,, J. S. Keithly, and, H. Philippe. 2000. What is the phylogenetic position of Cryptosporidium? Int. J. Syst. Evol. Microbiol. 50:16731681.
145. Zhu, G.,, M. J. LaGier,, F. Stejskal,, J. J. Millership,, X. Cai, and, J. S. Keithly. 2002. Cryptosporidium parvum: the first protist known to encode a putative polyketide synthase. Gene 298:7989.
146. Zhu, G.,, Y. Li,, X. Cai,, J. J. Millership,, M. J. Marchewka, and, J. S. Keithly. 2004. Expression and functional characterization of a giant Type I fatty acid synthase (CpFAS1) gene from Cryptosporidium parvum. Mol. Biochem. Parasitol. 134:127135.
147. Zhu, G.,, M. J. Marchewka, and, J. S. Keithly. 2000. Cryptosporidium parvum appears to lack a plastid genome. Microbiology (Reading) 146:315321.
148. Zhu, G.,, M. J. Marchewka,, K. M. Woods,, S. J. Upton, and, J. S. Keithly. 2000. Molecular analysis of a Type I fatty acid synthase in Cryptosporidium parvum. Mol. Biochem. Parasitol. 105:253260.
149. Zintl, A.,, A. F. Proctor,, C. Read,, T. Dewaal,, N. Shanaghy,, S. Fanning, and, G. Mulcahy. 2009. The prevalence of Cryptosporidium species and subtypes in human faecal samples in Ireland. Epidemiol. Infect. 137:270277.


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Table 1

Major features of the genome in comparison with other apicomplexans

Citation: Zhu G, Xiao L. 2011. Species, p 271-286. In Fratamico P, Liu Y, Kathariou S (ed), Genomes of Foodborne and Waterborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555816902.ch19

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