Chapter 25 : Spatiotemporal Organization of Multispecies Communities in the Human Oral Microbiome

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Spatiotemporal Organization of Multispecies Communities in the Human Oral Microbiome, Page 1 of 2

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To control multispecies community function within dental plaque, one must first understand the spatiotemporal organization of the species within the community. Oral multispecies communities are not randomly organized. This chapter illustrates the application of metatranscriptomics to in vitro biofilm communities constructed with defined species. It describes the application of a sensitive procedure called catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) that measures mRNA at the single-cell level in vivo. A strategy to use the species to investigate metatranscriptomics of their communities, is presented in the chapter. Metatranscriptomic analysis of this community could document some gene expression changes accompanying this burst of growth. In summary, it appears that each of the three initial colonizers favors interaction with specific partners: - sp., -, and -. With the availability of numerous genome-sequenced strains from the Human Oral Microbiome initiative, great progress can be achieved through metatranscriptomic analyses of two-, three-, and four-species biofilms growing on saliva as the sole source of nutrition. By using genome-sequenced strains, assigning gene functions to cDNA sequences will be much easier, and rapid progress can be made in understanding the relationship of community membership, spatiotemporal organization, and multispecies community growth.

Citation: Palmer, Jr. R, Kolenbrander P. 2011. Spatiotemporal Organization of Multispecies Communities in the Human Oral Microbiome, p 371-381. In Kolenbrander P (ed), Oral Microbial Communities. ASM Press, Washington, DC. doi: 10.1128/9781555817107.ch25

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Streptococcus oralis
Streptococcus gordonii
Streptococcus anginosus
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Two-species and three-species biofilm communities growing on saliva as the sole nutritional source

Citation: Palmer, Jr. R, Kolenbrander P. 2011. Spatiotemporal Organization of Multispecies Communities in the Human Oral Microbiome, p 371-381. In Kolenbrander P (ed), Oral Microbial Communities. ASM Press, Washington, DC. doi: 10.1128/9781555817107.ch25

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