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Chapter 14 : in Pork

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Abstract:

Foodborne bacteria transmitted to humans via contaminated pork have a major impact, especially in developed countries. Among these bacteria, has been characterized by one of the highest scores of risk for pork consumers. Consumption of pork has been associated with infections in epidemiological studies. Raw pork products have been widely investigated due to the link between and pigs. The serological analysis method can be used for the estimation of the prevalence of in pig herds. The method has an advantage further down in the meat chain, since infection of pigs during transport and in the lairage will not interfere with the results. Even cross-contamination during slaughtering and dressing will not affect the result. Not only blood but also muscle fluid can be used as the basis for the analysis. Real-time PCR is a powerful advancement of the basic PCR technique. A future challenge for sample preparation is to design PCR protocols that integrate DNA extraction and amplification in an automated manner. Additionally, high-throughput technologies such as DNA-microarray, mass spectrometry-PCR, and whole-genome sequencing platforms will strengthen the portfolio of molecular diagnostic tools for pathogenic .

Citation: Fredriksson-Ahomaa M, Nesbakken T, Skurnik M, Thisted Lambertz S, Dickson J, Hoorfar J, Korkeala H. 2011. in Pork, p 195-207. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch14
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Figures

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FIGURE 1

Occurrence of bioserotype 4/O:3 in samples of feces and tonsils, and occurrence of antibodies against O:3 in blood samples, from different age groups of pigs from the two herds investigated by Nesbakken et al. ( ). Adapted from Lium et al. ( ) and Nesbakken (2008). *, numbers of animals differ since feces, tonsils, or blood samples were not tested from all animals due to unsuccessful sampling from some animals. Reprinted from the , p. 399 ( ), with permission from the publisher.

Citation: Fredriksson-Ahomaa M, Nesbakken T, Skurnik M, Thisted Lambertz S, Dickson J, Hoorfar J, Korkeala H. 2011. in Pork, p 195-207. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch14
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References

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Tables

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TABLE 1

Sampling and testing based on the most significant preventive measures to minimize spread of from slaughter pigs, via meat and meat products, to humans

Citation: Fredriksson-Ahomaa M, Nesbakken T, Skurnik M, Thisted Lambertz S, Dickson J, Hoorfar J, Korkeala H. 2011. in Pork, p 195-207. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch14
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TABLE 2

PCR methods for detection of pathogenic in pork samples

Citation: Fredriksson-Ahomaa M, Nesbakken T, Skurnik M, Thisted Lambertz S, Dickson J, Hoorfar J, Korkeala H. 2011. in Pork, p 195-207. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch14
Generic image for table
TABLE 3

Primers and probe used for detection of -positive by real-time PCR

Citation: Fredriksson-Ahomaa M, Nesbakken T, Skurnik M, Thisted Lambertz S, Dickson J, Hoorfar J, Korkeala H. 2011. in Pork, p 195-207. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch14
Generic image for table
TABLE 4

Pipetting scheme for the TaqMan real-time PCR assay

Citation: Fredriksson-Ahomaa M, Nesbakken T, Skurnik M, Thisted Lambertz S, Dickson J, Hoorfar J, Korkeala H. 2011. in Pork, p 195-207. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch14
Generic image for table
TABLE 5

PCR cycling parameters for the TaqMan real-time PCR assay

Citation: Fredriksson-Ahomaa M, Nesbakken T, Skurnik M, Thisted Lambertz S, Dickson J, Hoorfar J, Korkeala H. 2011. in Pork, p 195-207. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch14

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