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Chapter 14 : in Pork

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Abstract:

Foodborne bacteria transmitted to humans via contaminated pork have a major impact, especially in developed countries. Among these bacteria, has been characterized by one of the highest scores of risk for pork consumers. Consumption of pork has been associated with infections in epidemiological studies. Raw pork products have been widely investigated due to the link between and pigs. The serological analysis method can be used for the estimation of the prevalence of in pig herds. The method has an advantage further down in the meat chain, since infection of pigs during transport and in the lairage will not interfere with the results. Even cross-contamination during slaughtering and dressing will not affect the result. Not only blood but also muscle fluid can be used as the basis for the analysis. Real-time PCR is a powerful advancement of the basic PCR technique. A future challenge for sample preparation is to design PCR protocols that integrate DNA extraction and amplification in an automated manner. Additionally, high-throughput technologies such as DNA-microarray, mass spectrometry-PCR, and whole-genome sequencing platforms will strengthen the portfolio of molecular diagnostic tools for pathogenic .

Citation: Fredriksson-Ahomaa M, Nesbakken T, Skurnik M, Thisted Lambertz S, Dickson J, Hoorfar J, Korkeala H. 2011. in Pork, p 195-207. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch14
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FIGURE 1

Occurrence of bioserotype 4/O:3 in samples of feces and tonsils, and occurrence of antibodies against O:3 in blood samples, from different age groups of pigs from the two herds investigated by Nesbakken et al. ( ). Adapted from Lium et al. ( ) and Nesbakken (2008). *, numbers of animals differ since feces, tonsils, or blood samples were not tested from all animals due to unsuccessful sampling from some animals. Reprinted from the , p. 399 ( ), with permission from the publisher.

Citation: Fredriksson-Ahomaa M, Nesbakken T, Skurnik M, Thisted Lambertz S, Dickson J, Hoorfar J, Korkeala H. 2011. in Pork, p 195-207. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch14
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References

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1. Anonymous. 1998. Pathogenic Yersinia enterocolitica. PCR Methods for Detection in Foods. NMKL Method NO. 163. Nordic Committee on Food Analysis, Oslo, Norway.
2. Bhaduri, S.,, and I. Wesley. 2006. Isolation and characterization of Yersinia enterocolitica from swine feces recovered during the National Animal Health Monitoring System Swine 2000 Study. J. Food Prot. 69:21072112.
3. Boqvist, S.,, H. Pettersson,, A. Svensson, and, Y. Andersson. 2009. Sources of sporadic Yersinia enterocolitica infection in children in Sweden, 2004: a case-control study. Epidemiol. Infect. 137:897905.
4. Boyapalle, S.,, I. V. Wesley,, H. S. Hurd, and, P. G. Reddy. 2001. Comparison of culture, multiplex, and 5′ nuclease polymerase chain reaction assay for the rapid detection of Yersinia enterocolitica in swine and pork products. J. Food Prot. 64:13521361.
5. Commission of the European Community. 2001. Commission decision of 8 June 2001. Off. J. Eur. Commun. L 165:853.
6. Eggenberger-Solorzano, L.,, S. E. Niebuhr,, G. R. Acuff, and, J. S. Dickson. 2002. Hot water and organic acid interventions to control microbiological contamination on hog carcasses during processing. J. Food Prot. 65:12481252.
7. European Food Safety Authority. 2009. Technical specification for harmonized national surveys on Yersinia enterocolitica in slaughter pigs. EFSA J. 7:13741397.
8. Fosse, J.,, H. Seegers, and, C. Magars. 2009. Prevalence and risk factors for bacterial food-borne zoonotic hazards in slaughter pigs: a review. Zoonosis Public Health 56:429454.
9. Fredriksson-Ahomaa, M.,, and H. Korkeala. 2003. Low occurrence of pathogenic Yersinia enterocolitica in clinical, food, and environmental samples: a methodological problem. Clin. Microbiol. Rev. 16:220229.
10. Fredriksson-Ahomaa, M.,, M. Lindström, and, H. Korkeala. 2010. Yersinia enterocolitica and Yersinia pseudotuberculosis, p. 164–180. In V. K. Juneja and, N. J. Sofos (ed.), Pathogens and Toxins in Foods: Challenges and Interventions. ASM Press, Washington, DC.
11. Fredriksson-Ahomaa, M.,, A. Stolle, and, H. Korkeala. 2006. Molecular epidemiology of Yersinia enterocolitica infections. FEMS Immunol. Med. Microbiol. 47:315329.
12. Fricker, M.,, U. Messelhausser,, U. Busch,, S. Scherer, and, M. Ehling-Schulz. 2007. Diagnostic real-time PCR assays for the detection of emetic Bacillus cereus strains in foods and recent food-borne outbreaks. Appl. Environ. Microbiol. 73:18921898.
13. Gierczynski, R.,, A. Golubov,, H. Neubauer,, J. N. Pham, and, A. Rakin. 2007. Development of multiple-locus variable-number tandem-repeat analysis for Yersinia enterocolitica subsp. palearctica and its application to bioserogroup 4/O:3 subtyping. J. Clin. Microbiol. 45:25082515.
14. Hallanvuo, S.,, J. Peltola,, T. Heiskanen, and, A. Siitonen. 2006. Simplified phenotypic scheme evaluated by 16S rRNA sequencing for differentiation between Yersinia enterocolitica and Y. enterocolitica-like species. J. Clin. Microbiol. 44:10771080.
15. Hoorfar, J.,, A. Wedderkopp, and, P. Lind. 1997. Detection of antibodies to Salmonella lipopolysaccharides in muscle fluid from cattle. Am. J. Vet. Res. 58:334337.
16. Hudson, J. A.,, N. J. King,, A. J. Cornelius,, T. Bigwood,, K. Thom, and, S. Monson. 2008. Detection, isolation and enumeration of Yersinia enterocolitica from raw pork. Int. J. Food Microbiol. 123:2531.
17. Jourdan, A. D.,, S. C. Johnson, and, I. V. Wesley. 2000. Development of a fluorogenic 5′ nuclease PCR assay for detection of the ail gene of pathogenic Yersinia enterocolitica. Appl. Environ. Microbiol. 66:37503755.
18. Knutsson, R.,, M. Fontanesi,, H. Grage, and, P. Rådström. 2002. Development of a PCR-compatible enrichment medium for Yersinia enterocolitica: amplification precision and dynamic detection range during cultivation. Int. J. Food Microbiol. 72:185201.
19. Lambertz, S. T.,, C. Nilsson,, S. Hallanvuo, and, M. Lindblad. 2008. Real-time PCR method for detection of pathogenic Yersinia enterocolitica in food. Appl. Environ. Microbiol. 74:60606067.
20. Laukkanen, R.,, P. O. Martínez,, K.-M. Siekkinen,, J. Ranta,, R. Maijala, and, H. Korkeala. 2009. Contamination of carcasse with human pathogenic Yersinia enterocolitica 4/O:3 originates from pigs infected on farms. Foodborne Pathog. Dis. 6:681688.
21. Lindblad, M.,, H. Lindmark,, S. T. Lambertz, and, R. Lindqvist. 2007. Microbiological baseline study of swine carcasses at Swedish slaughterhouses. J. Food Prot. 70:17901797.
22. Lium, B.,, T. Iversen,, K. Eckner, and, T. Nesbakken. 2006. Prevalence of Yersinia enterocolitica O:3 in different age groups of pigs in naturally infected integrated herds, p. 388. Proceedings of the 19th International Pig Veterinary Society Congress, 2006, Copenhagen.
23. Martínez, P. O.,, S. Mylona,, I. Drake,, M. Fredriksson-Ahomaa,, H. Korkeala, and, J. Corry. 2010. Wide variety of bioserotypes of enteropathogenic Yersinia in tonsils of English pigs at slaughter. Int. J. Food Microbiol. 139:6469.
24. Nesbakken, T. 2009. Control of human pathogenic Yersinia enterocolitica in the meat chain. Thesis. Norwegian School of Veterinary Science, Oslo, Norway.
25. Nesbakken, T.,, K. Eckner,, H. K. Høidal, and, O. J. Røtterud. 2003. Occurrence of Yersinia enterocolitica and Campylobacter spp. in slaughter pigs and consequences for meat inspection, slaughtering and dressing procedures. Int. J. Food Microbiol. 80:231240.
26. Nesbakken, T.,, T. Iversen,, K. Eckner, and, B. Lium. 2006. Testing of pathogenic Yersinia enterocolitica in pig herds based on the natural dynamic of infection. Int. J. Food Microbiol. 111:99104.
27. Nielsen, B.,, C. Heisel, and, A. Wingstrand. 1996. Time course of the serological response to Yersinia enterocolitica O:3 in experimentally infected pigs. Vet. Microbiol. 48:293303.
28. Nissen, H.,, O. Alvseike,, S. Bredholt,, A. Hock, and, T. Nesbakken. 2000. Comparison between the growth of Yersinia enterocolitica, Listeria monocytogenes and Escherichia coli O157:H7 and Salmonella spp. in ground beef packed by three commercially used packaging techniques. Int. J. Food Microbiol. 59:211220.
29. Shiozawa, K.,, T. Nishina,, Y. Miwa,, T. Mori,, S. Akahane, and, K. Ito. 1991. Colonization in the tonsils of swine by Yersinia enterocolitica. Contrib. Microbiol. Immunol. 12:6367.
30. Siddique, N.,, D. Sharma, and, S. F. Khaldi. 2009. Detection of Yersinia enterocolitica in alfalfa, mung bean, cilantro, and mamey sapota food matrices using DNA microarray chip hybridization. Curr. Microbiol. 59:233239.
31. Skjerve, E.,, B. Lium,, B. Nielsen, and, T. Nesbakken. 1998. Control of Yersinia enterocolitica in pigs at herd level. Int. J. Food Microbiol. 45:195203.
32. Skurnik, M.,, P. Rådström,, R. Knutsson,, B. Segerman,, S. Hallanvuo,, S. Thisted Lambertz,, H. Korkeala, and, M. Fredriksson-Ahomaa. 2009. Yersinia, p. 501–518. In D. Liu (ed.), Molecular Detection of Foodborne Pathogens. CRC Press, Boca Raton, FL.
33. Vishnubhatla, A.,, R. D. Oberst,, D. Y. C. Fung,, W. Wonglumson,, M. P. Hays, and, T. G. Nagaraja. 2001. Evaluation of a 5′-nuclease (TaqMan) assay for the detection of virulent strains of Yersinia enterocolitica in raw meat and tofu samples. J. Food Prot. 64:355360.
34. Wauters, G.,, V. Goossens,, M. Janssens, and, J. Vandepitte. 1988. New enrichment method for isolation of pathogenic Yersinia enterocolitica serogroup O:3 from pork. Appl. Environ. Microbiol. 54:851854.
35. Weynants, V.,, A. Tibor,, P. A. Denoel,, C. Saegerman,, J. Godfroid,, P. Thiange, and, J.-J. Letesson. 1996. Infection of cattle with Yersinia enterocolitica O:9 a cause of the false positive serological reactions in bovine brucellosis diagnostic tests. Vet. Microbiol. 48:101112.

Tables

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TABLE 1

Sampling and testing based on the most significant preventive measures to minimize spread of from slaughter pigs, via meat and meat products, to humans

Citation: Fredriksson-Ahomaa M, Nesbakken T, Skurnik M, Thisted Lambertz S, Dickson J, Hoorfar J, Korkeala H. 2011. in Pork, p 195-207. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch14
Generic image for table
TABLE 2

PCR methods for detection of pathogenic in pork samples

Citation: Fredriksson-Ahomaa M, Nesbakken T, Skurnik M, Thisted Lambertz S, Dickson J, Hoorfar J, Korkeala H. 2011. in Pork, p 195-207. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch14
Generic image for table
TABLE 3

Primers and probe used for detection of -positive by real-time PCR

Citation: Fredriksson-Ahomaa M, Nesbakken T, Skurnik M, Thisted Lambertz S, Dickson J, Hoorfar J, Korkeala H. 2011. in Pork, p 195-207. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch14
Generic image for table
TABLE 4

Pipetting scheme for the TaqMan real-time PCR assay

Citation: Fredriksson-Ahomaa M, Nesbakken T, Skurnik M, Thisted Lambertz S, Dickson J, Hoorfar J, Korkeala H. 2011. in Pork, p 195-207. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch14
Generic image for table
TABLE 5

PCR cycling parameters for the TaqMan real-time PCR assay

Citation: Fredriksson-Ahomaa M, Nesbakken T, Skurnik M, Thisted Lambertz S, Dickson J, Hoorfar J, Korkeala H. 2011. in Pork, p 195-207. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch14

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