Chapter 17 : Encounters with Mammalian Cells: Survival Strategies of Species

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This chapter reviews the changes in cellular physiology that follow contact with host cells and the impact of these changes on the host-pathogen interaction. The focus is on , for the simple reason that the vast majority of the published research is on this species. The chapter focuses on the interaction between cells and the most important and relevant mammalian cell types. Reflecting the scope of available literature, the chapter concentrates on interactions with neutrophils and macrophages. Stress responses include reactive oxygen species (ROS), reactive nitrogen species (RNS) and superoxide dismutases (SODs). The chapter focuses on the effectors and their roles in mediating the interaction with host cells. Invasion and endocytosis are terms primarily used to describe the internalization of into normally nonphagocytic cells, mostly endothelial and epithelial cells. A surprising finding from the analysis of macrophagephagocytosed cells was the role of carbon starvation, which represented nearly two-thirds of the genes whose expression changed. The adhesion between cells and phagocytes is primarily driven by receptors on the mammalian cell that recognize carbohydrate moieties on the cell wall, though there are some fungal proteins that mediate adhesion as well.

Citation: Vylkova S, Lorenz M. 2012. Encounters with Mammalian Cells: Survival Strategies of Species, p 261-282. In Calderone R, Clancy C (ed), and Candidiasis, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817176.ch17

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Tumor Necrosis Factor alpha
Innate Immune System
Fungal Proteins
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Extracellular detoxification of ROS. Depicted are a generic phagocyte producing superoxide, hydrogen peroxide, and NO, opposed with a cell elaborating cell surface and intracellular antioxidant mechanisms (see symbol key). doi:10.1128/9781555817176.ch17.f1

Citation: Vylkova S, Lorenz M. 2012. Encounters with Mammalian Cells: Survival Strategies of Species, p 261-282. In Calderone R, Clancy C (ed), and Candidiasis, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817176.ch17
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Mechanisms by which can cross endothelial layers. (A) Endocytosis can be triggered by specific interactions between ALS3 (triangles) and N-cadherin (“Y”), which leads to pseudopod formation and internalization. Hyphal morphogenesis inside the endothelial cell can lead to rupture and escape. (B) Passage between cells by degrading extracellular matrix and tight junctions. Scissors represent secreted proteases. (C) Passage through the host cell. Scissors represent secreted proteases and/or lipases. (D) Transit within phagocytes. A phagocytosed cell crosses the endothelial layer within a migrating phagocyte. Many of these mechanisms are also applicable to interactions with epithelial cells; see reference for greater detail on host epithelial interactions. doi:10.1128/9781555817176.ch17.f2

Citation: Vylkova S, Lorenz M. 2012. Encounters with Mammalian Cells: Survival Strategies of Species, p 261-282. In Calderone R, Clancy C (ed), and Candidiasis, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817176.ch17
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morphogenesis and escape following macrophage phagocytosis. A wild-type strain expressing a constitutive ACT1 promoter-GFP construct was incubated with J774A.1 macrophages for the indicated times. Fluorescent images are overlaid onto the phase image to highlight the fungal cells. doi:10.1128/9781555817176.ch17.f3

Citation: Vylkova S, Lorenz M. 2012. Encounters with Mammalian Cells: Survival Strategies of Species, p 261-282. In Calderone R, Clancy C (ed), and Candidiasis, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817176.ch17
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