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Chapter 11 : Procedures for the Storage of Microorganisms*
Preservation of microorganisms for future study has a long tradition in microbiology. Culture collections of microorganisms are valuable resources for scientific research in microbial diversity and evolution, patient care management, epidemiological investigations, and educational purposes. Preserved individual strains of microorganisms serve as permanent records of microorganisms’ unique phenotypic profiles and provide the material for further genotypic characterizations. Effective storage involves maintaining an organism in a viable state free of contamination and without changes in its genotypic or phenotypic characteristics. The organism must also be easily restored to its condition prior to preservation. This chapter presents methods that can be used for the storage of bacteria, protozoa, fungi, and viruses.