1887

Chapter 11 : Procedures for the Storage of Microorganisms*

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $30.00

Preview this chapter:
Zoom in
Zoomout

Procedures for the Storage of Microorganisms*, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555817381/9781555817381.ch11-1.gif /docserver/preview/fulltext/10.1128/9781555817381/9781555817381.ch11-2.gif

Abstract:

Preservation of microorganisms for future study has a long tradition in microbiology. Culture collections of microorganisms are valuable resources for scientific research in microbial diversity and evolution, patient care management, epidemiological investigations, and educational purposes. Preserved individual strains of microorganisms serve as permanent records of microorganisms’ unique phenotypic profiles and provide the material for further genotypic characterizations. Effective storage involves maintaining an organism in a viable state free of contamination and without changes in its genotypic or phenotypic characteristics. The organism must also be easily restored to its condition prior to preservation. This chapter presents methods that can be used for the storage of bacteria, protozoa, fungi, and viruses.

Citation: She R, Petti C. 2015. Procedures for the Storage of Microorganisms*, p 161-168. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch11
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

References

/content/book/10.1128/9781555817381.mcm11.ch11
1. Prudden T. 1887. On bacteria in ice, and their relations to disease, with special reference to the ice-supply of New York City. Med Rec 31:341378.
2. Swift HF. 1921. Preservation of stock cultures of bacteria by freezing and drying. J Exp Med 33:6975.
3. Alexander M,, Daggett PM,, Gherna R,, Jong J,, Simione F. 1980. American Type Culture Collection Methods, vol. I. Laboratory Manual on Preservation, Freezing, and Freeze-Drying as Applied to Algae, Bacteria, Fungi and Protozoa, p 146. American Type Culture Collection, Rockville, MD.
4. Gherna RL,. 1981. Preservation, p 208217. In Gerhardt P,, Murray RGE,, Costilow RN,, Nester EN,, Wood WA,, Krieg NR,, Phillips GB (ed), Manual of Methods for General Bacteriology. American Society for Microbiology, Washington, DC.
5. Heckly RJ. 1961. Preservation of bacteria by lyophilization. Adv Appl Microbiol 3:176.
6. Heckly RJ. 1978. Preservation of microorganisms. Adv Appl Microbiol 24:153.
7. Smith D,, Onions AHS. 1994. The Preservation and Maintenance of Living Fungi, 2nd ed. CAB International, Wallingford, Oxon, United Kingdom.
8. Lynch M,, Sung W,, Morris K,, Coffey N,, Landry CR,, Dopman EB,, Dickinson WJ,, Okamoto K,, Kulkarni S,, Hartl DL,, Thomas WK. 2008. A genome-wide view of the spectrum of spontaneous mutations in yeast. Proc Natl Acad Sci U S A 105:92729277.
9. Lee H,, Popodi E,, Tang H,, Foster PL. 2012. Rate and molecular spectrum of spontaneous mutations in the bacterium Escherichia coli as determined by whole-genome sequencing. Proc Natl Acad Sci U S A 109:E2774E2783.
10. Clinical and Laboratory Standards Institute. 2012. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard9th Edition. CLSI Document M07-A9. Clinical Laboratory and Standards Institute, Wayne, PA.
11. Harbec PS,, Turcotte P. 1996. Preservation of Neisseria gonorrhoeae at –20 degrees C. J Clin Microbiol 34:11431146.
12. Jackson H. 1974. Loss of viability and metabolic injury of Staphylococcus aureus resulting from storage at 5 degrees C. J Appl Bacteriol 37:5964.
13. Miyamoto-Shinohara Y,, Imaizumi T,, Sukenobe J,, Murakami Y,, Kawamura S,, Komatsu Y. 2000. Survival rate of microbes after freeze-drying and long-term storage. Cryobiology 41:251255.
14. McGinnis MR,, Padhye AA,, Ajello L. 1974. Storage of stock cultures of filamentous fungi, yeasts, and some aerobic actinomycetes in sterile distilled water. Appl Microbiol 28:218222.
15. van Griethuysen A,, van Loo I,, van Belkum A,, Vandenbroucke-Grauls C,, Wannet W,, van Keulen P,, Kluytmans J. 2005. Loss of the mecA gene during storage of methicillin-resistant Staphylococcus aureus strains. J Clin Microbiol 43:13611365.
16. Pell PA,, Sneath PH. 1984. A note on the survival of bacteria in cryoprotectant medium at temperatures above 0 degrees C. J Appl Bacteriol 57:165167.
17. Hubalek Z. 2003. Protectants used in the cryopreservation of microorganisms. Cryobiology 46:205229.
18. Feltham RK,, Power AK,, Pell PA,, Sneath PA. 1978. A simple method for storage of bacteria at –76 degrees C. J Appl Bacteriol 44:313316.
19. Miyamoto-Shinohara Y,, Sukenobe J,, Imaizumi T,, Nakahara T. 2008. Survival of freeze-dried bacteria. J Gen Appl Microbiol 54:924.
20. Barbaree JM,, Sanchez A. 1982. Cross-contamination during lyophilization. Cryobiology 19:443447.
21. Borman AM,, Linton CJ,, Miles SJ,, Campbell CK,, Johnson EM. 2006. Ultra-rapid preparation of total genomic DNA from isolates of yeast and mould using Whatman FTA filter paper technology—a reusable DNA archiving system. Med Mycol 44:389398.
22. Rajendram D,, Ayenza R,, Holder FM,, Moran B,, Long T,, Shah HN. 2006. Long-term storage and safe retrieval of DNA from microorganisms for molecular analysis using FTA matrix cards. J Microbiol Methods 67:582592.
23. Das PC,, de Vries AH,, McShine RL,, Sibinga CT. 1996. Dried sera for confirming blood-borne virus infections (HCV, HTLV-I, HIV & HBsAg). Transfus Med 6:319323.
24. Cassol S,, Salas T,, Gill MJ,, Montpetit M,, Rudnik J,, Sy CT,, O’Shaughnessy MV. 1992. Stability of dried blood spot specimens for detection of human immunodeficiency virus DNA by polymerase chain reaction. J Clin Microbiol 30:30393042.
25. Lloyd RM,, Burns DA,, Huong JT,, Mathis RL,, Winters MA,, Tanner M,, De La Rosa A,, Yen-Lieberman B,, Armstrong W,, Taege A,, McClernon DR,, Wetshtein JL,, Friedrich BM,, Ferguson MR,, O’Brien W,, Feorino PM,, Holodniy M. 2009. Dried-plasma transport using a novel matrix and collection system for human immunodeficiency virus and hepatitis C virus virologic testing. J Clin Microbiol 47:14911496.
26. Phetsouvanh R,, Blacksell SD,, Jenjaroen K,, Day NPJ,, Newton PN. 2009. Comparison of indirect immunofluorescence assays for diagnosis of scrub typhus and murine typhus using venous blood and finger prick filter paper blood spots. Am J Trop Med Hyg 80:837840.
27. Popovic T,, Ajello G,, Facklam R. 1999. Laboratory Manual for the Diagnosis of Meningitis Caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae. World Health Organization, Geneva, Switzerland.
28. Siberry G,, Brahmadathan KN,, Pandian R,, Lalitha MK,, Steinhoff MC,, John TJ. 2001. Comparison of different culture media and storage temperatures for the long-term preservation of Streptococcus pneumoniae in the tropics. Bull World Health Organ 79:4347.
29. Votava M,, Stritecka M. 2001. Preservation of Haemophilus influenzae and Haemophilus parainfluenzae at –70 degrees C. Cryobiology 43:8587.
30. Miyata A. 1975. Cryo-preservation of the parasitic protozoa. Jap J Trop Med Hyg 3:161200.
31. Crespo MJ,, Abarca ML,, Cabanes FJ. 2000. Evaluation of different preservation and storage methods for Malassezia spp. J Clin Microbiol 38:38723875.
32. Taddei A,, Tremarias MM,, Hartung de Capriles C. 19981999. Viability studies on actinomycetes. Mycopathologia 143:161164.
33. Qiangqiang Z,, Jiajun W,, Li L. 1998. Storage of fungi using sterile distilled water or lyophilization: comparison after 12 years. Mycoses 41:255257.
34. Castellani A. 1939. Viability of some pathogenic fungi in distilled water. J Trop Med Hyg 42:225226.
35. Hartung de Capriles C,, Mata S,, Middelveen M. 1989. Preservation of fungi in water (Castellani): 20 years. Mycopathologia 106:7379.
36. Antheunisse J,, de Bruin-Tol JW,, van der Pol-van Soest ME. 1981. Survival of microorganisms after drying and storage. Antonie Van Leeuwenhoek 47:539545.
37. Rybnikar A. 1995. Long-term maintenance of lyophilized fungal cultures of the genera Epidermophyton, Microsporum, Paecilomyces and Trichophyton. Mycoses 38:145147.
38. Ellis JJ,, Roberson JA. 1968. Viability of fungus cultures preserved by lyophilization. Mycologia 60:399405.
39. Johnson FB. 1990. Transport of viral specimens. Clin Microbiol Rev 3:120131.
40. Law TJ,, Hull RN. 1968. The stabilizing effect of sucrose upon respiratory syncytial virus infectivity. Proc Soc Exp Biol Med 128:515518.
41. Williams FP. 1989. Electron microscopy of stool-shed viruses: retention of characteristic morphologies after long-term storage at ultralow temperatures. J Med Virol 29:192195.
42. Gallo D,, Kimpton JS,, Johnson PJ. 1989. Isolation of human immunodeficiency virus from peripheral blood lymphocytes stored in various transport media and frozen at –60 degrees C. J Clin Microbiol 27:8890.
43. Gould EA. 1999. Methods for long-term virus preservation. Mol Biotechnol 13:5766.
44. Tannock GA,, Hierholzer JC,, Bryce DA,, Chee CF,, Paul JA. 1987. Freeze-drying of respiratory syncytial viruses for transportation and storage. J Clin Microbiol 25:17691771.
45. Federal Register. 2005. Possession, use, and transfer of select agents and toxins, final rule. Fed Regist 70:1329413325.
46. Cody WL,, Wilson JW,, Hendrixson DR,, McIver KS,, Hagman KE,, Ott CM,, Nickerson CA,, Schurr MJ. 2008. Skim milk enhances the preservation of thawed –80 degrees C bacterial stocks. J Microbiol Methods 75:135138.
47. DePaoli P. 2005. Bio-banking in microbiology: from sample collection to epidemiology, diagnosis, and research. FEMS Microbiol Rev 29:897910.
48. Elliott P,, Peakman TC,, UK Biobank. 2008. The UK Biobank sample handling and storage protocol for the collection, processing and archiving of human blood and urine. Int J Epidemiol 37:234244.

Tables

Generic image for table
TABLE 1

Common procedures for preservation of microorganisms

Citation: She R, Petti C. 2015. Procedures for the Storage of Microorganisms*, p 161-168. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch11

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error