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Chapter 115 : Reagents, Stains, and Media: Mycology*

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Reagents, Stains, and Media: Mycology*, Page 1 of 2

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Abstract:

This chapter describes the reagents, stains, and media that are available and commonly used for the identification of fungi, ranging from direct detection of fungal structures in patient specimens to selection of media for the culture and identification of fungal isolates.

Citation: Lindsley M, Snyder J, Atlas R, Larocco M. 2015. Reagents, Stains, and Media: Mycology*, p 1955-1964. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch115
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References

/content/book/10.1128/9781555817381.mcm11.ch115
1. Dismukes WE,, Pappas PG,, Sobel JD. 2003. Clinical Mycology. Oxford University Press, Inc, New York, NY.
2. Larone DH. 2011. Medically Important Fungi, 5th ed. ASM Press, Washington, DC.
3. McGinnis M. 1980. Laboratory Handbook of Medical Mycology. Academic Press, New York, NY.
4. Atlas RM,, Snyder JW. 2006. Handbook of Media for Clinical Microbiology, 2nd ed. Taylor & Francis, Boca Raton, FL.
5. Ellis D,, Davis S,, Alexiou H,, Handke R,, Bartley R. 2007. Descriptions of Medical Fungi, 2nd ed. Mycology Unit, Women's and Children's Hospital, North Adelaide, South Australia, Australia.
6. Leslie JF,, Summerell BA. 2006. The Fusarium Laboratory Manual. Blackwell Publishing, Ames, IA.
7. Clinical and Laboratory Standards Institute. 2012. Principles and Procedures for Detection of Fungi in Clinical Specimens—Direct Examination and Culture. Standard M54-A. Clinical and Laboratory Standards Institute, Wayne, PA.
8. National Committee for Clinical Laboratory Standards. 2004. Quality Control for Commercially Prepared Microbiological Culture Media. Standard M22-A3. National Committee for Clinical Laboratory Standards, Wayne, PA.
9. Hageage GJ,, Harrington BJ. 1984. Use of calcofluor white in clinical mycology. Lab Med 15:109112.
10. Kim YK,, Parulekar S,, Yu PK,, Pisani RJ,, Smith TF,, Anhalt JP. 1990. Evaluation of calcofluor white stain for detection of Pneumocystis carinii. Diagn Microbiol Infect Dis 13:307310.
11. Paradis IL,, Ross C,, Dekker A,, Dauber J. 1990. A comparison of modified methenamine silver and toluidine blue stains for the detection of Pneumocystis carinii in bronchoalveolar lavage specimens from immunosuppressed patients. Acta Cytol 34:511516.
12. Clinical and Laboratory Standards Institute. 2008. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. CLSI document M27-A3. Clinical and Laboratory Standards Institute, Wayne, PA.
13. Clinical and Laboratory Standards Institute. 2008. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi. CLSI document M38-A2. Clinical and Laboratory Standards Institute, Wayne, PA.
14. Sanati H,, Messer SA,, Pfaller M,, Witt M,, Larsen R,, Espinel-Ingroff A,, Ghannoum M. 1996. Multicenter evaluation of broth microdilution method for susceptibility testing of Cryptococcus neoformans against fluconazole. J Clin Microbiol 34:12801282.
15. Rex JH,, Cooper CRJr,, Merz WG,, Galgiani JN,, Anaissie EJ. 1995. Detection of amphotericin B-resistant Candida isolates in a broth-based system. Antimicrob Agents Chemother 39:906909.
16. Subcommittee on Antifungal Susceptibility Testing (AFST) of the ESCMID European Committee for Antimicrobial Susceptibility Testing (EUCAST). 2008. EUCAST Definitive Document EDef 7.1: method for the determination of broth dilution MICs of antifungal agents for fermentative yeasts. Clin Microbiol Infect 14:398405.
17. Subcommittee on Antifungal Susceptibility Testing (AFST) of the ESCMID European Committee for Antimicrobial Susceptibility Testing (EUCAST). 2008. EUCAST technical note on the method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for conidia-forming moulds. Clin Microbiol Infect 14:982984.
18. Murray MP,, Zinchuk R,, Larone DH. 2005. CHROMagar Candida as the sole primary medium for isolation of yeasts and as a source medium for the rapid-assimilation-of-trehalose test. J Clin Microbiol 43:12101212.
19. Odds FC,, Bernaerts R. 1994. CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species. J Clin Microbiol 32:19231929.
20. Pincus DH,, Orenga S,, Chatellier S. 2007. Yeast identification—past, present, and future methods. Med Mycol 45:971 21.
21. Scognamiglio T,, Zinchuk R,, Gumpeni P,, Larone DH. 2010. Comparison of inhibitory mold agar to Sabouraud dextrose agar as a primary medium for isolation of fungi. J Clin Microbiol 48:19241925.
22. Leeming JP,, Notman FH. 1987. Improved methods for isolation and enumeration of Malassezia furfur from human skin. J Clin Microbiol 25:20172019.
23. Padhye AA,, Ajello L. 1988. Simple method of inducing sporulation by Apophysomyces elegans and Saksenaea vasiformis. J Clin Microbiol 26:18611863.

Tables

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TABLE 1

Commercial sources of yeast chromogenic agar media

Citation: Lindsley M, Snyder J, Atlas R, Larocco M. 2015. Reagents, Stains, and Media: Mycology*, p 1955-1964. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch115

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