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Image of FIGURE 1

(Row 1, left) Gram stain of BAL specimen showing Gram-negative branching septate hyphae of Magnification, approximately ×1,000. doi:10.1128/9781555817381.ch116.f1

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Image of FIGURE 2

(Row 1, right) Gram stain of skin biopsy with Gram-variable branching hyphae of . Magnification, approximately ×1,000. doi:10.1128/9781555817381.ch116.f2

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Image of FIGURE 3

(Row 2, left) Gram stain of skin biopsy with Gram-negative aseptate hyphae of the group. Magnification, approximately ×1,000. doi:10.1128/9781555817381.ch116.f3

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Image of FIGURE 4

(Row 2, right) Gram stain of sputum with showing Gram-positive budding yeast cells with pseudohyphae. doi:10.1128/9781555817381.ch116.f4

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Image of FIGURE 5

(Row 3, left) Gram stain of spleen demonstrating ghost-like budding yeastlike cells with pseudohyphae. Magnification, approximately ×1,000. doi:10.1128/9781555817381.ch116.f5

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Image of FIGURE 6

(Row 3, right) Gram stain of vaginal secretions with Gram-positive budding yeast cells of . doi:10.1128/9781555817381.ch116.f6

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Image of FIGURE 7

(Row 4, left) Gram stain of skin abscess with showing Gram-positive and Gram-variable budding yeast cells of variable size surrounded by amorphous orange halos. doi:10.1128/9781555817381.ch116.f7

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Image of FIGURE 8

(Row 4, right) Parker ink stain of skin lesion showing typical yeast cells and short, stubby hyphae of spp. Magnification, approximately ×400. doi:10.1128/9781555817381.ch116.f8

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Image of FIGURE 9

(Row 1, left) Gomori methenamine silver (GMS) stain of BAL specimen showing dichotomously branching septate hyphae. Magnification, approximately ×400. doi:10.1128/9781555817381.ch116.f9

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Image of FIGURE 10

(Row 1, right) Calcofluor white stain of BAL specimen showing dichotomously branching septate hyphae. Magnification, approximately ×500. doi:10.1128/9781555817381.ch116.f10

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Image of FIGURE 11

(Row 2, left) GMS stain of skin biopsy with spp. showing ribbonlike hyphae. Magnification, approximately ×400. doi:10.1128/9781555817381.ch116.f11

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Image of FIGURE 12

(Row 2, right) Calcofluor white stain of spp. showing ribbonlike hyphae. Magnification, approximately ×500. doi:10.1128/9781555817381.ch116.f12

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Image of FIGURE 13

(Row 3, left) GMS stain of lung biopsy specimen showing with bulb formation at hyphal tip. Magnification, approximately ×1,000. doi:10.1128/9781555817381.ch116.f13

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Image of FIGURE 14

(Row 3, right) H&E stain of lung biopsy specimen showing dichotomously branching septate hyphae (shown at arrow). Magnification, approximately ×400. doi:10.1128/9781555817381.ch116.f14

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Image of FIGURE 15

(Row 1, left) Diff-Quik stain of CSF with . Magnification, approximately ×400. doi:10.1128/9781555817381.ch116.f15

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Image of FIGURE 16

(Row 1, right) India ink preparation of CSF showing encapsulated budding yeast cell of . Magnification, approximately ×400. doi:10.1128/9781555817381.ch116.f16

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Image of FIGURE 17

(Row 2, left) H&E stain of eumycotic mycetoma granule of . Magnification, approximately ×400. doi:10.1128/9781555817381.ch116.f17

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Image of FIGURE 18

(Row 2, right) Toluidine blue stain of BAL specimen showing cysts of . doi:10.1128/9781555817381.ch116.f18

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Image of FIGURE 19

(Row 3, left) Calcofluor white stain of toenail specimen showing arthroconidia and hyphae of . Magnification, approximately ×200. doi:10.1128/9781555817381.ch116.f19

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Image of FIGURE 20

(Row 3, right) Muriform (sclerotic) cells (copper penny) in tissue of chromoblastomycosis. Magnification, ×1,000. doi:10.1128/9781555817381.ch116.f20

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Image of FIGURE 21

(Row 1, left) GMS stain of lymph node showing characteristic cigar-shaped yeast cells of . doi:10.1128/9781555817381.ch116.f21

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Image of FIGURE 22

(Row 1, right) Gram stain of abscess material showing large, broad-based, budding yeast cell with thick refractile wall characteristic of doi:10.1128/9781555817381.ch116.f22

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Image of FIGURE 23

(Row 2, left) GMS stain of lymph node showing blastoconidia of . Magnification, ×625. doi:10.1128/9781555817381.ch116.f23

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Image of FIGURE 24

(Row 2, right) H&E stain of . Large, round, thick-walled spherules (10 to 80 µm in diameter) filled with endospores (2 to 5 µm in diameter). Young spherules have a clear center with peripheral cytoplasm and a prominent thick wall. doi:10.1128/9781555817381.ch116.f24

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Image of FIGURE 25

(Row 3, left) Bright-field photomicrograph of showing multiple budding yeast cells resembling mariner’s wheels. Magnification, ×1,590. doi:10.1128/9781555817381.ch116.f25

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Image of FIGURE 26

(Row 3, right) Wright-Giemsa stain of BAL specimen showing characteristic fission yeast cells of doi:10.1128/9781555817381.ch116.f26

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Characteristic fungal morphology seen by direct examination of clinical specimens

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Methods and stains available for direct microscopic and histological detection of fungi in clinical specimens


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