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Abstract:

The clinical features, diagnosis, and biology of the fungal pathogen , which causes pneumonia in immunocompromised patients and can colonize healthy individuals with an intact immune system.

Citation: Cushion M. 2015. , p 2015-2029. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch118
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Image of FIGURE 1
FIGURE 1

Major developmental forms of (A) Single trophic form; (B) sporocyte (precyst); (C) cyst with four visible spores (intracystic bodies); (D) cyst with three intracystic spores and a spore that has apparently excysted (arrow); (E) cyst with localized thickening of the cell wall (arrow) and eight visible spores. Nomarski Interference Contrast microscopy; magnification ×1,000. doi:10.1128/9781555817381.ch118.f1

Citation: Cushion M. 2015. , p 2015-2029. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch118
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Image of FIGURE 2
FIGURE 2

Proposed life cycle of (A) The primary site of infection is the lung alveoli. Three clusters of alveoli are illustrated. An expanded schematic of an alveolus (box) is shown in panel B. (B) Single alveolus with organisms depicted as a hatched shape attached to the cells lining the alveoli in some areas (type I pneumocytes) and unattached to other alveolar cells (type II pneumocytes). (C) Putative sexual cycle of in the lung alveoli: ( ) opposite mating types fuse and undergo karyogamy resulting in a diploid zygote; ( ) the zygote then undergoes meiosis resulting in four nuclei; ( ) additional postmeiotic mitosis increases the number of nuclei to eight; ( ) the nuclei and mitochondria (not shown) are compartmentalized by invagination of the inner plasma membrane, resulting in eight spores. Spores are released from the ascus (cyst) and presumably enter into the vegetative phase of the cycle. (D) Asexual replication cycle of Trophic forms undergo binary fission after mitotic replication of the nucleus. (Drawn with SmartDraw Suite, ed. 7.3.) doi:10.1128/9781555817381.ch118.f2

Citation: Cushion M. 2015. , p 2015-2029. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch118
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Image of FIGURE 3
FIGURE 3

Morphology and tinctorial characteristics of in clinical samples stained with various stains (magnification, × 960, unless stated otherwise). (A) Calcofluor stain of BAL fluid. Cyst walls with internal thickenings (double comma) are highly fluorescent (color varies with barrier filter used). (B) Rapid Giemsa-like (Diff-Quick) stain of BALF. Thick cluster of mostly trophic forms (2 to 3 μm) with small reddish-purple nuclei and light blue to red-violet cytoplasm. Boundaries of trophic forms are rarely discernible with this stain. Trophic forms overlay each other to produce darker staining blue cytoplasm. Large dark purple host nuclei are admixed in the cluster. (C) Gomori methenamine silver stain (Grocott) of organisms from BALF. Cyst walls and thickenings (double comma) can be observed as well as collapsed, cup shapes and crinkled raisin-like appearance. Note the lack of budding. Trophic forms are not stained with silver-based stains. (D) Papanicolaou’s stain of BALF. Note the distinctive alveolar cast morphology; magnification, ×400. (E) Toluidine blue O stain of in BALF. Cyst walls are stained light purple. The crinkled appearance of the cysts is illustrated with this stain, as well as darker central staining body. Note the lack of budding with this and other cyst wall stains. Trophic forms are not stained. (F) Direct fluorescent antibody stain of organism cluster from BAL. Note apple-green fluorescence distributed unevenly over the cluster, with accumulation on a cyst wall (lower left of cluster). Structures within the cysts are unstained and appear black. doi:10.1128/9781555817381.ch118.f3

Citation: Cushion M. 2015. , p 2015-2029. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch118
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References

/content/book/10.1128/9781555817381.mcm11.ch118
1. Telzak EE,, Cote RJ,, Gold JW,, Campbell SW,, Armstrong D. 1990. Extrapulmonary Pneumocystis carinii infections. Rev Infect Dis 12:380386.
2. Ng VL,, Yajko DM,, Hadley WK. 1997. Extrapulmonary pneumocystosis. Clin Microbiol Rev 10:401418.
3. Morris A,, Norris KA. 2012. Colonization by Pneumocystis jirovecii and its role in disease. Clin Microbiol Rev 25:297317.
4. Redhead SA,, Cushion MT,, Frenkel JK,, Stringer JR. 2006. Pneumocystis and Trypanosoma cruzi: nomenclature and typifications. J Eukaryot Microbiol 53:211.
5. Hibbett DS,, Binder M,, Bischoff JF,, Blackwell M,, Cannon PF,, Eriksson OE,, Huhndorf S,, James T,, Kirk PM,, Lucking R,, Thorsten Lumbsch H,, Lutzoni F,, Matheny PB,, McLaughlin DJ,, Powell MJ,, Redhead S,, Schoch CL,, Spatafora JW,, Stalpers JA,, Vilgalys R,, Aime MC,, Aptroot A,, Bauer R,, Begerow D,, Benny GL,, Castlebury LA,, Crous PW,, Dai YC,, Gams W,, Geiser DM,, Griffith GW,, Gueidan C,, Hawksworth DL,, Hestmark G,, Hosaka K,, Humber RA,, Hyde KD,, Ironside JE,, Koljalg U,, Kurtzman CP,, Larsson KH,, Lichtwardt R,, Longcore J,, Miadlikowska J,, Miller A,, Moncalvo JM,, Mozley-Standridge S,, Oberwinkler F,, Parmasto E,, Reeb V,, Rogers JD,, Roux C,, Ryvarden L,, Sampaio JP,, Schussler A,, Sugiyama J,, Thorn RG,, Tibell L,, Untereiner WA,, Walker C,, Wang Z,, Weir A,, Weiss M,, White MM,, Winka K,, Yao YJ,, Zhang N. 2007. A higher-level phylogenetic classification of the Fungi. Mycol Re. 111:509547.
6. Frenkel JK. 1976. Pneumocystis jiroveci n. sp. from man: morphology, physiology, and immunology in relation to pathology. Natl Cancer Inst Monogr 43:1330.
7. Dei-Cas E,, Chabe M,, Moukhlis R,, Durand-Joly I,, Aliouat eM,, Stringer JR,, Cushion M,, Noel C,, de Hoog GS,, Guillot J,, Viscogliosi E. 2006. Pneumocystis oryctolagi sp. nov., an uncultured fungus causing pneumonia in rabbits at weaning: review of current knowledge, and description of a new taxon on genotypic, phylogenetic and phenotypic bases. FEMS Microbiol Rev 30:853871.
8. Hawksworth DL. 2007. Responsibility in naming pathogens: the case of Pneumocystis jirovecii, the causal agent of pneumocystis pneumonia. Lancet Infect Dis 7:35.
9. Hughes WT. 1982. Natural mode of acquisition for de novo infection with Pneumocystis carinii. J Infect Dis 145:842848.
10. Choukri F,, Menotti J,, Sarfati C,, Lucet JC,, Nevez G,, Garin YJ,, Derouin F,, Totet A. 2010. Quantification and spread of Pneumocystis jirovecii in the surrounding air of patients with Pneumocystis pneumonia. Clin Infect Dis 51:259265.
11. Chapman JR. 2013. Post-transplant Pneumocystis jirovecii pneumonia—a re-emerged public health problem? Kidney Int 84(2):240243.
12. Cushion MT,, Linke MJ,, Ashbaugh A,, Sesterhenn T,, Collins MS,, Lynch K,, Brubaker R,, Walzer PD. 2010. Echinocandin treatment of pneumocystis pneumonia in rodent models depletes cysts leaving trophic burdens that cannot transmit the infection. PLoS One 5:e8524.
13. Cushion MT,, Collins MS,, Linke MJ. 2009. Biofilm formation by Pneumocystis spp. Eukaryot Cell 8:197206.
14. Cushion MT,, Collins MS. 2011. Susceptibility of Pneumocystis to echinocandins in suspension and biofilm cultures. Antimicrob Agents Chemother 55:45134518.
15. Cushion MT,, Smulian AG,, Slaven BE,, Sesterhenn T,, Arnold J,, Staben C,, Porollo A,, Adamczak R,, Meller J. 2007. Transcriptome of Pneumocystis carinii during fulminant infection: carbohydrate metabolism and the concept of a compatible parasite. PLoS One 2:e423.
16. Burgess JW,, Kottom TJ,, Villegas LR,, Lamont JD,, Baden EM,, Ramirez-Alvarado M,, Limper AH. 2009. The Pneumocystis meiotic PCRan1p kinase exhibits unique temperature-regulated activity. Am J Respir Cell Mol Biol 41:714721.
17. Cushion MT,, Linke MJ,, Collins M,, Keely SP,, Stringer JR. 1999. The minimum number of Pneumocystis carinii f. sp. carinii organisms required to establish infections is very low. J Eukaryot Microbiol 46:111S.
18. Soulez B,, Palluault F,, Cesbron JY,, Dei-Cas E,, Capron A,, Camus D. 1991. Introduction of Pneumocystis carinii in a colony of SCID mice. J Protozool 38:123S125S.
19. Cushion MT,, Kaselis M,, Stringer SL,, Stringer JR. 1993. Genetic stability and diversity of Pneumocystis carinii infecting rat colonies. Infect Immun 61:48014813.
20. Vargas SL,, Ponce CA,, Gigliotti F,, Ulloa AV,, Prieto S,, Munoz MP,, Hughes WT. 2000. Transmission of Pneumocystis carinii DNA from a patient with P. carinii pneumonia to immunocompetent contact health care workers. J Clin Microbiol 38:15361538.
21. Medrano FJ,, Montes-Cano M,, Conde M,, de La HC,, Respaldiza N,, Gasch A,, Perez-Lozano MJ,, Varela JM,, Calderon EJ. 2005. Pneumocystis jirovecii in general population. Emerg Infect Dis 11:245250.
22. Sun L,, Huang MJ,, An YJ,, Guo ZZ. 2009. [An epidemiologic study on Pneumocystis pneumonia in non-HIV infected patients in China]. Zhonghua Liu Xing Bing Xue Za Zhi 30:348351.
23. Beard CB,, Carter JL,, Keely SP,, Huang L,, Pieniazek NJ,, Moura IN,, Roberts JM,, Hightower AW,, Bens MS,, Freeman AR,, Lee S,, Stringer JR,, Duchin JS,, Del Rio C,, Rimland D,, Baughman RP,, Levy DA,, Dietz VJ,, Simon P,, Navin TR. 2000. Genetic variation in Pneumocystis carinii isolates from different geographic regions: implications for transmission. Emerg Infect Dis.6:265272.
24. Choukri F,, Aliouat eM,, Menotti J,, Totet A,, Gantois N,, Garin YJ,, Bergeron V,, Dei-Cas E,, Derouin F. 2011. Dynamics of Pneumocystis carinii air shedding during experimental pneumocystosis. J Infect Dis 203:13331336.
25. Yazaki H,, Goto N,, Uchida K,, Kobayashi T,, Gatanaga H,, Oka S. 2009. Outbreak of Pneumocystis jiroveci pneumonia in renal transplant recipients: P. jiroveci is contagious to the susceptible host. Transplantation 88:380385.
26. Fritzsche C,, Riebold D,, Fuehrer A,, Mitzner A,, Klammt S,, Mueller-Hilke B,, Reisinger EC. 2013. Pneumocystis jirovecii colonization among renal transplant recipients. Nephrology 18:382387.
27. Spencer L,, Ukwu M,, Alexander T,, Valadez K,, Liu L,, Frederick T,, Kovacs A,, Morris A. 2008. Epidemiology of Pneumocystis colonization in families. Clin Infect Dis 46:12371240.
28. Peglow SL,, Smulian AG,, Linke MJ,, Pogue CL,, Nurre S,, Crisler J,, Phair J,, Gold JW,, Armstrong D,, Walzer PD. 1990. Serologic responses to Pneumocystis carinii antigens in health and disease. J Infect Dis 161:296306.
29. Pifer LL,, Hughes WT,, Stagno S,, Woods D. 1978. Pneumocystis carinii infection: evidence for high prevalence in normal and immunosuppressed children. Pediatrics 61:3541.
30. Vargas SL,, Hughes WT,, Santolaya ME,, Ulloa AV,, Ponce CA,, Cabrera CE,, Cumsille F,, Gigliotti F. 2001. Search for primary infection by Pneumocystis carinii in a cohort of normal, healthy infants. Clin Infect Dis 32:855861.
31. Kaur N,, Mahl TC. 2007. Pneumocystis jiroveci (carinii) pneumonia after infliximab therapy: a review of 84 cases. Dig Dis Sci 52:14811484.
32. Komano Y,, Harigai M,, Koike R,, Sugiyama H,, Ogawa J,, Saito K,, Sekiguchi N,, Inoo M,, Onishi I,, Ohashi H,, Amamoto F,, Miyata M,, Ohtsubo H,, Hiramatsu K,, Iwamoto M,, Minota S,, Matsuoka N,, Kageyama G,, Imaizumi K,, Tokuda H,, Okochi Y,, Kudo K,, Tanaka Y,, Takeuchi T,, Miyasaka N. 2009. Pneumocystis jiroveci pneumonia in patients with rheumatoid arthritis treated with infliximab: a retrospective review and case-control study of 21 patients. Arthritis Rheum 61:305312.
33. Tanaka M,, Sakai R,, Koike R,, Komano Y,, Nanki T,, Sakai F,, Sugiyama H,, Matsushima H,, Kojima T,, Ohta S,, Ishibe Y,, Sawabe T,, Ota Y,, Ohishi K,, Miyazato H,, Nonomura Y,, Saito K,, Tanaka Y,, Nagasawa H,, Takeuchi T,, Nakajima A,, Ohtsubo H,, Onishi M,, Goto Y,, Dobashi H,, Miyasaka N,, Harigai M. 2012. Pneumocystis jirovecii pneumonia associated with etanercept treatment in patients with rheumatoid arthritis: a retrospective review of 15 cases and analysis of risk factors. Mod Rheumatol 22(6):849858.
34. Maini R,, Henderson KL,, Sheridan EA,, Lamagni T,, Nichols G,, Delpech V,, Phin N. 2013. Increasing Pneumocystis pneumonia, England, UK, 2000–2010. Emerg Infect Dis 19:386392.
35. Radhi S,, Alexander T,, Ukwu M,, Saleh S,, Morris A. 2008. Outcome of HIV-associated Pneumocystis pneumonia in hospitalized patients from 2000 through 2003. BMC Infect Dis 8:118.
36. Walzer PD,, Evans HE,, Copas AJ,, Edwards SG,, Grant AD,, Miller RF. 2008. Early predictors of mortality from Pneumocystis jirovecii pneumonia in HIV-infected patients: 1985–2006. Clin Infect Dis 46:625633.
37. Fisk DT,, Meshnick S,, Kazanjian PH. 2003. Pneumocystis carinii pneumonia in patients in the developing world who have acquired immunodeficiency syndrome. Clin Infect Dis 36:7078.
38. Tellez I,, Barragan M,, Franco-Paredes C,, Petraro P,, Nelson K,, Del Rio C. 2008. Pneumocystis jiroveci pneumonia in patients with AIDS in the inner city: a persistent and deadly opportunistic infection. Am J Med Sci 335:192197.
39. Monnet X,, Vidal-Petiot E,, Osman D,, Hamzaoui O,, Durrbach A,, Goujard C,, Miceli C,, Bouree P,, Richard C. 2008. Critical care management and outcome of severe Pneumocystis pneumonia in patients with and without HIV infection. Crit Care 12:R28.
40. Yoshida Y,, Takahashi Y,, Minemura N,, Ueda Y,, Yamashita H,, Kaneko H,, Mimori A. 2012. Prognosis of pneumocystis pneumonia complicated in patients with rheumatoid arthritis (RA) and non-RA rheumatic diseases. Mod Rheumatol 22:509514.
41. Mofenson LM,, Brady MT,, Danner SP,, Dominguez KL,, Hazra R,, Handelsman E,, Havens P,, Nesheim S,, Read JS,, Serchuck L,, Van Dyke R. 2009. Guidelines for the prevention and treatment of opportunistic Infections among HIV-exposed and HIV-infected children: recommendations from CDC, the National Institutes of Health, the HIV Medicine Association of the Infectious Diseases Society of America, the Pediatric Infectious Diseases Society, and the American Academy of Pediatrics. MMWR 58:1166.
42. Limper AH,, Pottratz ST,, Martin WJ. 1991. Modulation of Pneumocystis carinii adherence to cultured lung cells by a mannose-dependent mechanism. J Lab Clin Med 118:492499.
43. Limper AH. 1991. Parasitic adherence and host responses in the development of Pneumocystis carinii pneumonia. Semin Respir Infect 6:1926.
44. Limper AH,, Standing JE,, Hoffman OA,, Castro M,, Neese LW. 1993. Vitronectin binds to Pneumocystis carinii and mediates organism attachment to cultured lung epithelial cells. Infect Immun 61:43024309.
45. Huang L,, Crothers K. 2009. HIV-associated opportunistic pneumonias. Respirology 14:474485.
46. Madhi SA,, Cutland C,, Ismail K,, O’Reilly C,, Mancha A,, Klugman KP. 2002. Ineffectiveness of trimethoprim-sulfamethoxazole prophylaxis and the importance of bacterial and viral coinfections in African children with Pneumocystis carinii pneumonia. Clin Infect Dis 35:11201126.
47. Punpanich W,, Groome M,, Muhe L,, Qazi SA,, Madhi SA. 2011. Systematic review on the etiology and antibiotic treatment of pneumonia in human immunodeficiency virus-infected children. Pediatr Infect Dis J 30:e192e202.
48. Gajdusek DC. 1957. Pneumocystis carinii; etiologic agent of interstitial plasma cell pneumonia of premature and young infants. Pediatrics 19:543565.
49. Fatti GL,, Zar HJ,, Swingler GH. 2006. Clinical indicators of Pneumocystis jiroveci pneumonia (PCP) in South African children infected with the human immunodeficiency virus. Int J Infect Dis 10:282285.
50. Walzer PD,, Smulian AG,. 2010. Pneumocystis species, p 33773390. In Mandell GL,, Bennet JE,, Dolin R (ed), Mandell, Douglas and Bennet’s Principles and Practice of Infectious Diseases. Churchill Livingstone Elsevier, Philadelphia, PA.
51. Atochina-Vasserman EN,, Gow AJ,, Abramova H,, Guo CJ,, Tomer Y,, Preston AM,, Beck JM,, Beers MF. 2009. Immune reconstitution during Pneumocystis lung infection: disruption of surfactant component expression and function by S-nitrosylation. J Immunol 182:22772287.
52. Qu J,, He L,, Rong Z,, Pan J,, Chen X,, Morrison DC,, Li X. 2001. Alteration of surfactant proteins A and D in bronchoalveolar lavage fluid of Pneumocystis carinii pneumonia. Chin Med J (Engl) 114:11431146.
53. Kanne JP,, Yandow DR,, Meyer CA. 2012. Pneumocystis jiroveci pneumonia: high-resolution CT findings in patients with and without HIV infection. Am J Roentgenol 198:W555W561.
54. Czarniak P,, Zaluska-Lesniewska I,, Zagozdzon I,, Zurowska A. 2013. Difficulties in diagnosing severe Pneumocystis jiroveci pneumonia after rituximab therapy for steroid-dependent nephrotic syndrome. Ped Nephrol 28:987988.
55. Droste A,, Grosse G,, Niedobitek F. 1991. [Extrapulmonary manifestations of Pneumocystis carinii infection in AIDS]. Verh Dtsch Ges Pathol 75:158162.
56. Kovacs JA,, Masur H. 2009. Evolving health effects of Pneumocystis: one hundred years of progress in diagnosis and treatment. JAMA 301:25782585.
57. Kovacs JA,, Gill VJ,, Meshnick S,, Masur H. 2001. New insights into transmission, diagnosis, and drug treatment of Pneumocystis carinii pneumonia. JAMA 286:24502460.
58. Hadley WK,, Ng VL,. 1999. Pneumocystis, p 12001211. In Murray PM,, Baron EJ,, Pfaller MA,, Tenover FC,, Yolken RH (ed), Manual of Clinical Microbiology, 7th ed. ASM Press, Washington, DC.
59. Huang L,, Hecht FM,, Stansell JD,, Montanti R,, Hadley WK,, Hopewell PC. 1995. Suspected Pneumocystis carinii pneumonia with a negative induced sputum examination. Is early bronchoscopy useful? Am J Respir Crit Care Med 151:18661871.
60. Birriel JA,Jr,, Adams JA,, Saldana MA,, Mavunda K,, Goldfinger S,, Vernon D,, Holzman B,, McKey RM,Jr. 1991. Role of flexible bronchoscopy and bronchoalveolar lavage in the diagnosis of pediatric acquired immunodeficiency syndrome-related pulmonary disease. Pediatrics 87:897899.
61. Ng VL,, Gartner I,, Weymouth LA,, Goodman CD,, Hopewell PC,, Hadley WK. 1989. The use of mucolysed induced sputum for the identification of pulmonary pathogens associated with human immunodeficiency virus infection. Arch Pathol Lab Med 113:488493.
62. To KK,, Wong SC,, Xu T,, Poon RW,, Mok KY,, Chan JF,, Cheng VC,, Chan KH,, Hung IF,, Yuen KY. 2013. Use of nasopharyngeal aspirate for diagnosis of pneumocystis pneumonia. J Clin Microbiol 51:15701574.
63. Tsolaki AG,, Miller RF,, Wakefield AE. 1999. Oropharyngeal samples for genotyping and monitoring response to treatment in AIDS patients with Pneumocystis carinii pneumonia. J Med Microbiol 48:897905.
64. Chandra P,, Delaney MD,, Tuazon CU. 1988. Role of special stains in the diagnosis of Pneumocystis carinii infection from bronchial washing specimens in patients with the acquired immune deficiency syndrome. Acta Cytol 32:105108.
65. Mahan CT,, Sale GE. 1978. Rapid methenamine silver stain for Pneumocystis and fungi. Arch Pathol Lab Med 102:351352.
66. Schumann GB,, Swensen JJ. 1991. Comparison of Papanicolaou’s stain with the Gomori methenamine silver (GMS) stain for the cytodiagnosis of Pneumocystis carinii in bronchoalveolar lavage (BAL) fluid. Am J Clin Pathol 95:583586.
67. Keely SP,, Stringer JR. 2009. Complexity of the MSG gene family of Pneumocystis carinii. BMC Genomics 10:367.
68. Elvin K,, Linder E. 1993. Application and staining patterns of commercial anti-Pneumocystis carinii monoclonal antibodies. J Clin Microbiol 31:22222224.
69. Karageorgopoulos DE,, Vouloumanou EK,, Ntziora F,, Michalopoulos A,, Rafailidis PI,, Falagas ME. 2011. beta-D-glucan assay for the diagnosis of invasive fungal infections: a meta-analysis. Clin Infect Dis 52:750770.
70. Wood BR,, Komarow L,, Zolopa AR,, Finkelman MA,, Powderly WG,, Sax PE. 2013. Test performance of blood beta-glucan for Pneumocystis jirovecii pneumonia in patients with AIDS and respiratory symptoms. AIDS 27:967972.
71. Damiani C,, Le Gal S,, Da Costa C,, Virmaux M,, Nevez G,, Totet A. 2013. Combined quantification of pulmonary Pneumocystis jirovecii DNA and serum (1–>3) beta-dglucan for differential diagnosis of Pneumocystis pneumonia and Pneumocystis colonization. J Clin Microbiol 51:33803388.
72. Karageorgopoulos DE,, Qu JM,, Korbila IP,, Zhu YG,, Vasileiou VA,, Falagas ME. 2011. Accuracy of beta-d-glucan for the diagnosis of Pneumocystis jirovecii pneumonia: a meta-analysis. Clin Microbiol Infect 19(1):3949.
73. Alvarez-Martinez MJ,, Miro JM,, Valls ME,, Moreno A,, Rivas PV,, Sole M,, Benito N,, Domingo P,, Munoz C,, Rivera E,, Zar HJ,, Wissmann G,, Diehl AR,, Prolla JC,, de Anta MT,, Gatell JM,, Wilson PE,, Meshnick SR. 2006. Sensitivity and specificity of nested and real-time PCR for the detection of Pneumocystis jiroveci in clinical specimens. Diagn Microbiol Infect Dis 56:153160.
74. McTaggart LR,, Wengenack NL,, Richardson SE. 2012. Validation of the MycAssay Pneumocystis kit for detection of Pneumocystis jirovecii in bronchoalveolar lavage specimens by comparison to a laboratory standard of direct immunofluorescence microscopy, real-time PCR, or conventional PCR. J Clin Microbiol 50:18561859.
75. Dalpke AH,, Hofko M,, Zimmermann S. 2013. Development and evaluation of a real-time PCR assay for detection of Pneumocystis jirovecii on the fully automated BD MAX platform. J Clin Microbiol 51:23372343.
76. Skelly MJ,, Holzman RS,, Merali S. 2008. Sadenosylmethionine levels in the diagnosis of Pneumocystis carinii pneumonia in patients with HIV infection. Clin Infect Dis 46:467471.
77. Skelly M,, Merali S,, Clarkson AB. 2011. Pneumocystis pneumonia and S-adenosylmethionine plasma levels. J Infect 62:490492.
78. de Boer MG,, Gelinck LB,, van Zelst BD,, van de Sande WW,, Willems LN,, van Dissel JT,, de Jonge R,, Kroon FP. 2011. beta-D-glucan and S-adenosylmethionine serum levels for the diagnosis of Pneumocystis pneumonia in HIV-negative patients: a prospective study. J Infect 62:93100.
79. Huang L,, Cattamanchi A,, Davis JL,, den Boon S,, Kovacs J,, Meshnick S,, Miller RF,, Walzer PD,, Worodria W,, Masur H. 2011. HIV-associated Pneumocystis pneumonia. Proc Am Thorac Soc 8:294300.
80. Patel N,, Koziel H. 2004. Pneumocystis jiroveci pneumonia in adult patients with AIDS: treatment strategies and emerging challenges to antimicrobial therapy. Treat Respir Med 3:381397.
81. Neumann S,, Krause SW,, Maschmeyer G,, Schiel X,, von Lilienfeld-Toal M, Infectious Diseases Working Party (AGIHO), German Society of Hematology and Oncology (DGHO). 2013. Primary prophylaxis of bacterial infections and Pneumocystis jirovecii pneumonia in patients with hematological malignancies and solid tumors: guidelines of the Infectious Diseases Working Party (AGIHO) of the German Society of Hematology and Oncology (DGHO). Ann Hematol 92:433442.
82. Barrett MP,, Gemmell CG,, Suckling CJ. 2013. Minor groove binders as anti-infective agents. Pharmacol Ther 139:1223.
83. Fisk M,, Sage EK,, Edwards SG,, Cartledge JD,, Miller RF. 2009. Outcome from treatment of Pneumocystis jirovecii pneumonia with co-trimoxazole. Int J STD AIDS 20:652653.
84. Kauffman CA,, Carver PL. 2008. Update on echinocandin antifungals. Sem Resp Crit Care Med 29:211219.
85. Kamboj M,, Weinstock D,, Sepkowitz KA. 2006. Progression of Pneumocystis jiroveci pneumonia in patients receiving echinocandin therapy. Clin Infect Dis 43:e92e94.
86. Waters L,, Nelson M. 2007. The use of caspofungin in HIV-infected individuals. Expert Opin Investig Drug. 16:899908.
87. Kazanjian P,, Armstrong W,, Hossler PA,, Lee CH,, Huang L,, Beard CB,, Carter J,, Crane L,, Duchin J,, Burman W,, Richardson J,, Meshnick SR. 2001. Pneumocystis carinii cytochrome b mutations are associated with atovaquone exposure in patients with AIDS. J Infect Dis 183:819822.
88. Scott GB,, Hutto C,, Makuch RW,, Mastrucci MT,, O’Connor T,, Mitchell CD,, Trapido EJ,, Parks WP. 1989. Survival in children with perinatally acquired human immunodeficiency virus type 1 infection. N Engl J Med 321:17911796.
89. Wakefield AE,, Pixley FJ,, Banerji S,, Sinclair K,, Miller RF,, Moxon ER,, Hopkin JM. 1990. Amplification of mitochondrial ribosomal RNA sequences from Pneumocystis carinii DNA of rat and human origin. Mol Biochem Parasitol 43:6976.
90. Dumoulin A,, Mazars E,, Seguy N,, Gargallo-Viola D,, Vargas S,, Cailliez JC,, Aliouat EM,, Wakefield AE,, Dei-Cas E. 2000. Transmission of Pneumocystis carinii disease from immunocompetent contacts of infected hosts to susceptible hosts. Eur J Clin Microbiol Infect Dis 19:671678.
91. Lane BR,, Ast JC,, Hossler PA,, Mindell DP,, Bartlett MS,, Smith JW,, Meshnick SR. 1997. Dihydropteroate synthase polymorphisms in Pneumocystis carinii. J Infect Dis 175:482485.

Tables

Generic image for table
TABLE 1

Comparison of stains used to detect

Citation: Cushion M. 2015. , p 2015-2029. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch118
Generic image for table
TABLE 2

PCR detection of : suggested primers and conditions

Citation: Cushion M. 2015. , p 2015-2029. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch118

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