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Chapter 134 : Reagents, Stains, and Media: Parasitology

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Reagents, Stains, and Media: Parasitology, Page 1 of 2

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Abstract:

The evaluation of clinical specimens for ova and parasites in the clinical laboratory can involve the use of direct macroscopic examination of the specimen and microscopic examination of fresh and preserved specimens, as well as culture for some parasitic organisms. These examinations necessitate the use of a variety of stains, reagents, and media. This chapter discusses the most common reagents, stains, and media used for diagnostic parasitology, including directions for making reagents and stains and listings of commercially available reagents and stains.

Citation: Linscott A, Sharp S. 2015. Reagents, Stains, and Media: Parasitology, p 2310-2316. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch134
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References

/content/book/10.1128/9781555817381.mcm11.ch134
1. Garcia LS. 2007. Diagnostic Medical Parasitology, 5th ed, p 978. ASM Press, Washington, DC.
2. Garcia LS. 2007. Diagnostic Medical Parasitology, 5th ed, p 769. ASM Press, Washington, DC.
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4. Garcia LS,, Shimizu RY,, Brewer TC,, Bruckner DA. 1983. Evaluation of intestinal parasite morphology in polyvinyl alcohol preservative: comparison of copper sulfate and mercuric chloride base for use in Schaudinn’s fixative. J Clin Microbiol 17:10921095.
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8. Garcia LS. 2007. Diagnostic Medical Parasitology, 5th ed, p 881909. ASM Press, Washington, DC.
9. Wheatley WB. 1951. A rapid staining procedure for intestinal amoebae and flagellates. Am J Clin Pathol 21:990991.
10. Ignatius R,, Lehmann M,, Miksits K,, Regnath T,, Arvand M,, Engelmann E,, Hahn H,, Wagner J. 1997. A new acid-fast trichrome stain for simultaneous detection of Cryptosporidium parvum and microsporidial species in stool specimens. J Clin Microbiol 35:446449.
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Tables

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TABLE 1

Preservatives used in diagnostic parasitology (intestinal tract specimens)

Citation: Linscott A, Sharp S. 2015. Reagents, Stains, and Media: Parasitology, p 2310-2316. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch134
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TABLE 2

Commercially available collection/transport vials for the recovery of parasites

Citation: Linscott A, Sharp S. 2015. Reagents, Stains, and Media: Parasitology, p 2310-2316. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch134
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TABLE 3

Stains used for parasitic identification

Citation: Linscott A, Sharp S. 2015. Reagents, Stains, and Media: Parasitology, p 2310-2316. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch134
Generic image for table
TABLE 4

Commercially available stains for the detection of parasites

Citation: Linscott A, Sharp S. 2015. Reagents, Stains, and Media: Parasitology, p 2310-2316. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch134
Generic image for table
TABLE 5

Media used for cultivation of parasites

Citation: Linscott A, Sharp S. 2015. Reagents, Stains, and Media: Parasitology, p 2310-2316. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch134

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