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Citation: Novak-Weekley S, Leber A. .
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Image of FIGURE 1

Intestinal amebae of humans. (Top row) Trophozoites. is shown with ingested RBCs. This is the only microscopic finding that allows differentiation of the pathogenic species from the nonpathogenic species . An ameboid form of is rarely seen and is difficult to identify. (Middle row) Cysts. For the central body form is depicted. (Bottom row) Trophozoite nuclei, shown in relative proportion. doi:10.1128/9781555817381.ch140.f1

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Image of FIGURE 2

(Top row) trophozoites. Note the ingested RBCs in the cytoplasm. (Middle row) trophozoites. Note the absence of RBCs in the cytoplasm. (Bottom row) cysts. These cysts cannot be identified to the species level on the basis of morphology. Organisms are stained with Wheatley’s trichrome stain. Courtesy of L. Garcia. doi:10.1128/9781555817381.ch140.f2

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Image of FIGURE 3

on an iodine wet mount preparation. Note the peripheral nuclei and the central body form. Courtesy of L. Garcia. doi:10.1128/9781555817381.ch140.f3

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Image of FIGURE 4

trophozoite (left) and cyst (right). Note the “bull’s-eye” nucleus in the trophozoite and the numerous chromatoidal bars within the cyst. Organisms are stained with Wheatley’s trichrome stain. Courtesy of L. Garcia. doi:10.1128/9781555817381.ch140.f4

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Image of FIGURE 5

(Top row) trophozoite (left; trichrome) and cyst (right; trichrome). Note the large eccentric karyosome in the trophozoite and the chromatoidal bars with sharp pointed ends in the cyst. (Middle row) cyst in iodine (left) and in saline (right). The nuclei are visible; the presence of five or more nuclei is confirmatory for . (Bottom row) cyst (left; trichrome) and cyst (right; trichrome). Both cysts are very distorted and shrunken, although the nuclei can be seen in the cyst on the right. This type of shrinkage is typical of cysts on permanent-stained smears. Courtesy of L. Garcia. doi:10.1128/9781555817381.ch140.f5

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Image of FIGURE 6

(Top row) trophozoites. Note the typical nucleus on the left and the nuclear variation on the right. (Middle row) cysts. Note the typical four nuclei, none of which have peripheral chromatin. (Bottom row) trophozoite (left) and cyst (right). Note the “basket nucleus” in the cyst, along with the large glycogen vacuole. Organisms are stained with Wheatley’s trichrome stain. Courtesy of L. Garcia. doi:10.1128/9781555817381.ch140.f6

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Image of FIGURE 7

Intestinal and urogenital flagellates of humans. (Top row) Trophozoites. is found in urogenital sites; all other flagellates are intestinal. (Bottom row) Cysts. trophozoite is shown; no cyst stage. doi:10.1128/9781555817381.ch140.f7

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Image of FIGURE 8

(Top row) trophozoites. Note the typical nuclei, axonemes, and curved median bodies. (Bottom row) cysts. The cysts on the right are stained with iron-hematoxylin, and those on the left are stained with Wheatley’s trichrome stain. Courtesy of L. Garcia. doi:10.1128/9781555817381.ch140.f8

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Image of FIGURE 9

trophozoites. The trophozoites can have one nucleus (left) or two nuclei (right). No cyst stage is known for this organism. Trophozoites are stained with Wheatley’s trichrome stain. Courtesy of L. Garcia. doi:10.1128/9781555817381.ch140.f9

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Image of FIGURE 10

trophozoites. Note the undulating membrane, which comes about halfway down the body. Although the membrane appears to come down farther on one of the trophozoites (right), this appearance occurs because of the photographic angle. Also, note the axostyle that penetrates the bottom of the organism. No cyst stage is known for this organism. Trophozoites are stained with Giemsa stain. Courtesy of L. Garcia. doi:10.1128/9781555817381.ch140.f10

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Image of FIGURE 11

trophozoite (left) and cyst (right). Note the oral groove (feeding groove) at the right side of the trophozoite (clear area) and the curved fibril (“shepherd’s crook”) in the cyst. Also note the typical pear or lemon shape of the cyst. Organisms are stained with Wheatley’s trichrome stain. Courtesy of L. Garcia. doi:10.1128/9781555817381.ch140.f11

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Image of FIGURE 12

trophozoite (left) and cyst (right). Note the single macronucleus visible in both the trophozoite and the cyst. The much smaller micronucleus is not visible. Organisms are stained with Wheatley’s trichrome stain. Courtesy of L. Garcia. doi:10.1128/9781555817381.ch140.f12

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Intestinal and urogenital amebae, flagellates, and ciliates of humans

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Key features of trophozoites and cysts of common intestinal amebae and

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Sensitivity and specificity of diagnostic tests for amebiasis

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Key features of trophozoites and cysts of common intestinal and urogenital flagellates

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Key features of the ciliate


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