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Chapter 151 : Susceptibility Test Methods: Parasites

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Abstract:

Accurate methods for ascertaining responses of parasites to antiparasitic drugs can prove useful at several levels. They can assist in the clinical management of individual patients, they can yield epidemiologic information that may guide drug use policies and public health interventions, and they offer crucial research tools for the development of new and better drugs. Drug susceptibility tests fall into three broad categories: tests, tests, and molecular tests. tests with patients directly assess the clinical efficacies of existing compounds. Their interpretation is limited by potential interference by factors related to the host or to the environment. tests circumvent these interferences by isolating the parasites from their hosts and investigating them in culture under controlled laboratory conditions, with opportunities for repeated assessments against multiple compounds, including experimental compounds. Molecular tests detect genetic variations that are potentially linked with resistance. Such tests offer unique advantages. PCRs can be performed with minute amounts of nonviable parasite genetic material. They can be run in batches, allowing large-scale epidemiologic studies. Because of their short duration (hours), molecular diagnostic procedures can potentially be used to guide patient management. These different categories of tests provide complementary information. They are discussed for five parasitic diseases, selected for their particular chemotherapeutic challenges: malaria, trichomoniasis, leishmaniasis, African trypanosomiasis, and schistosomiasis.

Citation: Le Bras J, Clain J, Secor W. 2015. Susceptibility Test Methods: Parasites, p 2563-2571. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch151
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Tables

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TABLE 1

Selected antiparasitic agents and susceptibility testing methods

Citation: Le Bras J, Clain J, Secor W. 2015. Susceptibility Test Methods: Parasites, p 2563-2571. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch151

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