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Image of FIGURE 1

(row 1, left) Gram stain (×100) of surgical wound showing elastin fibers. doi:10.1128/9781555817381.ch18.f1.

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Image of FIGURE 2

(row 1, right) Gram stain (×1,000) of sputum showing Curschmann’s spirals. doi:10.1128/9781555817381.ch18.f2.

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Image of FIGURE 3

(row 2, left) Gram stain (×1,000) of vaginal secretions showing clue cells. doi:10.1128/9781555817381.ch18.f3.

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Image of FIGURE 4

(row 2, right) Gram stain (×100) of unacceptable sputum specimen (grossly contaminated with members of the oropharyngeal microbiota) showing >10 SECs per low-power field. doi:10.1128/9781555817381.ch18.f4.

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Image of FIGURE 5

(row 3, left) Gram stain (×100) of acceptable sputum specimen showing <10 SECs per low-power field. doi:10.1128/9781555817381.ch18.f5.

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Image of FIGURE 6

(row 3, right) Gram stain (×100) of urine showing 4+ SECs, indicating gross contamination with vaginal or periurethral secretions and bacteria. doi:10.1128/9781555817381.ch18.f6.

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Image of FIGURE 7

(row 4, left) Gram stain (×1,000) of urine showing PMNs and 4+ Gram-negative bacilli. doi:10.1128/9781555817381.ch18.f7.

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Image of FIGURE 8

(row 4, right) Gram stain (×1,000) of a wound showing PMNs, mixed bacterial morphotypes suggesting aerobic and anaerobic bacteria, and both intra- and extracellular bacteria. This appearance suggests a mixed aerobic and anaerobic abscess or closed-space infection. doi:10.1128/9781555817381.ch18.f8.

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Selection of common clinical specimens for bacterial culture

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Collection, transport, and storage guidelines for microbiological laboratory diagnostic studies

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Suitability of various specimens for anaerobic culture

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Specimen management for infrequently encountered organisms

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Screening specimens requested for routine bacterial culture to ensure quality

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Suggested priority list for accessioning samples received for bacteriology studies

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Recommendations for Gram stain and plating media for bacteriology specimens or organisms

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Usual etiologies of selected infectious disease syndromes

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Recommended blood volumes to obtain for blood cultures

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Diagnosis of BV using a Gram-stained smear of vaginal secretions, by the Vaginal Infection and Prematurity Study Group criteria

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Interpretation of bacterial lower respiratory tract culture results

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Interpretation of urine culture results


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