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Chapter 31 : : Laboratory Characteristics of Slowly Growing Mycobacteria*

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Abstract:

This chapter summarizes the epidemiology, transmission, and clinical significance of the slowly growing mycobacteria, including complex and complex. In addition, laboratory diagnostics for the species-level identification of slowly growing mycobacteria are discussed, including direct examinations and phenotypic and genotypic methods. Strain typing and antimicrobial susceptibility test methods are also included. Updates to this chapter since the 10th edition of this include the addition of recently identified species; discussion of the lipoarabinomannan urinary antigen; and discussions of new molecular methods of identification, including the Cepheid GeneXpert MTB/RIF test, the Speed-Oligo Line Probe Assay, the Seegene multiplex PCR assay, mass spectrometry for and nontuberculous mycobacteria, as well as real-time PCR for detection.

Citation: Simner P, Stenger S, Richter E, Brown-Elliott B, Wallace R, Wengenack N. 2015. : Laboratory Characteristics of Slowly Growing Mycobacteria*, p 570-594. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch31
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Image of FIGURE 1
FIGURE 1

grown on a Löwenstein-Jensen slant for 14 days. Colonies are dry, wrinkled, off-white, and eugenic. doi:10.1128/9781555817381.ch31.f1

Citation: Simner P, Stenger S, Richter E, Brown-Elliott B, Wallace R, Wengenack N. 2015. : Laboratory Characteristics of Slowly Growing Mycobacteria*, p 570-594. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch31
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Image of FIGURE 2
FIGURE 2

on Löwenstein-Jensen slants after 2 weeks of growth before exposure to light (left) and after exposure to light (photochromogenic) (right). doi:10.1128/9781555817381.ch31.f2

Citation: Simner P, Stenger S, Richter E, Brown-Elliott B, Wallace R, Wengenack N. 2015. : Laboratory Characteristics of Slowly Growing Mycobacteria*, p 570-594. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch31
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Image of FIGURE 3
FIGURE 3

grown on 7H10 Middlebrook agar for 10 days. Colonies are yellow and smooth (scotochromogenic). doi:10.1128/9781555817381.ch31.f3

Citation: Simner P, Stenger S, Richter E, Brown-Elliott B, Wallace R, Wengenack N. 2015. : Laboratory Characteristics of Slowly Growing Mycobacteria*, p 570-594. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch31
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Image of FIGURE 4
FIGURE 4

grown on 7H10 Middlebrook agar for 10 days. Colonies are small, colorless, and smooth (nonchromogenic). doi:10.1128/9781555817381.ch31.f4

Citation: Simner P, Stenger S, Richter E, Brown-Elliott B, Wallace R, Wengenack N. 2015. : Laboratory Characteristics of Slowly Growing Mycobacteria*, p 570-594. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch31
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Image of FIGURE 5
FIGURE 5

Line probe assay (GenoType Mycobacterium CM/AS) for the identification of species. CC, conjugate control; UC, universal control; GC, genus-specific control; 4 to 17, specific bands for identification of the species; M, marker line (flags the upper side of the strip). Lines 1 to 6, CM strips; lines 7 and 8, AS strips. First lane, ; second lane, MTBC; third lane, ; fourth lane, ; fifth lane, ; sixth lane, negative control; seventh lane, ; eighth lane, doi:10.1128/9781555817381.ch31.f5

Citation: Simner P, Stenger S, Richter E, Brown-Elliott B, Wallace R, Wengenack N. 2015. : Laboratory Characteristics of Slowly Growing Mycobacteria*, p 570-594. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch31
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Tables

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TABLE 1

Characteristics of currently recognized slowly growing species of NTM

Citation: Simner P, Stenger S, Richter E, Brown-Elliott B, Wallace R, Wengenack N. 2015. : Laboratory Characteristics of Slowly Growing Mycobacteria*, p 570-594. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch31
Generic image for table
TABLE 2

Important properties of selected slowly growing NTM species

Citation: Simner P, Stenger S, Richter E, Brown-Elliott B, Wallace R, Wengenack N. 2015. : Laboratory Characteristics of Slowly Growing Mycobacteria*, p 570-594. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch31
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TABLE 3

Commercially available NAA tests for detection of complex

Citation: Simner P, Stenger S, Richter E, Brown-Elliott B, Wallace R, Wengenack N. 2015. : Laboratory Characteristics of Slowly Growing Mycobacteria*, p 570-594. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch31
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TABLE 4

Phenotypic identification of , , and BCG

Citation: Simner P, Stenger S, Richter E, Brown-Elliott B, Wallace R, Wengenack N. 2015. : Laboratory Characteristics of Slowly Growing Mycobacteria*, p 570-594. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch31
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TABLE 5

Primers for amplification and sequencing of several mycobacterial target genes/sequences

Citation: Simner P, Stenger S, Richter E, Brown-Elliott B, Wallace R, Wengenack N. 2015. : Laboratory Characteristics of Slowly Growing Mycobacteria*, p 570-594. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch31

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