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Chapter 33 : Approaches to the Identification of AerobicGram-Negative Bacteria*

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Approaches to the Identification of AerobicGram-Negative Bacteria*, Page 1 of 2

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Abstract:

This chapter describes the approaches used to identify Gram-negative rods, with emphasis on the greater difficulty in identifying non-glucose-fermenting organisms. We amplify what was presented in the 9th edition of this by presenting a scheme to identify these organisms that is centered around three enzymatic activities, i.e., oxidase, trypsin (benzyl-arginine arylamidase or benzyl-arginine aminopeptidase), and pyrrolidonyl aminopeptidase. These enzymatic reactions are fast and easy to interpret, and they are stable markers in almost all taxa discussed; i.e., there are few species for which these tests yield variable intraspecies results.

Citation: Wauters G, Vaneechoutte M. 2015. Approaches to the Identification of AerobicGram-Negative Bacteria*, p 613-634. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch33
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References

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1. Martin R,, Riley PS,, Hollis DG,, Weaver RE,, Krichevsky MI. 1981. Characterization of some groups of gram-negative nonfermentative bacteria by the carbon source alkalinization technique. J Clin Microbiol 14:3947.
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9. Wauters G,, Van Bosterhaut B,, Janssens M,, Verhaegen J. 1998. Identification of Corynebacterium amycolatum and other nonlipophilic fermentative corynebacteria of human origin. J Clin Microbiol 36:14301432.
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Tables

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TABLE 1

Dichotomous identification algorithm for Gram-negative bacteria with poor or no growth on SBA

Citation: Wauters G, Vaneechoutte M. 2015. Approaches to the Identification of AerobicGram-Negative Bacteria*, p 613-634. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch33
Generic image for table
TABLE 2

Dichotomous identification algorithm for aerobically growing Gram-negative bacteria which form purple or pink colonies on SBA

Citation: Wauters G, Vaneechoutte M. 2015. Approaches to the Identification of AerobicGram-Negative Bacteria*, p 613-634. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch33
Generic image for table
TABLE 3

Dichotomous identification algorithm for aerobically growing Gram-negative bacteria fermenting glucose (facultative anaerobes)

Citation: Wauters G, Vaneechoutte M. 2015. Approaches to the Identification of AerobicGram-Negative Bacteria*, p 613-634. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch33
Generic image for table
TABLE 4

Dichotomous identification algorithm for aerobically growing Gram-negative nonfermenters (strict aerobes)

Citation: Wauters G, Vaneechoutte M. 2015. Approaches to the Identification of AerobicGram-Negative Bacteria*, p 613-634. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch33
Generic image for table
TABLE 5

Overview of biochemical characteristics of the Gram-negative nonfermenters

Citation: Wauters G, Vaneechoutte M. 2015. Approaches to the Identification of AerobicGram-Negative Bacteria*, p 613-634. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch33

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