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Chapter 61 : General Approaches to Identification of , , and Obligate Intracellular Bacteria

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General Approaches to Identification of , , and Obligate Intracellular Bacteria, Page 1 of 2

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Abstract:

, , and obligate intracellular bacteria (, , , , , , and ) differ from others by several characteristics. This includes the lack of efficiency for characterization using Gram stains and, except for and species and to a limited extent and , the obligatory requirement for intracellular growth within a eukaryotic host cell. Thus, the most frequently used tests in clinical microbiology laboratories, Gram staining and axenic culture, are inefficient for detection and diagnosis. As a result, this has often been accomplished by an alternative staining method, such as Giemsa and Wright stains of clinical samples, by histopathologic or direct fluorescent-antibody analysis of biopsy samples; or most often by detection of antibody responses using a variety of serologic tests. The application of nucleic acid tests is now the standard practice for some of these bacteria. Additionally, improvements in axenic and cell culture methods have significantly enhanced their detection and the ability to render laboratory-based diagnoses for the diseases that they cause.

Citation: Stephen Dumler J. 2015. General Approaches to Identification of , , and Obligate Intracellular Bacteria, p 1082-1087. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch61
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Tables

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TABLE 1

Epidemiology and clinical diseases associated with , , , , , , , , and infections

Citation: Stephen Dumler J. 2015. General Approaches to Identification of , , and Obligate Intracellular Bacteria, p 1082-1087. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch61
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TABLE 2

Diagnostic tests for , , , , , , , , and

Citation: Stephen Dumler J. 2015. General Approaches to Identification of , , and Obligate Intracellular Bacteria, p 1082-1087. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch61

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