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Chapter 76 : Susceptibility Test Methods: Mycobacteria, , and Other Actinomycetes

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Abstract:

This chapter has four major sections. First is a brief review of antimicrobial agents used to treat mycobacterial infections. This is followed by a discussion of methods used to test susceptibility of complex to first- and second-line agents. Methods described are agar proportion, rapid broth systems, broth microdilution, and molecular techniques, which are being used with increasing frequency. The next topic is susceptibility testing of nontuberculous mycobacteria by broth microdilution, focusing on complex, , , and the rapidly growing mycobacteria. The last section covers susceptibility testing of species by broth microdilution and includes a brief discussion of other aerobic actinomycetes.

Citation: Woods G, Lin S, Desmond E. 2015. Susceptibility Test Methods: Mycobacteria, , and Other Actinomycetes, p 1356-1378. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch76
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Tables

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TABLE 1

Antimicrobial agents recommended for primary treatment of common mycobacterial infections

Citation: Woods G, Lin S, Desmond E. 2015. Susceptibility Test Methods: Mycobacteria, , and Other Actinomycetes, p 1356-1378. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch76
Generic image for table
TABLE 2

Genes associated with drug resistance in

Citation: Woods G, Lin S, Desmond E. 2015. Susceptibility Test Methods: Mycobacteria, , and Other Actinomycetes, p 1356-1378. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch76
Generic image for table
TABLE 3

Test concentrations of antimycobacterial agents against complex

Citation: Woods G, Lin S, Desmond E. 2015. Susceptibility Test Methods: Mycobacteria, , and Other Actinomycetes, p 1356-1378. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch76
Generic image for table
TABLE 4

Guidelines for selection of the dilution of a specimen concentrate prior to inoculation of 7H10 medium for susceptibility testing using the direct method

Citation: Woods G, Lin S, Desmond E. 2015. Susceptibility Test Methods: Mycobacteria, , and Other Actinomycetes, p 1356-1378. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch76
Generic image for table
TABLE 5

Antimycobacterial agents, interpretative criteria, and quality control recommendations for testing complex by broth microdilution

Citation: Woods G, Lin S, Desmond E. 2015. Susceptibility Test Methods: Mycobacteria, , and Other Actinomycetes, p 1356-1378. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch76
Generic image for table
TABLE 6

Secondary antimycobacterial agents and MIC values indicating resistance for testing . and other slowly growing nontuberculous mycobacteria (excluding complex)

Citation: Woods G, Lin S, Desmond E. 2015. Susceptibility Test Methods: Mycobacteria, , and Other Actinomycetes, p 1356-1378. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch76
Generic image for table
TABLE 7

Broth microdilution interpretive criteria for rapidly growing mycobacteria

Citation: Woods G, Lin S, Desmond E. 2015. Susceptibility Test Methods: Mycobacteria, , and Other Actinomycetes, p 1356-1378. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch76
Generic image for table
TABLE 8

Broth microdilution breakpoints for

Citation: Woods G, Lin S, Desmond E. 2015. Susceptibility Test Methods: Mycobacteria, , and Other Actinomycetes, p 1356-1378. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch76

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