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Image of FIGURE 1

Tomography of A2 HRSV virions. Virion morphology ranges from completely filamentous (A) to completely spherical (C) with intermediate forms (B and D) that have some tubularly curved parts but are otherwise spherically curved. Spherical particles are highly deformable when in the proximity of other particles and the membrane proximal to the neighboring particle is free of glycoprotein spikes (E). (F) Virions in panels A through E are illustrated schematically in alphabetical order. Black arrows: side views of the RNP; white arrows: top views of the RNP; green arrows: secondary density layer under the membrane in a spherical particle (bar, 100 nm). Tomographic slices are 3.8 nm thick. Reprinted from with permission. doi:10.1128/9781555817381.ch86.f1

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Image of FIGURE 2

Distribution of RSV peak month by geographic zone ( = 96 locations). The black histogram represents observations while the red curve illustrates the fit of a Gaussian density kernel. Reprinted from with permission. doi:10.1128/9781555817381.ch86.f2

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Image of FIGURE 3

Microscopic detection of RSV and hMPV. (A) RSV-infected HEp-2 cells have fused, forming large syncytia. (B) Direct immunofluorescence of RSV on a nasopharyngeal swab specimen. (C) Indirect immunofluorescence of HMPV-infected LLC-MK2 centrifugation culture stained with monoclonal antibody MAb-8 ( ). Images courtesy of http://www.virology.org/hpphoto3.html (A) and David Ferguson and Marie Landry (B and C). doi:10.1128/9781555817381.ch86.f3

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Image of FIGURE 4

Seasonal distribution of HMPV infections. HMPV-positive specimens by week in Cincinnati Children’s Hospital, University of Rochester Medical Center, and Vanderbilt University Medical Center from 2003 through 2009. Reprinted from with permission. doi:10.1128/9781555817381.ch86.f4

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Commercial RSV and hMPV DFA reagents and their performance

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Main commercially available RSV rapid antigen products and their performance characteristics

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Commercial NAATs for detection and identification of RSV and hMPV


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