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Chapter 11.17 : Neutrophil Function Whole-Blood Flow Cytometric Test for Leukocyte Adhesion Deficiency

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Neutrophil Function Whole-Blood Flow Cytometric Test for Leukocyte Adhesion Deficiency, Page 1 of 2

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Abstract:

Normal neutrophil function is of great importance in the host defense against bacterial and fungal infections. As neutrophils recognize, adhere to, and phagocytose an invading microbe they generate an oxidative burst, which kills the pathogen. In the leukocyte adhesion deficiency (LAD) states, there are defects in the ability of neutrophils to adhere to both the vascular endothelium and opsonized microorganisms due to the absence or reduced expression of a group of cell surface leukocyte adhesion markers. This group of surface glycoproteins includes LFA-1 (CD11b/CD18), Cr3 (CD11b/CD18), and P150,95 (CD11c/CD18). Patients with this deficiency manifest recurrent infections involving the skin, subcutaneous tissues, middle ear, and oropharyngeal mucosa. In the assay described here, neutrophils are stimulated with phorbol 12 myristate 13 acetate (PMA) and stained with CD11b plus corresponding isotypic monoclonal antibody. Cellular fluorescence is measured on resting and stimulated neutrophils using flow cytometry.

Citation: Garcia L. 2010. Neutrophil Function Whole-Blood Flow Cytometric Test for Leukocyte Adhesion Deficiency, p 250-253. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch11.17
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References

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1. Centers for Disease Control and Prevention. 1997. Revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency virus (HIV). MMWR Morb. Mortal. Wkly. Rep. 46(RR-2):129.
2. Hassan, N. F.,, D. E. Campbell,, and S. D. Douglas. 1988. Phorbol myristate acetate oxidation of 2′7′ dichlorofluorescein by neutrophils from patients with chronic granulatomatous disease. J. Leukoc. Biol. 43:317322.
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4. O’Gorman, M. R. G.,, and A. C. Mcnally. 1993. A rapid whole blood lysis technique for diagnosis of moderate or severe leukocyte adhesion deficiency. Ann. N. Y. Acad. Sci. 677:427430.
5. Todd, R. F.,, and D. R. Freyer. 1988. The CD11/CD18 leukocyte glycoprotein deficiency. Hematol. Oncol. Clin. N. Am. 2:1331.

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