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Chapter 11.4 : Urinary Antigen Detection for Legionella spp.
Patients with Legionnaires' disease excrete soluble serogroup-specific Legionella antigen into their urine. Urinary antigen tests to detect Legionnaires' disease were developed by several groups soon after the 1976 Philadelphia, PA, outbreak of Legionnaires' disease ( 3 , 37 ) and then further refined by Kohler et al. and other groups ( 5 , 16 , 27 – 30 , 34 – 36 ). The antigen being detected has never been extensively purified, but it is known that it is resistant to boiling, is trypsin resistant, has a molecular mass of approximately 10 kDa, and is most likely a lipopolysaccharide ( 29 , 39 ).