1887

Chapter 3.13 : Wound Cultures

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Preview this chapter:
Zoom in
Zoomout

Wound Cultures, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555817435/9781555815271_Chap3_13-1.gif /docserver/preview/fulltext/10.1128/9781555817435/9781555815271_Chap3_13-2.gif

Abstract:

A wide variety of microorganisms that reside on the skin and mucous membranes of the body, as well as those found in the environment, can cause skin and soft tissue infections. These organisms enter the body through breaks in the skin or mucous membranes, through wounds made by trauma or bites (exogenous) or as a complication of surgery or foreign-body implants (endogenous), or they can be spread through the vascular system (hematogenous).

Citation: Garcia L. 2010. Wound Cultures, p 441-462. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.13
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

Figures

Image of Figure 3.13.1-1
Figure 3.13.1-1

Illustration of sterile-scalpel method of homogenization of tissue.

Citation: Garcia L. 2010. Wound Cultures, p 441-462. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.13
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Figure 3.13.1-2
Figure 3.13.1-2

Illustration of mortar-and-pestle method of homogenization of tissue.

Citation: Garcia L. 2010. Wound Cultures, p 441-462. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.13
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Figure 3.13.1-3
Figure 3.13.1-3

Illustration of stomacher method of homogenization of tissue.

Citation: Garcia L. 2010. Wound Cultures, p 441-462. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.13
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Figure 3.13.1-4
Figure 3.13.1-4

Illustration of tissue-grinding kit method of homogenization of tissue.

Citation: Garcia L. 2010. Wound Cultures, p 441-462. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.13
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Figure 3.13.1-5
Figure 3.13.1-5

Initial evaluation of positive wound cultures for organisms growing aerobically. : For lymph nodes, perform all work using a biological safety cabinet.

Citation: Garcia L. 2010. Wound Cultures, p 441-462. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.13
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Figure 3.13.1-6
Figure 3.13.1-6

Algorithm to rapidly detect aerobic and facultatively aerobic microorganisms usually considered significant, even in low numbers or in mixed cultures. See procedures 3.18.1 and 3.18.2 for other tests needed to confirm suspected identifications.

Citation: Garcia L. 2010. Wound Cultures, p 441-462. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.13
Permissions and Reprints Request Permissions
Download as Powerpoint
Image of Untitled
Untitled

Citation: Garcia L. 2010. Wound Cultures, p 441-462. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.13
Permissions and Reprints Request Permissions
Download as Powerpoint

References

/content/book/10.1128/9781555817435.chap3.13
1. Bowler, P. G.,, B. I. Duerden,, and D. G. Armstrong. 2001. Wound microbiology and associated approaches to wound management. Clin. Microbiol. Rev. 14:244269.
2.Clinical and Laboratory Standards Institute. 2004. Quality Assurance for Commercially Prepared Microbiological Culture Media, 3rd ed. Approved standard M22-A3. Clinical and Laboratory Standards Institute, Wayne, PA.
3. Levine, N. S.,, R. B. Lindberg,, A. D. Mason, Jr.,, and B. A. Pruitt, Jr. 1976. The quantitative swab culture and smear: a quick, simple method for determining the number of viable aerobic bacteria on open wounds. J. Trauma 16:8994.
4. Magee, C.,, B. Haury,, G. Rodeheaver,, J. Fox,, M. T. Edgerton,, and R. F. Edlieh. 1977. A rapid technique for quantitating wound bacterial count. Am. J. Surg. 133:760762.
5. Morris, A. J.,, S. J. Wilson,, C. E. Marx,, M. L. Wilson,, S. Mirrett,, and L. B. Reller. 1995. Clinical impact of bacteria and fungi recovered only from broth cultures. J. Clin. Microbiol. 33:161165.
6. Rinehold, C. E.,, D. J. Nickolai,, T. E. Piccinni,, B. A. Byford,, M. K. York,, and G. F. Brooks. 1988. Evaluation of broth media for routine culture of cerebrospinal and joint fluid specimens. Am. J. Clin. Pathol. 89:671674.
7. Roelofsen, E.,, M. van Leeuwen,, G. J. Meijer-Severs,, M. H. Wilkinson,, and J. E. Degener. 1999. Evaluation of the effects of storage in two different swab fabrics and under three different transport conditions on recovery of aerobic and anaerobic bacteria. J. Clin. Microbiol. 37:30413043.
8. Silletti, R. P.,, E. Ailey,, S. Sun,, and D. Tang. 1997. Microbiologic and clinical value of primary broth cultures of wound specimens collected with swabs. J. Clin. Microbiol. 35:20032006.
9. Bartlett, R. C. 1974. Medical Microbiology: Quality Cost and Clinical Relevance. John Wiley & Sons, Inc., New York, NY.
10. Bartlett, R. C.,, M. Mazens-Sullivan,, J. Z. Tetreault,, S. Lobel,, and J. Nivard. 1994. Evolving approaches to management of quality in clinical microbiology. Clin. Microbiol. Rev. 7:5588.
11. Hindiyeh, M.,, V. Acevedo,, and K. C. Carroll. 2001. Comparison of three transport systems (Starplex StarSwab II, the new Copan Vi-Pak Amies agar gel collection and transport swabs, and BBL Port-A-Cul) for maintenance of anaerobic and fastidious aerobic organisms. J. Clin. Microbiol. 39:377380.
12. Miller, J. M. 1996. A Guide to Specimen Management in Clinical Microbiology, 2nd ed. ASM Press, Washington, DC.
13. Perry, J. L. 1997. Assessment of swab transport systems for aerobic and anaerobic organism recovery. J. Clin. Microbiol. 35:12691271.
14. Sharp, S. E. 1999. Algorithms for wound specimens. Clin. Microbiol. Newsl. 21:118120.
15. Wilson, M. 2005. Microbial Inhabitants of Humans: Their Ecology and Role in Health and Disease. Cambridge University Press, New York, NY.
1. Bornside, G. H.,, and B. B. Bornside. 1979. Comparison between moist swab and tissue biopsy methods for quantitation of bacteria in experimental incisional wounds. J. Trauma 19:103105.
2. Bowler, P. G.,, B. I. Duerden,, and D. G. Armstrong. 2001. Wound microbiology and associated approaches to wound management. Clin. Microbiol. Rev. 14:244269.
3.Clinical and Laboratory Standards Institute. 2004. Quality Assurance for Commercially Prepared Microbiological Culture Media, 3rd ed. Approved standard M22-A3. Clinical and Laboratory Standards Institute, Wayne, PA.
4. Edlich, R. F.,, G. T. Rodeheaver,, T. R. Stevenson,, C. M. Magee,, J. G. Thaeker,, and M. T. Edgerton. 1977. Management of the contaminated wound. Compr. Ther. 3:6774.
5. Lawrence, J. C.,, and H. A. Lilly. 1972. A quantitative method for investigating the bacteriology of skin: its application to burns. Br. J. Exp. Pathol. 53:550558.
6. Loebl, E. C.,, J. A. Marvin,, E. L. Heek,, P. W. Curreri,, and C. R. Baxter. 1974. The use of quantitative biopsy cultures in bacteriologic monitoring of burn patients. J. Surg. Res. 16:15.
7. Magee, C.,, B. Haury,, G. Rodeheaver,, J. Fox,, M. T. Edgerton,, and R. F. Edlich. 1977. A rapid technique for quantitating wound bacterial count. Am. J. Surg. 133:760762.
8. Rodeheaver, G. T.,, J. Hiebert,, R. F. Edlich,, and M. Spengler. 1980. Practical bacteriologic monitoring of the burn patient. Curr. Concepts Trauma Care 3:815.
24. Heggers, J. R.,, and M. C. Robson (ed.). 1991. Quantitative Bacteriology: Its Role in the Armamentarium of the Surgeon. CRC Press, Boca Raton, FL.

Tables

Generic image for table
Table 3.13.1-1

Common types of superficial and deep wounds and abscesses

Citation: Garcia L. 2010. Wound Cultures, p 441-462. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.13
Generic image for table
Table 3.13.1-2

Aerobic and anaerobic isolates from acute and chronic infections

Data revised from reference . Wounds include abscesses, postsurgical wounds, bites, ulcers, and pressure sores.

causes skin ulceration and should be ruled when spp. are isolated in this clinical context.

Citation: Garcia L. 2010. Wound Cultures, p 441-462. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.13
Generic image for table
Table 3.13.1-3

Commonly encountered superficial and deep-wound/abscess, drainage, and tissue infections

Table 3.13.1-2 for a complete listing of aerobic and anaerobic isolates from acute and chronic wound/abscess and tissue infections.

See procedure 3.5 and Table 3.5-2 for drainages that are acceptable for culture.

Citation: Garcia L. 2010. Wound Cultures, p 441-462. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.13
Generic image for table
Table 3.13.1-4

Examples of mixed wound culture reporting

Revised from reference .

Caution must be used in using the presence of absence of PMNs to report the presence of certain pathogens from mixed wound cultures. Clostridia and other anaerobes produce phospholipases and lipases, and some aerobes (e.g., , group A , , and spp.) produce phospholipases that can destroy host cells, so a reduced or absent number of PMNs may be found in the direct specimen Gram smear.

Citation: Garcia L. 2010. Wound Cultures, p 441-462. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.13

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error