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Chapter 3.17 : Biochemical Tests for the Identification of Aerobic Bacteria

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Abstract:

Acetamide agar is used to test an organism's ability to utilize acetamide by deamidation. The medium contains acetamide as the sole carbon source and inorganic ammonium salts as the sole source of nitrogen. Growth is indicative of a positive test for acetamide utilization. When the bacterium metabolizes acetamide by the enzymatic action of an acylamidase, the ammonium salts are broken down to ammonia, which increases alkalinity. The shift in pH turns the bromthymol blue indicator in the medium from green to blue, indicative of a positive test. Assimilation of acetamide will result in a yellow color and should not by mistaken for a positive result ( ). In general, deamidation is limited to only a few organisms. This medium is recommended for differentiating from other non-glucose-fermenting, gram-negative rods.

Citation: Garcia L. 2010. Biochemical Tests for the Identification of Aerobic Bacteria, p 503-642. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.17
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Figure 3.17.8-1

Demonstration of positive CAMP and reverse CAMP test, using streak and CAMP disk.

Citation: Garcia L. 2010. Biochemical Tests for the Identification of Aerobic Bacteria, p 503-642. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.17
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Tables

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Citation: Garcia L. 2010. Biochemical Tests for the Identification of Aerobic Bacteria, p 503-642. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.17
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Citation: Garcia L. 2010. Biochemical Tests for the Identification of Aerobic Bacteria, p 503-642. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.17
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±, with or without.

Citation: Garcia L. 2010. Biochemical Tests for the Identification of Aerobic Bacteria, p 503-642. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.17
Generic image for table
Untitled

Citation: Garcia L. 2010. Biochemical Tests for the Identification of Aerobic Bacteria, p 503-642. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.17
Generic image for table
Untitled

NA, not applicable because the organism will not grow on the medium.

Citation: Garcia L. 2010. Biochemical Tests for the Identification of Aerobic Bacteria, p 503-642. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch3.17