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Chapter 4.13 : Suggestions for a Practical Scheme for the Workup of Anaerobic Cultures

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Abstract:

When a specimen is sent to the laboratory in an appropriate container and from an appropriate site with a request for anaerobe testing, a Gram stain should be performed and reported. The results of the Gram stain should direct the culture workup to be done as well as provide information to the clinician and the laboratory about the quality of the specimen and the potential for mixed aerobic and anaerobic floras. All anaerobe requests should receive a simultaneous aerobic culture, and these should be worked up in concert before the report is finalized. The scheme for identification of anaerobes can take on many models, depending upon the extent of workup required by the clinicians and the amount of expertise and time that can be allotted to their identification by the clinical microbiologist. Certainly, all single isolates from sterile sites should be completely worked up to genus and species name as much as is possible. It is less consistent among laboratories as to how much is fully identified in nonsterile sites or what should be identified when a “sterile” site, such as ascites fluid or abscess or even tissues, contains a mixed flora, including aerobic and anaerobic organisms, in a quantity of more than three organisms.

Citation: Garcia L. 2010. Suggestions for a Practical Scheme for the Workup of Anaerobic Cultures, p 790-793. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch4.13
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Figure 4.13-1

Workup of isolates from sterile sites. EYA, egg yolk agar.

Citation: Garcia L. 2010. Suggestions for a Practical Scheme for the Workup of Anaerobic Cultures, p 790-793. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch4.13
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References

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Tables

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Table 4.13-1

Identification of isolates of anaerobic bacteria in nonsterile sites

Citation: Garcia L. 2010. Suggestions for a Practical Scheme for the Workup of Anaerobic Cultures, p 790-793. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch4.13

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