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Chapter 4.4 : Examination of Primary Culture Plates for Anaerobic Bacteria

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Abstract:

The goal of processing primary culture plates is to isolate significant anaerobic organisms present in the original specimen for identification and when susceptibility testing is indicated. The original Gram stain of the specimen is critical. At that time all morphological types should be carefully described and recorded. When evaluating culture plates, all morphotypes observed in the original Gram stain should match.

Citation: Garcia L. 2010. Examination of Primary Culture Plates for Anaerobic Bacteria, p 703-708. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch4.4
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Figures

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Figure 4.4-1

Procedure for the examination of primary culture plates for anaerobes. Symbols: *, use any other suitable enriched primary media that contain vitamin K and hemin and that allow good growth of anaerobes; †, use EYA if clostridia are suspected from Gram stain or from nature of clinical specimen. KVLB, kanamycin-vancomycin-laked blood agar.

Citation: Garcia L. 2010. Examination of Primary Culture Plates for Anaerobic Bacteria, p 703-708. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch4.4
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Citation: Garcia L. 2010. Examination of Primary Culture Plates for Anaerobic Bacteria, p 703-708. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch4.4
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References

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1. Dowell, V. R., Jr.1989. Procedures for Isolation and Characterization of Anaerobic Bacteria. Centers for Disease Control, Atlanta, GA.
2. Engelkirk, P. G.,, J. Duben-Engelkirk,, and V. R. Dowell, Jr. 1992. Principles and Practice of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA.
3. Forbes, B.A.,, D.F. Sahm,, and A. S. Weissfeld (ed.). 2007. Bailey and Scott's Diagnostic Bacteriology, 12th ed. Mosby Elsevier, St. Louis, MO.
4. Holdeman, L.V.,, E. P. Cato,, and W. E. C. Moore. 1977. Anaerobe Laboratory Manual, 4th ed., p. 24, 122, 149. Virginia Polytechnic Institute and State University, Blacksburg.
5. Jousimies-Somer, H. R.,, P. Summanen,, D. M. Citron,, E. J. Baron,, H. M. Wexler,,and S. M. Finegold. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
6. Mangels, J. I.,, M. E. Cox,, and L. H. Lindberg. 1984. Methanol fixation—an alternative to heat fixation of smears before staining. Diagn. Microbiol. Infect. Dis. 2:129137.
7. Murray, P. R.,, E. J. Baron,, J. H. Jorgensen,, M. L. Landry,, and M. A. Pfaller (ed.).2007. Manual of Clinical Microbiology, 9th ed. ASM Press, Washington, DC.

Tables

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Table 4.4-1

Anaerobic organism clues from primary culture plates, and use of supplemental media

Citation: Garcia L. 2010. Examination of Primary Culture Plates for Anaerobic Bacteria, p 703-708. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch4.4
Generic image for table
Table 4.4-2

Special-potency antimicrobial agent disks for the identification of anaerobic bacteria

Adapted with permission from H. R. Jousimies-Somer, P. Summanen, D. M. Citron, E. J. Baron, H. M. Wexler, and S. M. Finegold, 6th ed., 2002, Star Publishing Co., Belmont, CA.

R, resistant; S, susceptible; V, variable.

Exceptions: rare strains of spp. and spp. may be vancomycin resistant.

Citation: Garcia L. 2010. Examination of Primary Culture Plates for Anaerobic Bacteria, p 703-708. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch4.4

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