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Chapter 4.7 : Commercial Kit Overnight Biochemical Systems for the Identification of Anaerobes

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Abstract:

Biochemical systems for identification of an anaerobe rely on the metabolic break-down of substrates and the production of end products during the growth of the isolated organism. There are at least two commercially packaged kit systems that fit this method for the identification of commonly isolated anaerobic bacteria in the clinical microbiology laboratory when species identification is needed: the API 20A (bioMérieux, Inc.) and the AN Microplate (Biolog) ( ) ( Table 4.7-1 ). Both rely on overnight incubation of the test card before reading. The API 20A is manually filled and read; the AN Microplate is automatically filled but manually (visually) read. The API 20A uses 16 carbohydrates; its indicator system is bromcresol purple that turns yellow at a pH of 6.8 when a positive reaction occurs. The AN Microplate has a database of 361 anaerobes that are exposed to 95 preselected carbon sources for specific identification. The Minitek (BD Biosciences) kit was described in the last two editions of this handbook, but there were no references on the BD website that indicated that these systems were being manufactured any longer. Procedure 4.8 describes the BD Crystal ANR ID, which had largely taken over the role of the older Minitek panels.

Citation: Garcia L. 2010. Commercial Kit Overnight Biochemical Systems for the Identification of Anaerobes, p 737-739. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch4.7
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References

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1. Carroll, K. C.,, and M. P. Weinstein,. 2007. Manual and automated systems for detection and identification of microorganisms, p. 192217. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. L. Landry,, and M. A. Pfaller (ed.), Manual of Clinical Microbiology, 9th ed. ASM Press, Washington, DC.
2. Citron, D. M.,, I. R. Poxton,, and E. J. Baron,. 2007. Bacteroides, Porphyromonas, Prevotella, Fusobacterium, and other anaerobic gram-negative rods, p. 911932. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. L. Landry,, and M. A. Pfaller (ed.), Manual of Clinical Microbiology, 9th ed. ASM Press, Washington, DC.
3. Forbes, B. A.,, D. F. Sahm,, and A. S. Weissfeld (ed.). 2007. Bailey and Scott's Diagnostic Bacteriology, 12th ed. Mosby Elsevier, St. Louis, MO.
4. Head, C. B.,, and S. Ratnam. 1988. Comparison of API ZYM system with API-Ident, API 20A, Minitek Anaerobe II, and RapID-ANA systems for identification of Clostridium difficile. J. Clin. Microbiol. 26:144146.
5. Karachewski, N. O.,, E. L. Busch,, and C. L. Wells. 1985. Comparison of PRAS II, RapID ANA, and API 20A systems for identification of anaerobic bacteria. J. Clin. Microbiol. 21:122126.
6. Moore, H. B.,, V. L. Sutter,, and S. M. Finegold. 1975. Comparison of three procedures for biochemical testing of anaerobic bacteria. J. Clin. Microbiol. 1:1522.

Tables

Generic image for table
Table 4.7-1

Characteristics of manual biochemical systems

bioMérieux, Inc.

Biolog.

Citation: Garcia L. 2010. Commercial Kit Overnight Biochemical Systems for the Identification of Anaerobes, p 737-739. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch4.7
Generic image for table
Table 4.7-2

Recommended QC strains

Citation: Garcia L. 2010. Commercial Kit Overnight Biochemical Systems for the Identification of Anaerobes, p 737-739. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch4.7

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