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Chapter 4.9 : Rapid Biochemical Tests (4 Hours or Less) for the Identification of Anaerobes

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Abstract:

Many anaerobic isolates may be identified to the genus and species levels using a variety of preformed-enzyme tests or other rapid biochemical tests. In some in-stances, tests for identification beyond the level that can be attained with the various spot tests described in procedure 4.6 are needed. Presumptive or definitive identification of anaerobes is possible by using individual biochemical tests or a combination of preformed enzymatic manifestations.

Citation: Garcia L. 2010. Rapid Biochemical Tests (4 Hours or Less) for the Identification of Anaerobes, p 745-755. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch4.9
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References

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1. Jousimies-Somer, H. R.,, P. Summanen,, D. M. Citron,, E. J. Baron,, H. M. Wexler,, and S. M. Finegold. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
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11. Moncla, B. J.,, P. Braham,, L. K. Rabe,, and S. L. Hillier. 1991. Rapid presumptive identificationof black-pigmented gram-negative anaerobic bacteria by using 4-methylumbelliferone derivatives. J. Clin. Microbiol. 29:19551958.
12. Hudspeth, M. K.,, S. H. Gerardo,, D. M. Citron,,and E. J. C. Goldstein. 1997. Growth characteristics and a novel method for identification (theWee-Tab System) of Porphyromonas species isolated from infected dog and cat bite wounds in humans. J. Clin. Microbiol. 35:24502453.
13. Jousimies-Somer, H. R.,, P. Summanen,, D. M. Citron,, E. J. Baron,, H. M. Wexler,, and S. M. Finegold. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
14. Key Scientific Products. Wee-Tabs package insert. Key Scientific Products, Round Rock, TX.
15. Mangels, J. I.,, I. Edvalson,, and M. Cox. 1993.Rapid presumptive identification of Bacteroides fragilis group organisms with use of 4-methylumbelliferone-derivative substrates. Clin. Infect. Dis.16(Suppl. 4):S319S321.
16. Moncla, B. J.,, P. Braham,, L. K. Rabe,, and S.L. Hillier. 1991. Rapid presumptive identification of black-pigmented gram-negative anaerobic bacteria by using 4-methylumbelliferone derivatives. J. Clin. Microbiol. 29:19551958.

Tables

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Summary of single or combination rapid enzymatic tests for anaerobes

Citation: Garcia L. 2010. Rapid Biochemical Tests (4 Hours or Less) for the Identification of Anaerobes, p 745-755. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch4.9

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