1887

Chapter 5.1 : Disk Diffusion Test

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Preview this chapter:
Zoom in
Zoomout

Disk Diffusion Test, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555817435/9781555815271_Chap5_1-1.gif /docserver/preview/fulltext/10.1128/9781555817435/9781555815271_Chap5_1-2.gif

Abstract:

A standardized inoculum of bacteria is swabbed onto the surface of a Mueller-Hinton agar (MHA) plate. Filter paper disks impregnated with antimicrobial agents are placed on the agar. After over-night incubation, the diameter of the zone of inhibition is measured around each disk. By referring to the tables in the NCCLS disk diffusion standard ( ), a qualitative report of susceptible, intermediate, or resistant is obtained.

Citation: Garcia L. 2010. Disk Diffusion Test, p 10-24. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.1
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

References

/content/book/10.1128/9781555817435.chap5.1
1.NCCLS. 2003. Performance Standards for Antimicrobial Disk Susceptibility, 8th ed. Approved standard M2-A8. NCCLS, Wayne, Pa.
2. Barry, A. L.,, M. D. Coyle,, C. Thornsberry,, E.H. Gerlach,, and R. W. Hawkinson. 1979. Methods of measuring zones of inhibition with Bauer-Kirby disk susceptibility test. J. Clin. Microbiol. 10:885889.
3. Bauer, A. W.,, W. M. M. Kirby,, J. C. Sherris,, and M. Turck. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45:493496.
4. Bradford, P. A. 2001. Extended-spectrum β-lac-tamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin. Microbiol. Rev. 14:933951.
5. Jorgensen, J. H., and M. J. Ferraro. 2000. Antimicrobial susceptibility testing: special needs for fastidious organisms and difficult-to-detect resistance mechanisms. Clin. Infect. Dis. 30:799808.
6. Jorgensen, J. H., 2003. Susceptibility test methods: dilution and disk diffusion methods. p. 1108 1127. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed. ASM Press, Washington, D.C.
7. Miller, J. M.,, C. Thornsberry,, and C. N. Baker. 1984. Disk diffusion susceptibility test trouble-shooting guide. Lab Med. 15:183185.
1.NCCLS. 2003. Performance Standards for Antimicrobial Disk Susceptibility, 8th ed. Approved standard M2-A8. NCCLS, Wayne, Pa.
1.NCCLS. 2003. Performance Standards for Antimicrobial Disk Susceptibility, 8th ed. Approved standard M2-A8. NCCLS, Wayne, Pa.
1.NCCLS. 2003. Performance Standards for Antimicrobial Disk Susceptibility, 8th ed. Approved standard M2-A8. NCCLS, Wayne, Pa.
1.NCCLS. 2003. Performance Standards for Antimicrobial Disk Susceptibility, 8th ed. Approved standard M2-A8. NCCLS, Wayne, Pa.
1.NCCLS. 2003. Performance Standards for Antimicrobial Disk Susceptibility, 8th ed. Approved standard M2-A8. NCCLS, Wayne, Pa.

Tables

Generic image for table
Untitled

Citation: Garcia L. 2010. Disk Diffusion Test, p 10-24. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.1
Generic image for table

Troubleshooting guide for disk diffusion susceptibility testing,

Reprinted from . 1984. Disk diffusion susceptibility test troubleshooting guide. 183–185, with permission of the American Society of Clinical Pathologists.

Record and note all errors.

Citation: Garcia L. 2010. Disk Diffusion Test, p 10-24. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.1
Generic image for table

Troubleshooting guide for disk diffusion susceptibility testing,

Reprinted from . 1984. Disk diffusion susceptibility test troubleshooting guide. 183–185, with permission of the American Society of Clinical Pathologists.

Record and note all errors.

Citation: Garcia L. 2010. Disk Diffusion Test, p 10-24. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.1
Generic image for table

Quick reference list for performing disk diffusion tests

Disk diffusion testing is not standardized by the NCCLS for organisms other than those in this table. If testing is required, perform by MIC method. Abbreviations: DCS, direct colony suspension—standardize to McFarland 0.5 turbidity standard; DCS/LPG, direct colony suspension or log-phase growth—standardize to McFarland 0.5 turbidity standard; GC, GC agar with defined supplement; LPG, log-phase growth.

All incubation temperatures are 35°C.

And other penicillinase-stable penicillins.

Citation: Garcia L. 2010. Disk Diffusion Test, p 10-24. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.1
Generic image for table
Untitled

Citation: Garcia L. 2010. Disk Diffusion Test, p 10-24. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.1
Generic image for table

Disk Diffusion Susceptibility Testing Quality Control

Acceptable ranges of accuracy are given in NCCLS. 2003. . Thirteenth informational supplement M100-S13 (M2). NCCLS, Wayne, Pa.

Citation: Garcia L. 2010. Disk Diffusion Test, p 10-24. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch5.1

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error